Intestinal macrophages are essential players in intestinal inflammation and intestinal immune homeostasis.Intestinal macrophages have the ability to polarize into two distinct phenotypes based on various environmental...Intestinal macrophages are essential players in intestinal inflammation and intestinal immune homeostasis.Intestinal macrophages have the ability to polarize into two distinct phenotypes based on various environmental signals.These phenotypes include the typically activated pro-inflammatory M1 phenotype and the alternatively activated anti-inflammatory M2 phenotype.Under normal circumstances,intestinal macrophages prevent inflammatory damage to the gut.However,when genetic and environmental factors influence the polarization of intestinal macrophages,it can lead to an imbalance in M1/M2 macrophage activation and subsequently an imbalance in the control of intestinal inflammation.It transforms physiological inflammation into pathological intestinal damage.In patients with ulcerative colitis-associated cancer(UC-CRC),intestinal inflammatory disorders are closely associated with intestinal M1/M2 macrophage polarization imbalance.Consequently,restoring the polarization equilibrium of M1/M2 macrophages might be an evidence of traditional Chinese medicine in the treatment of UC-CRC,the pivotal role o effective measure to prevent and treat UC-CRC.This paper aims to examine the clinicalf macrophage polarization in UC-CRC pathogenesis,and the potential mechanisms of traditional Chinese medicine in regulating macrophage polarization to treat UC-CRC.Our goal is to provide novel insights into the clinical practice,basic research,and drug development of UC-CRC.展开更多
Traditional Medicine Research has retracted this article upon the request of the editor-in-chief and academic committee.On June 5,2023,we received a complaint regarding possible picture plagiarism within the article,w...Traditional Medicine Research has retracted this article upon the request of the editor-in-chief and academic committee.On June 5,2023,we received a complaint regarding possible picture plagiarism within the article,which promoted the decision to retract this article.Following a comprehensive examination,it was determined that the content depicted in this article's pictures was identical to that found in another published paper.Consequently,the editorial office determined that retracting the article was necessary.展开更多
Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoct...Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoction were searched by The TCMSP and Uniprot database and the disease genes of diarrhea were obtained from GeneCard and diagenetic databases.The network software venny 2.1.0 was used to screen the intersection targets of Zhishi Daozhi decoction in treating diarrhea.STRING database was used to construct protein interaction network,Metascape database analysis platform for gene ontology enrichment and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis.Using Cytoscape 3.9.1 software to map the“Chinese herbal medicine-active ingredient-target”network and perform network topology to identify the core genes.Selecting core genes and critical components for molecular docking.Results:203 active ingredients and 259 target genes were obtained from Zhishi Daozhi decoction,206 genes were cross-linked with the disease gene control after de-duplication,1001 entries meeting the screening criteria were obtained after gene ontology enrichment analysis,and 169 entries were obtained after Kyoto Gene and Genome Encyclopedia enrichment analysis,in which the biological processes were mainly enriched in the cellular responses to lipids,hormones,radiation,growth factors,and cell fractionation.The Kyoto Gene and Genome Encyclopedia pathway enrichment analysis suggested that it mainly covers the cancer pathway,hepatitis B,cellular senescence,IL-17 signaling pathway,PI3K-Akt signaling pathway,etc.The molecular docking results showed that the vital active ingredients,such as lignocaine and quercetin,with better binding activity to the vital core targets,such as MMP3,AKT1,and CCL2.Conclusion:The active ingredients such as quercetin and luteolin in Zhishi Daozhi decoction may act through signaling pathways such as IL-17 signaling pathway,PI3K-Akt signaling pathway,TGF-βsignaling pathway,and other pathways,and act on the critical core targets such as MMP3,AKT1,HSP90AA1,FOS,CCL2,and TGFB1 to enhance intestinal barrier function,reduce the inflammatory response and regulate intestinal flora,thus achieving the purpose of treating diarrhea.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.82274600,No.81774451 and No.82104501)the Natural Science Foundation of Guangdong Province(No.2017A030313827)+1 种基金The Basic and Applied Basic Research Project of Guangzhou Municipal Science and Technology Bureau(No.202102080485)Science and Technology Innovation Special Topic of Maoming City(No.2022S014).
文摘Intestinal macrophages are essential players in intestinal inflammation and intestinal immune homeostasis.Intestinal macrophages have the ability to polarize into two distinct phenotypes based on various environmental signals.These phenotypes include the typically activated pro-inflammatory M1 phenotype and the alternatively activated anti-inflammatory M2 phenotype.Under normal circumstances,intestinal macrophages prevent inflammatory damage to the gut.However,when genetic and environmental factors influence the polarization of intestinal macrophages,it can lead to an imbalance in M1/M2 macrophage activation and subsequently an imbalance in the control of intestinal inflammation.It transforms physiological inflammation into pathological intestinal damage.In patients with ulcerative colitis-associated cancer(UC-CRC),intestinal inflammatory disorders are closely associated with intestinal M1/M2 macrophage polarization imbalance.Consequently,restoring the polarization equilibrium of M1/M2 macrophages might be an evidence of traditional Chinese medicine in the treatment of UC-CRC,the pivotal role o effective measure to prevent and treat UC-CRC.This paper aims to examine the clinicalf macrophage polarization in UC-CRC pathogenesis,and the potential mechanisms of traditional Chinese medicine in regulating macrophage polarization to treat UC-CRC.Our goal is to provide novel insights into the clinical practice,basic research,and drug development of UC-CRC.
文摘Traditional Medicine Research has retracted this article upon the request of the editor-in-chief and academic committee.On June 5,2023,we received a complaint regarding possible picture plagiarism within the article,which promoted the decision to retract this article.Following a comprehensive examination,it was determined that the content depicted in this article's pictures was identical to that found in another published paper.Consequently,the editorial office determined that retracting the article was necessary.
基金supported by the science and technology innovation particular topic of Maoming City(No.2021S0047)science and technology innovation particular topic of Maoming City(No.2022S0014).
文摘Background:To explore the mechanism of action of Zhishi Daozhi decoction in treating diarrhea using network pharmacology and molecular docking.Methods:The pharmacological components and targets of Zhishi Daozhi decoction were searched by The TCMSP and Uniprot database and the disease genes of diarrhea were obtained from GeneCard and diagenetic databases.The network software venny 2.1.0 was used to screen the intersection targets of Zhishi Daozhi decoction in treating diarrhea.STRING database was used to construct protein interaction network,Metascape database analysis platform for gene ontology enrichment and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis.Using Cytoscape 3.9.1 software to map the“Chinese herbal medicine-active ingredient-target”network and perform network topology to identify the core genes.Selecting core genes and critical components for molecular docking.Results:203 active ingredients and 259 target genes were obtained from Zhishi Daozhi decoction,206 genes were cross-linked with the disease gene control after de-duplication,1001 entries meeting the screening criteria were obtained after gene ontology enrichment analysis,and 169 entries were obtained after Kyoto Gene and Genome Encyclopedia enrichment analysis,in which the biological processes were mainly enriched in the cellular responses to lipids,hormones,radiation,growth factors,and cell fractionation.The Kyoto Gene and Genome Encyclopedia pathway enrichment analysis suggested that it mainly covers the cancer pathway,hepatitis B,cellular senescence,IL-17 signaling pathway,PI3K-Akt signaling pathway,etc.The molecular docking results showed that the vital active ingredients,such as lignocaine and quercetin,with better binding activity to the vital core targets,such as MMP3,AKT1,and CCL2.Conclusion:The active ingredients such as quercetin and luteolin in Zhishi Daozhi decoction may act through signaling pathways such as IL-17 signaling pathway,PI3K-Akt signaling pathway,TGF-βsignaling pathway,and other pathways,and act on the critical core targets such as MMP3,AKT1,HSP90AA1,FOS,CCL2,and TGFB1 to enhance intestinal barrier function,reduce the inflammatory response and regulate intestinal flora,thus achieving the purpose of treating diarrhea.
