Objective: To explore the effect of salinomycin on the metastasis and invasion of bladder cancer cell line T24 by regulating the related protein expression in the process of epithelialmesenchymal transition(EMT), and ...Objective: To explore the effect of salinomycin on the metastasis and invasion of bladder cancer cell line T24 by regulating the related protein expression in the process of epithelialmesenchymal transition(EMT), and to provide experimental basis for the treatment of urological tumors. Methods: The bladder cancer cell line T24 was cultured in vitro. The rat bladder tumor model was established in vivo. The rats were randomized into two groups, among which the rats in the experiment group were given intraperitoneal injection of salinomycin, while the rats in the control group were given intraperitoneal injection of normal saline. The change of tumor cells in the two groups was observed. Transwell was used to detect the cell migration and invasion abilities, Real-time PCR was used to detect the expression of m RNA, while Western-blot was utilized for the determination of the expressions of E-cadherin and vimentin proteins. Results: The metastasis and invasion abilities of serum bladder cancer cell line T24 after salinomycin treatment in the experiment group were significantly reduced when compared with those in the control group, and the tumor metastasis lesions were decreased from an average of 1.59 to 0.6(P<0.05). T24 cell proliferation in the experiment group was gradually decreasing. T24 cell proliferation at 48 h was significantly lower than that at 12 h and 24 h(P<0.05). T24 cell proliferation at 24 h was significantly lower than that at 12 h(P<0.05). T24 cell proliferation at each timing point in the experiment group was significantly lower than that in the control group(P<0.05). The serum m RNA level and E-cadherin expression in the tumor tissues in the experiment group were significantly higher than those in the control group, while vimentin expression level was significantly lower than that in the control group(P<0.05). Conclusions: Salinomycin can suppress the metastasis and invasion of bladder cancer cells, of which the mechanism is probably associated with the inhibition of EMT of tumor cells.展开更多
Copy number variations(CNVs), which can affect the role of long non-coding RNAs(lncRNAs), are important genetic changes seen in some malignant tumors. We analyzed lncRNAs with CNV to explore the relationship between l...Copy number variations(CNVs), which can affect the role of long non-coding RNAs(lncRNAs), are important genetic changes seen in some malignant tumors. We analyzed lncRNAs with CNV to explore the relationship between lncRNAs and prognosis in bladder cancer(BLCA). Messenger RNA(mRNA) expression levels, DNA methylation, and DNA copy number data of 408 BLCA patients were subjected to integrative bioinformatics analysis. Cluster analysis was performed to obtain different subtypes and differently expressed lncRNAs and coding genes. Weighted gene co-expression network analysis(WGCNA) was performed to identify the co-expression gene and lncRNA modules. CNV-associated lncRNA data and their influence on cancer prognosis were assessed with Kaplan-Meier survival curve. Multi-omics integration analysis revealed five prognostic lncRNAs with CNV, namely NR2 F1-AS1, LINC01138, THUMPD3-AS1, LOC101928489, and TMEM147-AS1, and a risk-score signature related to overall survival in BLCA was identified. Moreover, validated results in another independent Gene Expression Omnibus(GEO) dataset, GSE31684, were consistent with these results. Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis revealed that the mitogen-activated protein kinase(MAPK) signaling pathway, focal adhesion pathway, and Janus kinase-signal transducers and activators of transcription(JAK-STAT) signaling pathway were enriched in a high-risk score pattern, suggesting that imbalance in these pathways is closely related to tumor development. We revealed the prognosis-related lncRNAs by analyzing the expression profiles of lncRNAs and CNVs, which can be used as prognostic biomarkers for BLCA.展开更多
Background:Bipolar transurethral enucleation of the prostate(B-TUEP)is a well-established surgical treatment for benign prostatic hyperplasia(BPH);however,its efficacy may vary depending on patient characteristics.Mag...Background:Bipolar transurethral enucleation of the prostate(B-TUEP)is a well-established surgical treatment for benign prostatic hyperplasia(BPH);however,its efficacy may vary depending on patient characteristics.Magnetic resonance imaging(MRI)with radiomics analysis can offer comprehensive and quantitative information about prostate characteristics that may relate to surgical outcomes.This study aimed to explore the value of MRI and radiomics analysis in predicting the short-term efficacy of B-TUEP for BPH.Materials and methods:A total of 137 patients with BPH who underwent B-TUEP at 2 institutions were included.Radiological features were measured in the MRIs,and the radiomics score was developed from 1702 radiomics features extracted from the prostate and transitional zone regions of interest.Three prediction models were developed and validated based on clinical-radiological features,radiomic features,and their combinations.The models were evaluated using the area under the receiver operating characteristic curve,calibration curve,and decision curve analysis.Results:The combination model exhibited the highest area under curve in both the training set(0.838)and the external validation set(0.802),indicating superior predictive performance and robustness.Furthermore,the combination model demonstrated good calibration(p>0.05)and optimal clinical utility.The combination model indicated that a highermaximum urine flow rate,lower transitional zone index,and higher radiomics score were associated with an increased risk of poor efficacy.Conclusions:Magnetic resonance imaging with radiomic analysis can offer valuable insights for predicting the short-term efficacy of BTUEP in patients with BPH.A combination model based on clinical and radiomics features can assist urologists in making more precise clinical decisions.展开更多
文摘Objective: To explore the effect of salinomycin on the metastasis and invasion of bladder cancer cell line T24 by regulating the related protein expression in the process of epithelialmesenchymal transition(EMT), and to provide experimental basis for the treatment of urological tumors. Methods: The bladder cancer cell line T24 was cultured in vitro. The rat bladder tumor model was established in vivo. The rats were randomized into two groups, among which the rats in the experiment group were given intraperitoneal injection of salinomycin, while the rats in the control group were given intraperitoneal injection of normal saline. The change of tumor cells in the two groups was observed. Transwell was used to detect the cell migration and invasion abilities, Real-time PCR was used to detect the expression of m RNA, while Western-blot was utilized for the determination of the expressions of E-cadherin and vimentin proteins. Results: The metastasis and invasion abilities of serum bladder cancer cell line T24 after salinomycin treatment in the experiment group were significantly reduced when compared with those in the control group, and the tumor metastasis lesions were decreased from an average of 1.59 to 0.6(P<0.05). T24 cell proliferation in the experiment group was gradually decreasing. T24 cell proliferation at 48 h was significantly lower than that at 12 h and 24 h(P<0.05). T24 cell proliferation at 24 h was significantly lower than that at 12 h(P<0.05). T24 cell proliferation at each timing point in the experiment group was significantly lower than that in the control group(P<0.05). The serum m RNA level and E-cadherin expression in the tumor tissues in the experiment group were significantly higher than those in the control group, while vimentin expression level was significantly lower than that in the control group(P<0.05). Conclusions: Salinomycin can suppress the metastasis and invasion of bladder cancer cells, of which the mechanism is probably associated with the inhibition of EMT of tumor cells.
基金supported by the Natural Science Foundation of Guangdong Province (No. 2017A030313898)the Guangzhou Science and Technology Plan Projects (No. 201707010113), China。
文摘Copy number variations(CNVs), which can affect the role of long non-coding RNAs(lncRNAs), are important genetic changes seen in some malignant tumors. We analyzed lncRNAs with CNV to explore the relationship between lncRNAs and prognosis in bladder cancer(BLCA). Messenger RNA(mRNA) expression levels, DNA methylation, and DNA copy number data of 408 BLCA patients were subjected to integrative bioinformatics analysis. Cluster analysis was performed to obtain different subtypes and differently expressed lncRNAs and coding genes. Weighted gene co-expression network analysis(WGCNA) was performed to identify the co-expression gene and lncRNA modules. CNV-associated lncRNA data and their influence on cancer prognosis were assessed with Kaplan-Meier survival curve. Multi-omics integration analysis revealed five prognostic lncRNAs with CNV, namely NR2 F1-AS1, LINC01138, THUMPD3-AS1, LOC101928489, and TMEM147-AS1, and a risk-score signature related to overall survival in BLCA was identified. Moreover, validated results in another independent Gene Expression Omnibus(GEO) dataset, GSE31684, were consistent with these results. Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis revealed that the mitogen-activated protein kinase(MAPK) signaling pathway, focal adhesion pathway, and Janus kinase-signal transducers and activators of transcription(JAK-STAT) signaling pathway were enriched in a high-risk score pattern, suggesting that imbalance in these pathways is closely related to tumor development. We revealed the prognosis-related lncRNAs by analyzing the expression profiles of lncRNAs and CNVs, which can be used as prognostic biomarkers for BLCA.
基金supported by a grant from the Basic and Applied Basic Research Foundation of Guangdong Province,China(grant no.2019A1515010386).
文摘Background:Bipolar transurethral enucleation of the prostate(B-TUEP)is a well-established surgical treatment for benign prostatic hyperplasia(BPH);however,its efficacy may vary depending on patient characteristics.Magnetic resonance imaging(MRI)with radiomics analysis can offer comprehensive and quantitative information about prostate characteristics that may relate to surgical outcomes.This study aimed to explore the value of MRI and radiomics analysis in predicting the short-term efficacy of B-TUEP for BPH.Materials and methods:A total of 137 patients with BPH who underwent B-TUEP at 2 institutions were included.Radiological features were measured in the MRIs,and the radiomics score was developed from 1702 radiomics features extracted from the prostate and transitional zone regions of interest.Three prediction models were developed and validated based on clinical-radiological features,radiomic features,and their combinations.The models were evaluated using the area under the receiver operating characteristic curve,calibration curve,and decision curve analysis.Results:The combination model exhibited the highest area under curve in both the training set(0.838)and the external validation set(0.802),indicating superior predictive performance and robustness.Furthermore,the combination model demonstrated good calibration(p>0.05)and optimal clinical utility.The combination model indicated that a highermaximum urine flow rate,lower transitional zone index,and higher radiomics score were associated with an increased risk of poor efficacy.Conclusions:Magnetic resonance imaging with radiomic analysis can offer valuable insights for predicting the short-term efficacy of BTUEP in patients with BPH.A combination model based on clinical and radiomics features can assist urologists in making more precise clinical decisions.
