This study aimed to examine the effects of bioprocessing on the bioactive components of isoflavone-enriched soybean leaves(IESLs)using Tricholoma matsutake mycelia.To optimize fermentation conditions,various brown ric...This study aimed to examine the effects of bioprocessing on the bioactive components of isoflavone-enriched soybean leaves(IESLs)using Tricholoma matsutake mycelia.To optimize fermentation conditions,various brown rice ratios and fermentation periods were compared.Subsequently,a ratio of 8:2(aglycones:1873.57μg/g)soybean leaves to brown rice and a fermentation period of 8 days(aglycones:3623.58μg/g)were determined to be optimal for efficient fermentation conditions.Isoflavone derivatives were altered throughout the bio-processing process,with the fermentation stage indicating a bioconversion to isoflavone-aglycones.Using time-of-flight mass spectrometry,twenty functional metabolites were identified.The heat maps indicated a significant concentration of glycoside phytoestrogens at the fermentation stage during bioprocessing.The highest levels of total phenolics(8.49 GAE mg/g),total flavonoids(2.87 RE mg/g),and digestive enzymatic inhibitory activities(1 mg/mL;α-glucosidase 73.25%,pancreatic-lipase 58.26%)were seen in fermented IESLs.This study discovered that bioprocessing IESLs with T.matsutake mycelia has the potential to enhance aglycone phytochemicals in the bioproducts.展开更多
基金supported by the Basic Science Research Program through the National Research Foundation(NRF)funded by the Ministry of Education(Grant number 2016R1D1A1B01009898 and RS-2023-00245096),Republic of Korea.
文摘This study aimed to examine the effects of bioprocessing on the bioactive components of isoflavone-enriched soybean leaves(IESLs)using Tricholoma matsutake mycelia.To optimize fermentation conditions,various brown rice ratios and fermentation periods were compared.Subsequently,a ratio of 8:2(aglycones:1873.57μg/g)soybean leaves to brown rice and a fermentation period of 8 days(aglycones:3623.58μg/g)were determined to be optimal for efficient fermentation conditions.Isoflavone derivatives were altered throughout the bio-processing process,with the fermentation stage indicating a bioconversion to isoflavone-aglycones.Using time-of-flight mass spectrometry,twenty functional metabolites were identified.The heat maps indicated a significant concentration of glycoside phytoestrogens at the fermentation stage during bioprocessing.The highest levels of total phenolics(8.49 GAE mg/g),total flavonoids(2.87 RE mg/g),and digestive enzymatic inhibitory activities(1 mg/mL;α-glucosidase 73.25%,pancreatic-lipase 58.26%)were seen in fermented IESLs.This study discovered that bioprocessing IESLs with T.matsutake mycelia has the potential to enhance aglycone phytochemicals in the bioproducts.
