This study is based on the background of the new era of innovation and inheritance in traditional Chinese medicine(TCM),with the fundamental goal of promoting the high-quality development of TCM.Guided by a deep under...This study is based on the background of the new era of innovation and inheritance in traditional Chinese medicine(TCM),with the fundamental goal of promoting the high-quality development of TCM.Guided by a deep understanding of General Secretary Xi Jinping's important discussions on TCM work,the study systematically investigates and analyzes the complementary advantages and collaborative innovation potential of apprenticeship education and university education.It focuses on the core proposition of‘dual-track integration‘,systematically explaining the internal mechanisms and pathways for the compatible development of the two educational models at both theoretical and practical levels.The research aims to provide theoretical support and practical reference for constructing a new mechanism for TCM talent training that meets the needs of the times。展开更多
Under the current medical education reform,the“clinical-research”model for TCM master’s degrees is a key approach to advancing the modernization of traditional Chinese medicine.With the core of“dual-track integrat...Under the current medical education reform,the“clinical-research”model for TCM master’s degrees is a key approach to advancing the modernization of traditional Chinese medicine.With the core of“dual-track integration,”this model aims to enhance both clinical practice and research abilities simultaneously.However,ten years of practice have shown that it still faces multiple challenges:an imbalance between clinical rotation time and research investment,deeply rooted attitudes that prioritize clinical work over research,insufficient TCM research resources and fragmented platforms,and poor coordination between policy and teaching design.These issues,particularly the methodological differences between TCM experience-based medicine and modern evidence-based medicine,further complicate the integration of clinical and research efforts.Therefore,there is an urgent need to promote the deep integration of research training into clinical practice through system design,value orientation,and evaluation systems,fostering a new ecological environment where clinical and research efforts thrive together.This will help cultivate TCM professionals with both strong clinical skills and innovative research capabilities,providing sustained momentum for the high-quality development of traditional Chinese medicine.展开更多
This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targ...This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.展开更多
Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to ev...Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to evaluate the relationship between NER gene polymorphisms and the risk of hepatoblastoma in Eastern Chinese Han children.Methods:In this five-center case-control study,we enrolled 966 subjects from East China(193 hepatoblastoma patients and 773 healthy controls).The TaqMan method was used to genotype 19 single nucleotide polymorphisms(SNPs)in NER pathway genes,including ERCC1,XPA,XPC,XPD,XPF,and XPG.Then,multivariate logistic regression analysis was performed,and odds ratios(ORs)and 95%confidence intervals(95%CIs)were utilized to assess the strength of associations.Results:Three SNPs were related to hepatoblastoma risk.XPC rs2229090 and XPD rs3810366 significantly contributed to hepatoblastoma risk according to the dominant model(adjusted OR=1.49,95%CI=1.07−2.08,P=0.019;adjusted OR=1.66,95%CI=1.12−2.45,P=0.012,respectively).However,XPD rs238406 conferred a significantly decreased risk of hepatoblastoma under the dominant model(adjusted OR=0.68,95%CI=0.49−0.95;P=0.024).Stratified analysis demonstrated that these significant associations were more prominent in certain subgroups.Moreover,there was evidence of functional implications of these significant SNPs suggested by online expression quantitative trait loci(eQTLs)and splicing quantitative trait loci(sQTLs)analysis.Conclusions:In summary,NER pathway gene polymorphisms(XPC rs2229090,XPD rs3810366,and XPD rs238406)are significantly associated with hepatoblastoma risk,and further research is required to verify these findings.展开更多
Dear Editor,Neuroblastoma is the most common non-central nerve system(CNS)solid tumor in pediatrics[1].Neuroblastoma accounts for approximately 8%of all pediatric cancers but disproportionally causes a high cancer mor...Dear Editor,Neuroblastoma is the most common non-central nerve system(CNS)solid tumor in pediatrics[1].Neuroblastoma accounts for approximately 8%of all pediatric cancers but disproportionally causes a high cancer mortality(15%)in children[2].Pediatric patients with low-risk neuroblastoma witness a 5-year overall survival rate>90%,whereas the 5-year overall survival rate in high-risk neuroblastoma pediatric patients is<40%[3].展开更多
基金Yunnan Province High-level Science and Technology Talents and Innovation Team Selection Special Project(Project No.:202305AS350007)。
文摘This study is based on the background of the new era of innovation and inheritance in traditional Chinese medicine(TCM),with the fundamental goal of promoting the high-quality development of TCM.Guided by a deep understanding of General Secretary Xi Jinping's important discussions on TCM work,the study systematically investigates and analyzes the complementary advantages and collaborative innovation potential of apprenticeship education and university education.It focuses on the core proposition of‘dual-track integration‘,systematically explaining the internal mechanisms and pathways for the compatible development of the two educational models at both theoretical and practical levels.The research aims to provide theoretical support and practical reference for constructing a new mechanism for TCM talent training that meets the needs of the times。
基金Yunnan Province High-level Science and Technology Talents and Innovation Team Selection Special Project。
文摘Under the current medical education reform,the“clinical-research”model for TCM master’s degrees is a key approach to advancing the modernization of traditional Chinese medicine.With the core of“dual-track integration,”this model aims to enhance both clinical practice and research abilities simultaneously.However,ten years of practice have shown that it still faces multiple challenges:an imbalance between clinical rotation time and research investment,deeply rooted attitudes that prioritize clinical work over research,insufficient TCM research resources and fragmented platforms,and poor coordination between policy and teaching design.These issues,particularly the methodological differences between TCM experience-based medicine and modern evidence-based medicine,further complicate the integration of clinical and research efforts.Therefore,there is an urgent need to promote the deep integration of research training into clinical practice through system design,value orientation,and evaluation systems,fostering a new ecological environment where clinical and research efforts thrive together.This will help cultivate TCM professionals with both strong clinical skills and innovative research capabilities,providing sustained momentum for the high-quality development of traditional Chinese medicine.
基金supported by the National Natural Science Foundation of China,No.81272986the Natural Science Foundation of Shandong Province,No.ZR2011HZ002
文摘This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
基金supported by grants from the Innovation and Cultivation Fund Project of the Seventh Medical Center,PLA General Hospital(No.QZX-2023-7)Postdoctoral Science Foundation of China(No.2021M691649)Postdoctoral Science Foundation of Jiangsu Province(No.2021K524C).
文摘Objective:Nucleotide excision repair(NER)plays a vital role in maintaining genome stability,and the effect of NER gene polymorphisms on hepatoblastoma susceptibility is still under investigation.This study aimed to evaluate the relationship between NER gene polymorphisms and the risk of hepatoblastoma in Eastern Chinese Han children.Methods:In this five-center case-control study,we enrolled 966 subjects from East China(193 hepatoblastoma patients and 773 healthy controls).The TaqMan method was used to genotype 19 single nucleotide polymorphisms(SNPs)in NER pathway genes,including ERCC1,XPA,XPC,XPD,XPF,and XPG.Then,multivariate logistic regression analysis was performed,and odds ratios(ORs)and 95%confidence intervals(95%CIs)were utilized to assess the strength of associations.Results:Three SNPs were related to hepatoblastoma risk.XPC rs2229090 and XPD rs3810366 significantly contributed to hepatoblastoma risk according to the dominant model(adjusted OR=1.49,95%CI=1.07−2.08,P=0.019;adjusted OR=1.66,95%CI=1.12−2.45,P=0.012,respectively).However,XPD rs238406 conferred a significantly decreased risk of hepatoblastoma under the dominant model(adjusted OR=0.68,95%CI=0.49−0.95;P=0.024).Stratified analysis demonstrated that these significant associations were more prominent in certain subgroups.Moreover,there was evidence of functional implications of these significant SNPs suggested by online expression quantitative trait loci(eQTLs)and splicing quantitative trait loci(sQTLs)analysis.Conclusions:In summary,NER pathway gene polymorphisms(XPC rs2229090,XPD rs3810366,and XPD rs238406)are significantly associated with hepatoblastoma risk,and further research is required to verify these findings.
基金This work was supported by the grants from Natural Science Foundation of Guangdong Province(2019A1515010^(3)60)Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease(2019B030301004).
文摘Dear Editor,Neuroblastoma is the most common non-central nerve system(CNS)solid tumor in pediatrics[1].Neuroblastoma accounts for approximately 8%of all pediatric cancers but disproportionally causes a high cancer mortality(15%)in children[2].Pediatric patients with low-risk neuroblastoma witness a 5-year overall survival rate>90%,whereas the 5-year overall survival rate in high-risk neuroblastoma pediatric patients is<40%[3].
