As a vectorial property,polarization encodes high-dimensional information of light.Polarization-based imaging can characterize detailed structural features of biomedical samples label-freely.However,compared with othe...As a vectorial property,polarization encodes high-dimensional information of light.Polarization-based imaging can characterize detailed structural features of biomedical samples label-freely.However,compared with other fundamental properties of light,such as intensity,wavelength and phase,polarization has a shorter application history in biomedicine,because of the requirement for both advanced polarization optical components and computational approaches,which can be achieved nowadays with the fast theoretical and hardware development.展开更多
Polarimctry is a powerful optical tool in thc biomcdical ficld,providing morc comprchensive information on thc sub-wavclcngth micro-physical structurc of a samplc than traditional light intensity mcasurement techniquc...Polarimctry is a powerful optical tool in thc biomcdical ficld,providing morc comprchensive information on thc sub-wavclcngth micro-physical structurc of a samplc than traditional light intensity mcasurement techniqucs.This revicw summarizcs thc concepts and tcchniqucs of polarization and its biomedical applications.Spccifically,we first bricfly describc thc basic principles of polarizcd light and thc Mucller matrix(MM)decomposition method,followed by some rcscarch progress of polarimctric mcasurement techniqucs in recent ycars.Finally,wc introducc somc studics on biological tissucs and cells,and then illustrate thc application valuc of polarization optical mcthod.展开更多
Asan emerging poserful tool to provide structural informstion af tissue specimens label-freely,Mueller matrix(MM)polarimetry has garnered extensive attention in biomedical studies and pathological diagnois.However,for...Asan emerging poserful tool to provide structural informstion af tissue specimens label-freely,Mueller matrix(MM)polarimetry has garnered extensive attention in biomedical studies and pathological diagnois.However,for the commonly used constant-step rotating MM polarimetricsystem,beam drift induæd by the rotation of polarization eements can lead to distortions in messurement results,severely affect ing MM imaging accuracy.Here,based on our previous study,we prоровe an optimizad self-registration method to mitigate the psæudo-depolarization effects introduced by image artifacts in constant-step rotatin g MM polarimetry.By addresing the prevalent issue of beam drift and image distortions in such polarimetric imaging systems,the effectivenes of the proposed method is experimentally validated using tissue samples.The result.s demonstrate a significant enhanæment in the accuIrsсy of depolarization parameter estimation after applying the optimized self-registration method.Furthermore,the method enhances the coarseness and contrsst of MM-derived parameters images,thereby bolstering their capacity to characterize tissuestructures.The optimized self-registration method proposed in this study can provide an innovstive spproach for quantitative tissue polarimetry bssæd on constant-step ro tating MM messurement,and contribute to the advanæment of polarimetric imaging technology in biomedical applications.展开更多
Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a n...Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.展开更多
It is of great significance in clinical diagnosis and treatment evaluation to accurately identify the lesion tissue and further extract its characteristics and depth location. In this study, we construct tissue phanto...It is of great significance in clinical diagnosis and treatment evaluation to accurately identify the lesion tissue and further extract its characteristics and depth location. In this study, we construct tissue phantoms for three lesion types: fibrosis (FT), organelle proliferation (OPT), and pigmentation (PT). These phantoms allow for the quantitative regulation of mimicked disease depth. The experimental results show that the parameter Kc, combined with MMT parameters, can effectively distinguish the presence of lesions and their abnormal types. Further, the study extracts depth-sensitive polarization feature parameters (DSPFPs) for specific lesion types. Through experiments of tissue phantoms with various depth settings, the established machine learning regression models based on DSPFPs demonstrate their depth retrieval capabilities.展开更多
Mueller matrix polarimetry(MMP)has been proven to be a powerful tool for characterizing the microstructural features of biological samples in biomedical research and clinical diagnostics.However,the traditional Muelle...Mueller matrix polarimetry(MMP)has been proven to be a powerful tool for characterizing the microstructural features of biological samples in biomedical research and clinical diagnostics.However,the traditional Mueller matrix(MM)imaging technique based on single exposure has a limited dynamic range,leading to poor polarization image quality for biological samples with signi-cant contrast variations.In this study,we propose a novel method to generate high dynamic range(HDR)MM images based on a multi-exposure fusion algorithm.By employing an optimal exposure selection strategy for transmission imaging and a multi-exposure weighted averaging strategy for backscattering imaging,the method expands the dynamic range while accurately preserving the polarization information of the samples.Experiments of sliced and bulk tissues demonstrate that the proposed method signi¯cantly suppresses the scattering noise and improves the quality of extracted polarization parameter images,especially in accurate distinction of di®erent pathological areas.These results highlight the potential of HDR MM imaging technology in extracting polarization information from complex biological samples with high resolution and contrast.展开更多
We developed a model to describe polarized photon scattering in biological tissues.In this model,tissues are simplified to a mixture of scatterers and surrounding mpdium.There are t wo types ofscatterers in the model:...We developed a model to describe polarized photon scattering in biological tissues.In this model,tissues are simplified to a mixture of scatterers and surrounding mpdium.There are t wo types ofscatterers in the model:solid spheres and infinitely long solid cylinders.Variables related to thescatterers include:the densities and sizes of the spheres and cylinders,the orientation and angulardistribution of cylinders.Variables related to the surrounding medium include:the refractiveindex,absorption coeficient and birefringence.In this paper,as a development we introduce anoptical activity effct to the model.By comparing experiments and Monte Carlo simulations,we analyze the backscater ing Mueller matrix patterms of several tissue-like media,and summarizethe different effects coming from anisotropic scattering and optical properties.In addition,wepropose a possible method to extract the optical activity values for tissues.Both the experimentaland simulated results show that,by analyzing the Mueller matrix patterns,the microstructureand optical properties of the medium can be obtained.The characteristic features of Muellermatrix patterns are potentilly powerful tools for studying the contrast mechanisms of polari-zation imaging for medical diagnosis.展开更多
We propose and conduct both the rotating linear polarization imaging(RLPI)and Mueller matrix transformation(MMT)measurements of different biological tissue samples,and testify the capability of the Mueller matrix pola...We propose and conduct both the rotating linear polarization imaging(RLPI)and Mueller matrix transformation(MMT)measurements of different biological tissue samples,and testify the capability of the Mueller matrix polarimetry for the anisotropic scattering media.The independent parameters extracted from the RLPI and MMT techniques are compared and analyzed.The tissue experimental results show that the parameters are closely related to the structural characteristics of the turbid scattering media,including the sizes of the scatterers,the angular distribution and order of alignment of thefibers.The results and conclusions in this paper may provide a potential method for the detection of precancerous and early stage cancerous tissues.Also,such studies represent the Mueller matrix transformation procedure which results in a set of parameters linking up the Mueller matrix elements to the structural and optical properties of the media.展开更多
Intervertebral disc degeneration(IVDD)can be caused by aging,injury,and genetic factors.The pathological changes associated with IVDD include the excessive accumulation of reactive oxygen species(ROS),cellular pyropto...Intervertebral disc degeneration(IVDD)can be caused by aging,injury,and genetic factors.The pathological changes associated with IVDD include the excessive accumulation of reactive oxygen species(ROS),cellular pyroptosis,and extracellular matrix(ECM)degradation.There are currently no approved specific molecular therapies for IVDD.In this study,we developed a multifunctional and microenvironment-responsive metal-phenolic network release platform,termed TMP@Alg-PBA/PVA,which could treat(IL-1β)-induced IVDD.The metal-phenolic network(TA-Mn-PVP,TMP)released from this platform targeted mitochondria to efficiently scavenge ROS and reduce ECM degradation.Pyroptosis was suppressed through the inhibition of the IL-17/ERK signaling pathway.These findings demonstrate the versatility of the platform.And in a rat model of IVDD,TMP@Alg-PBA/PVA exhibited excellent therapeutic effects by reducing the progression of the disease.TMP@Alg-PBA/PVA,therefore,presents clinical potential for the treatment of IVDD.展开更多
The contrast mechanism of different polarization imaging techniques for melanoma in mouse skin is st udied using both experiments and Monte Carlo simulations.Total intensity,linear polariz-ation diference imaging(DPI)...The contrast mechanism of different polarization imaging techniques for melanoma in mouse skin is st udied using both experiments and Monte Carlo simulations.Total intensity,linear polariz-ation diference imaging(DPI),degree of polarization imaging(DOPI)and'rotating linearpolarization imaging(RLPl)are applied and the relative contrasts of these polarization imagingmethods between the normal and cancerous tissues are compared.A two-layer absorption-scat-tering model is proposed to explain the contrast mechanism of the polarization imaging formelanoma.By taking into account of both scattering of symmetrical and asymmetrical scat terersand absorption of inter-scatterer medium,the two-layer model reproduces the relative con trastsfor polarization images observed in experiments.The simulation results also show that,theparameters of polarization imaging change more dramatically with the variation of absorption inthe bottom layer than the top layer.展开更多
We apply diferent polarization imaging techniques for cancerous liver tissues,and compare the relative contrasts for difference polarization imaging(DPI),degree of polarization imaging(DOPI)and rotating linear polariz...We apply diferent polarization imaging techniques for cancerous liver tissues,and compare the relative contrasts for difference polarization imaging(DPI),degree of polarization imaging(DOPI)and rotating linear polarization imaging(RLPI).Experimental results show that a number of polarization imaging parameters are capable of differentiating cancerous cells in isotropic liver tisues.To analyze the contrast mechanism of the cancer:-sensitive polarization imaging parameters,we propose a scattering model cont aining two types of spherical scatterers and carry on Monte Carlo simula tions based on this bi-component model.Both the experimental and Monte Carlo simulated results show that the RLPI technique can provide a good imaging contrast of cancerous tissues.The bi-component scattering model provides a useful tool to ana-lyze the contrast mechanism of polarization imaging of cancerous tissues.展开更多
Achilles tendon injuries,as a widely existing disease,have attracted a lot of research interest.Mueller matrix polarimetry,as a novel label-free quantitative imaging method,has been widely used in various applications...Achilles tendon injuries,as a widely existing disease,have attracted a lot of research interest.Mueller matrix polarimetry,as a novel label-free quantitative imaging method,has been widely used in various applications of lesion identification and pathological diagnosis.However,focusing on the recovery process of Achilles tendon injuries,current optical imaging methods have not yet achieved the label-free precise identification and quantitative evaluation.In this study,using Mueller matrix polarimetry,various Achilles tendon injury samples were characterized specifically,and the efficacy of different recovery schemes was evaluated accordingly.Experiments indicate that injured Achilles tendons show less phase retardance,larger diattenuation,and relatively disordered orientation.The combination of experiments with Monte Carlo simulation results illustrate the microscopic mechanism of the Achilles tendon recovery process from three aspects,that is,the increased fiber diameter,a more consistent fiber orientation,and greater birefringence induced by more collagen protein.Finally,based on the statistical distribution of polarization measurements,a polarization specific characterization parameter was extracted to construct a label-free image,which cannot only intuitively show the injury and recovery of Achilles tendon samples,but also give a quantitative evaluation of the treatment.展开更多
Adaptive optics(AO)is a powerful tool employed across various research fields,from aerospace to microscopy.Traditionally,AO has focused on correcting optical phase aberrations,with recent advances extending to polaris...Adaptive optics(AO)is a powerful tool employed across various research fields,from aerospace to microscopy.Traditionally,AO has focused on correcting optical phase aberrations,with recent advances extending to polarisation compensation.However,intensity errors are also prevalent in optical systems,yet effective correction methods are still in their infancy.Here,we introduce a novel AO approach,termed intensity adaptive optics(I-AO),which employs a dual-feedback loop mechanism to first address non-uniform intensity distribution and subsequently compensate for energy loss at the pupil plane.We demonstrate that I-AO can operate in both sensor-based and sensorless formats and validate its feasibility by quantitatively analysing the focus quality of an aberrated system.This technique expands the AO toolkit,paving the way for next-generation AO technology.展开更多
Advances in vectorial polarization-resolved imaging are bringing new capabilities to applications ranging from fundamental physics through to clinical diagnosis.Imaging polarimetry requires determination of the Muelle...Advances in vectorial polarization-resolved imaging are bringing new capabilities to applications ranging from fundamental physics through to clinical diagnosis.Imaging polarimetry requires determination of the Mueller matrix(MM)at every point,providing a complete description of an object’s vectorial properties.Despite forming a comprehensive representation,the MM does not usually provide easily interpretable information about the object’s internal structure.Certain simpler vectorial metrics are derived from subsets of the MM elements.These metrics permit extraction of signatures that provide direct indicators of hidden optical properties of complex systems,while featuring an intriguing asymmetry about what information can or cannot be inferred via these metrics.We harness such characteristics to reveal the spin Hall effect of light,infer microscopic structure within laser-written photonic waveguides,and conduct rapid pathological diagnosis through analysis of healthy and cancerous tissue.This provides new insight for the broader usage of such asymmetric inferred vectorial information.展开更多
A Mueller matrix covers all the polarization information of the measured sample,however the combination of its 16 elements is sometimes not intuitive enough to describe and identify the key characteristics of polariza...A Mueller matrix covers all the polarization information of the measured sample,however the combination of its 16 elements is sometimes not intuitive enough to describe and identify the key characteristics of polarization changes.Within the Poincarésphere system,this study achieves a spatial representation of the Mueller matrix:the Global-Polarization Stokes Ellipsoid(GPSE).With the help of Monte Carlo simulations combined with anisotropic tissue models,three basic characteristic parameters of GPSE are proposed and explained,where the V parameter represents polarization maintenance ability,and the E and D†parameters represent the degree of anisotropy.Furthermore,based on GPSE system,a dynamic analysis of skeletal muscle dehydration process demonstrates the monitoring efect of GPSE from an application perspective,while confrming its robustness and accuracy.展开更多
Optical Skyrmions have many important properties that make them ideal units for high-density data applications,including the ability to carry digital information through a discrete topological number and the independe...Optical Skyrmions have many important properties that make them ideal units for high-density data applications,including the ability to carry digital information through a discrete topological number and the independence of spatially varying polarization to other dimensions.More importantly,the topological nature of the optical Skyrmion heuristically suggests a strong degree of robustness to perturbations,which is crucial for reliably carrying information in noisy environments.However,the study of the topological robustness of optical Skyrmions is still in its infancy.Here,we quantify this robustness precisely by proving that the topological nature of the Skyrmion arises from its structure on the boundary and,by duality,is resilient to spatially varying perturbations provided they respect the relevant boundary conditions of the unperturbed Skyrmion.We then present experimental evidence validating this robustness in the context of paraxial Skyrmion beams against complex polarization aberrations.Our work provides a framework for handling various perturbations of Skyrmion fields and offers guarantees of robustness in a general sense.This,in turn,has implications for applications of the Skyrmion where their topological nature is exploited explicitly,and,in particular,provides an underpinning for the use of optical Skyrmions in communications and computing.展开更多
Many polarisation techniques have been harnessed for decades in biological and clinical research,each based upon measurement of the vectorial properties of light or the vectorial transformations imposed on light by ob...Many polarisation techniques have been harnessed for decades in biological and clinical research,each based upon measurement of the vectorial properties of light or the vectorial transformations imposed on light by objects.Various advanced vector measurement/sensing techniques,physical interpretation methods,and approaches to analyse biomedically relevant information have been developed and harnessed.In this review,we focus mainly on summarising methodologies and applications related to tissue polarimetry,with an emphasis on the adoption of the Stokes-Mueller formalism.Several recent breakthroughs,development trends,and potential multimodal uses in conjunction with other techniques are also presented.The primary goal of the review is to give the reader a general overview in the use of vectorial information that can be obtained by polarisation optics for applications in biomedical and clinical research.展开更多
文摘As a vectorial property,polarization encodes high-dimensional information of light.Polarization-based imaging can characterize detailed structural features of biomedical samples label-freely.However,compared with other fundamental properties of light,such as intensity,wavelength and phase,polarization has a shorter application history in biomedicine,because of the requirement for both advanced polarization optical components and computational approaches,which can be achieved nowadays with the fast theoretical and hardware development.
基金supported by the Cross-research innovation fund of International Graduate School at Shenzhen,Tsinghua University(JC2021002)Science and Technology Research Program of Shenzhen(JCYJ20200109142820687).
文摘Polarimctry is a powerful optical tool in thc biomcdical ficld,providing morc comprchensive information on thc sub-wavclcngth micro-physical structurc of a samplc than traditional light intensity mcasurement techniqucs.This revicw summarizcs thc concepts and tcchniqucs of polarization and its biomedical applications.Spccifically,we first bricfly describc thc basic principles of polarizcd light and thc Mucller matrix(MM)decomposition method,followed by some rcscarch progress of polarimctric mcasurement techniqucs in recent ycars.Finally,wc introducc somc studics on biological tissucs and cells,and then illustrate thc application valuc of polarization optical mcthod.
基金funded by the National Natural Science Foundation of China(NSFC)under Grant No.62335007.
文摘Asan emerging poserful tool to provide structural informstion af tissue specimens label-freely,Mueller matrix(MM)polarimetry has garnered extensive attention in biomedical studies and pathological diagnois.However,for the commonly used constant-step rotating MM polarimetricsystem,beam drift induæd by the rotation of polarization eements can lead to distortions in messurement results,severely affect ing MM imaging accuracy.Here,based on our previous study,we prоровe an optimizad self-registration method to mitigate the psæudo-depolarization effects introduced by image artifacts in constant-step rotatin g MM polarimetry.By addresing the prevalent issue of beam drift and image distortions in such polarimetric imaging systems,the effectivenes of the proposed method is experimentally validated using tissue samples.The result.s demonstrate a significant enhanæment in the accuIrsсy of depolarization parameter estimation after applying the optimized self-registration method.Furthermore,the method enhances the coarseness and contrsst of MM-derived parameters images,thereby bolstering their capacity to characterize tissuestructures.The optimized self-registration method proposed in this study can provide an innovstive spproach for quantitative tissue polarimetry bssæd on constant-step ro tating MM messurement,and contribute to the advanæment of polarimetric imaging technology in biomedical applications.
基金Shenzhen Key Fundamental Research Project(No.JCYJ20210324120012035).
文摘Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.
基金supported by the Science and Technology Research Program of Shenzhen(No.JCYJ20200109142820687 and JCYJ20210324120012035)the Cross-research Innovation Fund of In-ternational Graduate School at Shenzhen,Tsinghua University(No.JC2021001)the Russian Sci-ence Foundation(No.23-14-00287)。
文摘It is of great significance in clinical diagnosis and treatment evaluation to accurately identify the lesion tissue and further extract its characteristics and depth location. In this study, we construct tissue phantoms for three lesion types: fibrosis (FT), organelle proliferation (OPT), and pigmentation (PT). These phantoms allow for the quantitative regulation of mimicked disease depth. The experimental results show that the parameter Kc, combined with MMT parameters, can effectively distinguish the presence of lesions and their abnormal types. Further, the study extracts depth-sensitive polarization feature parameters (DSPFPs) for specific lesion types. Through experiments of tissue phantoms with various depth settings, the established machine learning regression models based on DSPFPs demonstrate their depth retrieval capabilities.
基金supported by the Cross-research Innovation Fund of the International Graduate School at Shenzhen,Tsinghua University(JC2021002).
文摘Mueller matrix polarimetry(MMP)has been proven to be a powerful tool for characterizing the microstructural features of biological samples in biomedical research and clinical diagnostics.However,the traditional Mueller matrix(MM)imaging technique based on single exposure has a limited dynamic range,leading to poor polarization image quality for biological samples with signi-cant contrast variations.In this study,we propose a novel method to generate high dynamic range(HDR)MM images based on a multi-exposure fusion algorithm.By employing an optimal exposure selection strategy for transmission imaging and a multi-exposure weighted averaging strategy for backscattering imaging,the method expands the dynamic range while accurately preserving the polarization information of the samples.Experiments of sliced and bulk tissues demonstrate that the proposed method signi¯cantly suppresses the scattering noise and improves the quality of extracted polarization parameter images,especially in accurate distinction of di®erent pathological areas.These results highlight the potential of HDR MM imaging technology in extracting polarization information from complex biological samples with high resolution and contrast.
基金supported by National Natural Science China(NSFC)Foundation of Grants No.10974114,11174178,41106034Open Fund of Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes,Jinan University.
文摘We developed a model to describe polarized photon scattering in biological tissues.In this model,tissues are simplified to a mixture of scatterers and surrounding mpdium.There are t wo types ofscatterers in the model:solid spheres and infinitely long solid cylinders.Variables related to thescatterers include:the densities and sizes of the spheres and cylinders,the orientation and angulardistribution of cylinders.Variables related to the surrounding medium include:the refractiveindex,absorption coeficient and birefringence.In this paper,as a development we introduce anoptical activity effct to the model.By comparing experiments and Monte Carlo simulations,we analyze the backscater ing Mueller matrix patterms of several tissue-like media,and summarizethe different effects coming from anisotropic scattering and optical properties.In addition,wepropose a possible method to extract the optical activity values for tissues.Both the experimentaland simulated results show that,by analyzing the Mueller matrix patterns,the microstructureand optical properties of the medium can be obtained.The characteristic features of Muellermatrix patterns are potentilly powerful tools for studying the contrast mechanisms of polari-zation imaging for medical diagnosis.
基金This work was supported by the National Natural Science Foundation of China(NSFC)Grants No.10974114,11174178,41106034.
文摘We propose and conduct both the rotating linear polarization imaging(RLPI)and Mueller matrix transformation(MMT)measurements of different biological tissue samples,and testify the capability of the Mueller matrix polarimetry for the anisotropic scattering media.The independent parameters extracted from the RLPI and MMT techniques are compared and analyzed.The tissue experimental results show that the parameters are closely related to the structural characteristics of the turbid scattering media,including the sizes of the scatterers,the angular distribution and order of alignment of thefibers.The results and conclusions in this paper may provide a potential method for the detection of precancerous and early stage cancerous tissues.Also,such studies represent the Mueller matrix transformation procedure which results in a set of parameters linking up the Mueller matrix elements to the structural and optical properties of the media.
基金supported by the Key Projects of Hunan Provincial Science and Technology Department,China (2021RC4057)Key R&D Program of Hunan Provincial Science and Technology Department,China (2023SK2044)+1 种基金Natural Science Foundation of Hunan Province,China (2023JJ40906)Natural Science Foundation of Changsha,China (kq2208364).
文摘Intervertebral disc degeneration(IVDD)can be caused by aging,injury,and genetic factors.The pathological changes associated with IVDD include the excessive accumulation of reactive oxygen species(ROS),cellular pyroptosis,and extracellular matrix(ECM)degradation.There are currently no approved specific molecular therapies for IVDD.In this study,we developed a multifunctional and microenvironment-responsive metal-phenolic network release platform,termed TMP@Alg-PBA/PVA,which could treat(IL-1β)-induced IVDD.The metal-phenolic network(TA-Mn-PVP,TMP)released from this platform targeted mitochondria to efficiently scavenge ROS and reduce ECM degradation.Pyroptosis was suppressed through the inhibition of the IL-17/ERK signaling pathway.These findings demonstrate the versatility of the platform.And in a rat model of IVDD,TMP@Alg-PBA/PVA exhibited excellent therapeutic effects by reducing the progression of the disease.TMP@Alg-PBA/PVA,therefore,presents clinical potential for the treatment of IVDD.
基金supported by the National Natural Science Foundation of China(NSFC)Grant Nos.10974114,11174178,61205199the Knowledge Innovation Program of Basic Research Projects of Shenzhen Grant No.JCY20130402145002404.
文摘The contrast mechanism of different polarization imaging techniques for melanoma in mouse skin is st udied using both experiments and Monte Carlo simulations.Total intensity,linear polariz-ation diference imaging(DPI),degree of polarization imaging(DOPI)and'rotating linearpolarization imaging(RLPl)are applied and the relative contrasts of these polarization imagingmethods between the normal and cancerous tissues are compared.A two-layer absorption-scat-tering model is proposed to explain the contrast mechanism of the polarization imaging formelanoma.By taking into account of both scattering of symmetrical and asymmetrical scat terersand absorption of inter-scatterer medium,the two-layer model reproduces the relative con trastsfor polarization images observed in experiments.The simulation results also show that,theparameters of polarization imaging change more dramatically with the variation of absorption inthe bottom layer than the top layer.
基金supported by the National Natural Science Foundation of China(NSFC)Grants No.10974114,11174178,41106034Open Fund of Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes,Jinan University.
文摘We apply diferent polarization imaging techniques for cancerous liver tissues,and compare the relative contrasts for difference polarization imaging(DPI),degree of polarization imaging(DOPI)and rotating linear polarization imaging(RLPI).Experimental results show that a number of polarization imaging parameters are capable of differentiating cancerous cells in isotropic liver tisues.To analyze the contrast mechanism of the cancer:-sensitive polarization imaging parameters,we propose a scattering model cont aining two types of spherical scatterers and carry on Monte Carlo simula tions based on this bi-component model.Both the experimental and Monte Carlo simulated results show that the RLPI technique can provide a good imaging contrast of cancerous tissues.The bi-component scattering model provides a useful tool to ana-lyze the contrast mechanism of polarization imaging of cancerous tissues.
文摘Achilles tendon injuries,as a widely existing disease,have attracted a lot of research interest.Mueller matrix polarimetry,as a novel label-free quantitative imaging method,has been widely used in various applications of lesion identification and pathological diagnosis.However,focusing on the recovery process of Achilles tendon injuries,current optical imaging methods have not yet achieved the label-free precise identification and quantitative evaluation.In this study,using Mueller matrix polarimetry,various Achilles tendon injury samples were characterized specifically,and the efficacy of different recovery schemes was evaluated accordingly.Experiments indicate that injured Achilles tendons show less phase retardance,larger diattenuation,and relatively disordered orientation.The combination of experiments with Monte Carlo simulation results illustrate the microscopic mechanism of the Achilles tendon recovery process from three aspects,that is,the increased fiber diameter,a more consistent fiber orientation,and greater birefringence induced by more collagen protein.Finally,based on the statistical distribution of polarization measurements,a polarization specific characterization parameter was extracted to construct a label-free image,which cannot only intuitively show the injury and recovery of Achilles tendon samples,but also give a quantitative evaluation of the treatment.
基金support of Dr.Yun Zhang and Prof.Daniel Royston at the University of Oxford,St John's College(C.H.),and the Royal Society(URF\R1/241734)(C.H.).
文摘Adaptive optics(AO)is a powerful tool employed across various research fields,from aerospace to microscopy.Traditionally,AO has focused on correcting optical phase aberrations,with recent advances extending to polarisation compensation.However,intensity errors are also prevalent in optical systems,yet effective correction methods are still in their infancy.Here,we introduce a novel AO approach,termed intensity adaptive optics(I-AO),which employs a dual-feedback loop mechanism to first address non-uniform intensity distribution and subsequently compensate for energy loss at the pupil plane.We demonstrate that I-AO can operate in both sensor-based and sensorless formats and validate its feasibility by quantitatively analysing the focus quality of an aberrated system.This technique expands the AO toolkit,paving the way for next-generation AO technology.
基金supported by the European Research Council (Ad OMi S, No. 695140) (C. H. and M. J. B.)the Engineering and Physical Sciences Research Council (UK) (No. EP/ R004803/01) (P. S. S.)+2 种基金the National Natural Science Foundation of China (11974206 and 61527826) (H. M.)Shenzhen Fundamental Research and Discipline Layout Project (No. JCYJ20170412170814624) (H. H., M. Z., and H. M.)H2020-MSCAIF-2018 Program under Grant No. 838199 (S. C. T.)
文摘Advances in vectorial polarization-resolved imaging are bringing new capabilities to applications ranging from fundamental physics through to clinical diagnosis.Imaging polarimetry requires determination of the Mueller matrix(MM)at every point,providing a complete description of an object’s vectorial properties.Despite forming a comprehensive representation,the MM does not usually provide easily interpretable information about the object’s internal structure.Certain simpler vectorial metrics are derived from subsets of the MM elements.These metrics permit extraction of signatures that provide direct indicators of hidden optical properties of complex systems,while featuring an intriguing asymmetry about what information can or cannot be inferred via these metrics.We harness such characteristics to reveal the spin Hall effect of light,infer microscopic structure within laser-written photonic waveguides,and conduct rapid pathological diagnosis through analysis of healthy and cancerous tissue.This provides new insight for the broader usage of such asymmetric inferred vectorial information.
基金the Science and Technology Research Program of Shenzhen(Nos.JCYJ20200109142820687 and JCYJ20210324120012035)the Cross-research Innovation Fund of the International Graduate School at Shenzhen,Tsinghua University(No.JC2021002)the Russian Science Foundation(No.23-14-00287).
文摘A Mueller matrix covers all the polarization information of the measured sample,however the combination of its 16 elements is sometimes not intuitive enough to describe and identify the key characteristics of polarization changes.Within the Poincarésphere system,this study achieves a spatial representation of the Mueller matrix:the Global-Polarization Stokes Ellipsoid(GPSE).With the help of Monte Carlo simulations combined with anisotropic tissue models,three basic characteristic parameters of GPSE are proposed and explained,where the V parameter represents polarization maintenance ability,and the E and D†parameters represent the degree of anisotropy.Furthermore,based on GPSE system,a dynamic analysis of skeletal muscle dehydration process demonstrates the monitoring efect of GPSE from an application perspective,while confrming its robustness and accuracy.
基金support of St John’s College,the University of Oxford,and the Royal Society(URF\R1\241734)(C.H.)the European Research Council(AdOMiS,no.695140)(C.H.and M.J.B.)Shenzhen Key Fundamental Research Project(No.JCYJ20210324120012035)(H.H.).
文摘Optical Skyrmions have many important properties that make them ideal units for high-density data applications,including the ability to carry digital information through a discrete topological number and the independence of spatially varying polarization to other dimensions.More importantly,the topological nature of the optical Skyrmion heuristically suggests a strong degree of robustness to perturbations,which is crucial for reliably carrying information in noisy environments.However,the study of the topological robustness of optical Skyrmions is still in its infancy.Here,we quantify this robustness precisely by proving that the topological nature of the Skyrmion arises from its structure on the boundary and,by duality,is resilient to spatially varying perturbations provided they respect the relevant boundary conditions of the unperturbed Skyrmion.We then present experimental evidence validating this robustness in the context of paraxial Skyrmion beams against complex polarization aberrations.Our work provides a framework for handling various perturbations of Skyrmion fields and offers guarantees of robustness in a general sense.This,in turn,has implications for applications of the Skyrmion where their topological nature is exploited explicitly,and,in particular,provides an underpinning for the use of optical Skyrmions in communications and computing.
基金The project was supported by the European Research Council(AdOMiS,no.695140)Shenzhen Fundamental Research and Discipline Layout Project(JCYJ20170412170814624)。
文摘Many polarisation techniques have been harnessed for decades in biological and clinical research,each based upon measurement of the vectorial properties of light or the vectorial transformations imposed on light by objects.Various advanced vector measurement/sensing techniques,physical interpretation methods,and approaches to analyse biomedically relevant information have been developed and harnessed.In this review,we focus mainly on summarising methodologies and applications related to tissue polarimetry,with an emphasis on the adoption of the Stokes-Mueller formalism.Several recent breakthroughs,development trends,and potential multimodal uses in conjunction with other techniques are also presented.The primary goal of the review is to give the reader a general overview in the use of vectorial information that can be obtained by polarisation optics for applications in biomedical and clinical research.
