L-4-Fluorophenylalanine(FPhe)was prepared from 4-fluorophenylpyruvate(FPPA)catalyzed by aspartate aminotransferase(Asp-AT)of the recombinant E.coli BL21-pET/aspC.After 12 h enzymatic reaction,the FPPA conversion was o...L-4-Fluorophenylalanine(FPhe)was prepared from 4-fluorophenylpyruvate(FPPA)catalyzed by aspartate aminotransferase(Asp-AT)of the recombinant E.coli BL21-pET/aspC.After 12 h enzymatic reaction,the FPPA conversion was over 90%and the yield of FPhe could be above 85%under the following optimal conditions:37uC,pH value range of 5.0–8.0,5.5 mass ratio of cell to FPPA,0.6%(w/v)of Tween 80,7.08 g/L FPPA,and 1.6 of molar ratio of L-Asp to FPPA.展开更多
基金the National Natural Science Foundation of China(Grant No.20336010)the State Key Development Program for Basic Research of China(No.2003CB716004).
文摘L-4-Fluorophenylalanine(FPhe)was prepared from 4-fluorophenylpyruvate(FPPA)catalyzed by aspartate aminotransferase(Asp-AT)of the recombinant E.coli BL21-pET/aspC.After 12 h enzymatic reaction,the FPPA conversion was over 90%and the yield of FPhe could be above 85%under the following optimal conditions:37uC,pH value range of 5.0–8.0,5.5 mass ratio of cell to FPPA,0.6%(w/v)of Tween 80,7.08 g/L FPPA,and 1.6 of molar ratio of L-Asp to FPPA.
