Fetal intracranial tumors are rare,accounting for approximately 0.5%–1.9%of all pediatric tumors,though the true incidence may be underestimated.These tumors often present with distinct histopathological features,ima...Fetal intracranial tumors are rare,accounting for approximately 0.5%–1.9%of all pediatric tumors,though the true incidence may be underestimated.These tumors often present with distinct histopathological features,imaging characteristics,and clinical behavior compared to their postnatal counterparts.This review summarizes the current understanding of the prenatal diagnosis and characterization of fetal brain tumors,with a particular focus on the role of fetal magnetic resonance imaging(MRI).We discuss the advantages of advanced MR sequences in enhancing lesion detection and anatomical delineation following suspicious findings on obstetric ultrasound.Common tumor types encountered in utero—including teratomas,as-trocytomas,medulloblastomas,choroid plexus papillomas,and craniopharyngiomas—are reviewed in terms of imaging fea-tures,differential diagnosis,and clinical implications.Furthermore,the review addresses the diagnostic challenges,prognostic considerations,and the potential role of fetal MRI in guiding perinatal management and parental counseling.展开更多
Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases invo...Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases involved in angiogenesis[2].However,their involvement in regulating endothelial regeneration remains largely unknown.展开更多
With the aging global population,the decline in muscle mass and function among the elderly has emerged as a significant concern.This systemic progressive generalized loss of muscle function and mass is referred to as ...With the aging global population,the decline in muscle mass and function among the elderly has emerged as a significant concern.This systemic progressive generalized loss of muscle function and mass is referred to as sarcopenia(SP).In recent years,a growing number of studies have investigated SP,revealing that many tumor diseases,especially in the digestive system,promote its occurrence due to the influence of the disease itself,diet,and other factors.Moreover,SP patients tend to have poorer postoperative recovery.At present,many diagnostic methods have been developed for SP,but no unified standard has been established.Furthermore,the cutoff values of many diagnostic methods for different populations are still in the exploratory stage,and additional clinical studies are required to explore these issues.This article comprehensively and systematically summarizes the diagnostic methods and criteria mentioned in previous research,focusing on the impact of SP on post-surgical patients with various malignant tumors.展开更多
BACKGROUND Intramural pregnancy is a rare type of ectopic pregnancy,which is diagnosed by transvaginal ultrasound and magnetic resonance imaging.Management strategies vary depending on the site of the pregnancy,the ge...BACKGROUND Intramural pregnancy is a rare type of ectopic pregnancy,which is diagnosed by transvaginal ultrasound and magnetic resonance imaging.Management strategies vary depending on the site of the pregnancy,the gestational age and the desire to maintain fertility.The incidence of intramural pregnancy in assisted reproductive technology is higher than that in natural pregnancy.CASE SUMMARY We present a case of intramural pregnancy after in vitro fertilization and elective single embryo transfer following salpingectomy.The patient was completely asymptomatic and her serumβ-human chorionic gonadotropin level increased from 290 m IU/m L to 1759 m IU/m L.Three-dimensional transvaginal ultrasound indicated a heterogeneous echogenic mass arising from the uterine fundus which was surrounded by myometrium and a slender and extremely hypoechoic area stretching to the uterine cavity which was thought to be a fistulous tract.Therefore,we considered a diagnosis of intramural pregnancy and laparoscopic surgery was conducted at 7 wk gestation.CONCLUSION Early diagnosis and treatment of intramural pregnancy is significant for maintaining fertility.展开更多
A series of novel nitric oxide-donating sixalkoxyl biphenyl derivatives （14a-1） were synthesized by coupling furoxan with alkoxyl biphenyl skeleton using amino acids as the spacers, and their cytotoxicity against He...A series of novel nitric oxide-donating sixalkoxyl biphenyl derivatives （14a-1） were synthesized by coupling furoxan with alkoxyl biphenyl skeleton using amino acids as the spacers, and their cytotoxicity against HepG2 cells in vitro were evaluated by MTT method. It was found that 14c, 14d, 14f, 14i, 14j and 14k showed more potent cytotoxic activities than control 5-fluorouracil. NO release assay of target compounds indicated that the maximum amount of NO released by most active compounds 14c and 14j was about 6 × 10^-2 μmol/L, whereas 14a and 14h with very weak activity only released NO of 1 × 10^-2 μmol/L.展开更多
Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In o...Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In order to improve the prediction accuracy of ultra-deep well drilling costs,the item and the dominant factors of drilling costs in Tarim oilfield are analyzed.Then,those factors of drilling costs are separated into categorical variables and numerous variables.Finally,a BP neural networkmodel with drilling costs as the output is established,and hyper-parameters(initial weights and bias)of the BP neural network is optimized by genetic algorithm(GA).Through training and validation of themodel,a reliable prediction model of ultra-deep well drilling costs is achieved.The average relative error between prediction and actual values is 3.26%.Compared with other models,the root mean square error is reduced by 25.38%.The prediction results of the proposed model are reliable,and the model is efficient,which can provide supporting for the drilling costs control and budget planning of ultra-deep wells.展开更多
In search of novel anticancer agents, a series of thalidomide analogs （6a-j） were designed and synthesized. Cytotoxicity of these compounds against human hepatoma cells （HepG2） was evaluated by MTT method. Compoun...In search of novel anticancer agents, a series of thalidomide analogs （6a-j） were designed and synthesized. Cytotoxicity of these compounds against human hepatoma cells （HepG2） was evaluated by MTT method. Compounds 6d, 6h and 6i showed significant cytotoxic activities comparable to or stronger than control 5-fluorouracil.展开更多
Bupivacaine ranks as the most potent and efficient drug among class I local anesthetics, but its high potential for toxic reactions severely limits its clinical use. Although bupivacaine-induced toxicity is mainly cau...Bupivacaine ranks as the most potent and efficient drug among class I local anesthetics, but its high potential for toxic reactions severely limits its clinical use. Although bupivacaine-induced toxicity is mainly caused by substantial blockade of voltage-gated sodium channels （VGSCs）, how these hydrophobic molecules interact with the receptor sites to which they bind remains unclear. Navl.5 is the dominant isoform of VGSCs expressed in cardiac myocytes, and its dysfunction may be the cause of bupivacaine- triggered arrhythmia. Here, we investigated the effect of bupivacaine on Navl.5 within the clinical concentration range. The electrophysiological measurements on Navl.5 expressed in Xenopus oocytes showed that bupivacaine induced a voltage- and concentration-dependent blockade on the peak of/Na and the half-maximal inhibitory dose was 4.51 pmol/L. Consistent with other local anesthetics, bupivacaine also induced a use-dependent blockade on Navl.5 currents. The underlying mechanisms of this blockade may contribute to the fact that bupivacaine not only dose-dependently affected the gating kinetics of Nay1.5 but also accelerated the development of its open-state slow inactivation. These results extend our knowledge of the action of bupivacaine on cardiac sodium channels, and therefore contribute to the safer and more efficient clinical use of bupivacaine.展开更多
Novel phthalimide derivatives （4a, 4b and 6a-6e） were designed as hybrids of thalidomide and NO-ASA, and their chemical synthesis and in vitro biological activities were presented. The preliminary results showed tha...Novel phthalimide derivatives （4a, 4b and 6a-6e） were designed as hybrids of thalidomide and NO-ASA, and their chemical synthesis and in vitro biological activities were presented. The preliminary results showed that compared to thalidomide 4a and 4b exhibited enhanced activities against ECV304 and HepG2 cells, whereas 6e was more potent against HepG2 cells. 2007 Yi Hua Zhang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
[Objectives]To explore the effects of Guizhi Shaoyao Zhimu Decoction on laboratory indicators and immune functions of patients with rheumatoid arthritis.[Methods]A total of 110 patients with rheumatoid arthritis admit...[Objectives]To explore the effects of Guizhi Shaoyao Zhimu Decoction on laboratory indicators and immune functions of patients with rheumatoid arthritis.[Methods]A total of 110 patients with rheumatoid arthritis admitted to the Second Department of Orthopedics,the Affiliated Chinese and Western Medicine Hospital of Nanjing,Nanjing University of Chinese Medicine from December 2014 to December 2016 were selected as the research objects and divided into a control group and an observation group using the random number table method,with 55 cases in each group.The control group was treated with conventional western medicine,and the observation group was treated with Guizhi Shaoyao Zhimu Decoction on the basis of the control group.Both groups were treated for one month.The clinical effects,improvement of clinical symptoms,laboratory indicators and immune function indicator levels between the two groups were compared;the incidence of adverse reactions in the two groups was counted.[Results]The total effective rate of the observation group was 92.73%,higher than that of the control group(78.18%)(P<0.05).Compared with that before treatment,after one month of treatment,the grip strength of the two groups increased,and the observation group was greater than the control group(P<0.05);the morning stiffness time and 20 m walking time of both groups were shortened,and the observation group was shorter than the control group(P<0.05);the number of joint tenderness,joint swelling,ESR,MPV,PDW,serum CRP,plasma IgA,IgG,IgM,RF levels in both groups decreased,and the observation group was lower than the control group(P<0.05);the serum C3 levels in both groups increased,and the observation group was higher than the control group(P<0.05).During the treatment,the incidence of adverse reactions in the observation group was 0.00%,which was lower than that in the control group(10.91%,P<0.05).[Conclusions]Using Guizhi Shaoyao Zhimu Decoction to treat rheumatoid arthritis can improve the laboratory indicators of patients,and enhance their immune functions,improve the curative effect,and the safety is high.展开更多
With the development of the knowledge economy, social professional quality of talent has an increasingly demanding. Medical students is China＇ s medical and health undertakings reserve personnel, it plays an importan...With the development of the knowledge economy, social professional quality of talent has an increasingly demanding. Medical students is China＇ s medical and health undertakings reserve personnel, it plays an important role for better protection of people＇ s health by strengthening vocational personality training medical students and improving professional quality. Therefore, we must pay attention to the medical student＇ s career awareness, ethics, professionalism, career aspirations and career interests of education on medical students to develop a healthy personality of the medical profession, and lay the foundation for the sustainable development of medical and health services.展开更多
It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students...It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students will not make a fundamental achievement. Because it is not only an academic training but also the examination management and the humanities cultivation have a close connection. The humanities cultivation which is based on the core of virtue requires a good campus environment. Therefore, we should adjust to the medical students＇ humanities quality training requirements, making the targeted reform and innovation of examination management.展开更多
Increasing evidence indicates that oligodendrocyte(OL)numbers and myelin as a dynamic cellular compartment perform a key role in the maintenance of neuronal function.Inhibiting white matter(WM)demyelination or promoti...Increasing evidence indicates that oligodendrocyte(OL)numbers and myelin as a dynamic cellular compartment perform a key role in the maintenance of neuronal function.Inhibiting white matter(WM)demyelination or promoting remyelination has garnered interest for its potential therapeutic strategy against ischemic stroke.Our previous work has shown that low-intensity pulsed ultrasound(LIPUS)could improve stroke recovery.However,it is unclear whether LIPUS can maintain WM integrity early after stroke or promote late WM repair.This study evaluated the efficacy of LIPUS on WM repair and long-term neurologic recovery after stroke.Male adult C57BL/6 mice underwent a focal cerebral ischemia model and were randomized to receive ultrasound stimulation(30 min once daily for 14 days).The effect of LIPUS on sensorimotor function was assessed by modified neurological severity score,rotarod test,grip strength test,and gait analysis up to 28 days after stroke.We found that ischemic stroke-induced WM damage was severe on day 7 and partially recovered on day 28.LIPUS prevented neuronal and oligodendrocyte progenitor cell(OPC)death during the acute phase of stroke(d7),protected WM integrity,and reduced brain atrophy and tissue damage during the recovery phase(d28).To further confirm the effect of LIPUS on remyelination,we assessed the proliferation and differentiation of OPCs.We found that LIPUS did not increase the number of OPCs(PDGFRα^(+)or NG2^(+)),but markedly increased the number of newly produced mature OLs(APC^(+))and myelin protein levels.Mechanistically,LIPUS may promote OL maturation and remyelination by down-regulating the interleukin-17A/Notch1 signaling pathway.In summary,LIPUS can protect OLs and neurons early after stroke and promote long-term WM repair and functional recovery.LIPUS will be a viable strategy for the treatment of ischemic stroke in the future.展开更多
Several viral infections are often associated with fetal brain structural anomalies,including white matter lesions,cerebellar hypoplasia,temporal lobe lesions,ventricular dilatation,intraventricular septa,ependymal cy...Several viral infections are often associated with fetal brain structural anomalies,including white matter lesions,cerebellar hypoplasia,temporal lobe lesions,ventricular dilatation,intraventricular septa,ependymal cysts,and microcephaly,et al.Ultrasound(US)is the first modality of choice to evaluate fetal brain structural anomalies,however,Ultrasound is usually effected by maternal and fetal factors such as obesity,oligohydramnios and fetal head positions.Fetal MRI is not susceptible to the limitations as ultrasound with higher contrast resolution than prenatal ultrasound,directly visualize certain structures,such as developing brain parenchyma and further confirming US diagnosis and adding additional diagnostic information.This article reviews prenatal magnetic resonance imaging(MRI)diagnosis of fetal brain structural anomalies associated with viral infection.展开更多
基金supported by the Medical Innovation Research Special Project of Science and Technology Commission of Shanghai Municipality(Grant/Award Number:23Y11907800)Fundamental Research Funds for the Central Universities(Grant/Award Number:YG2023ZD22)Shanghai Key Laboratory of Child Brain and Development(Grant/Award Number:24dz2260100).
文摘Fetal intracranial tumors are rare,accounting for approximately 0.5%–1.9%of all pediatric tumors,though the true incidence may be underestimated.These tumors often present with distinct histopathological features,imaging characteristics,and clinical behavior compared to their postnatal counterparts.This review summarizes the current understanding of the prenatal diagnosis and characterization of fetal brain tumors,with a particular focus on the role of fetal magnetic resonance imaging(MRI).We discuss the advantages of advanced MR sequences in enhancing lesion detection and anatomical delineation following suspicious findings on obstetric ultrasound.Common tumor types encountered in utero—including teratomas,as-trocytomas,medulloblastomas,choroid plexus papillomas,and craniopharyngiomas—are reviewed in terms of imaging fea-tures,differential diagnosis,and clinical implications.Furthermore,the review addresses the diagnostic challenges,prognostic considerations,and the potential role of fetal MRI in guiding perinatal management and parental counseling.
基金supported by the National Natural Science Foundation of China(Grant Nos.:32171124,31871156,31971101,32271180,82272229,and 81471852)Hunan Provincial Natural Science Foundation of China(Grant No.:2021JJ31058).
文摘Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases involved in angiogenesis[2].However,their involvement in regulating endothelial regeneration remains largely unknown.
文摘With the aging global population,the decline in muscle mass and function among the elderly has emerged as a significant concern.This systemic progressive generalized loss of muscle function and mass is referred to as sarcopenia(SP).In recent years,a growing number of studies have investigated SP,revealing that many tumor diseases,especially in the digestive system,promote its occurrence due to the influence of the disease itself,diet,and other factors.Moreover,SP patients tend to have poorer postoperative recovery.At present,many diagnostic methods have been developed for SP,but no unified standard has been established.Furthermore,the cutoff values of many diagnostic methods for different populations are still in the exploratory stage,and additional clinical studies are required to explore these issues.This article comprehensively and systematically summarizes the diagnostic methods and criteria mentioned in previous research,focusing on the impact of SP on post-surgical patients with various malignant tumors.
基金Supported by National Natural Science Foundation of China,No. 81971386 and No. 81871210
文摘BACKGROUND Intramural pregnancy is a rare type of ectopic pregnancy,which is diagnosed by transvaginal ultrasound and magnetic resonance imaging.Management strategies vary depending on the site of the pregnancy,the gestational age and the desire to maintain fertility.The incidence of intramural pregnancy in assisted reproductive technology is higher than that in natural pregnancy.CASE SUMMARY We present a case of intramural pregnancy after in vitro fertilization and elective single embryo transfer following salpingectomy.The patient was completely asymptomatic and her serumβ-human chorionic gonadotropin level increased from 290 m IU/m L to 1759 m IU/m L.Three-dimensional transvaginal ultrasound indicated a heterogeneous echogenic mass arising from the uterine fundus which was surrounded by myometrium and a slender and extremely hypoechoic area stretching to the uterine cavity which was thought to be a fistulous tract.Therefore,we considered a diagnosis of intramural pregnancy and laparoscopic surgery was conducted at 7 wk gestation.CONCLUSION Early diagnosis and treatment of intramural pregnancy is significant for maintaining fertility.
文摘A series of novel nitric oxide-donating sixalkoxyl biphenyl derivatives （14a-1） were synthesized by coupling furoxan with alkoxyl biphenyl skeleton using amino acids as the spacers, and their cytotoxicity against HepG2 cells in vitro were evaluated by MTT method. It was found that 14c, 14d, 14f, 14i, 14j and 14k showed more potent cytotoxic activities than control 5-fluorouracil. NO release assay of target compounds indicated that the maximum amount of NO released by most active compounds 14c and 14j was about 6 × 10^-2 μmol/L, whereas 14a and 14h with very weak activity only released NO of 1 × 10^-2 μmol/L.
基金supported by the Science and Technology Innovation Foundation of CNPC“Multiscale Flow Law and Flow Field Coupling Study of Tight Sandstone Gas Reservoir”(2016D-5007-0208)13th Five-Year National Major Project“Multistage Fracturing Effect and Production of Fuling Shale Gas HorizontalWell Law Analysis Research”(2016ZX05060-009).
文摘Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In order to improve the prediction accuracy of ultra-deep well drilling costs,the item and the dominant factors of drilling costs in Tarim oilfield are analyzed.Then,those factors of drilling costs are separated into categorical variables and numerous variables.Finally,a BP neural networkmodel with drilling costs as the output is established,and hyper-parameters(initial weights and bias)of the BP neural network is optimized by genetic algorithm(GA).Through training and validation of themodel,a reliable prediction model of ultra-deep well drilling costs is achieved.The average relative error between prediction and actual values is 3.26%.Compared with other models,the root mean square error is reduced by 25.38%.The prediction results of the proposed model are reliable,and the model is efficient,which can provide supporting for the drilling costs control and budget planning of ultra-deep wells.
文摘In search of novel anticancer agents, a series of thalidomide analogs （6a-j） were designed and synthesized. Cytotoxicity of these compounds against human hepatoma cells （HepG2） was evaluated by MTT method. Compounds 6d, 6h and 6i showed significant cytotoxic activities comparable to or stronger than control 5-fluorouracil.
基金supported by grants from the National Natural Science Foundation of China (31171064)the Key Research Program of Science and Technology Commissions of Shanghai Municipality (11JC1404300,13DJ1400300)with financial support from Xinhua Hospital (Chongming),Shanghai Jiaotong University School of Medicine
文摘Bupivacaine ranks as the most potent and efficient drug among class I local anesthetics, but its high potential for toxic reactions severely limits its clinical use. Although bupivacaine-induced toxicity is mainly caused by substantial blockade of voltage-gated sodium channels （VGSCs）, how these hydrophobic molecules interact with the receptor sites to which they bind remains unclear. Navl.5 is the dominant isoform of VGSCs expressed in cardiac myocytes, and its dysfunction may be the cause of bupivacaine- triggered arrhythmia. Here, we investigated the effect of bupivacaine on Navl.5 within the clinical concentration range. The electrophysiological measurements on Navl.5 expressed in Xenopus oocytes showed that bupivacaine induced a voltage- and concentration-dependent blockade on the peak of/Na and the half-maximal inhibitory dose was 4.51 pmol/L. Consistent with other local anesthetics, bupivacaine also induced a use-dependent blockade on Navl.5 currents. The underlying mechanisms of this blockade may contribute to the fact that bupivacaine not only dose-dependently affected the gating kinetics of Nay1.5 but also accelerated the development of its open-state slow inactivation. These results extend our knowledge of the action of bupivacaine on cardiac sodium channels, and therefore contribute to the safer and more efficient clinical use of bupivacaine.
文摘Novel phthalimide derivatives （4a, 4b and 6a-6e） were designed as hybrids of thalidomide and NO-ASA, and their chemical synthesis and in vitro biological activities were presented. The preliminary results showed that compared to thalidomide 4a and 4b exhibited enhanced activities against ECV304 and HepG2 cells, whereas 6e was more potent against HepG2 cells. 2007 Yi Hua Zhang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金Supported by the 12th Five-Year TCM Key Discipline Construction Project of Jiangsu Province(Su Zhong Yi Zheng[2013]No.30).
文摘[Objectives]To explore the effects of Guizhi Shaoyao Zhimu Decoction on laboratory indicators and immune functions of patients with rheumatoid arthritis.[Methods]A total of 110 patients with rheumatoid arthritis admitted to the Second Department of Orthopedics,the Affiliated Chinese and Western Medicine Hospital of Nanjing,Nanjing University of Chinese Medicine from December 2014 to December 2016 were selected as the research objects and divided into a control group and an observation group using the random number table method,with 55 cases in each group.The control group was treated with conventional western medicine,and the observation group was treated with Guizhi Shaoyao Zhimu Decoction on the basis of the control group.Both groups were treated for one month.The clinical effects,improvement of clinical symptoms,laboratory indicators and immune function indicator levels between the two groups were compared;the incidence of adverse reactions in the two groups was counted.[Results]The total effective rate of the observation group was 92.73%,higher than that of the control group(78.18%)(P<0.05).Compared with that before treatment,after one month of treatment,the grip strength of the two groups increased,and the observation group was greater than the control group(P<0.05);the morning stiffness time and 20 m walking time of both groups were shortened,and the observation group was shorter than the control group(P<0.05);the number of joint tenderness,joint swelling,ESR,MPV,PDW,serum CRP,plasma IgA,IgG,IgM,RF levels in both groups decreased,and the observation group was lower than the control group(P<0.05);the serum C3 levels in both groups increased,and the observation group was higher than the control group(P<0.05).During the treatment,the incidence of adverse reactions in the observation group was 0.00%,which was lower than that in the control group(10.91%,P<0.05).[Conclusions]Using Guizhi Shaoyao Zhimu Decoction to treat rheumatoid arthritis can improve the laboratory indicators of patients,and enhance their immune functions,improve the curative effect,and the safety is high.
文摘With the development of the knowledge economy, social professional quality of talent has an increasingly demanding. Medical students is China＇ s medical and health undertakings reserve personnel, it plays an important role for better protection of people＇ s health by strengthening vocational personality training medical students and improving professional quality. Therefore, we must pay attention to the medical student＇ s career awareness, ethics, professionalism, career aspirations and career interests of education on medical students to develop a healthy personality of the medical profession, and lay the foundation for the sustainable development of medical and health services.
文摘It has been proved by years of research that if the function of examination management cannot be fully understood or be used effectively, the humanities education and train of professional quality for medical students will not make a fundamental achievement. Because it is not only an academic training but also the examination management and the humanities cultivation have a close connection. The humanities cultivation which is based on the core of virtue requires a good campus environment. Therefore, we should adjust to the medical students＇ humanities quality training requirements, making the targeted reform and innovation of examination management.
基金supported by grants from the National Natural Science Foundation of China(nos.81974184 and 82271366)the Hebei Key Laboratory of Vascular Homeostasis(20567630H)+1 种基金the“14th Five-Year Plan”Clinical Medicine Innovation Research Team Support Plan of Hebei Medical University(no.9-B by Chen Rong)the Health Commission of Hebei Province(20190061).
文摘Increasing evidence indicates that oligodendrocyte(OL)numbers and myelin as a dynamic cellular compartment perform a key role in the maintenance of neuronal function.Inhibiting white matter(WM)demyelination or promoting remyelination has garnered interest for its potential therapeutic strategy against ischemic stroke.Our previous work has shown that low-intensity pulsed ultrasound(LIPUS)could improve stroke recovery.However,it is unclear whether LIPUS can maintain WM integrity early after stroke or promote late WM repair.This study evaluated the efficacy of LIPUS on WM repair and long-term neurologic recovery after stroke.Male adult C57BL/6 mice underwent a focal cerebral ischemia model and were randomized to receive ultrasound stimulation(30 min once daily for 14 days).The effect of LIPUS on sensorimotor function was assessed by modified neurological severity score,rotarod test,grip strength test,and gait analysis up to 28 days after stroke.We found that ischemic stroke-induced WM damage was severe on day 7 and partially recovered on day 28.LIPUS prevented neuronal and oligodendrocyte progenitor cell(OPC)death during the acute phase of stroke(d7),protected WM integrity,and reduced brain atrophy and tissue damage during the recovery phase(d28).To further confirm the effect of LIPUS on remyelination,we assessed the proliferation and differentiation of OPCs.We found that LIPUS did not increase the number of OPCs(PDGFRα^(+)or NG2^(+)),but markedly increased the number of newly produced mature OLs(APC^(+))and myelin protein levels.Mechanistically,LIPUS may promote OL maturation and remyelination by down-regulating the interleukin-17A/Notch1 signaling pathway.In summary,LIPUS can protect OLs and neurons early after stroke and promote long-term WM repair and functional recovery.LIPUS will be a viable strategy for the treatment of ischemic stroke in the future.
基金supported by[National Natural Science Foundation of China]under grant[number 81101032][National Natural Science Foundation of China]under grant[number 81571628][Shanghai Municipal Commission of Health and Family Planning]under grant[number 201540048].
文摘Several viral infections are often associated with fetal brain structural anomalies,including white matter lesions,cerebellar hypoplasia,temporal lobe lesions,ventricular dilatation,intraventricular septa,ependymal cysts,and microcephaly,et al.Ultrasound(US)is the first modality of choice to evaluate fetal brain structural anomalies,however,Ultrasound is usually effected by maternal and fetal factors such as obesity,oligohydramnios and fetal head positions.Fetal MRI is not susceptible to the limitations as ultrasound with higher contrast resolution than prenatal ultrasound,directly visualize certain structures,such as developing brain parenchyma and further confirming US diagnosis and adding additional diagnostic information.This article reviews prenatal magnetic resonance imaging(MRI)diagnosis of fetal brain structural anomalies associated with viral infection.
