A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were w...A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were withering time,fermentation time and fermentation temperature.Each factor was set by three levels.The responses were predicted by analysis of the regression equation.In the study,the models of regression equation were significant by the analysis of variance(ANOVA).The second-order response surface figures were showed that interaction term is correlated with the response.Optimal process parameters for the maximum of TF and TR formation were obtained at withering time of 12 hours,fermentation time of 2 hours,and fermentation temperature of 30℃.The predictions of TF and TR formation were 0.52% and 3.98%.The results obtained in this study showed that the CCD and Response Surface Methodology(RSM) could be an effective tool to optimize the process parameters of Congou black tea manufacture.展开更多
The integration of distributed optical fiber temperature sensor with supervisory control and data acquisition （SCADA） system is proposed and implemented. In the implementation of the integration, both the compatibil...The integration of distributed optical fiber temperature sensor with supervisory control and data acquisition （SCADA） system is proposed and implemented. In the implementation of the integration, both the compatibility with traditional system and the characteristics of distributed optical fiber temperature sensor is considered before Modbus TCP/IP protocol is chosen. The protocol is implemented with open source component Indy. The Modbus TCP/IP protocol used in the system is proved to be fast and robust.展开更多
Background： Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated...Background： Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells （EPCs） derived from human early fetal aortas in rat injured arteries. Methods： The carotid arterial endothelium of Sprague-Dawley rats was damaged by dilatation with a 1.5 F balloon catheter, and then EPCs derived from human early fetal aortas （〈14 weeks） were injected into the lumen of the injured artery in transplanted rats, with an equal volume of normal saline injected into control rats. Rats were sacrificed at 2 and 4 weeks after treatment and transplanted cells were identified by immunohistochemical staining with anti-human CD31 and anti-human mitochondria antibodies. Arterial cross-sections were analyzed by pathology, immunohistochemistry, and morphometry. Results： Green fluorescence-labeled EPCs could be seen in the endovascular surface of balloon-injured vessels after transplantation. The intimal area and intimal/medial area ratio were significantly smaller in the transplanted group than in the control （P 〈 0.05） and the residual lumen area was larger （P 〈 0.05）. After EPC transplantation, a complete vascular endothelial layer was formed, which was positive for human yon Willebrand factor after immunohistochemical staining, and immunohistochemical staining revealed many CD31- and mitochondria-positive cells in the re-endothelialized endothelium with EPC transplantation but not control treatment. Conclusion： EPCs derived from human early fetal aorta were successfully transplanted into injured vessels and might inhibit neointimal hyperplasia after vascular injury.展开更多
基金Supported by the Pillar Program of the Ministry of Science and Technology of China 2012BAF07B05
文摘A central composite design(CCD),with three variables and three levels,was used to optimize withering and fermentation processing during manufacture of Congou black tea of Sichuan.The three independent variables were withering time,fermentation time and fermentation temperature.Each factor was set by three levels.The responses were predicted by analysis of the regression equation.In the study,the models of regression equation were significant by the analysis of variance(ANOVA).The second-order response surface figures were showed that interaction term is correlated with the response.Optimal process parameters for the maximum of TF and TR formation were obtained at withering time of 12 hours,fermentation time of 2 hours,and fermentation temperature of 30℃.The predictions of TF and TR formation were 0.52% and 3.98%.The results obtained in this study showed that the CCD and Response Surface Methodology(RSM) could be an effective tool to optimize the process parameters of Congou black tea manufacture.
基金supported by the National Natural Science Foundation of China under Grant No. 60608009Science Foundation of Zhejiang Province under Grant No. Y107091 and ScienceTechnology Department of Zhejiang Province under Grant No. 2008C21172.
文摘The integration of distributed optical fiber temperature sensor with supervisory control and data acquisition （SCADA） system is proposed and implemented. In the implementation of the integration, both the compatibility with traditional system and the characteristics of distributed optical fiber temperature sensor is considered before Modbus TCP/IP protocol is chosen. The protocol is implemented with open source component Indy. The Modbus TCP/IP protocol used in the system is proved to be fast and robust.
文摘Background： Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells （EPCs） derived from human early fetal aortas in rat injured arteries. Methods： The carotid arterial endothelium of Sprague-Dawley rats was damaged by dilatation with a 1.5 F balloon catheter, and then EPCs derived from human early fetal aortas （〈14 weeks） were injected into the lumen of the injured artery in transplanted rats, with an equal volume of normal saline injected into control rats. Rats were sacrificed at 2 and 4 weeks after treatment and transplanted cells were identified by immunohistochemical staining with anti-human CD31 and anti-human mitochondria antibodies. Arterial cross-sections were analyzed by pathology, immunohistochemistry, and morphometry. Results： Green fluorescence-labeled EPCs could be seen in the endovascular surface of balloon-injured vessels after transplantation. The intimal area and intimal/medial area ratio were significantly smaller in the transplanted group than in the control （P 〈 0.05） and the residual lumen area was larger （P 〈 0.05）. After EPC transplantation, a complete vascular endothelial layer was formed, which was positive for human yon Willebrand factor after immunohistochemical staining, and immunohistochemical staining revealed many CD31- and mitochondria-positive cells in the re-endothelialized endothelium with EPC transplantation but not control treatment. Conclusion： EPCs derived from human early fetal aorta were successfully transplanted into injured vessels and might inhibit neointimal hyperplasia after vascular injury.
