Tibet is not only the origin of rivers,the source of ecology in South and Southeast Asia,but also an initiator and a conditioning zone for the Chinese and even the climate of the Eastern Hemisphere,thus honored as the...Tibet is not only the origin of rivers,the source of ecology in South and Southeast Asia,but also an initiator and a conditioning zone for the Chinese and even the climate of the Eastern Hemisphere,thus honored as the last pure land in the world.Tibet is known as grand,magnificent,pure and mysterious,展开更多
The Lhasa-Shigatse Railway took 4 years to build.In those 1,400 days,while solving the technical problems,the builders tried every means to help the Tibetan locals.As the saying goes,he who sows courtesy reaps friends...The Lhasa-Shigatse Railway took 4 years to build.In those 1,400 days,while solving the technical problems,the builders tried every means to help the Tibetan locals.As the saying goes,he who sows courtesy reaps friendship.Their genuine efforts won them real friends among the Tibetan locals.Lhabupu Township of展开更多
Salinity is one of the most severe environmental factors that may impair crop productivity.A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress ...Salinity is one of the most severe environmental factors that may impair crop productivity.A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila,an Arabidopsis-related halophyte.Four-week-old seedlings are exposed to long-term salinity treatment.The total crude proteins are extracted from leaf blades,separated by 2-DE,stained with Coomassie Brilliant Blue,and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS.Among 900 protein spots reproducibly detected on each gel,30 spots exhibit significant change and some of them are identified.The identified proteins include not only some previously characterized stress-responsive proteins such as TIR-NBS-LRR class disease resistance protein,ferritin-1,and pathogenesis-related protein 5,but also some proteins related to energy pathway,metabolism,RNA processing and protein degradation,as well as proteins with unknown functions.The possible functions of these proteins in salinity tolerance of T.halophila are discussed and it is suggested that the long-term salinity tolerance of T.halophila is achieved,at least partly,by enhancing defense system,adjusting energy and metabolic pathway and maintaining RNA structure.展开更多
Kinetochores are large proteinaceous structure on the surface of chromosomes’primary constriction during mitosis.They link chromosomes to spindle microtubules and also regulate the spindle assem-bly checkpoint,which ...Kinetochores are large proteinaceous structure on the surface of chromosomes’primary constriction during mitosis.They link chromosomes to spindle microtubules and also regulate the spindle assem-bly checkpoint,which is crucial for correct chromosome segregation in all eukaryotes.The better known core networks of kinetochores include the KMN network(K,KNL1;M,Mis12 complex;N,Ndc80 complex)and CCAN(constitutive centromere-associated network).However,the detailed molecular mechanism of the kinetochore protein network remains unclear.This study demonstrates that CENP-H and CENP-K form quite stable subcomplex by TAP(tandem affinity purification)with HEK 293 cells which express TAP-CENP-K,with the ratio of purified CENP-H and CENP-K being close to 1︰1 even with high salt.Bioinformatic analysis suggests that CENP-H and CENP-K are enriched with coiled-coil regions.This implies that CENP-H and CENP-K form heterodimeric coiled-coils.Furthermore,the func-tional regions which form the complex are respectively located on their N-and C-terminals,but the association between the C-terminals is more complex.It is possible that this is the first identified het-erodimeric coiled-coils within the inner kinetochore,which is directly involved in the attachment be-tween kinetochores and the spindle microtubules.展开更多
Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome-try(SELDI-TOF-MS).The da...Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome-try(SELDI-TOF-MS).The data analyzed by both Biomarker Wizard?and Biomarker Patterns?software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased in lung cancer.Meanwhile,the level of this biomarker was progressively increased with the clinical stages of lung cancer.The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sul-fate-polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein(SAA)by MALDI/MS-MS and database searching.It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody.To confirm the SAA differential expression in lung cancer patients,the same set of serum samples was measured by ELISA assay.The result showed that at the cutoff point 0.446(OD value)on the Receiver Operating Characteristic(ROC)curve,SAA could better discriminate lung cancer from healthy indi-viduals with sensitivity of 84.1%and specificity of 80%.These findings demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer.展开更多
Compared to other subcellular organelles,centrosome proteome can hardly be studied,due to the dif-ficulties in separation and purification of centrosome.Auto-antisera from 6 autoimmune patients,which recognized centro...Compared to other subcellular organelles,centrosome proteome can hardly be studied,due to the dif-ficulties in separation and purification of centrosome.Auto-antisera from 6 autoimmune patients,which recognized centrosome specifically in immunofluorescence,were used to identify the corresponding centrosomal proteins.The sera were first tested by Western blot on whole cell lysate,and all bound antibodies were then eluted from each single band in Western blot membrane to assure which antibody was responsible for the centrosome specific immunofluorescence staining.The corresponding pro-teins were obtained by immunoprecipitation and identified by mass spectrometry.Six centrosomal proteins,including 2 known centrosomal proteins and 4 proteins with unknown localization or report-edly non-centrosomal localization,were identified.These proteins apparently involve in cell cycle regulation,signal transduction pathways,molecular chaperons,and metabolism enzymes,which may reflect the expected functional diversity of centrosome.展开更多
There are multiple reports of autoimmune response in patients with lung cancer.To investigate whether a novel autoantibody is present in patients with lung cancer and evaluate its clinical diagnostic and prognostic va...There are multiple reports of autoimmune response in patients with lung cancer.To investigate whether a novel autoantibody is present in patients with lung cancer and evaluate its clinical diagnostic and prognostic value,sera from 10 patients with lung cancer and 10 normal individuals were analyzed using immunofluorescence and Western blotting.It was found that one serum sample from the patients with squamous carcinoma gave a fine speckled pattern staining in nucleus and had a high titer antinuclear autoantibody which could recognize 31 kD of nuclear protein isolated from both cancer cells and normal cells.The same patient’s serum was further used to immunoprecipitate the target antigen.The protein bands were excised from the SDS-PAGE gels and were analyzed with a Qstar Pulser I Quadrupole time-flight mass spec-trometer,and the 31 kD target antigen was identified as U1-A snRNP.To test the prevalence of anti-U1-A snRNP antibody,sera from 93 patients including 36 squmaous carcinomas(SCC),26 adenocarcinomas(Ad),and 31 small cell carcinomas(SCLC)were screened by Western blotting.The results demonstrated that anti-U1-A snRNP antibody was present in 50%of SCC sera,26.9%of Ad sera and 54.8%of SCLC sera.In this paper,we report for the first time that anti-U1-A snRNP antibody could be detected in the patients with lung cancer.展开更多
文摘Tibet is not only the origin of rivers,the source of ecology in South and Southeast Asia,but also an initiator and a conditioning zone for the Chinese and even the climate of the Eastern Hemisphere,thus honored as the last pure land in the world.Tibet is known as grand,magnificent,pure and mysterious,
文摘The Lhasa-Shigatse Railway took 4 years to build.In those 1,400 days,while solving the technical problems,the builders tried every means to help the Tibetan locals.As the saying goes,he who sows courtesy reaps friendship.Their genuine efforts won them real friends among the Tibetan locals.Lhabupu Township of
基金the National Basic Research and Development Program of China(Grant Nos.2006CB100100,2004CB117303)National Natural Science Foundation of China(Grants Nos.30670203,30570434)+1 种基金Beijing Municipal National Natural Science Foundation(Grant No.5062021)CUN 985-3-3 and Open Fund of Key Laboratory of Cell Proliferation and Regulation Biology,Ministry of Education of China.
文摘Salinity is one of the most severe environmental factors that may impair crop productivity.A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila,an Arabidopsis-related halophyte.Four-week-old seedlings are exposed to long-term salinity treatment.The total crude proteins are extracted from leaf blades,separated by 2-DE,stained with Coomassie Brilliant Blue,and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS.Among 900 protein spots reproducibly detected on each gel,30 spots exhibit significant change and some of them are identified.The identified proteins include not only some previously characterized stress-responsive proteins such as TIR-NBS-LRR class disease resistance protein,ferritin-1,and pathogenesis-related protein 5,but also some proteins related to energy pathway,metabolism,RNA processing and protein degradation,as well as proteins with unknown functions.The possible functions of these proteins in salinity tolerance of T.halophila are discussed and it is suggested that the long-term salinity tolerance of T.halophila is achieved,at least partly,by enhancing defense system,adjusting energy and metabolic pathway and maintaining RNA structure.
基金Supported by the National Key Scientific Program(Grant No.2006CB910100).
文摘Kinetochores are large proteinaceous structure on the surface of chromosomes’primary constriction during mitosis.They link chromosomes to spindle microtubules and also regulate the spindle assem-bly checkpoint,which is crucial for correct chromosome segregation in all eukaryotes.The better known core networks of kinetochores include the KMN network(K,KNL1;M,Mis12 complex;N,Ndc80 complex)and CCAN(constitutive centromere-associated network).However,the detailed molecular mechanism of the kinetochore protein network remains unclear.This study demonstrates that CENP-H and CENP-K form quite stable subcomplex by TAP(tandem affinity purification)with HEK 293 cells which express TAP-CENP-K,with the ratio of purified CENP-H and CENP-K being close to 1︰1 even with high salt.Bioinformatic analysis suggests that CENP-H and CENP-K are enriched with coiled-coil regions.This implies that CENP-H and CENP-K form heterodimeric coiled-coils.Furthermore,the func-tional regions which form the complex are respectively located on their N-and C-terminals,but the association between the C-terminals is more complex.It is possible that this is the first identified het-erodimeric coiled-coils within the inner kinetochore,which is directly involved in the attachment be-tween kinetochores and the spindle microtubules.
基金the Major State Basic Research Program of China(Grant No.2006CB910100)National Natural Science Foundation of China(Grant No.30370712)+1 种基金Natural Science Foundation of Beijing(Grant No.7051002)Sci-ence Technology Committee of Beijing(Grant No.Y0204002040111)
文摘Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome-try(SELDI-TOF-MS).The data analyzed by both Biomarker Wizard?and Biomarker Patterns?software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased in lung cancer.Meanwhile,the level of this biomarker was progressively increased with the clinical stages of lung cancer.The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sul-fate-polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein(SAA)by MALDI/MS-MS and database searching.It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody.To confirm the SAA differential expression in lung cancer patients,the same set of serum samples was measured by ELISA assay.The result showed that at the cutoff point 0.446(OD value)on the Receiver Operating Characteristic(ROC)curve,SAA could better discriminate lung cancer from healthy indi-viduals with sensitivity of 84.1%and specificity of 80%.These findings demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer.
基金the special fundations of Ministry of Education of China and National Natural Science Foundation of China(Grant No.30225017)
文摘Compared to other subcellular organelles,centrosome proteome can hardly be studied,due to the dif-ficulties in separation and purification of centrosome.Auto-antisera from 6 autoimmune patients,which recognized centrosome specifically in immunofluorescence,were used to identify the corresponding centrosomal proteins.The sera were first tested by Western blot on whole cell lysate,and all bound antibodies were then eluted from each single band in Western blot membrane to assure which antibody was responsible for the centrosome specific immunofluorescence staining.The corresponding pro-teins were obtained by immunoprecipitation and identified by mass spectrometry.Six centrosomal proteins,including 2 known centrosomal proteins and 4 proteins with unknown localization or report-edly non-centrosomal localization,were identified.These proteins apparently involve in cell cycle regulation,signal transduction pathways,molecular chaperons,and metabolism enzymes,which may reflect the expected functional diversity of centrosome.
基金This work was supported by the National Natural Science Foundation of China(Grant No.30370712)863 Project(Grant No.2002AA232031).
文摘There are multiple reports of autoimmune response in patients with lung cancer.To investigate whether a novel autoantibody is present in patients with lung cancer and evaluate its clinical diagnostic and prognostic value,sera from 10 patients with lung cancer and 10 normal individuals were analyzed using immunofluorescence and Western blotting.It was found that one serum sample from the patients with squamous carcinoma gave a fine speckled pattern staining in nucleus and had a high titer antinuclear autoantibody which could recognize 31 kD of nuclear protein isolated from both cancer cells and normal cells.The same patient’s serum was further used to immunoprecipitate the target antigen.The protein bands were excised from the SDS-PAGE gels and were analyzed with a Qstar Pulser I Quadrupole time-flight mass spec-trometer,and the 31 kD target antigen was identified as U1-A snRNP.To test the prevalence of anti-U1-A snRNP antibody,sera from 93 patients including 36 squmaous carcinomas(SCC),26 adenocarcinomas(Ad),and 31 small cell carcinomas(SCLC)were screened by Western blotting.The results demonstrated that anti-U1-A snRNP antibody was present in 50%of SCC sera,26.9%of Ad sera and 54.8%of SCLC sera.In this paper,we report for the first time that anti-U1-A snRNP antibody could be detected in the patients with lung cancer.
