The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic an...The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.展开更多
178 cases of laparoscopic cholecystectomy performed in our hospital,during january 1993 to June 1995,had been studied,include 12 cases of conversion. There was no death,nobinary injury or other severe complication. Th...178 cases of laparoscopic cholecystectomy performed in our hospital,during january 1993 to June 1995,had been studied,include 12 cases of conversion. There was no death,nobinary injury or other severe complication. The average operative time was 56. 5±21. 3 minutes,average postroperative hospitalization was 5. 6 days. The indication and contraindition are discussed. The study supports that laparoscopic cholecystectomy is a safe and effective operation,themost complications can be prevented by accumul ative experiences and innocative instruments.展开更多
文摘The WSC proteins produced by Penicillium expansum play a crucial role in causing blue mold on pears.To analyze the role of the WSC1 gene in the pathogenic process of this fungal pathogen,we conducted transcriptomic analysis of a WSC1 knockout(ΔWSC1)strain.The knockout of WSC1 significantly altered the gene expression profile in P.expansum,particularly for genes involved in cell wall integrity,signaling,stress response,and toxin production.The differential expression of these genes might make theΔWSC1 strain more vulnerable to environmental stress,while reducing the toxin production capacity,ultimately leading to a decrease in the pathogenicity.The transcriptomic analysis revealed that the expression of genes related to stress response signals,defense mechanisms and oxidative stress management changed when pear fruits were infected with theΔWSC1 strain.These changes may trigger a cascade of responses in pear fruits.In addition,compared with those infected with the wild-type strain,pear fruits infected with theΔWSC1 strain exhibited up-regulated expression of genes related to defense and oxidative stress.This study clarifies how the WSC1 gene influences P.expansum’s ability to infect pear fruits and how pear fruits respond to the infection.
文摘178 cases of laparoscopic cholecystectomy performed in our hospital,during january 1993 to June 1995,had been studied,include 12 cases of conversion. There was no death,nobinary injury or other severe complication. The average operative time was 56. 5±21. 3 minutes,average postroperative hospitalization was 5. 6 days. The indication and contraindition are discussed. The study supports that laparoscopic cholecystectomy is a safe and effective operation,themost complications can be prevented by accumul ative experiences and innocative instruments.
