A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were w...A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were withering time,fermentation time and fermentation temperature.Each factor was set by three levels.The responses were predicted by analysis of the regression equation.In the study,the models of regression equation were significant by the analysis of variance(ANOVA).The second-order response surface figures were showed that interaction term is correlated with the response.Optimal process parameters for the maximum of TF and TR formation were obtained at withering time of 12 hours,fermentation time of 2 hours,and fermentation temperature of 30℃.The predictions of TF and TR formation were 0.52% and 3.98%.The results obtained in this study showed that the CCD and Response Surface Methodology(RSM) could be an effective tool to optimize the process parameters of Congou black tea manufacture.展开更多
The growing importance of peptides and proteins in therapeutic and biomedical applications has provided immense motivation toward the development of new ways to construct and transform peptide molecules.As in other ar...The growing importance of peptides and proteins in therapeutic and biomedical applications has provided immense motivation toward the development of new ways to construct and transform peptide molecules.As in other areas of organic synthesis,C–H functionalization(CHF)chemistry could potentially exemplify disruptive technologies for peptide engineering.Over the past decade,the field has witnessed an exciting surge of reports of various metal-catalyzed CHF chemistry for postassembly modification of peptides and proteins.This review chronicles present advances in this research area up to June 2020.The content is organized based on the location of CHF on peptides:amino acid side chains(aromatic and nonaromatic),backbone,and appendant groups on peptide terminus.In addition to the reaction mechanisms of the metal-catalyzed CHF chemistry used in these peptide modification protocols,brief comments on the corresponding nonmetal-mediated strategies are included to provide readers a broad view of the current status of CHF-enabled peptide modification.展开更多
基金Supported by the Pillar Program of the Ministry of Science and Technology of China 2012BAF07B05
文摘A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were withering time,fermentation time and fermentation temperature.Each factor was set by three levels.The responses were predicted by analysis of the regression equation.In the study,the models of regression equation were significant by the analysis of variance(ANOVA).The second-order response surface figures were showed that interaction term is correlated with the response.Optimal process parameters for the maximum of TF and TR formation were obtained at withering time of 12 hours,fermentation time of 2 hours,and fermentation temperature of 30℃.The predictions of TF and TR formation were 0.52% and 3.98%.The results obtained in this study showed that the CCD and Response Surface Methodology(RSM) could be an effective tool to optimize the process parameters of Congou black tea manufacture.
基金The authors acknowledge the Natural Science Foundation of China(nos.91753124 and 21725204) financial support of this work.
文摘The growing importance of peptides and proteins in therapeutic and biomedical applications has provided immense motivation toward the development of new ways to construct and transform peptide molecules.As in other areas of organic synthesis,C–H functionalization(CHF)chemistry could potentially exemplify disruptive technologies for peptide engineering.Over the past decade,the field has witnessed an exciting surge of reports of various metal-catalyzed CHF chemistry for postassembly modification of peptides and proteins.This review chronicles present advances in this research area up to June 2020.The content is organized based on the location of CHF on peptides:amino acid side chains(aromatic and nonaromatic),backbone,and appendant groups on peptide terminus.In addition to the reaction mechanisms of the metal-catalyzed CHF chemistry used in these peptide modification protocols,brief comments on the corresponding nonmetal-mediated strategies are included to provide readers a broad view of the current status of CHF-enabled peptide modification.
