Under off-season production mode, change laws of nutritive materials in leaves of fruiting mother branches of mango in flowering process induced by dif- ferent agents were investigated. The results showed that the flo...Under off-season production mode, change laws of nutritive materials in leaves of fruiting mother branches of mango in flowering process induced by dif- ferent agents were investigated. The results showed that the flowering time of manga trees in the potassium nitrate treatment was earlier than the ethephon treatment by 7 d, and changes trends of materials in leaves of the potassium nitrate and ethephon treatments were substantially the same. The nutritive materials in leaves showed trends of increasing at first and decreasing then. In early flower bud differentiation stage, soluble sugar and starch in leaves increased rapidly, and content of soluble protein also increased rapidly and showed its their peak values, thereby providing energy substances and structural substances demanded by flower bud formation. With flower bud differentiation going on, soluble sugar, starch and soluble protein decreased gradually. It was indicated that the accumulation of soluble sugar, starch and soluble protein is beneficial to flower bud differentiation.展开更多
This study was conducted to investigate changes in the expression of AP1 gene in flowering process. Potassium nitrate and ethephon were sprayed on 7- year-old Guifei trees out of season. The results showed that AP1 ge...This study was conducted to investigate changes in the expression of AP1 gene in flowering process. Potassium nitrate and ethephon were sprayed on 7- year-old Guifei trees out of season. The results showed that AP1 gene had a higher expression level in terminal buds, and especially, the expression level increased significantly in late stage of flower bud differentiation. Potassium nitrate and ethephon promoted flower bud differentiation, and the expression level of AP1 gene in- creased in flowering process remarkably. Expression ofAP1 gene of the potassium nitrate treatment was significantly greater than that of the ethephon treatment and the CK.展开更多
The integration of strong near-infrared(NIR)emission,rapid lysosome escape,fast cellular excretion,and efficient total body clearance is highly desired for nanoparticles(NPs)to achieve synergistic functions in both mo...The integration of strong near-infrared(NIR)emission,rapid lysosome escape,fast cellular excretion,and efficient total body clearance is highly desired for nanoparticles(NPs)to achieve synergistic functions in both molecular imaging and delivery.Herein,using a well-designed cyclopeptide(CP)that can spontaneously assem ble into controllable nanofibers a s template,a facile strategy is reported for in situ self-assembly of NIR-emitting gold NPs(AuNPs)into ordered and well-controlled one-dimensional(1D)nanostructures(AuNPs@CP)with greatly enhanced NIR emission(〜6 fold).Comparing with the unassem bled AuNPs,the AuNPs@CP are observed to enter living cells through endocytosis,escap e from lysosome rapidly,and excrete the cell fast,which shows high gene transfection efficiencies in construction of cell line with-7.5-fold overexpression of p53 protein.Furthermore,the AuNPs@CP exhibit high in vivo diffusibility and total body clearance efficiency with minimized healthy organ retention,which are also demonstrated to be good nanovectors for plasmid complementary deoxyribonucleic acid 3.1(pcDNA3.1)(+)-internal ribosome entry site(IRES)-green fluorescent protein(GFP)-p53 plasmid with efficient p53 gene over-expression in tumor site.This facile in situ strategy in fabricating highly luminescent 1D nanostructures provides a promising approach toward future translatable multifunctional nanostructures for delivering,tracking,and therapy.展开更多
The understanding of amphiphilic block copolymers(ABC)in encapsulation and transport of inorganic nanomedicines is highly desired.Still,it remains limited due to the challenges in the fabrication of nanoassemblies(NAs...The understanding of amphiphilic block copolymers(ABC)in encapsulation and transport of inorganic nanomedicines is highly desired.Still,it remains limited due to the challenges in the fabrication of nanoassemblies(NAs)with highly-controlled shape and loading of nanoparticles.Herein,through growth regulation of luminescent gold nanoparticles(Au NPs)by different reductants with ABC pluronic F127 as a template,a straightforward strategy is reported for in-situ fabrication of three wellcontrolled gold NAs(Au NAs)that display tunable shapes from spherical to elongated nanostructures and controllable surface chemistry and loading of Au NPs with distinct emissions but identical individual Au NP size.The three Au NAs exhibit tailored invivo transport behaviours:those with spherical shape and more hydrophilic surface show longer blood retention with higher tumor-targeting efficiency(~25.3%injection dose/g)and excellent long-term near-infrared tumor imaging even after 96 h postinjection.These findings provide a useful guidance in designing specific nanostructures for future nanomedicine transport.展开更多
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032013010)Special Fund for Agro-scientific Research in the Public Interest(201203092)
文摘Under off-season production mode, change laws of nutritive materials in leaves of fruiting mother branches of mango in flowering process induced by dif- ferent agents were investigated. The results showed that the flowering time of manga trees in the potassium nitrate treatment was earlier than the ethephon treatment by 7 d, and changes trends of materials in leaves of the potassium nitrate and ethephon treatments were substantially the same. The nutritive materials in leaves showed trends of increasing at first and decreasing then. In early flower bud differentiation stage, soluble sugar and starch in leaves increased rapidly, and content of soluble protein also increased rapidly and showed its their peak values, thereby providing energy substances and structural substances demanded by flower bud formation. With flower bud differentiation going on, soluble sugar, starch and soluble protein decreased gradually. It was indicated that the accumulation of soluble sugar, starch and soluble protein is beneficial to flower bud differentiation.
基金Supported by CATAS-TCGRI(1630032013010)Special Fund for Agro-scientific Research in the Public Interest(201203092)
文摘This study was conducted to investigate changes in the expression of AP1 gene in flowering process. Potassium nitrate and ethephon were sprayed on 7- year-old Guifei trees out of season. The results showed that AP1 gene had a higher expression level in terminal buds, and especially, the expression level increased significantly in late stage of flower bud differentiation. Potassium nitrate and ethephon promoted flower bud differentiation, and the expression level of AP1 gene in- creased in flowering process remarkably. Expression ofAP1 gene of the potassium nitrate treatment was significantly greater than that of the ethephon treatment and the CK.
基金the National Natural Science Foundation of China(Nos.21573078,22022403)Guangdong Natural Science Funds for Distinguished Young Scholars(No.2016A030306024)+1 种基金Guangzhou Science and Technology Project(No.201904010055)Fundamental Research Funds for the Central Universities.
文摘The integration of strong near-infrared(NIR)emission,rapid lysosome escape,fast cellular excretion,and efficient total body clearance is highly desired for nanoparticles(NPs)to achieve synergistic functions in both molecular imaging and delivery.Herein,using a well-designed cyclopeptide(CP)that can spontaneously assem ble into controllable nanofibers a s template,a facile strategy is reported for in situ self-assembly of NIR-emitting gold NPs(AuNPs)into ordered and well-controlled one-dimensional(1D)nanostructures(AuNPs@CP)with greatly enhanced NIR emission(〜6 fold).Comparing with the unassem bled AuNPs,the AuNPs@CP are observed to enter living cells through endocytosis,escap e from lysosome rapidly,and excrete the cell fast,which shows high gene transfection efficiencies in construction of cell line with-7.5-fold overexpression of p53 protein.Furthermore,the AuNPs@CP exhibit high in vivo diffusibility and total body clearance efficiency with minimized healthy organ retention,which are also demonstrated to be good nanovectors for plasmid complementary deoxyribonucleic acid 3.1(pcDNA3.1)(+)-internal ribosome entry site(IRES)-green fluorescent protein(GFP)-p53 plasmid with efficient p53 gene over-expression in tumor site.This facile in situ strategy in fabricating highly luminescent 1D nanostructures provides a promising approach toward future translatable multifunctional nanostructures for delivering,tracking,and therapy.
基金the National Natural Science Foundation of China(21573078,21907032,22022403)Guangdong Natural Science Funds for Distinguished Young Scholars(2016A030306024)+1 种基金the Natural Science Foundation of Guangdong Province(2018A030310376)Guangzhou Science and Technology Project(201904010055)。
文摘The understanding of amphiphilic block copolymers(ABC)in encapsulation and transport of inorganic nanomedicines is highly desired.Still,it remains limited due to the challenges in the fabrication of nanoassemblies(NAs)with highly-controlled shape and loading of nanoparticles.Herein,through growth regulation of luminescent gold nanoparticles(Au NPs)by different reductants with ABC pluronic F127 as a template,a straightforward strategy is reported for in-situ fabrication of three wellcontrolled gold NAs(Au NAs)that display tunable shapes from spherical to elongated nanostructures and controllable surface chemistry and loading of Au NPs with distinct emissions but identical individual Au NP size.The three Au NAs exhibit tailored invivo transport behaviours:those with spherical shape and more hydrophilic surface show longer blood retention with higher tumor-targeting efficiency(~25.3%injection dose/g)and excellent long-term near-infrared tumor imaging even after 96 h postinjection.These findings provide a useful guidance in designing specific nanostructures for future nanomedicine transport.
