Spindle cell carcinoma is a rare highly malignant squamous cell carcinoma.Here,we describe a case of a 74-yearold Chinese female who presented with a 2-week history of pain and swelling in the left retromolar region.S...Spindle cell carcinoma is a rare highly malignant squamous cell carcinoma.Here,we describe a case of a 74-yearold Chinese female who presented with a 2-week history of pain and swelling in the left retromolar region.Surgical resection and titanium plate prosthesis were performed and histological analysis revealed spindle squamous cell carcinoma.展开更多
Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncodin...Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.展开更多
Most of the patients with oral and maxillofacial malignancy are in the middle and advanced stages at diagnosis and the incidence rate is increasing in recent years.Chemotherapy alone is difficult to benefit the surviv...Most of the patients with oral and maxillofacial malignancy are in the middle and advanced stages at diagnosis and the incidence rate is increasing in recent years.Chemotherapy alone is difficult to benefit the survival of patients with advanced oral and maxillofacial malignancy.Ultrasound hyperthermia is a new and effective treatment for malignant tumor,which is developing rapidly in addition to conventional treatment.However,at present,ultrasound hyperthermia has not been widely used in the treatment of oral and maxillofacial malignancy.Therefore,formation of a guideline on ultrasound hyperthermia for oral and maxillofacial malignancy is mandatory,in order to promote and standardize the clinical practice of ultrasound hyperthermia in this field,and improve the long-term survival rate and quality of life of patients.展开更多
文摘Spindle cell carcinoma is a rare highly malignant squamous cell carcinoma.Here,we describe a case of a 74-yearold Chinese female who presented with a 2-week history of pain and swelling in the left retromolar region.Surgical resection and titanium plate prosthesis were performed and histological analysis revealed spindle squamous cell carcinoma.
基金supported by Jiangsu Commission of Health(M2020046).
文摘Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.
文摘Most of the patients with oral and maxillofacial malignancy are in the middle and advanced stages at diagnosis and the incidence rate is increasing in recent years.Chemotherapy alone is difficult to benefit the survival of patients with advanced oral and maxillofacial malignancy.Ultrasound hyperthermia is a new and effective treatment for malignant tumor,which is developing rapidly in addition to conventional treatment.However,at present,ultrasound hyperthermia has not been widely used in the treatment of oral and maxillofacial malignancy.Therefore,formation of a guideline on ultrasound hyperthermia for oral and maxillofacial malignancy is mandatory,in order to promote and standardize the clinical practice of ultrasound hyperthermia in this field,and improve the long-term survival rate and quality of life of patients.
