BACKGROUND Radiation enteritis is a common complication of radiation therapy in which the surrounding normal intestinal tissue is damaged by ionising radiation,and there is no standard pharmacological prophylaxis or t...BACKGROUND Radiation enteritis is a common complication of radiation therapy in which the surrounding normal intestinal tissue is damaged by ionising radiation,and there is no standard pharmacological prophylaxis or treatment regimen available.Mesenchymal stem cell transplantation can be used for radiation protection and the treatment of acute radiation injury,but its therapeutic mechanism of action remains unclear.AIM To investigate the protective effects of autologous bone marrow-derived mesenchymal stem cell(ABMSC)transplantation on radiation-induced intestinal injury.METHODS A model of acute radioactive enteritis was established in dogs by applying abdominal intensity-modulated radiation at a single X-ray dose of 12 Gy.ABMSCs were transplanted into the mesenteric artery with the technology of femoral artery puncture and DSA imaging two days after radiation.Visual and histopathological changes of the experimental dogs were observed.Different kinds of cytokines from intestinal samples were tested using Quantibody Canine Cytokine Array method.Enzyme-linked immunosorbent assay(ELISA)was also used to evaluate the cytokines changes in serum.RESULTS The ABMSCs group showed significant improvements in survival status compared with the blank and saline treatment groups.Histological observations revealed that the former had lower histological scores than the later after treatment(P<0.05).Compared to the control groups,interleukin(IL)-10 and monocyte chemotactic protein(MCP)-1 from intestinal samples showed a remarkable increase and ELISA of serum samples proved higher secretion of the two target cytokines in the ABMSCs group(P<0.05).CONCLUSION Our data suggest that transplantation of ABMSCs promotes intestinal recovery after acute radioactive injury in Beagle dogs.The cytokines of IL-10 and MCP-1 might play an important role in this process.展开更多
Background:Primary liver cancer poses a significant global health burden,with projections indicating a surpassing of one million cases by 2025.Cuproptosis,a copper-dependent mechanism of cell death,plays a crucial rol...Background:Primary liver cancer poses a significant global health burden,with projections indicating a surpassing of one million cases by 2025.Cuproptosis,a copper-dependent mechanism of cell death,plays a crucial role in the pathogenesis,progression,and prognosis of various cancers,including hepatocellular carcinoma(HCC).Purpose:This study aimed to develop a prognostic model for HCC based on cuproptosis-related genes,utilizing clinical data and gene expression profiles from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases.Materials and Methods:Clinical features and gene expression data of HCC patients were collected from publicly available databases.Patients from TCGA were randomly divided into training and testing sets,and Lasso Cox regression was applied to develop a predictive model using cuproptosis-related genes.Results:The analysis identified Copper Metabolism Domain Containing 1(COMMD1)as a potential prognostic marker for HCC,with deletion of this gene impacting disease progression.Cellular functional experiments validated the role of COMMD1 in HCC.Conclusions:COMMD1 emerges as a promising candidate for HCC treatment,with implications for prognosis prediction and therapeutic targeting.展开更多
Objective:To design a disposable care package for postoperative PICC disinfection for the daily maintenance of postoperative indwelling PICC catheter patients,save the operation time of nurses,reduce the operation dif...Objective:To design a disposable care package for postoperative PICC disinfection for the daily maintenance of postoperative indwelling PICC catheter patients,save the operation time of nurses,reduce the operation difficulty of nurses,increase patient comfort and improve patient satisfaction.Methods:The items required for the routine maintenance of PICC catheter shall be packaged separately in a sterile manner,and all items shall be packaged as disposable care package for the maintenance of PICC catheter.Results:The use of a special PICC disinfection disposable care package,inside all the items after strict sterile treatment,simple and convenient,save time and effort.Conclusion:PICC disinfection disposable care package can reduce the operation difficulty of medical staff,shorten the operation time,increase patient comfort,and facilitate the use of medical staff.展开更多
DNA barcoding has been widely used for herb identification in recent decades,enabling safety and innovation in the field of herbal medicine.In this article,we summarize recent progress in DNA bar-coding for herbal med...DNA barcoding has been widely used for herb identification in recent decades,enabling safety and innovation in the field of herbal medicine.In this article,we summarize recent progress in DNA bar-coding for herbal medicine to provide ideas for the further development and application of this tech-nology.Most importantly,the standard DNA barcode has been extended in two ways.First,while conventional DNA barcodes have been widely promoted for their versatility in the identification of fresh or well-preserved samples,super-barcodes based on plastid genomes have rapidly developed and have shown advantages in species identification at low taxonomic levels.Second,mini-barcodes are attractive because they perform better in cases of degraded DNA from herbal materials.In addition,some mo-lecular techniques,such as high-throughput sequencing and isothermal amplification,are combined with DNA barcodes for species identification,which has expanded the applications of herb identification based on DNA barcoding and brought about the post-DNA-barcoding era.Furthermore,standard and high-species coverage DNA barcode reference libraries have been constructed to provide reference se-quences for species identification,which increases the accuracy and credibility of species discrimination based on DNA barcodes.In summary,DNA barcoding should play a key role in the quality control of traditional herbal medicine and in the international herb trade.展开更多
AIM To assess the value of combined acoustic radiation force impulse(ARFI) imaging, serological indexes and contrast-enhanced ultrasound(CEUS) in distinguishing between benign and malignant liver lesions. METHODS Pati...AIM To assess the value of combined acoustic radiation force impulse(ARFI) imaging, serological indexes and contrast-enhanced ultrasound(CEUS) in distinguishing between benign and malignant liver lesions. METHODS Patients with liver lesions treated at our hospital were included in this study. The lesions were divided into either a malignant tumor group or a benign tumor group according to pathological or radiological findings. ARFI quantitative detection, serological testing and CEUS quantitative detection were performed and compared. A comparative analysis of the measured indexes was performed between these groups. Receiver operating characteristic(ROC) curves were constructed to compare the diagnostic accuracy of ARFI imaging, serological indexes and CEUS, alone or in different combinations, in identifying benign and malignant liver lesions. RESULTS A total of 112 liver lesions in 43 patients were included, of which 78 were malignant and 34 were benign. Shear wave velocity(SWV) value, serum alpha-fetoprotein(AFP) content and enhancement rate were significantly higher in the malignant tumor group than in the benign tumor group(2.39 ± 1.20 m/s vs 1.50 ± 0.49 m/s, 18.02 ± 5.01 ng/m L vs 15.96 ± 4.33 ng/m L, 2.14 ± 0.21 d B/s vs 2.01 ± 0.31 d B/s; P < 0.05). The ROC curve analysis revealed that the areas under the curves(AUCs) of SWV value alone, AFP content alone, enhancement rate alone, SWV value + AFP content, SWV value + enhancement rate, AFP content + enhancement rate and SWV value + AFP content + enhancement rate were 85.1%, 72.1%, 74.5%, 88.3%, 90.4%, 82.0% and 92.3%, respectively. The AUC of SWV value + AFP content + enhancement rate was higher than those of SWV value + AFP content and SWV value + enhancement rate, and significantly higher than those of any single parameter or the combination of any two of parameters.CONCLUSION The combination of SWV, AFP and enhancement rate had better diagnostic performance in distinguishing between benign and malignant liver lesions than the use of any single parameter or the combination of any two of parameters. It is expected that this would provide a tool for the differential diagnosis of benign and malignant liver lesions.展开更多
AIM:To evaluate the correlations between cytotoxic T lymphocyte-associated antigen-4(CTLA-4) and multidrug resistance 1(MDR1) genes polymorphisms with ulcerative colitis(UC) risk.METHODS:Pub Med,EMBASE,Web of Science,...AIM:To evaluate the correlations between cytotoxic T lymphocyte-associated antigen-4(CTLA-4) and multidrug resistance 1(MDR1) genes polymorphisms with ulcerative colitis(UC) risk.METHODS:Pub Med,EMBASE,Web of Science,Cochrane Library,CBM databases,Springerlink,Wiley,EBSCO,Ovid,Wanfang database,VIP database,China National Knowledge Infrastructure,and Weipu Journal databases were exhaustively searched using combinations of keywords relating to CTLA-4,MDR1 and UC. The published studies were filtered using our stringent inclusion and exclusion criteria,the quality assessment for each eligible study was conducted using Critical Appraisal Skill Program and the resultant high-quality data from final selected studies were analyzed using Comprehensive Meta-analysis 2.0(CMA 2.0) software. The correlations between SNPs of CTLA-4 gene,MDR1 gene and the risk of UC were evaluated by OR at 95%CI. Z test was carried out to evaluate the significance of overall effect values. Cochran's Q-statistic and I2 tests were applied to quantify heterogeneity among studies. Funnel plots,classic fail-safe N and Egger's linear regression test were inspected for indication of publication bias.RESULTS:A total of 107 studies were initially retrieved and 12 studies were eventually selected for metaanalysis. These 12 case-control studies involved 1860 UC patients and 2663 healthy controls. Our major result revealed that single nucleotide polymorphisms(SNPs) of CTLA-4 gene rs3087243 G > A and rs231775 G > A may increase the risk of UC(rs3087243 G > A:allele model:OR = 1.365,95%CI:1.023-1.822,P = 0.035; dominant model:OR = 1.569,95%CI:1.269-1.940,P < 0.001; rs231775 G > A:allele model:OR = 1.583,95%CI:= 1.306-1.918,P < 0.001; dominant model:OR = 1.805,95%CI:1.393-2.340,P < 0.001). In addition,based on our result,SNPs of MDR1 gene rs1045642 C > T might also confer a significant increases for the risk of UC(allele model:OR = 1.389,95%CI:1.214-1.590,P < 0.001; dominant model:OR = 1.518,95%CI:1.222-1.886,P < 0.001).CONCLUSION:CTLA-4 gene rs3087243 G > A and rs231775 G > A,and MDR1 gene rs1045642 C > T might confer an increase for UC risk.展开更多
Relaxed open-mouth display serves important social functions in relation to submission, reconciliation affiliation and reassurance among non-human primate societies; however, quantitative evidence on this behavior rem...Relaxed open-mouth display serves important social functions in relation to submission, reconciliation affiliation and reassurance among non-human primate societies; however, quantitative evidence on this behavior remains insufficient among multi-level social groups. From July to November 2016, we examined four potential functions of the relaxed open-mouth display during pairwise, intra-unit social interactions among 18 free-ranging adult and sub-adult golden snub-nosed monkeys(Rhinopithecus roxellana) who belonged to three one-male, multi-female units(OMU) at Dalongtan, Shennongjia National Park China. Results showed that: compared with no relaxed open-mouth display,(1) the occurrence of displacement by a dominant individual approaching a subordinate was lower and the distance of the subordinate to the approaching dominant was shorter when the subordinate showed open-mouth display;(2) relaxed open-mouth display reduced the probability of continued attack for victims of aggression and allowed victims to achieve closer proximity to the aggressor during post-conflict periods;(3) relaxed open-mouth display by dominant individuals allowed them to achieve closer proximity to subordinates; and(4) the exchange of relaxed open-mouth display had a greater impact on the outcome of interactions than one individual alone giving this signal. These findings suggest that relaxed open-mouth display serves important functions regarding submission, reconciliation, affiliation and reassurance in coordinating social interactions within OMUs in golden snub-nosed monkeys.展开更多
Numerous plants and animals are edible and officinal,but some can be poisonous.There is sometimes confusion between poisonous and non-poisonous materials because of similarities in their morphologies.Consequently,the ...Numerous plants and animals are edible and officinal,but some can be poisonous.There is sometimes confusion between poisonous and non-poisonous materials because of similarities in their morphologies.Consequently,the unwitting intake of poisonous plant or animal material has resulted in poisoning cases and sometimes in death,especially for situation in the wild.Rapid and accurate authentication of toxic species is essential for establishing and adopting optimal and urgent treatment for patients in such cases,and can be life-saving or can at least minimize the damage to health.Unfortunately,most of the current species authentication methods,including DNA barcoding,loop-mediated isothermal amplification(LAMP),chromatography technologies,and other methods,depend on professional equipment and a specialist laboratory,which are impracticable for real-time application in the field.It is therefore crucial to develop a rapid,accurate,and specific authentication method for poisonous species that does not require any equipment.展开更多
Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process,and ovarian aging is shown by a decrease in the number and quality of oocytes.However,little is known a...Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process,and ovarian aging is shown by a decrease in the number and quality of oocytes.However,little is known about the molecular mechanisms of female age-related fertility decline in different types of ovarian cells during aging,especially in goats.Therefore,the aim of this study was to reveal the mechanisms driving ovarian aging in goats at single-cell resolution.Results For the first time,we surveyed the single-cell transcriptomic landscape of over 27,000 ovarian cells from newborn,young and aging goats,and identified nine ovarian cell types with distinct gene-expression signatures.Functional enrichment analysis showed that ovarian cell types were involved in their own unique biological processes,such as Wnt beta-catenin signalling was enriched in germ cells,whereas ovarian steroidogenesis was enriched in granulosa cells(GCs).Further analysis showed that ovarian aging was linked to GCs-specific changes in the antioxidant system,oxidative phosphorylation,and apoptosis.Subsequently,we identified a series of dynamic genes,such as AMH,CRABP2,THBS1 and TIMP1,which determined the fate of GCs.Additionally,FOXO1,SOX4,and HIF1A were identified as significant regulons that instructed the differentiation of GCs in a distinct manner during ovarian aging.Conclusions This study revealed a comprehensive aging-associated transcriptomic atlas characterizing the cell typespecific mechanisms during ovarian aging at the single-cell level and offers new diagnostic biomarkers and potential therapeutic targets for age-related goat ovarian diseases.展开更多
基金Supported by People's Livelihood Science and Technology Plan Joint Project of Liaoning Province,No.2021JH2/10300083。
文摘BACKGROUND Radiation enteritis is a common complication of radiation therapy in which the surrounding normal intestinal tissue is damaged by ionising radiation,and there is no standard pharmacological prophylaxis or treatment regimen available.Mesenchymal stem cell transplantation can be used for radiation protection and the treatment of acute radiation injury,but its therapeutic mechanism of action remains unclear.AIM To investigate the protective effects of autologous bone marrow-derived mesenchymal stem cell(ABMSC)transplantation on radiation-induced intestinal injury.METHODS A model of acute radioactive enteritis was established in dogs by applying abdominal intensity-modulated radiation at a single X-ray dose of 12 Gy.ABMSCs were transplanted into the mesenteric artery with the technology of femoral artery puncture and DSA imaging two days after radiation.Visual and histopathological changes of the experimental dogs were observed.Different kinds of cytokines from intestinal samples were tested using Quantibody Canine Cytokine Array method.Enzyme-linked immunosorbent assay(ELISA)was also used to evaluate the cytokines changes in serum.RESULTS The ABMSCs group showed significant improvements in survival status compared with the blank and saline treatment groups.Histological observations revealed that the former had lower histological scores than the later after treatment(P<0.05).Compared to the control groups,interleukin(IL)-10 and monocyte chemotactic protein(MCP)-1 from intestinal samples showed a remarkable increase and ELISA of serum samples proved higher secretion of the two target cytokines in the ABMSCs group(P<0.05).CONCLUSION Our data suggest that transplantation of ABMSCs promotes intestinal recovery after acute radioactive injury in Beagle dogs.The cytokines of IL-10 and MCP-1 might play an important role in this process.
文摘Background:Primary liver cancer poses a significant global health burden,with projections indicating a surpassing of one million cases by 2025.Cuproptosis,a copper-dependent mechanism of cell death,plays a crucial role in the pathogenesis,progression,and prognosis of various cancers,including hepatocellular carcinoma(HCC).Purpose:This study aimed to develop a prognostic model for HCC based on cuproptosis-related genes,utilizing clinical data and gene expression profiles from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases.Materials and Methods:Clinical features and gene expression data of HCC patients were collected from publicly available databases.Patients from TCGA were randomly divided into training and testing sets,and Lasso Cox regression was applied to develop a predictive model using cuproptosis-related genes.Results:The analysis identified Copper Metabolism Domain Containing 1(COMMD1)as a potential prognostic marker for HCC,with deletion of this gene impacting disease progression.Cellular functional experiments validated the role of COMMD1 in HCC.Conclusions:COMMD1 emerges as a promising candidate for HCC treatment,with implications for prognosis prediction and therapeutic targeting.
文摘Objective:To design a disposable care package for postoperative PICC disinfection for the daily maintenance of postoperative indwelling PICC catheter patients,save the operation time of nurses,reduce the operation difficulty of nurses,increase patient comfort and improve patient satisfaction.Methods:The items required for the routine maintenance of PICC catheter shall be packaged separately in a sterile manner,and all items shall be packaged as disposable care package for the maintenance of PICC catheter.Results:The use of a special PICC disinfection disposable care package,inside all the items after strict sterile treatment,simple and convenient,save time and effort.Conclusion:PICC disinfection disposable care package can reduce the operation difficulty of medical staff,shorten the operation time,increase patient comfort,and facilitate the use of medical staff.
基金funded by the National Natural Science Foundation of China(Grant No.:U1812403-1)the China Academy of Chinese Medical Sciences Innovation Fund(Grant No.:CI2021A03910).
文摘DNA barcoding has been widely used for herb identification in recent decades,enabling safety and innovation in the field of herbal medicine.In this article,we summarize recent progress in DNA bar-coding for herbal medicine to provide ideas for the further development and application of this tech-nology.Most importantly,the standard DNA barcode has been extended in two ways.First,while conventional DNA barcodes have been widely promoted for their versatility in the identification of fresh or well-preserved samples,super-barcodes based on plastid genomes have rapidly developed and have shown advantages in species identification at low taxonomic levels.Second,mini-barcodes are attractive because they perform better in cases of degraded DNA from herbal materials.In addition,some mo-lecular techniques,such as high-throughput sequencing and isothermal amplification,are combined with DNA barcodes for species identification,which has expanded the applications of herb identification based on DNA barcoding and brought about the post-DNA-barcoding era.Furthermore,standard and high-species coverage DNA barcode reference libraries have been constructed to provide reference se-quences for species identification,which increases the accuracy and credibility of species discrimination based on DNA barcodes.In summary,DNA barcoding should play a key role in the quality control of traditional herbal medicine and in the international herb trade.
基金Supported by Yangpu District Health and Family Planning Commission,Yangpu District Science and Technology Commission,No.YP15M18Research Project of Shanghai Municipal Commission of Health and Family Planning,No.201540032
文摘AIM To assess the value of combined acoustic radiation force impulse(ARFI) imaging, serological indexes and contrast-enhanced ultrasound(CEUS) in distinguishing between benign and malignant liver lesions. METHODS Patients with liver lesions treated at our hospital were included in this study. The lesions were divided into either a malignant tumor group or a benign tumor group according to pathological or radiological findings. ARFI quantitative detection, serological testing and CEUS quantitative detection were performed and compared. A comparative analysis of the measured indexes was performed between these groups. Receiver operating characteristic(ROC) curves were constructed to compare the diagnostic accuracy of ARFI imaging, serological indexes and CEUS, alone or in different combinations, in identifying benign and malignant liver lesions. RESULTS A total of 112 liver lesions in 43 patients were included, of which 78 were malignant and 34 were benign. Shear wave velocity(SWV) value, serum alpha-fetoprotein(AFP) content and enhancement rate were significantly higher in the malignant tumor group than in the benign tumor group(2.39 ± 1.20 m/s vs 1.50 ± 0.49 m/s, 18.02 ± 5.01 ng/m L vs 15.96 ± 4.33 ng/m L, 2.14 ± 0.21 d B/s vs 2.01 ± 0.31 d B/s; P < 0.05). The ROC curve analysis revealed that the areas under the curves(AUCs) of SWV value alone, AFP content alone, enhancement rate alone, SWV value + AFP content, SWV value + enhancement rate, AFP content + enhancement rate and SWV value + AFP content + enhancement rate were 85.1%, 72.1%, 74.5%, 88.3%, 90.4%, 82.0% and 92.3%, respectively. The AUC of SWV value + AFP content + enhancement rate was higher than those of SWV value + AFP content and SWV value + enhancement rate, and significantly higher than those of any single parameter or the combination of any two of parameters.CONCLUSION The combination of SWV, AFP and enhancement rate had better diagnostic performance in distinguishing between benign and malignant liver lesions than the use of any single parameter or the combination of any two of parameters. It is expected that this would provide a tool for the differential diagnosis of benign and malignant liver lesions.
文摘AIM:To evaluate the correlations between cytotoxic T lymphocyte-associated antigen-4(CTLA-4) and multidrug resistance 1(MDR1) genes polymorphisms with ulcerative colitis(UC) risk.METHODS:Pub Med,EMBASE,Web of Science,Cochrane Library,CBM databases,Springerlink,Wiley,EBSCO,Ovid,Wanfang database,VIP database,China National Knowledge Infrastructure,and Weipu Journal databases were exhaustively searched using combinations of keywords relating to CTLA-4,MDR1 and UC. The published studies were filtered using our stringent inclusion and exclusion criteria,the quality assessment for each eligible study was conducted using Critical Appraisal Skill Program and the resultant high-quality data from final selected studies were analyzed using Comprehensive Meta-analysis 2.0(CMA 2.0) software. The correlations between SNPs of CTLA-4 gene,MDR1 gene and the risk of UC were evaluated by OR at 95%CI. Z test was carried out to evaluate the significance of overall effect values. Cochran's Q-statistic and I2 tests were applied to quantify heterogeneity among studies. Funnel plots,classic fail-safe N and Egger's linear regression test were inspected for indication of publication bias.RESULTS:A total of 107 studies were initially retrieved and 12 studies were eventually selected for metaanalysis. These 12 case-control studies involved 1860 UC patients and 2663 healthy controls. Our major result revealed that single nucleotide polymorphisms(SNPs) of CTLA-4 gene rs3087243 G > A and rs231775 G > A may increase the risk of UC(rs3087243 G > A:allele model:OR = 1.365,95%CI:1.023-1.822,P = 0.035; dominant model:OR = 1.569,95%CI:1.269-1.940,P < 0.001; rs231775 G > A:allele model:OR = 1.583,95%CI:= 1.306-1.918,P < 0.001; dominant model:OR = 1.805,95%CI:1.393-2.340,P < 0.001). In addition,based on our result,SNPs of MDR1 gene rs1045642 C > T might also confer a significant increases for the risk of UC(allele model:OR = 1.389,95%CI:1.214-1.590,P < 0.001; dominant model:OR = 1.518,95%CI:1.222-1.886,P < 0.001).CONCLUSION:CTLA-4 gene rs3087243 G > A and rs231775 G > A,and MDR1 gene rs1045642 C > T might confer an increase for UC risk.
基金supported by the National Natural Science Foundation of China(31670397,31870509)Science Foundation of the State Forestry Administration of China
文摘Relaxed open-mouth display serves important social functions in relation to submission, reconciliation affiliation and reassurance among non-human primate societies; however, quantitative evidence on this behavior remains insufficient among multi-level social groups. From July to November 2016, we examined four potential functions of the relaxed open-mouth display during pairwise, intra-unit social interactions among 18 free-ranging adult and sub-adult golden snub-nosed monkeys(Rhinopithecus roxellana) who belonged to three one-male, multi-female units(OMU) at Dalongtan, Shennongjia National Park China. Results showed that: compared with no relaxed open-mouth display,(1) the occurrence of displacement by a dominant individual approaching a subordinate was lower and the distance of the subordinate to the approaching dominant was shorter when the subordinate showed open-mouth display;(2) relaxed open-mouth display reduced the probability of continued attack for victims of aggression and allowed victims to achieve closer proximity to the aggressor during post-conflict periods;(3) relaxed open-mouth display by dominant individuals allowed them to achieve closer proximity to subordinates; and(4) the exchange of relaxed open-mouth display had a greater impact on the outcome of interactions than one individual alone giving this signal. These findings suggest that relaxed open-mouth display serves important functions regarding submission, reconciliation, affiliation and reassurance in coordinating social interactions within OMUs in golden snub-nosed monkeys.
文摘Numerous plants and animals are edible and officinal,but some can be poisonous.There is sometimes confusion between poisonous and non-poisonous materials because of similarities in their morphologies.Consequently,the unwitting intake of poisonous plant or animal material has resulted in poisoning cases and sometimes in death,especially for situation in the wild.Rapid and accurate authentication of toxic species is essential for establishing and adopting optimal and urgent treatment for patients in such cases,and can be life-saving or can at least minimize the damage to health.Unfortunately,most of the current species authentication methods,including DNA barcoding,loop-mediated isothermal amplification(LAMP),chromatography technologies,and other methods,depend on professional equipment and a specialist laboratory,which are impracticable for real-time application in the field.It is therefore crucial to develop a rapid,accurate,and specific authentication method for poisonous species that does not require any equipment.
基金supported by the National Key Research and Development Program of China(2022YFD1300202)the Technology Innovation and Application Development Special Project of Chongqing(cstc2021jscx-gksb X0008)+2 种基金the National Natural Science Foundation of China(32102623)the National Natural Science Foundation of Chongqing(cstc2021jcyj-msxm X0875)the Ph D Train Scientific Research Project of Chongqing(CSTB2022BSXM-JCX0002)。
文摘Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process,and ovarian aging is shown by a decrease in the number and quality of oocytes.However,little is known about the molecular mechanisms of female age-related fertility decline in different types of ovarian cells during aging,especially in goats.Therefore,the aim of this study was to reveal the mechanisms driving ovarian aging in goats at single-cell resolution.Results For the first time,we surveyed the single-cell transcriptomic landscape of over 27,000 ovarian cells from newborn,young and aging goats,and identified nine ovarian cell types with distinct gene-expression signatures.Functional enrichment analysis showed that ovarian cell types were involved in their own unique biological processes,such as Wnt beta-catenin signalling was enriched in germ cells,whereas ovarian steroidogenesis was enriched in granulosa cells(GCs).Further analysis showed that ovarian aging was linked to GCs-specific changes in the antioxidant system,oxidative phosphorylation,and apoptosis.Subsequently,we identified a series of dynamic genes,such as AMH,CRABP2,THBS1 and TIMP1,which determined the fate of GCs.Additionally,FOXO1,SOX4,and HIF1A were identified as significant regulons that instructed the differentiation of GCs in a distinct manner during ovarian aging.Conclusions This study revealed a comprehensive aging-associated transcriptomic atlas characterizing the cell typespecific mechanisms during ovarian aging at the single-cell level and offers new diagnostic biomarkers and potential therapeutic targets for age-related goat ovarian diseases.
