A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture bro...A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.展开更多
Introduction Streptococcus agalactiae is most commonly associated with pregnancy-related infections,neonatal sepsis,and infections in patients with immunocompromised states,such as diabetes mellitus and malignancies.1...Introduction Streptococcus agalactiae is most commonly associated with pregnancy-related infections,neonatal sepsis,and infections in patients with immunocompromised states,such as diabetes mellitus and malignancies.1 Although abscesses caused by S.agalactiae are sometimes encountered in immunocompromised patients,S.agalactiae abscess in transplant recipients is extremely rare.S.agalactiae abscesses have never been reported in liver transplant recipients.In this article,we report a case of S.agalactiae abscess in the anterior cervical space extending to the superior mediastinum in a liver transplant recipient.展开更多
基金supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China,the Research Grant Council Grant,the University Development Fund,the Outstanding Young Researcher Award,and the Committee for Research and Conference Grant and The University of Hong Kong,China
文摘A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.
基金This work is partly supported by funding from the Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,the Ministry of Education of China.
文摘Introduction Streptococcus agalactiae is most commonly associated with pregnancy-related infections,neonatal sepsis,and infections in patients with immunocompromised states,such as diabetes mellitus and malignancies.1 Although abscesses caused by S.agalactiae are sometimes encountered in immunocompromised patients,S.agalactiae abscess in transplant recipients is extremely rare.S.agalactiae abscesses have never been reported in liver transplant recipients.In this article,we report a case of S.agalactiae abscess in the anterior cervical space extending to the superior mediastinum in a liver transplant recipient.
