It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated w...It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated with single-chain Fv antibody against γ-seminoprotein.The resultant nanoprobes showed highly selective targeting,fluorescent imaging,and magnetic resonance imaging.The cytotoxicity effects were investigated on the prostate cancer cells and solid tumors under in vitro alternating magnetic field irradiation.It was found that the as-prepared nanoprobes did not show signs of toxicity within the used maximal dosage.It was also observed that the tumors implanted in nude mice were significantly reduced in size and disappeared gradually due to thermal treatment.The lifespan of post-therapeutic mice loaded with prostate cancer was considerable prolonged.High-performance single-chain Fv antibody againstγ-seminoprotein-conjugated fluorescent magnetic nanoparticles may have great potential in applications such early detection and localized thermal therapy of prostate cancer.展开更多
With the aim of investigating the ability and possible mechanism of Chinese medicine Jinkuishenqiwan pills to inhibit the spermatgoenic arrest infertile,100 mature male rats were randomly divided into five groups:norm...With the aim of investigating the ability and possible mechanism of Chinese medicine Jinkuishenqiwan pills to inhibit the spermatgoenic arrest infertile,100 mature male rats were randomly divided into five groups:normal control group,model group,test group treated with high dosage of Jinkuishenqiwan pills,test group treated with middle dosage of Jinkuishenqiwan pills,and test group treated with low dosage of Jinkuishenqiwan pills.Except the normal group,the other four groups were made into model of kidney-yang deficiency by injecting hydrocortisone.Simultaneously,the treatment of Jinkuishenqiwan pills was also irrigated into the stomachs of the three treated groups.After 30 days,the concentration of testosterone,the sperm density and the motility rate of sperm were examined,the microstructure of testicle was observed by optical microscope.Results showed that Jinkuishenqiwan pills can inhibit the spermatgoenic arrest infertile,there exists statistical difference between test group and the model group(P<0.05).In conclusion:Chinese medicine Jinkuishenqiwan pills may have a certain therapeutic effects on the rats with kidney-yang deficiency.展开更多
基金supported by China National 973 Project(2010CB933901)National 863 Hi-tech Project(2007AA022004)+1 种基金National Natural Scientific Fund(Nos.20771075and 30672147)the science and technology commission Fund of shanghai government(No.072112006 and No.0752nm024).
文摘It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated with single-chain Fv antibody against γ-seminoprotein.The resultant nanoprobes showed highly selective targeting,fluorescent imaging,and magnetic resonance imaging.The cytotoxicity effects were investigated on the prostate cancer cells and solid tumors under in vitro alternating magnetic field irradiation.It was found that the as-prepared nanoprobes did not show signs of toxicity within the used maximal dosage.It was also observed that the tumors implanted in nude mice were significantly reduced in size and disappeared gradually due to thermal treatment.The lifespan of post-therapeutic mice loaded with prostate cancer was considerable prolonged.High-performance single-chain Fv antibody againstγ-seminoprotein-conjugated fluorescent magnetic nanoparticles may have great potential in applications such early detection and localized thermal therapy of prostate cancer.
基金supported by grants from the National Scientific Foundation of China(39900126,30772683)the Natural Science Foundation of Shaanxi Province(SJ08-ZT10)the Tackle Key Problem in Scientific and Technological Program of Shaanxi Province(2008K04-02).
文摘With the aim of investigating the ability and possible mechanism of Chinese medicine Jinkuishenqiwan pills to inhibit the spermatgoenic arrest infertile,100 mature male rats were randomly divided into five groups:normal control group,model group,test group treated with high dosage of Jinkuishenqiwan pills,test group treated with middle dosage of Jinkuishenqiwan pills,and test group treated with low dosage of Jinkuishenqiwan pills.Except the normal group,the other four groups were made into model of kidney-yang deficiency by injecting hydrocortisone.Simultaneously,the treatment of Jinkuishenqiwan pills was also irrigated into the stomachs of the three treated groups.After 30 days,the concentration of testosterone,the sperm density and the motility rate of sperm were examined,the microstructure of testicle was observed by optical microscope.Results showed that Jinkuishenqiwan pills can inhibit the spermatgoenic arrest infertile,there exists statistical difference between test group and the model group(P<0.05).In conclusion:Chinese medicine Jinkuishenqiwan pills may have a certain therapeutic effects on the rats with kidney-yang deficiency.
