Panax notoginseng is a rare medicinal and edible plant widely used for multi-treatments,and in this study,a polysaccharide Pnp was prepared from P.notoginseng,and its structure and biology activities were investigated...Panax notoginseng is a rare medicinal and edible plant widely used for multi-treatments,and in this study,a polysaccharide Pnp was prepared from P.notoginseng,and its structure and biology activities were investigated.Results showed that the molecular weight of Pnp was 78.6 kDa,and Pnp mainly consisted of→2,4)-α-L-Rhap-(1→,→5)-α-L-Araf-(1→and→4)-α-D-Galp-(1→residues with acetyl groups.Furthermore,the macrophages were activated the immunologic function after TLR2 and MR recognizing Pnp.Besides,Pnp could be durative utilized by the probiotic Lactobacillus plantarum and the account of viable cells kept at 9.5 log CFU/mL for 72h.The hydrophobicity and the short-chain fatty acids levels(especially butyric acid)of L.plantarum were enhanced by Pnp,which indicated that Pnp exerted beneficial effects on the host health.The results laid a solid foundation for the exploitation of Pnp as a nutraceutical and functional candidate in the food industry.展开更多
Background： Mitocbondrial DNA （mtDNA） content measured by different techniques cannot be compared between studies, and age- and tissue-related control values are hardly available. In the present study, we aimed to ...Background： Mitocbondrial DNA （mtDNA） content measured by different techniques cannot be compared between studies, and age- and tissue-related control values are hardly available. In the present study, we aimed to establish the nonllal reference range of mtDNA copy number in the Chinese population. Methods： Two healthy cohorts of 200 Chinese minors （0.1 18.0 years） and 200 adults （18.0-88.0 years） were recruited. Then, they were further categorized into eight age groups. The absolute mtDNA copy number per cell was measured by a quantitative real-time polymerase chain reaction. We subsequently used this range to evaluate mtDNA content in tbur patients （0.5-4.0 years） with molecularly proven mitochondrial depletion syndromes （MDSs） and 83 cases of mitochondrial disease patients harboring the m.3243A〉G mutation. Results： The reference range ofmtDNA copy number in peripheral blood was 175-602 copies/cell （mean： 325 copies/cell） in minors and 164 500 copies/cell （mean： 287 copies/cell） in adults. There was a decreasing trend in mtDNA copy number in blood with increasing age, especially in 0-2-year-old and 〉50-year-old donors. The mean mtDNA copy number level among the mitochondrial disease patients with m.3243A〉G mutation was significantly higher than that ofhealtby controls. The intDNA content ofPOLG, DGUOK, TK2, and SUCLA2 genes in blood samples from MDS patients was reduced to 25%, 38%, 32%, and 24%, respectively. Conclusions： We primarily establish the refeerence intervals of mtDNA copy number, which might contribute to the clinical diagnosis and monitoring of mitochondrial disease.展开更多
基金the Southwest Minzu University Research Startup Funds(RQD2021078)the Open Project Program of the Key Laboratory of Brewing Molecular Engineering of China Light Industry(BME-202201)+3 种基金the Open Project Program of Panxi Crops Research and Utilization Key Laboratory of Sichuan Province(SZKF2217)Sichuan Tianfu Talents Project(1306306002)Natural Science Foundation of Sichuan province(2023NSFSC5863)Na-tional Natural Science Foundation of China(32001675)for partially funding this work.
文摘Panax notoginseng is a rare medicinal and edible plant widely used for multi-treatments,and in this study,a polysaccharide Pnp was prepared from P.notoginseng,and its structure and biology activities were investigated.Results showed that the molecular weight of Pnp was 78.6 kDa,and Pnp mainly consisted of→2,4)-α-L-Rhap-(1→,→5)-α-L-Araf-(1→and→4)-α-D-Galp-(1→residues with acetyl groups.Furthermore,the macrophages were activated the immunologic function after TLR2 and MR recognizing Pnp.Besides,Pnp could be durative utilized by the probiotic Lactobacillus plantarum and the account of viable cells kept at 9.5 log CFU/mL for 72h.The hydrophobicity and the short-chain fatty acids levels(especially butyric acid)of L.plantarum were enhanced by Pnp,which indicated that Pnp exerted beneficial effects on the host health.The results laid a solid foundation for the exploitation of Pnp as a nutraceutical and functional candidate in the food industry.
基金This study was supported by grants from the National Natural Science Foundation of China （No. 81271256 and No. 81471153） and Beijing Municipal Science and Technology Commission （No. Z 131107002213062）.
文摘Background： Mitocbondrial DNA （mtDNA） content measured by different techniques cannot be compared between studies, and age- and tissue-related control values are hardly available. In the present study, we aimed to establish the nonllal reference range of mtDNA copy number in the Chinese population. Methods： Two healthy cohorts of 200 Chinese minors （0.1 18.0 years） and 200 adults （18.0-88.0 years） were recruited. Then, they were further categorized into eight age groups. The absolute mtDNA copy number per cell was measured by a quantitative real-time polymerase chain reaction. We subsequently used this range to evaluate mtDNA content in tbur patients （0.5-4.0 years） with molecularly proven mitochondrial depletion syndromes （MDSs） and 83 cases of mitochondrial disease patients harboring the m.3243A〉G mutation. Results： The reference range ofmtDNA copy number in peripheral blood was 175-602 copies/cell （mean： 325 copies/cell） in minors and 164 500 copies/cell （mean： 287 copies/cell） in adults. There was a decreasing trend in mtDNA copy number in blood with increasing age, especially in 0-2-year-old and 〉50-year-old donors. The mean mtDNA copy number level among the mitochondrial disease patients with m.3243A〉G mutation was significantly higher than that ofhealtby controls. The intDNA content ofPOLG, DGUOK, TK2, and SUCLA2 genes in blood samples from MDS patients was reduced to 25%, 38%, 32%, and 24%, respectively. Conclusions： We primarily establish the refeerence intervals of mtDNA copy number, which might contribute to the clinical diagnosis and monitoring of mitochondrial disease.
