TIME,Immunity,Prognosis,BioinformaticsThis study used transcriptome and epigenetic data to predict the prognosis of immune-related genes(IRGs)Apelin(APLN)in patients with hepatocellular carcinoma(HCC).The TCGA databas...TIME,Immunity,Prognosis,BioinformaticsThis study used transcriptome and epigenetic data to predict the prognosis of immune-related genes(IRGs)Apelin(APLN)in patients with hepatocellular carcinoma(HCC).The TCGA database has gene expression and clinical data for HCC.And DNA methylation 450 k data for HCC was download from the University of California Santa Cruz(UCSC)Xena browser.Performing clinical and prognostic analysis of APLN expression,results show that APLN is highly expressed in tumor samples.And it has an increasing trend with the development of clinical stage and T stage.To explore the prognostic role of APLN,the Immune-related DNA methylation(DNAm)sites associated with APLN analyzed by bioinformatics.Univariate COX screened the methylation sites that are related to both APLN and survival.The risk score related to methylation site signature was determined according to their least absolute shrinkage and selection operator(LASSO)coefficients.Then the patients were divided into high-risk groups and low-risk groups.Significant differences in overall survival(OS)were found in the training cohort.Nomogram shows that APLN or methylation signature can effectively predict the prognosis of HCC patients.In summary,APLN may be a diagnostic and prognostic marker for HCC.展开更多
The medical fungus Hirsutella sinensis has been used as a Chinese folk health supplement because of its immunomodulatory properties.Our previous studies established the antifibrotic action of Hirsutella sinensis mycel...The medical fungus Hirsutella sinensis has been used as a Chinese folk health supplement because of its immunomodulatory properties.Our previous studies established the antifibrotic action of Hirsutella sinensis mycelium(HSM)in the lung.The epithelial–mesenchymal transition(EMT)is involved in the pathogenesis of idiopathic pulmonary fibrosis.The present study investigates the role of HSM in mediating EMT during the development of pulmonary fibrosis.HSM significantly inhibits bleomycin(BLM)-induced pulmonary fibrosis by blocking the EMT.In addition,the expression levels of midkine are increased in the lungs of the BLM-induced group.Further analysis of the results indicates that the mRNA level of midkine correlated positively with EMT.HSM markedly abrogates the transforming growth factorβ-induced EMT-like phenotype and behavior in vitro.The activation of midkine related signaling pathway is ameliorated following HSM treatment,whereas this extract also caused an effective attenuation of the induction of EMT(caused by midkine overexpression)in vitro.Results further confirm that oral medication of HSM disrupted the midkine pathway in vivo.Overall,findings suggest that the midkine pathway and the regulation of the EMT may be considered novel candidate therapeutic targets for the antifibrotic effects caused by HSM.展开更多
Trauma is one of the leading causes of death worldwide. Approximately 39.5% of deaths occur in the hospital, and the mortality rate of delayed death caused by septic complications is still high. Early prediction of th...Trauma is one of the leading causes of death worldwide. Approximately 39.5% of deaths occur in the hospital, and the mortality rate of delayed death caused by septic complications is still high. Early prediction of the development of sepsis can help promote early intervention and treatment for patients and contribute to improving patient outcomes. Thus so far, biomarkers, patient demo-graphics and injury characteristics are the main methods used for predicting sepsis in trauma patients. However, studies that verify their predictive value are limited, and the results are still controversial. More work should be conducted to explore more efcient and accurate ways to predict post-traumatic sepsis.展开更多
Developing novel antibacterial dressing protecting skin injuries from infection is essential for wound healing.In this study,sericin,a bio-waste produced during the degumming of silk cocoons,is utilized to exfoliate M...Developing novel antibacterial dressing protecting skin injuries from infection is essential for wound healing.In this study,sericin,a bio-waste produced during the degumming of silk cocoons,is utilized to exfoliate MoS_(2)layers and improve the dispersity and stability of MoS_(2)nanosheets(MoS_(2)-NSs).Moreover,owing to its ability to promote oxygen permeability and cell growth and its good biocompatibility,MoS_(2)-NS/Sericin maintains its photothermal property under an 808 nm light source for a strong antibacterial activity as well as improves the fibroblast migration,which accelerates wound healing.Fur-thermore,the in vitro experiments indicates that MoS_(2)-NS/Sericin can also scavenge reactive oxygen species(ROS)at an inflammatory stage of wound healing and transform classical activated macrophages(M1-type)into alternatively activated macrophages(M2-type),which is beneficial for wound recovery.Based on these results observed in vitro,full-thickness skin wound experiments are conducted on rats,and the corresponding results show that MoS_(2)/Sericin under 808 nm irradiation exhibits the best performance in promoting wound healing.Overall,MoS_(2)-NS/Sericin exhibits a high potential for bacteria-infected wound healing.展开更多
To the Editor Identification upon admission of a group of patients with severe traumatic injury presenting with a higher risk of death is crucial in designing personalized treatment strategies[1].However,there are as ...To the Editor Identification upon admission of a group of patients with severe traumatic injury presenting with a higher risk of death is crucial in designing personalized treatment strategies[1].However,there are as yet limited biomarkers for the prediction of mortality in patients with traumatic injury.12(S)-Hydroxyeicosatetraenoic acid(12(S)-HETE),as a classic metabolite of arachidonic acid,was suggested to be a potential biomarker in brain injury[2]or subarachnoid hemorrhage[3].展开更多
基金This work was supported by the Fund of Biosecurity Specialized Project of PLA(No.19SWAQ18).
文摘TIME,Immunity,Prognosis,BioinformaticsThis study used transcriptome and epigenetic data to predict the prognosis of immune-related genes(IRGs)Apelin(APLN)in patients with hepatocellular carcinoma(HCC).The TCGA database has gene expression and clinical data for HCC.And DNA methylation 450 k data for HCC was download from the University of California Santa Cruz(UCSC)Xena browser.Performing clinical and prognostic analysis of APLN expression,results show that APLN is highly expressed in tumor samples.And it has an increasing trend with the development of clinical stage and T stage.To explore the prognostic role of APLN,the Immune-related DNA methylation(DNAm)sites associated with APLN analyzed by bioinformatics.Univariate COX screened the methylation sites that are related to both APLN and survival.The risk score related to methylation site signature was determined according to their least absolute shrinkage and selection operator(LASSO)coefficients.Then the patients were divided into high-risk groups and low-risk groups.Significant differences in overall survival(OS)were found in the training cohort.Nomogram shows that APLN or methylation signature can effectively predict the prognosis of HCC patients.In summary,APLN may be a diagnostic and prognostic marker for HCC.
基金the Six Talent Peaks Project in Jiangsu Province(No.YY-021)the Fundamental Research Funds of the Central Universities(No.021414380342)the Fund of State Key Laboratory of Trauma Burns and Combined Injury(No.SKLKF201602)。
文摘The medical fungus Hirsutella sinensis has been used as a Chinese folk health supplement because of its immunomodulatory properties.Our previous studies established the antifibrotic action of Hirsutella sinensis mycelium(HSM)in the lung.The epithelial–mesenchymal transition(EMT)is involved in the pathogenesis of idiopathic pulmonary fibrosis.The present study investigates the role of HSM in mediating EMT during the development of pulmonary fibrosis.HSM significantly inhibits bleomycin(BLM)-induced pulmonary fibrosis by blocking the EMT.In addition,the expression levels of midkine are increased in the lungs of the BLM-induced group.Further analysis of the results indicates that the mRNA level of midkine correlated positively with EMT.HSM markedly abrogates the transforming growth factorβ-induced EMT-like phenotype and behavior in vitro.The activation of midkine related signaling pathway is ameliorated following HSM treatment,whereas this extract also caused an effective attenuation of the induction of EMT(caused by midkine overexpression)in vitro.Results further confirm that oral medication of HSM disrupted the midkine pathway in vivo.Overall,findings suggest that the midkine pathway and the regulation of the EMT may be considered novel candidate therapeutic targets for the antifibrotic effects caused by HSM.
基金This study was supported by grants from the key project of"twelfth five-year plan" for medical science and technology of PLA (BWS11J038)
文摘Trauma is one of the leading causes of death worldwide. Approximately 39.5% of deaths occur in the hospital, and the mortality rate of delayed death caused by septic complications is still high. Early prediction of the development of sepsis can help promote early intervention and treatment for patients and contribute to improving patient outcomes. Thus so far, biomarkers, patient demo-graphics and injury characteristics are the main methods used for predicting sepsis in trauma patients. However, studies that verify their predictive value are limited, and the results are still controversial. More work should be conducted to explore more efcient and accurate ways to predict post-traumatic sepsis.
基金supported by the National Natural Science Foundation of China(No.22008201)Natural Science Foundation Innovation and Development Joint Fund Project of Chongqing(No.CSTB2023NSCQ-LZX0028)+2 种基金Fundamental Research Funds for the Central Universities(No.SWU-KW22004)Special Program Project(No.2022-JCJQ-ZD-224-12)Open Project Program of the Ministry of Education of the Key Laboratory of Textile Fiber and Products(No.Fzxw2021001).
文摘Developing novel antibacterial dressing protecting skin injuries from infection is essential for wound healing.In this study,sericin,a bio-waste produced during the degumming of silk cocoons,is utilized to exfoliate MoS_(2)layers and improve the dispersity and stability of MoS_(2)nanosheets(MoS_(2)-NSs).Moreover,owing to its ability to promote oxygen permeability and cell growth and its good biocompatibility,MoS_(2)-NS/Sericin maintains its photothermal property under an 808 nm light source for a strong antibacterial activity as well as improves the fibroblast migration,which accelerates wound healing.Fur-thermore,the in vitro experiments indicates that MoS_(2)-NS/Sericin can also scavenge reactive oxygen species(ROS)at an inflammatory stage of wound healing and transform classical activated macrophages(M1-type)into alternatively activated macrophages(M2-type),which is beneficial for wound recovery.Based on these results observed in vitro,full-thickness skin wound experiments are conducted on rats,and the corresponding results show that MoS_(2)/Sericin under 808 nm irradiation exhibits the best performance in promoting wound healing.Overall,MoS_(2)-NS/Sericin exhibits a high potential for bacteria-infected wound healing.
文摘To the Editor Identification upon admission of a group of patients with severe traumatic injury presenting with a higher risk of death is crucial in designing personalized treatment strategies[1].However,there are as yet limited biomarkers for the prediction of mortality in patients with traumatic injury.12(S)-Hydroxyeicosatetraenoic acid(12(S)-HETE),as a classic metabolite of arachidonic acid,was suggested to be a potential biomarker in brain injury[2]or subarachnoid hemorrhage[3].
