Biomineralization has garnered significant attention in the field of wastewater treatment due to its notable cost reduction compared to conventional methods.The reinjection water from oilfields containing an exceeding...Biomineralization has garnered significant attention in the field of wastewater treatment due to its notable cost reduction compared to conventional methods.The reinjection water from oilfields containing an exceedingly high concentration of calcium and ferric ions will pose amajor hazard in production.However,the utilization of biomineralization for precipitating these ions has been scarcely investigated due to limited tolerance among halophiles towards such extreme conditions.In this study,free and immobilized halophiles Virgibacillus dokdonensis were used to precipitate these ions and the effects were compared,at the same time,biomineralizationmechanisms and mineral characteristicswere further explored.The results showthat bacterial concentration and carbonic anhydrase activitywere higher when additionally adding ferric ion based on calcium ion;the content of protein,polysaccharides,deoxyribonucleic acid and humic substances in the extracellular polymers also increased compared to control.Calcium ions were biomineralized into calcite and vaterite with mul-tiple morphology.Due to iron doping,the crystallinity and thermal stability of calcium carbonate decreased,the content of O-C=O,N-C=OandC-O-PO_(3) increased,the stable carbon isotope values became much more negative,andβ-sheet in minerals disappeared.Higher calcium concentrations facilitated ferric ion precipitation,while ferric ions hindered calcium precipitation.The immobilized bacteria performed better in ferric ion removal,with a precipitation ratio exceeding 90%.Free bacteria performed better in calcium removal,and the precipitation ratio reached a maximum of 56%.This research maybe provides some reference for the co-removal of calcium and ferric ions from the oilfield wastewater.展开更多
Lumpy skin disease(LSD)is a highly contagious disease caused by lumpy skin disease virus(LSDV)in bovines.Rapid and accurate diagnosis is very important to controll it.However,current commercial detection kits need to ...Lumpy skin disease(LSD)is a highly contagious disease caused by lumpy skin disease virus(LSDV)in bovines.Rapid and accurate diagnosis is very important to controll it.However,current commercial detection kits need to be improved in terms of sensitivity or specificity.This study aimed to develop a novel diagnostic competitive enzyme-linked immunosorbent assay(cELISA)based on the newly identified antigen gene LSDV034.The rLSDV034 protein was identified as a potential diagnostic antigen,and it was expressed,purified,and used to immunize BALB/c mice.Using laboratory-prepared indirect ELISA(iELISA),the positive cell lines were screened,and their blocking activity was further verified by competitive ELISA(cELISA).The cell line,1H7,was chosen to produce mouse ascites,which were purified for a monoclonal antibody(mAb,5.395 mg/mL).The heavy chain type of the 1H7 mAb was identified as IgG1a,and its light chain subtype was identified as κ.Furthermore,cELISA was developed to detect bovine serum antibodies,with rLSDV034(4μg/mL)as the coating antigen and HRP-1H7 mAb(1:300)as the competitive antibody.The cutoff value of cELISA was 55%,based on 32 negative bovine serum samples.The analytical sensitivity was 1:8,and no cross-reaction was detected with bovine viral diarrhea virus(BVDV),infectious bovine rhinotracheitis virus(IBRV),Pasteurella multocida(P.multocida),or Mycoplasma bovis(M.bovis)from the serum samples.The diagnostic sensitivity and specificity of cELISA were 98.46%(95%confidence interval,CI:91.7–100)and 100%(95%CI:89.1–100),respectively,based on the analysis of 30 LSDV-infected bovine serum samples,35 GTPV-vaccinated samples,and 32 negative samples.The overall coincidence of the cELISA with the virus neutralization test(VNT)reached 98.97%(95%CI:94.4–100).Furthermore,we used cELISA to analyze 230 clinical bovine serum samples(including 59 infected and 171 vaccinated samples)and found that the serum positivity rates of the immunized samples(on d 60 postimmunization)and infected samples were 77.78%(95%CI:70.8–83.8%)and 71.19%(95%CI:57.9–82.2),respectively.These results indicate that the developed cELISA is promising for detecting serum antibodies in naturally infected or vaccinated cattle.展开更多
This study investigates the unsteady flow characteristics of shale oil reservoirs during the depletion development process,with a particular focus on production behavior following fracturing and shut-in stages.Shale r...This study investigates the unsteady flow characteristics of shale oil reservoirs during the depletion development process,with a particular focus on production behavior following fracturing and shut-in stages.Shale reservoirs exhibit distinctive production patterns that differ from traditional oil reservoirs,as their inflow performance does not conform to the classic steady-state relationship.Instead,production is governed by unsteady-state flow behavior,and the combined effects of thewellbore and choke cause the inflowperformance curve to evolve dynamically over time.To address these challenges,this study introduces the concept of a“Dynamic IPR curve”and develops a dynamic production analysis method that integrates production time,continuity across multi-stage state fields,and the interactions between tubing flow and choke flow.This method provides a robust framework to characterize the attenuation trend of reservoir productivity and to accurately describe wellbore flow behavior.By applying the dynamic IPR approach,the study overcomes the limitations of conventional methods,which are unable to capture the temporal variations inherent in shale reservoir production.The proposed methodology offers a theoretical foundation for improved production forecasting,optimization of choke size,and analysis of wellbore tubing characteristics,thereby supporting more effective operational decision-making across different stages of shale reservoir development.展开更多
A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and...A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and laccase-like)and electrochemical activity.CuO@3-APBA nanoparticles(CuO@3-APBA NPs)were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid(3-APBA)in CuO NPs.3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions.Then,a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme.For the first signal,CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene(TMB)to turn the solution from colorless to blue in the presence of H_(2)O_(2)(absorbance at 652 nm).For the second signal,CuO@3-APBA can catalyze the oxidation of substrates(2,4-dichlorophenol and 4-aminoantipyrine)and turn the solution from colorless to pink(absorbance at 510 nm).For the third signal,electrochemical oxidation peak of copper ion from Cu^(+)to Cu^(2+)of Cu O@3-APBA was recorded by differential pulse voltammetry,which was used to determine the OM.The sensing platform exhibited a wide linear range(0.0000316-100.000000 ng/mL)with a low detection limit(0.0105 pg/mL),as well as showed advantages,such as satisfactory reproducibility,good stability,and excellent selectivity.The assay has the potential applications for ultrasensitive detection of allergen in foods.展开更多
Alcohol oxidation is a widely used green chemical reaction.The reaction process produces flammable and explosive hydrogen,so the design of the reactor must meet stringent safety requirements.Based on the limited exper...Alcohol oxidation is a widely used green chemical reaction.The reaction process produces flammable and explosive hydrogen,so the design of the reactor must meet stringent safety requirements.Based on the limited experimental data,utilizing the traditional numerical method of computational fluid dynamics(CFD)to simulate the gas-liquid two-phase flow reactor can mitigate the risk of danger under varying working conditions.However,the calculation process is highly time-consuming.Therefore,by integrating process simulation,computational fluid dynamics,and deep learning technologies,an intelligent hybrid chemical model based on machine learning was proposed to expedite CFD calculations,enhance the prediction of flow fields,conversion rates,and concentrations inside the reactor,and offer insights for designing and optimizing the reactor for the alcohol oxidation system.The results show that the hybrid model based on the long and short-term memory neural network achieves 99.8%accuracy in conversion rate prediction and 99.9%accuracy in product concentration prediction.Through validation,the hybrid model is accelerated by about 360 times compared with instrumental analysis in conversion rate prediction and about 45 times compared with CFD calculation in concentration distribution prediction.This hybrid model can quickly predict the conversion rate and product concentration distribution in the gas-liquid two-phase flow reactor and provide a model reference for fast prediction and accurate control in the actual chemical production process.展开更多
A novel hydroxy late-rich functionalized biosorbent by grafting citric acid on the decrystallized Dicranopteris dichotoma stem(DDS) was prepared.The obtained g-DDS-3 exhibits good hydrophilicity,thermal stability and ...A novel hydroxy late-rich functionalized biosorbent by grafting citric acid on the decrystallized Dicranopteris dichotoma stem(DDS) was prepared.The obtained g-DDS-3 exhibits good hydrophilicity,thermal stability and superior adsorption performance for Ce^(3+).The effects of citric acid dosage,pH and initial Ce^(3+)concentration on the adsorption experiments were investigated.The maximum adsorption capacity(Langmuir model) of Ce^(3+)by g-DDS-3 is 131.0 mg/g at pH of 7.0,which surpasses that of most previously reported biomass-based materials.Adsorption kinetic results indicate that the adsorption process conforms to the pseudo-second-order model and can achieve equilibrium quickly.By analyzing the adsorption mechanism,we find that carboxyl groups are uniformly distributed on the surface of g-DDS-3 post modification,which provides abundant adsorption sites,and the adsorption is primarily driven by the ion exchange between Ce^(3+)and carboxyl groups protons.In addition,the adsorption-desorption experiments suggest that the g-DDS-3 has excellent recyclability since the adsorption capacity still remain above 90% after five cycles.These findings prove that carboxylate-rich modified D.dichotoma has great potential for application in the effective separation and recovery of Ce^(3+)from leaching tailings.展开更多
Molecular dynamics(MD)simulation is employed to investigate the deformation behavior under various loading paths and strain rates of nanocrystalline magnesium(NC Mg)with[0001]texture.Atomic-scale structural evolution ...Molecular dynamics(MD)simulation is employed to investigate the deformation behavior under various loading paths and strain rates of nanocrystalline magnesium(NC Mg)with[0001]texture.Atomic-scale structural evolution of NC Mg was performed under uniaxial and biaxial loadings.In tension process,compression twins and basal slip dominate,while the compression process is dominated by tension twins.The activation mechanism of twinning is highly sensitive to the loading path and grain orientation.Meanwhile,the effect of strain rate on the structural evolution of NC Mg was investigated.It is found that the effect of strain rate on the plastic deformation of NC Mg is reflected through the plasticity delays and the way to release the stress.As the strain rate decreases,the plastic deformation mechanism gradually changes from intragranular to grain boundary.Some significant potential deformation mechanisms in the loading process were studied.It is observed that{1121}twins nucleated inside the grains,and the thickening process is completed by basal〈a〉slip of the twin boundary.The strain compatibility between twins is automatically optimized with loading.Moreover,the detwinning mechanism caused by the interaction between twins and basal stacking faults is clarified.展开更多
Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here...Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here,we develop a high-performance regenerated cellulose separator with nano-cracked structures for SCs via a binary solvent of superbase-derived ionic liquid and dimethylsulfoxide(DMSO).The unique nano-cracks with an average width of 7.45 nm arise from the acceleration of cellulose molecular reassembly by DMSO-regulated hydrogen bonding,which endows the separator with high porosity(70.2%)and excellent electrolyte retention(329%).The outstanding thermal stability(273℃)and mechanical strength(70 MPa)enable the separator to maintain its structural integrity under high temperatures and external forces.With these benefits,the SC utilizing the cellulose separator enables a high specific capacitance of 93.6 F g^(−1) at 1.0 A g^(−1) and a remarkable capacitance retention of 99.5%after 10,000 cycles compared with the commercial NKK-MPF30AC and NKK-TF4030.The robust and high-wettability cellulose separator holds promise as a superior alternative to commercial separators for advanced SCs with enhanced performance and improved safety.展开更多
Volatile organic compounds(VOCs)are significant indoor and outdoor air pollutants.Thermocatalysis is one of the most common methods for their removal,capable of completely mineralizing VOCs.Nanomaterials derived by py...Volatile organic compounds(VOCs)are significant indoor and outdoor air pollutants.Thermocatalysis is one of the most common methods for their removal,capable of completely mineralizing VOCs.Nanomaterials derived by pyrolytic means,based on the high specific surface area and high tunability of metal-organic frameworks(MOFs),have received increasing attention in the field of efficient catalysis of VOCs.Lanthanide metal-organic frameworks(Ln-MOFs)have significant research value in thermocatalytic applications for VOCs by virtue of their flexible and rich coordination behaviors,Lewis acidity,excellent oxygen storage and discharge capabilities,unique electronic structure properties of lanthanide coordination metals.Herein,a comprehensive review of the recent advances in Ln-MOF-based catalysts for thermally catalyzed VOCs elaborates on the effects based on the structure,including pyrolysis strategy,metal substitution,surface modification,and composite doping.The review addresses the mechanistic issues of Ln-MOF-based catalysts in the thermocatalysis of VOCs and highlights their characteristic advantages as efficient catalysts for VOCs.Finally,this review also summarizes the key issues and challenges,provides perspectives and outlooks on the development and design of Ln-MOFbased catalysts.It serves as a reference for enhancing the performance and selecting appropriate LnMOF-based catalysts for treating VOCs.展开更多
Carbon dioxide-enhanced oil recovery(CO_(2)-EOR)and storage is recognized as an economically feasible technique if used in suitable reservoirs.The type or form and capacity of this CO_(2) sequestration technique is sy...Carbon dioxide-enhanced oil recovery(CO_(2)-EOR)and storage is recognized as an economically feasible technique if used in suitable reservoirs.The type or form and capacity of this CO_(2) sequestration technique is synergistically affected by heat,flow,stress,and chemical reactions.Aimed at addressing the technological issues in applying CO_(2)-EOR and storage in a high water-cut reservoir in Xinjiang,China,this paper proposes a thermo-hydro-mechanical-chemical coupling method during CO_(2) flooding.The potential of CO_(2) sequestration and EOR in the target reservoir is discussed in combination with the surrogate optimization method.This method works better as it considers the evolution of structural trapping,capillary trapping,solubility trapping,and mineral trapping during CO_(2) injection as well as the influence the physical field has on the sequestration capacity for different forms of CO_(2) sequestration.The main mechanisms of CO_(2) sequestration in the high water-cut reservoir is structural trapping,followed by capillary trapping.Solubility trapping and mineral trapping have less contribution to the total sequestration capacity of CO_(2).After optimization,the cumulative oil production was 2.36×10^(6)m^(3),an increase of 0.25×10^(6)m3or 11.9%compared to the pre-optimization value.The CO_(2) sequestration capacity after optimization was 1.39×10^(6)t,which is an increase of 0.23×10^(6)t compared to values obtained before optimization;this effectively increases the area affected by CO_(2) by 24.4%.Of the four trapping mechanisms,capillary trapping and structural trapping showed a high increase of 32.5%and17.28%,respectively,while solubility trapping and mineral trapping only led to an increase of 5.1%and0.43%,respectively.This research could provide theoretical support for fully utilizing the potential of CO_(2)-EOR and sequestration technology.展开更多
Background:Hepatocellular carcinoma(HCC)remains a significant global health challenge.While firstline treatments with immune checkpoint inhibitors(ICIs)have improved patient outcomes,the selection of effective second-...Background:Hepatocellular carcinoma(HCC)remains a significant global health challenge.While firstline treatments with immune checkpoint inhibitors(ICIs)have improved patient outcomes,the selection of effective second-line therapies remains unclear.This study evaluated the efficacy and safety of regorafenib as a second-line option in advanced HCC patients post-progression on ICI-based therapies.Methods:Advanced HCC patients from eight hospitals in China who received regorafenib after progression on first-line ICI therapies,alone or combined with ICIs were enrolled.Clinical data were collected,and propensity score matching(PSM)was used to ensure comparability between treatment groups.The primary endpoint was overall survival(OS).The secondary endpoints were progression-free survival(PFS),objective response rate(ORR),disease control rate(DCR),and treatment-related adverse events.The study was registered at www.chictr.org.cn(ChiCTR2400091318).Results:A total of 149 patients were included:113 in the combination therapy group(Rego-ICI group)and 36 in the regorafenib monotherapy group(Rego group).After PSM,the Rego-ICI group showed significantly improved OS[19.0 vs.11.0 months,hazard ratio(HR)=0.426,95%confidence interval(CI):0.235–0.772,P=0.005]and PFS(4.0 vs.3.0 months,HR=0.539,95%CI:0.337–0.863,P=0.010)compared to the Rego group.Differences in ORR and DCR were not statistically significant(ORR:19.4%vs.9.7%,P=0.226;DCR:64.2%vs.48.4%,P=0.139),but the Rego-ICI group showed better disease control.Regorafenib plus ICI improved both OS and PFS with no new safety signals.Conclusions:The combination of ICIs and regorafenib significantly enhances OS in advanced HCC patients post-progression on first-line ICI treatments.These findings support the potential of regorafenib plus ICIs as an effective second-line therapy.展开更多
The insufficient electrocatalytic activity and CO_(2)resistance hinder the application of cathode mate-rial for solid oxide fuel cells(SOFCs).In this study,we introduce a series of Pr-doped perovskite Bi_(0.8-x)Pr_(x)...The insufficient electrocatalytic activity and CO_(2)resistance hinder the application of cathode mate-rial for solid oxide fuel cells(SOFCs).In this study,we introduce a series of Pr-doped perovskite Bi_(0.8-x)Pr_(x)Ca_(0.2)FeO_(3-δ)(BPCF_(x),x=0,0.10,0.15,0.20)as candidate cathode materials,with a focus on its phase structure,oxygen desorption ability,catalytic activity,and electrochemical reduction kinetics.Among all the components,the Bi_(0.6)Pr_(0.2)Ca_(0.2)FeO_(3-δ)(BPCF0.20)catalyst shows impressive oxygen reduc-tion reaction(ORR)activity,with a low polarization resistance of 0.06Ωcm^(2)at 700℃and peak power density of 810 mW cm^(−2)at 800℃.Moreover,the BPCF0.20 cathode shows outstanding CO_(2)resistance in different CO_(2)concentrations(1%-10%)due to the larger average bond energy and higher relative acidity of Bi,Pr,and Fe ions.These findings demonstrate that BPCF_(x)are advanced cathode electrocatalysts for SOFCs.展开更多
Aluminum is the most abundant environmental pollutant.Recent studies suggest that aluminum exposure increases the risk of multiple diseases,including intestinal barrier dysfunction.We investigated whether Pueraria lob...Aluminum is the most abundant environmental pollutant.Recent studies suggest that aluminum exposure increases the risk of multiple diseases,including intestinal barrier dysfunction.We investigated whether Pueraria lobata extract(PLE)is effective in safeguarding against aluminum chloride exposureexacerbated intestinal barrier dysfunction.Using an experimental colitis model of aluminum-exacerbated dextran sulphate sodium(DSS)-treated mice,clinical and pathological evidence suggested that the administration of PLE counteracted aluminum exposure-induced intestinal barrier damage.In addition,we found that aluminum toxicities,including loss of tight junction molecules(TJs),upregulated pro-inflammatory cytokines,and enhanced myeloperoxidase(MPO)activity,were significantly suppressed by PLE administration.Furthermore,PLE administration was identified to inhibit activation of MAPKs and NF-κB signal pathways,which contribute to upregulation of myosin light-chain kinase(MLCK)in inflamed intestine.Taken together,these results suggest that PLE might be a potential candidate for aluminum exposure-related intestinal barrier dysfunction.展开更多
Fluorophores emitting in the second near-infrared window (NIR-II, 900–1700nm) allow for high-resolution deep-tissue bioimaging owing to minimal tissue scattering. Although J-aggregation offers a promising approach to...Fluorophores emitting in the second near-infrared window (NIR-II, 900–1700nm) allow for high-resolution deep-tissue bioimaging owing to minimal tissue scattering. Although J-aggregation offers a promising approach to developing long-wavelength emitters, the scarcity of J-type backbones and reliable design principles limits their application in biological imaging. Here, we introduce a strategy for engineering high-brightness NIR-II J-aggregated fluorophores by incorporating electron-withdrawing substituents into a fused-ring backbone. These substituents modulate the electrostatic potential (ESP) distribution across the conjugated backbone, reducing both electrostatic repulsion and intermolecular distance, which promotes ordered J-aggregation. As a result, Y8 aggregate (Y8 nanoparticles) exhibits an outstanding fluorescence quantum yield of up to 12.9% and strong near-infrared absorption in aqueous solution for high-performance NIR-II fluorescence imaging in vivo. This work not only presents a novel J-type backbone but also advances the understanding of the structure–property relationship critical to designing NIR-II J-aggregates.展开更多
[Objectives]To quantify the efficacy of Extracorporeal Shock Wave Therapy(ESWT)for upper limb spasticity in stroke patients using Modified Ashworth Scale(MAS)across three timeframes:immediate,short-term(24 h-4 wks),an...[Objectives]To quantify the efficacy of Extracorporeal Shock Wave Therapy(ESWT)for upper limb spasticity in stroke patients using Modified Ashworth Scale(MAS)across three timeframes:immediate,short-term(24 h-4 wks),and long-term(>4 wks).[Methods]Six databases(PubMed,EMBASE,CENTRAL,CINAHL,Scopus,Web of Science)until May 2025,were systematically searched,identifying 11 RCTs(n=505)meeting inclusion criteria(adults≥18 yrs old with stroke-related spasticity[MAS≥1]).Risk of bias was rigorously assessed using Cochrane criteria,revealing uniformly low risk across all domains.Random-effects meta-analyses(REML model)calculated Hedges'g with 95%CI for MAS outcomes,with subgroup analyses for joint-specific effects.[Results]ESWT demonstrated non-significant immediate MAS reduction(g=-0.69,p=0.07;=93.95%),but statistically significant short-term(g=-0.58,p=0.02;I^(2)=89.64%)and long-term effects(g=-0.52,p=0.02;I^(2)=78.72%).Effects were driven by composite MAS scores(short-term g=-0.63;long-term g=-0.56,both p<0.05)rather than isolated joints(elbow/finger/wrist all non-significant).Substantial heterogeneity persisted across analyses,particularly in joint-specific subgroups(I^(2)>90%).[Conclusions]ESWT provides statistically significant,clinically relevant reductions in global upper limb spasticity with durable efficacy beyond four weeks,supporting its use as a non-invasive adjunct therapy despite substantial heterogeneity warranting protocol standardization.展开更多
Recently,there have been few reports on the hot extrusion process of magnesium(Mg)alloy square tubes.In this work,we introduce a novel hot extrusion process called tube continuous expanding shear extrusion(t CESE)to p...Recently,there have been few reports on the hot extrusion process of magnesium(Mg)alloy square tubes.In this work,we introduce a novel hot extrusion process called tube continuous expanding shear extrusion(t CESE)to produce Mg alloy square tubes with heterogeneous structures.Compared to previously reported hot-extruded Mg alloy tubes,the tCESE samples exhibit simultaneous enhancements in both strength and ductility.The microstructure evolution during the t CESE process was analyzed in detail.It was found that the microstructure developed consecutively as a result of twinning-induced dynamic recrystallization(TDRX),discontinuous dynamic recrystallization(DDRX)and continuous dynamic recrystallization(CDRX)with different nucleation of new orientations,while static recrystallization(SRX)promoted the formation of heterogeneous structures.The strengthening mechanism in the t CESE samples was revealed by loading-unloading-reloading(LUR)tensile tests,viscoplastic self-consistent(VPSC)simulation and intermittent tensile tests.The improvement of the strength in the tCESE samples is attributed to the size refinement of the grains and the second-phases,and more importantly,to hetero-deformation induced(HDI)strengthening.The improvement of the ductility in the t CESE samples can be attributed to the significant increase in non-basal dislocations within the heterogeneous structures during tensile deformation.展开更多
基金supported by the National Natural Science Foundation of China(Nos.42072136,41972108,and 42106144)the Natural Science Foundation of Shandong Province(Nos.ZR2023MD063,ZR2020MC041,and ZR2020QD089)+1 种基金the Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Ministry of Natural Resources(No.SKDZK20230127)the Foreign visiting scholar funded by Shandong Provincial government.
文摘Biomineralization has garnered significant attention in the field of wastewater treatment due to its notable cost reduction compared to conventional methods.The reinjection water from oilfields containing an exceedingly high concentration of calcium and ferric ions will pose amajor hazard in production.However,the utilization of biomineralization for precipitating these ions has been scarcely investigated due to limited tolerance among halophiles towards such extreme conditions.In this study,free and immobilized halophiles Virgibacillus dokdonensis were used to precipitate these ions and the effects were compared,at the same time,biomineralizationmechanisms and mineral characteristicswere further explored.The results showthat bacterial concentration and carbonic anhydrase activitywere higher when additionally adding ferric ion based on calcium ion;the content of protein,polysaccharides,deoxyribonucleic acid and humic substances in the extracellular polymers also increased compared to control.Calcium ions were biomineralized into calcite and vaterite with mul-tiple morphology.Due to iron doping,the crystallinity and thermal stability of calcium carbonate decreased,the content of O-C=O,N-C=OandC-O-PO_(3) increased,the stable carbon isotope values became much more negative,andβ-sheet in minerals disappeared.Higher calcium concentrations facilitated ferric ion precipitation,while ferric ions hindered calcium precipitation.The immobilized bacteria performed better in ferric ion removal,with a precipitation ratio exceeding 90%.Free bacteria performed better in calcium removal,and the precipitation ratio reached a maximum of 56%.This research maybe provides some reference for the co-removal of calcium and ferric ions from the oilfield wastewater.
基金financially supported by the National Key Research and Development Program of China(#2022YFD1800701)the Key Research and Development Program of the Ningxia Hui Autonomous Region(#2021BEF02028)the Chinese Agricultural Research System of MOF and MARA(#CARS-37).
文摘Lumpy skin disease(LSD)is a highly contagious disease caused by lumpy skin disease virus(LSDV)in bovines.Rapid and accurate diagnosis is very important to controll it.However,current commercial detection kits need to be improved in terms of sensitivity or specificity.This study aimed to develop a novel diagnostic competitive enzyme-linked immunosorbent assay(cELISA)based on the newly identified antigen gene LSDV034.The rLSDV034 protein was identified as a potential diagnostic antigen,and it was expressed,purified,and used to immunize BALB/c mice.Using laboratory-prepared indirect ELISA(iELISA),the positive cell lines were screened,and their blocking activity was further verified by competitive ELISA(cELISA).The cell line,1H7,was chosen to produce mouse ascites,which were purified for a monoclonal antibody(mAb,5.395 mg/mL).The heavy chain type of the 1H7 mAb was identified as IgG1a,and its light chain subtype was identified as κ.Furthermore,cELISA was developed to detect bovine serum antibodies,with rLSDV034(4μg/mL)as the coating antigen and HRP-1H7 mAb(1:300)as the competitive antibody.The cutoff value of cELISA was 55%,based on 32 negative bovine serum samples.The analytical sensitivity was 1:8,and no cross-reaction was detected with bovine viral diarrhea virus(BVDV),infectious bovine rhinotracheitis virus(IBRV),Pasteurella multocida(P.multocida),or Mycoplasma bovis(M.bovis)from the serum samples.The diagnostic sensitivity and specificity of cELISA were 98.46%(95%confidence interval,CI:91.7–100)and 100%(95%CI:89.1–100),respectively,based on the analysis of 30 LSDV-infected bovine serum samples,35 GTPV-vaccinated samples,and 32 negative samples.The overall coincidence of the cELISA with the virus neutralization test(VNT)reached 98.97%(95%CI:94.4–100).Furthermore,we used cELISA to analyze 230 clinical bovine serum samples(including 59 infected and 171 vaccinated samples)and found that the serum positivity rates of the immunized samples(on d 60 postimmunization)and infected samples were 77.78%(95%CI:70.8–83.8%)and 71.19%(95%CI:57.9–82.2),respectively.These results indicate that the developed cELISA is promising for detecting serum antibodies in naturally infected or vaccinated cattle.
基金supported by National Natural Science Foundation of China(Grant No.52474029)National Natural Science Foundation for Young Scientists of China(A)(Grant No.52525403)+2 种基金National Major Science and Technology Projects under the 14th Five-Year Plan(Grant No.2024ZD1405105)Science and Technology Innovation Team Project of Xinjiang Uygur Autonomous Region(Grant No.2024TSYCTD0018)Xinjiang Uygur Autonomous Region.
文摘This study investigates the unsteady flow characteristics of shale oil reservoirs during the depletion development process,with a particular focus on production behavior following fracturing and shut-in stages.Shale reservoirs exhibit distinctive production patterns that differ from traditional oil reservoirs,as their inflow performance does not conform to the classic steady-state relationship.Instead,production is governed by unsteady-state flow behavior,and the combined effects of thewellbore and choke cause the inflowperformance curve to evolve dynamically over time.To address these challenges,this study introduces the concept of a“Dynamic IPR curve”and develops a dynamic production analysis method that integrates production time,continuity across multi-stage state fields,and the interactions between tubing flow and choke flow.This method provides a robust framework to characterize the attenuation trend of reservoir productivity and to accurately describe wellbore flow behavior.By applying the dynamic IPR approach,the study overcomes the limitations of conventional methods,which are unable to capture the temporal variations inherent in shale reservoir production.The proposed methodology offers a theoretical foundation for improved production forecasting,optimization of choke size,and analysis of wellbore tubing characteristics,thereby supporting more effective operational decision-making across different stages of shale reservoir development.
基金financially supported by Major Science and Technology Project of Yunnan Province(202302AE090022)the National Natural Science Foundation of China(21764005,32160236)+4 种基金National Key Research and Development Program of China(2022YFC2601604)Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)the Second Phase of“Double-First Class”Program Construction of Yunnan UniversityProgram for Donglu Scholars of Yunnan Universitythe Research Innovation Fund for Graduate Students of School of Chemical Science and Technology,Yunnan University。
文摘A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid(OM)was developed.Herein,copper oxide nanoparticles(CuO NPs)were prepared,which exhibit excellent enzyme-like activity(peroxidase-like and laccase-like)and electrochemical activity.CuO@3-APBA nanoparticles(CuO@3-APBA NPs)were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid(3-APBA)in CuO NPs.3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions.Then,a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme.For the first signal,CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene(TMB)to turn the solution from colorless to blue in the presence of H_(2)O_(2)(absorbance at 652 nm).For the second signal,CuO@3-APBA can catalyze the oxidation of substrates(2,4-dichlorophenol and 4-aminoantipyrine)and turn the solution from colorless to pink(absorbance at 510 nm).For the third signal,electrochemical oxidation peak of copper ion from Cu^(+)to Cu^(2+)of Cu O@3-APBA was recorded by differential pulse voltammetry,which was used to determine the OM.The sensing platform exhibited a wide linear range(0.0000316-100.000000 ng/mL)with a low detection limit(0.0105 pg/mL),as well as showed advantages,such as satisfactory reproducibility,good stability,and excellent selectivity.The assay has the potential applications for ultrasensitive detection of allergen in foods.
基金the support from the National Natural Science Foundation of China(22478429)the Special Project Fund of Taishan-Scholars(tsqn202408101)+3 种基金the Natural Science Foundation of Shandong Province(ZR2023YQ009)CNPC Innovation Found(2024DQ02-0504)Fundamental Research Funds for the Central Universities,Ocean University of China(202364004)the State Key Laboratory of Heavy Oil Processing(SKLHOP202403003)。
文摘Alcohol oxidation is a widely used green chemical reaction.The reaction process produces flammable and explosive hydrogen,so the design of the reactor must meet stringent safety requirements.Based on the limited experimental data,utilizing the traditional numerical method of computational fluid dynamics(CFD)to simulate the gas-liquid two-phase flow reactor can mitigate the risk of danger under varying working conditions.However,the calculation process is highly time-consuming.Therefore,by integrating process simulation,computational fluid dynamics,and deep learning technologies,an intelligent hybrid chemical model based on machine learning was proposed to expedite CFD calculations,enhance the prediction of flow fields,conversion rates,and concentrations inside the reactor,and offer insights for designing and optimizing the reactor for the alcohol oxidation system.The results show that the hybrid model based on the long and short-term memory neural network achieves 99.8%accuracy in conversion rate prediction and 99.9%accuracy in product concentration prediction.Through validation,the hybrid model is accelerated by about 360 times compared with instrumental analysis in conversion rate prediction and about 45 times compared with CFD calculation in concentration distribution prediction.This hybrid model can quickly predict the conversion rate and product concentration distribution in the gas-liquid two-phase flow reactor and provide a model reference for fast prediction and accurate control in the actual chemical production process.
基金Project supported by the National Key R&D Program of China (2021YFC2901500)National Natural Science Foundation of China(22208349)+1 种基金Shandong Provincial Natural Science Youth Fund (ZR2022QB244)Shandong Energy Institute Innovation Fund (SEII202107, SEII202133)。
文摘A novel hydroxy late-rich functionalized biosorbent by grafting citric acid on the decrystallized Dicranopteris dichotoma stem(DDS) was prepared.The obtained g-DDS-3 exhibits good hydrophilicity,thermal stability and superior adsorption performance for Ce^(3+).The effects of citric acid dosage,pH and initial Ce^(3+)concentration on the adsorption experiments were investigated.The maximum adsorption capacity(Langmuir model) of Ce^(3+)by g-DDS-3 is 131.0 mg/g at pH of 7.0,which surpasses that of most previously reported biomass-based materials.Adsorption kinetic results indicate that the adsorption process conforms to the pseudo-second-order model and can achieve equilibrium quickly.By analyzing the adsorption mechanism,we find that carboxyl groups are uniformly distributed on the surface of g-DDS-3 post modification,which provides abundant adsorption sites,and the adsorption is primarily driven by the ion exchange between Ce^(3+)and carboxyl groups protons.In addition,the adsorption-desorption experiments suggest that the g-DDS-3 has excellent recyclability since the adsorption capacity still remain above 90% after five cycles.These findings prove that carboxylate-rich modified D.dichotoma has great potential for application in the effective separation and recovery of Ce^(3+)from leaching tailings.
基金supports from the projects by the NSFC[51771166]the Hebei Natural Science Foundation[E2019203452,E2021203011]+3 种基金the key project of department of education of Hebei province[ZD2021107]project of the central government guiding local science and technology development[216Z1001G]Cultivation Project for Basic Research and Innovation of Yanshan University[2021LGZD002]project of State Key Laboratory of Materials Processing and Die&Mould Technology[P2023-004]are gratefully acknowledged.
文摘Molecular dynamics(MD)simulation is employed to investigate the deformation behavior under various loading paths and strain rates of nanocrystalline magnesium(NC Mg)with[0001]texture.Atomic-scale structural evolution of NC Mg was performed under uniaxial and biaxial loadings.In tension process,compression twins and basal slip dominate,while the compression process is dominated by tension twins.The activation mechanism of twinning is highly sensitive to the loading path and grain orientation.Meanwhile,the effect of strain rate on the structural evolution of NC Mg was investigated.It is found that the effect of strain rate on the plastic deformation of NC Mg is reflected through the plasticity delays and the way to release the stress.As the strain rate decreases,the plastic deformation mechanism gradually changes from intragranular to grain boundary.Some significant potential deformation mechanisms in the loading process were studied.It is observed that{1121}twins nucleated inside the grains,and the thickening process is completed by basal〈a〉slip of the twin boundary.The strain compatibility between twins is automatically optimized with loading.Moreover,the detwinning mechanism caused by the interaction between twins and basal stacking faults is clarified.
基金supported by the National Natural Science Foundation of China(No.U22A20422,22178028)the Program of Introducing Talents of Discipline to Universities(Project 111,B21022)the 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University(No.BLRC2023B01)。
文摘Separators in supercapacitors(SCs)frequently suffer from high resistance and the risk of short circuits due to inadequate electrolyte wettability,depressed mechanical properties,and insufficient thermal stability.Here,we develop a high-performance regenerated cellulose separator with nano-cracked structures for SCs via a binary solvent of superbase-derived ionic liquid and dimethylsulfoxide(DMSO).The unique nano-cracks with an average width of 7.45 nm arise from the acceleration of cellulose molecular reassembly by DMSO-regulated hydrogen bonding,which endows the separator with high porosity(70.2%)and excellent electrolyte retention(329%).The outstanding thermal stability(273℃)and mechanical strength(70 MPa)enable the separator to maintain its structural integrity under high temperatures and external forces.With these benefits,the SC utilizing the cellulose separator enables a high specific capacitance of 93.6 F g^(−1) at 1.0 A g^(−1) and a remarkable capacitance retention of 99.5%after 10,000 cycles compared with the commercial NKK-MPF30AC and NKK-TF4030.The robust and high-wettability cellulose separator holds promise as a superior alternative to commercial separators for advanced SCs with enhanced performance and improved safety.
基金Project supported by the Science and Technology Projects of Fujian Province(2022Y0041)。
文摘Volatile organic compounds(VOCs)are significant indoor and outdoor air pollutants.Thermocatalysis is one of the most common methods for their removal,capable of completely mineralizing VOCs.Nanomaterials derived by pyrolytic means,based on the high specific surface area and high tunability of metal-organic frameworks(MOFs),have received increasing attention in the field of efficient catalysis of VOCs.Lanthanide metal-organic frameworks(Ln-MOFs)have significant research value in thermocatalytic applications for VOCs by virtue of their flexible and rich coordination behaviors,Lewis acidity,excellent oxygen storage and discharge capabilities,unique electronic structure properties of lanthanide coordination metals.Herein,a comprehensive review of the recent advances in Ln-MOF-based catalysts for thermally catalyzed VOCs elaborates on the effects based on the structure,including pyrolysis strategy,metal substitution,surface modification,and composite doping.The review addresses the mechanistic issues of Ln-MOF-based catalysts in the thermocatalysis of VOCs and highlights their characteristic advantages as efficient catalysts for VOCs.Finally,this review also summarizes the key issues and challenges,provides perspectives and outlooks on the development and design of Ln-MOFbased catalysts.It serves as a reference for enhancing the performance and selecting appropriate LnMOF-based catalysts for treating VOCs.
文摘Carbon dioxide-enhanced oil recovery(CO_(2)-EOR)and storage is recognized as an economically feasible technique if used in suitable reservoirs.The type or form and capacity of this CO_(2) sequestration technique is synergistically affected by heat,flow,stress,and chemical reactions.Aimed at addressing the technological issues in applying CO_(2)-EOR and storage in a high water-cut reservoir in Xinjiang,China,this paper proposes a thermo-hydro-mechanical-chemical coupling method during CO_(2) flooding.The potential of CO_(2) sequestration and EOR in the target reservoir is discussed in combination with the surrogate optimization method.This method works better as it considers the evolution of structural trapping,capillary trapping,solubility trapping,and mineral trapping during CO_(2) injection as well as the influence the physical field has on the sequestration capacity for different forms of CO_(2) sequestration.The main mechanisms of CO_(2) sequestration in the high water-cut reservoir is structural trapping,followed by capillary trapping.Solubility trapping and mineral trapping have less contribution to the total sequestration capacity of CO_(2).After optimization,the cumulative oil production was 2.36×10^(6)m^(3),an increase of 0.25×10^(6)m3or 11.9%compared to the pre-optimization value.The CO_(2) sequestration capacity after optimization was 1.39×10^(6)t,which is an increase of 0.23×10^(6)t compared to values obtained before optimization;this effectively increases the area affected by CO_(2) by 24.4%.Of the four trapping mechanisms,capillary trapping and structural trapping showed a high increase of 32.5%and17.28%,respectively,while solubility trapping and mineral trapping only led to an increase of 5.1%and0.43%,respectively.This research could provide theoretical support for fully utilizing the potential of CO_(2)-EOR and sequestration technology.
基金supported by a grant from the Zhongda Hospital Affiliated to Southeast University,Jiangsu Province High-Level Hospital Construction Funds(No.YKK24270)。
文摘Background:Hepatocellular carcinoma(HCC)remains a significant global health challenge.While firstline treatments with immune checkpoint inhibitors(ICIs)have improved patient outcomes,the selection of effective second-line therapies remains unclear.This study evaluated the efficacy and safety of regorafenib as a second-line option in advanced HCC patients post-progression on ICI-based therapies.Methods:Advanced HCC patients from eight hospitals in China who received regorafenib after progression on first-line ICI therapies,alone or combined with ICIs were enrolled.Clinical data were collected,and propensity score matching(PSM)was used to ensure comparability between treatment groups.The primary endpoint was overall survival(OS).The secondary endpoints were progression-free survival(PFS),objective response rate(ORR),disease control rate(DCR),and treatment-related adverse events.The study was registered at www.chictr.org.cn(ChiCTR2400091318).Results:A total of 149 patients were included:113 in the combination therapy group(Rego-ICI group)and 36 in the regorafenib monotherapy group(Rego group).After PSM,the Rego-ICI group showed significantly improved OS[19.0 vs.11.0 months,hazard ratio(HR)=0.426,95%confidence interval(CI):0.235–0.772,P=0.005]and PFS(4.0 vs.3.0 months,HR=0.539,95%CI:0.337–0.863,P=0.010)compared to the Rego group.Differences in ORR and DCR were not statistically significant(ORR:19.4%vs.9.7%,P=0.226;DCR:64.2%vs.48.4%,P=0.139),but the Rego-ICI group showed better disease control.Regorafenib plus ICI improved both OS and PFS with no new safety signals.Conclusions:The combination of ICIs and regorafenib significantly enhances OS in advanced HCC patients post-progression on first-line ICI treatments.These findings support the potential of regorafenib plus ICIs as an effective second-line therapy.
基金supported by the Natural Science Foundation of Heilongjiang Province(No.ZD2022E007).
文摘The insufficient electrocatalytic activity and CO_(2)resistance hinder the application of cathode mate-rial for solid oxide fuel cells(SOFCs).In this study,we introduce a series of Pr-doped perovskite Bi_(0.8-x)Pr_(x)Ca_(0.2)FeO_(3-δ)(BPCF_(x),x=0,0.10,0.15,0.20)as candidate cathode materials,with a focus on its phase structure,oxygen desorption ability,catalytic activity,and electrochemical reduction kinetics.Among all the components,the Bi_(0.6)Pr_(0.2)Ca_(0.2)FeO_(3-δ)(BPCF0.20)catalyst shows impressive oxygen reduc-tion reaction(ORR)activity,with a low polarization resistance of 0.06Ωcm^(2)at 700℃and peak power density of 810 mW cm^(−2)at 800℃.Moreover,the BPCF0.20 cathode shows outstanding CO_(2)resistance in different CO_(2)concentrations(1%-10%)due to the larger average bond energy and higher relative acidity of Bi,Pr,and Fe ions.These findings demonstrate that BPCF_(x)are advanced cathode electrocatalysts for SOFCs.
基金supported by the Shangrao Key Research and Development Project(19A005)。
文摘Aluminum is the most abundant environmental pollutant.Recent studies suggest that aluminum exposure increases the risk of multiple diseases,including intestinal barrier dysfunction.We investigated whether Pueraria lobata extract(PLE)is effective in safeguarding against aluminum chloride exposureexacerbated intestinal barrier dysfunction.Using an experimental colitis model of aluminum-exacerbated dextran sulphate sodium(DSS)-treated mice,clinical and pathological evidence suggested that the administration of PLE counteracted aluminum exposure-induced intestinal barrier damage.In addition,we found that aluminum toxicities,including loss of tight junction molecules(TJs),upregulated pro-inflammatory cytokines,and enhanced myeloperoxidase(MPO)activity,were significantly suppressed by PLE administration.Furthermore,PLE administration was identified to inhibit activation of MAPKs and NF-κB signal pathways,which contribute to upregulation of myosin light-chain kinase(MLCK)in inflamed intestine.Taken together,these results suggest that PLE might be a potential candidate for aluminum exposure-related intestinal barrier dysfunction.
基金support from the National Natural Science Foundation of China (Nos. 62175201 and 52373142)the Natural Science Foundation of Jiangsu Province of China (No. BK20220404)+1 种基金the Fundamental Research Funds for the Central Universitiesthe open research fund of State Key Laboratory of Organic Electronics and Information Displays.
文摘Fluorophores emitting in the second near-infrared window (NIR-II, 900–1700nm) allow for high-resolution deep-tissue bioimaging owing to minimal tissue scattering. Although J-aggregation offers a promising approach to developing long-wavelength emitters, the scarcity of J-type backbones and reliable design principles limits their application in biological imaging. Here, we introduce a strategy for engineering high-brightness NIR-II J-aggregated fluorophores by incorporating electron-withdrawing substituents into a fused-ring backbone. These substituents modulate the electrostatic potential (ESP) distribution across the conjugated backbone, reducing both electrostatic repulsion and intermolecular distance, which promotes ordered J-aggregation. As a result, Y8 aggregate (Y8 nanoparticles) exhibits an outstanding fluorescence quantum yield of up to 12.9% and strong near-infrared absorption in aqueous solution for high-performance NIR-II fluorescence imaging in vivo. This work not only presents a novel J-type backbone but also advances the understanding of the structure–property relationship critical to designing NIR-II J-aggregates.
文摘[Objectives]To quantify the efficacy of Extracorporeal Shock Wave Therapy(ESWT)for upper limb spasticity in stroke patients using Modified Ashworth Scale(MAS)across three timeframes:immediate,short-term(24 h-4 wks),and long-term(>4 wks).[Methods]Six databases(PubMed,EMBASE,CENTRAL,CINAHL,Scopus,Web of Science)until May 2025,were systematically searched,identifying 11 RCTs(n=505)meeting inclusion criteria(adults≥18 yrs old with stroke-related spasticity[MAS≥1]).Risk of bias was rigorously assessed using Cochrane criteria,revealing uniformly low risk across all domains.Random-effects meta-analyses(REML model)calculated Hedges'g with 95%CI for MAS outcomes,with subgroup analyses for joint-specific effects.[Results]ESWT demonstrated non-significant immediate MAS reduction(g=-0.69,p=0.07;=93.95%),but statistically significant short-term(g=-0.58,p=0.02;I^(2)=89.64%)and long-term effects(g=-0.52,p=0.02;I^(2)=78.72%).Effects were driven by composite MAS scores(short-term g=-0.63;long-term g=-0.56,both p<0.05)rather than isolated joints(elbow/finger/wrist all non-significant).Substantial heterogeneity persisted across analyses,particularly in joint-specific subgroups(I^(2)>90%).[Conclusions]ESWT provides statistically significant,clinically relevant reductions in global upper limb spasticity with durable efficacy beyond four weeks,supporting its use as a non-invasive adjunct therapy despite substantial heterogeneity warranting protocol standardization.
基金the National Science Foundation of China(52071042)Chongqing Talent Plan(CQYC202003047)+1 种基金the Chongqing Natural Science Foundation Project of CSTB2021YCJH-BGZXM0148 and CSTB2023NSCQ-MSX0079the Open Fund of the National Key Laboratory of Advanced Design and Manufacturing Technology for Vehicle,Hunan University(Grant No.82315001)。
文摘Recently,there have been few reports on the hot extrusion process of magnesium(Mg)alloy square tubes.In this work,we introduce a novel hot extrusion process called tube continuous expanding shear extrusion(t CESE)to produce Mg alloy square tubes with heterogeneous structures.Compared to previously reported hot-extruded Mg alloy tubes,the tCESE samples exhibit simultaneous enhancements in both strength and ductility.The microstructure evolution during the t CESE process was analyzed in detail.It was found that the microstructure developed consecutively as a result of twinning-induced dynamic recrystallization(TDRX),discontinuous dynamic recrystallization(DDRX)and continuous dynamic recrystallization(CDRX)with different nucleation of new orientations,while static recrystallization(SRX)promoted the formation of heterogeneous structures.The strengthening mechanism in the t CESE samples was revealed by loading-unloading-reloading(LUR)tensile tests,viscoplastic self-consistent(VPSC)simulation and intermittent tensile tests.The improvement of the strength in the tCESE samples is attributed to the size refinement of the grains and the second-phases,and more importantly,to hetero-deformation induced(HDI)strengthening.The improvement of the ductility in the t CESE samples can be attributed to the significant increase in non-basal dislocations within the heterogeneous structures during tensile deformation.
