Background The cellular basis of testicular development and spermatogenesis for the extreme sperm density in chickens(100-fold higher than mammals)remains poorly defined.A comprehensive understanding of the molecular ...Background The cellular basis of testicular development and spermatogenesis for the extreme sperm density in chickens(100-fold higher than mammals)remains poorly defined.A comprehensive understanding of the molecular characteristics driving poultry testicular development is crucial for explaining this enhanced spermatogenic capacity.Results Here,we first established a single-cell transcriptome profile of chicken testes from hatching to maturity,identifying the dynamic transcriptional characteristics of germ cell fate transition and exploring the developmental characteristics of Sertoli cells and Leydig cells.Multi-species comparisons revealed a higher proportion of germ cells and the unique adaptations of Sertoli cells in chicken testes.Most importantly,our results demonstrated that Sertoli cells dominated in somatic composition of mature chicken testes,and proliferating Sertoli cells persisted in chicken testes even after sexual maturity,while no proliferating Sertoli cells in mammals.We also found a richer interaction network between chicken testicular cells,especially the specific activation of Sertoli cell interaction signals,such as TGF-β,BMP,EGF,and activin.These adaptations of Sertoli cells may support the spermatogenic superiority in chickens.Additionally,our results indicated that cAMP responsive element binding protein 5(CREB5)played a crucial role in maintaining the maturation and function of chicken Sertoli cells,and circadian rhythm promoted testosterone secretion and the development of Leydig cells.Conclusion Our study revealed that the sustained proliferative capacity of Sertoli cells,their enriched signaling network,and the regulatory roles of CREB5 and circadian rhythms collectively represented unique testicular adaptations in chickens.These findings may hold extraordinary significance in understanding the molecular characteristics of poultry testicular development,and provide a plausible framework for explaining enhanced spermatogenesis in poultry.展开更多
A multiproxy approach including bulk organic carbon, δ^(13) C, lignin phenols, and lipid biomarker analyses were applied to characterize the source and composition of sedimentary organic matter in the head part of Th...A multiproxy approach including bulk organic carbon, δ^(13) C, lignin phenols, and lipid biomarker analyses were applied to characterize the source and composition of sedimentary organic matter in the head part of Three Gorges Reservoir. OM consisted of both natural(autochthonous and allochthonous) and anthropogenic inputs.The natural OM included input from vascular plants,especially non-woody angiosperms. The allochthonous input included plankton and microbial production, likely due to localized eutrophication. Anthropogenic inputs likely derived from petroleum input and/or urban activities.Other anthropogenic inputs were untreated sewage waste.These influences were concluded to be regionally specific point sources of pollution based on relative distributions and on the fact that molecular characteristics of sedimentary OM were not distributed smoothly along a gradient.展开更多
Since the discovery of enzyme-like activity of Fe3O4 nanoparticles in 2007,nanozymes are becoming the promising substitutes for natural enzymes due to their advantages of high catalytic activity,low cost,mild reaction...Since the discovery of enzyme-like activity of Fe3O4 nanoparticles in 2007,nanozymes are becoming the promising substitutes for natural enzymes due to their advantages of high catalytic activity,low cost,mild reaction conditions,good stability,and suitable for large-scale production.Recently,with the cross fusion of nanomedicine and nanocatalysis,nanozyme-based theranostic strategies attract great attention,since the enzymatic reactions can be triggered in the tumor microenvironment to achieve good curative effect with substrate specificity and low side effects.Thus,various nanozymes have been developed and used for tumor therapy.In this review,more than 270 research articles are discussed systematically to present progress in the past five years.First,the discovery and development of nanozymes are summarized.Second,classification and catalytic mechanism of nanozymes are discussed.Third,activity prediction and rational design of nanozymes are focused by highlighting the methods of density functional theory,machine learning,biomimetic and chemical design.Then,synergistic theranostic strategy of nanozymes are introduced.Finally,current challenges and future prospects of nanozymes used for tumor theranostic are outlined,including selectivity,biosafety,repeatability and stability,in-depth catalytic mechanism,predicting and evaluating activities.展开更多
植物修复是去除重金属污染最具前景的修复手段之一.为全面了解和掌握重金属污染植物修复的研究现状以及发展趋势,检索了2000年1月1日—2024年4月30日期间中国知网(CNKI)数据库和Web of Science(WOS)核心数据库中关于重金属植物修复的相...植物修复是去除重金属污染最具前景的修复手段之一.为全面了解和掌握重金属污染植物修复的研究现状以及发展趋势,检索了2000年1月1日—2024年4月30日期间中国知网(CNKI)数据库和Web of Science(WOS)核心数据库中关于重金属植物修复的相关文献,使用CiteSpace软件绘制重金属植物修复研究的知识图谱,从发文量、核心作者、研究机构、关键词和突现词等方面进行文献可视化分析.研究结果表明:自21世纪以来,重金属植物修复研究领域发文数量呈现增长态势,重金属植物修复正逐渐成为我国的研究热点;中国科学院土壤研究所的部分学者之间联系较为紧密,是重金属植物修复领域的核心学者;在发文机构统计方面,中南林业科技大学、南京农业大学、四川农业大学、湖南农业大学、中国科学院研究生院、西北农林科技大学、浙江大学等科研院所占据主要地位,并具有较强的影响力,但与国际研究机构相比,国内大学和研究所之间仍需强化学术交流和学术合作;重金属植物修复研究主要聚焦于土壤重金属污染,其中以生物修复、cadmium、phytoremediation等为主要研究内容;近五年重金属植物修复研究聚焦于利用“联合修复”“生态修复”和“植物内生菌”等手段来提高重金属污染植物修复的效率,进一步促进重金属植物修复技术的发展及实际应用.此研究有助于全面了解目前我国重金属植物修复技术的研究趋势,可为重金属植物修复的研究提供一定的参考和借鉴.展开更多
Lateral flow immunoassay(LFIA),a rapid detection technique noted for simplicity and economy,has showcased indispensable applicability in diverse domains such as disease screening,food safety,and environmental monitori...Lateral flow immunoassay(LFIA),a rapid detection technique noted for simplicity and economy,has showcased indispensable applicability in diverse domains such as disease screening,food safety,and environmental monitoring.Nevertheless,challenges still exist in detecting ultra-low concentration analytes due to the inherent sensitivity limitations of LFIA.Recently,significant advances have been achieved by integrating enzyme activity probes and transforming LFIA into a highly sensitive tool for rapidly detecting trace analyte concentrations.Specifically,modifying natural enzymes or engineered nanozymes allows them to function as immune probes,directly catalyzing the production of signal molecules or indirectly initiating enzyme activity.Therefore,the signal intensity and detection sensitivity of LFIA are markedly elevated.The present review undertakes a comprehensive examination of pertinent research literature,offering a systematic analysis of recently proposed enzyme-based signal amplification strategies.By way of comparative assessment,the merits and demerits of current approaches are delineated,along with the identification of research avenues that still need to be explored.It is anticipated that this critical overview will garner considerable attention within the biomedical and materials science communities,providing valuable direction and insight toward the advancement of high-performance LFIA technologies.展开更多
Implantable temperature sensors are revolutionizing physiological monitoring and playing a crucial role in diagnostics,therapeutics,and life sciences research.This review classifies the materials used in these sensors...Implantable temperature sensors are revolutionizing physiological monitoring and playing a crucial role in diagnostics,therapeutics,and life sciences research.This review classifies the materials used in these sensors into three categories:metal-based,inorganic semiconductor,and organic semiconductor materials.Metal-based materials are widely used in medical and industrial applications due to their linearity,stability,and reliability.Inorganic semiconductors provide rapid response times and high miniaturization potential,making them promising for biomedical and environmental monitoring.Organic semiconductors offer high sensitivity and ease of processing,enabling the development of flexible and stretchable sensors.This review analyzes recent studies for each material type,covering design principles,performance characteristics,and applications,highlighting key advantages and challenges regarding miniaturization,sensitivity,response time,and biocompatibility.Furthermore,critical performance parameters of implantable temperature sensors based on different material types are summarized,providing valuable references for future sensor design and optimization.The future development of implantable temperature sensors is discussed,focusing on improving biocompatibility,long-term stability,and multifunctional integration.These advancements are expected to expand the application potential of implantable sensors in telemedicine and dynamic physiological monitoring.展开更多
基金supported by“National Key Research and Development Program of China”(Grant No.2021YFF1000701)“National Natural Science Foundation of China”(Grant No.U22A20509)。
文摘Background The cellular basis of testicular development and spermatogenesis for the extreme sperm density in chickens(100-fold higher than mammals)remains poorly defined.A comprehensive understanding of the molecular characteristics driving poultry testicular development is crucial for explaining this enhanced spermatogenic capacity.Results Here,we first established a single-cell transcriptome profile of chicken testes from hatching to maturity,identifying the dynamic transcriptional characteristics of germ cell fate transition and exploring the developmental characteristics of Sertoli cells and Leydig cells.Multi-species comparisons revealed a higher proportion of germ cells and the unique adaptations of Sertoli cells in chicken testes.Most importantly,our results demonstrated that Sertoli cells dominated in somatic composition of mature chicken testes,and proliferating Sertoli cells persisted in chicken testes even after sexual maturity,while no proliferating Sertoli cells in mammals.We also found a richer interaction network between chicken testicular cells,especially the specific activation of Sertoli cell interaction signals,such as TGF-β,BMP,EGF,and activin.These adaptations of Sertoli cells may support the spermatogenic superiority in chickens.Additionally,our results indicated that cAMP responsive element binding protein 5(CREB5)played a crucial role in maintaining the maturation and function of chicken Sertoli cells,and circadian rhythm promoted testosterone secretion and the development of Leydig cells.Conclusion Our study revealed that the sustained proliferative capacity of Sertoli cells,their enriched signaling network,and the regulatory roles of CREB5 and circadian rhythms collectively represented unique testicular adaptations in chickens.These findings may hold extraordinary significance in understanding the molecular characteristics of poultry testicular development,and provide a plausible framework for explaining enhanced spermatogenesis in poultry.
基金supported by the"100"talent program of Zhejiang University to D.He under the Grant No.188020*194231701/008 and188020-193810201/102NSFC to Y.Sun under the Grant No.41372131
文摘A multiproxy approach including bulk organic carbon, δ^(13) C, lignin phenols, and lipid biomarker analyses were applied to characterize the source and composition of sedimentary organic matter in the head part of Three Gorges Reservoir. OM consisted of both natural(autochthonous and allochthonous) and anthropogenic inputs.The natural OM included input from vascular plants,especially non-woody angiosperms. The allochthonous input included plankton and microbial production, likely due to localized eutrophication. Anthropogenic inputs likely derived from petroleum input and/or urban activities.Other anthropogenic inputs were untreated sewage waste.These influences were concluded to be regionally specific point sources of pollution based on relative distributions and on the fact that molecular characteristics of sedimentary OM were not distributed smoothly along a gradient.
基金S.G.acknowledges the financial support from the National Natural Science Foundation of China(NSFC 52272144,51972076)the Heilongjiang Provincial Natural Science Foundation of China(JQ2022E001)+4 种基金the Natural Science Foundation of Shandong Province(ZR2020ZD42)the Fundamental Research Funds for the Central Universities.H.D.acknowledges the financial support from the National Natural Science Foundation of China(NSFC 22205048)China Postdoctoral Science Foundation(2022M710931 and 2023T160154)Heilongjiang Postdoctoral Science Foundation(LBH-Z22010)G.Y.acknowledges the financial support from the National Science Foundation of Heilongjiang Education Department(324022075).
文摘Since the discovery of enzyme-like activity of Fe3O4 nanoparticles in 2007,nanozymes are becoming the promising substitutes for natural enzymes due to their advantages of high catalytic activity,low cost,mild reaction conditions,good stability,and suitable for large-scale production.Recently,with the cross fusion of nanomedicine and nanocatalysis,nanozyme-based theranostic strategies attract great attention,since the enzymatic reactions can be triggered in the tumor microenvironment to achieve good curative effect with substrate specificity and low side effects.Thus,various nanozymes have been developed and used for tumor therapy.In this review,more than 270 research articles are discussed systematically to present progress in the past five years.First,the discovery and development of nanozymes are summarized.Second,classification and catalytic mechanism of nanozymes are discussed.Third,activity prediction and rational design of nanozymes are focused by highlighting the methods of density functional theory,machine learning,biomimetic and chemical design.Then,synergistic theranostic strategy of nanozymes are introduced.Finally,current challenges and future prospects of nanozymes used for tumor theranostic are outlined,including selectivity,biosafety,repeatability and stability,in-depth catalytic mechanism,predicting and evaluating activities.
文摘植物修复是去除重金属污染最具前景的修复手段之一.为全面了解和掌握重金属污染植物修复的研究现状以及发展趋势,检索了2000年1月1日—2024年4月30日期间中国知网(CNKI)数据库和Web of Science(WOS)核心数据库中关于重金属植物修复的相关文献,使用CiteSpace软件绘制重金属植物修复研究的知识图谱,从发文量、核心作者、研究机构、关键词和突现词等方面进行文献可视化分析.研究结果表明:自21世纪以来,重金属植物修复研究领域发文数量呈现增长态势,重金属植物修复正逐渐成为我国的研究热点;中国科学院土壤研究所的部分学者之间联系较为紧密,是重金属植物修复领域的核心学者;在发文机构统计方面,中南林业科技大学、南京农业大学、四川农业大学、湖南农业大学、中国科学院研究生院、西北农林科技大学、浙江大学等科研院所占据主要地位,并具有较强的影响力,但与国际研究机构相比,国内大学和研究所之间仍需强化学术交流和学术合作;重金属植物修复研究主要聚焦于土壤重金属污染,其中以生物修复、cadmium、phytoremediation等为主要研究内容;近五年重金属植物修复研究聚焦于利用“联合修复”“生态修复”和“植物内生菌”等手段来提高重金属污染植物修复的效率,进一步促进重金属植物修复技术的发展及实际应用.此研究有助于全面了解目前我国重金属植物修复技术的研究趋势,可为重金属植物修复的研究提供一定的参考和借鉴.
基金Financial supports from the National Natural Science Foundation of China(NSFC,Nos.52272144 and 22205048)Heilongjiang Provincial Natural Science Foundation of China(No.JQ2022E001)+3 种基金China Postdoctoral Science Foundation(Nos.2022M710931 and 2023T160154)Heilongjiang Postdoctoral Science Foundation(No.LBH-Z22010)Natural Science Foundation of Shandong Province(No.ZR2020ZD42)the Fundamental Research funds for the Central Universities are greatly acknowledged.
文摘Lateral flow immunoassay(LFIA),a rapid detection technique noted for simplicity and economy,has showcased indispensable applicability in diverse domains such as disease screening,food safety,and environmental monitoring.Nevertheless,challenges still exist in detecting ultra-low concentration analytes due to the inherent sensitivity limitations of LFIA.Recently,significant advances have been achieved by integrating enzyme activity probes and transforming LFIA into a highly sensitive tool for rapidly detecting trace analyte concentrations.Specifically,modifying natural enzymes or engineered nanozymes allows them to function as immune probes,directly catalyzing the production of signal molecules or indirectly initiating enzyme activity.Therefore,the signal intensity and detection sensitivity of LFIA are markedly elevated.The present review undertakes a comprehensive examination of pertinent research literature,offering a systematic analysis of recently proposed enzyme-based signal amplification strategies.By way of comparative assessment,the merits and demerits of current approaches are delineated,along with the identification of research avenues that still need to be explored.It is anticipated that this critical overview will garner considerable attention within the biomedical and materials science communities,providing valuable direction and insight toward the advancement of high-performance LFIA technologies.
基金supported by the National Natural Science Foundation of China(NSFC)(62422501)Beijing Nova Program(20230484254,20240484742)Hebei Natural Science Foundation(F2024105039).
文摘Implantable temperature sensors are revolutionizing physiological monitoring and playing a crucial role in diagnostics,therapeutics,and life sciences research.This review classifies the materials used in these sensors into three categories:metal-based,inorganic semiconductor,and organic semiconductor materials.Metal-based materials are widely used in medical and industrial applications due to their linearity,stability,and reliability.Inorganic semiconductors provide rapid response times and high miniaturization potential,making them promising for biomedical and environmental monitoring.Organic semiconductors offer high sensitivity and ease of processing,enabling the development of flexible and stretchable sensors.This review analyzes recent studies for each material type,covering design principles,performance characteristics,and applications,highlighting key advantages and challenges regarding miniaturization,sensitivity,response time,and biocompatibility.Furthermore,critical performance parameters of implantable temperature sensors based on different material types are summarized,providing valuable references for future sensor design and optimization.The future development of implantable temperature sensors is discussed,focusing on improving biocompatibility,long-term stability,and multifunctional integration.These advancements are expected to expand the application potential of implantable sensors in telemedicine and dynamic physiological monitoring.
