The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of...The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.展开更多
In recent years,silver nanoparticles(Ag NPs)had produced by biological methods such as plant extract due to their efficiency,low cost,being non-toxic,and ecofriendly nature.Ag NPs have antibacterial,anti-mold,and anti...In recent years,silver nanoparticles(Ag NPs)had produced by biological methods such as plant extract due to their efficiency,low cost,being non-toxic,and ecofriendly nature.Ag NPs have antibacterial,anti-mold,and anti-fungi because of their high surface area to volume ratio.In this study,Opuntia ficus-indica(Prickly pear)extract was used to produce green synthesized Ag NPs.Different techniques had adopted to describe the generated nanoparticles,such as an ultraviolet spectrophotometer,Transmission Electron Microscopy(TEM),Dynamic Light Scattering(DLS),and Zeta Potential Analysis.Escherichia coli(E.coli),separated from sediment and water of the Hillah River in Babylon city in Iraq,was utilizedto estimate the antibacterial activities of Ag NPs at different concentrations.Both broth microdilution assay and well diffusion assay were applied.The Congo Red Agar implied to investigate the ability of E.coli isolates to form a biofilm.The TEM images of Ag NPs illustrated spherical morphology with a diameter of approximately 22±4 nm.The antibacterial activity tests showed that the Minimum Inhibitory Concentration(MIC)ranged from 0.0125 mg·L^(-1) to 0.05 mg·L^(-1),whereas Minimum Bactericidal Concentration(MBC)was from 0.025 mg·L^(-1) to 0.05 mg·L^(-1).5 h ofexposure to the 0.025 mg·L^(-1) concentrations of the Ag NPs had a bactericidal impact on 92%of the E.coli isolates.In our study,we found silver nanoparticles synthesized by Opuntia ficus-Indica have antibacterial activity against waterborne Escherichia coli isolates and it could be utilizedtoreduce microbial growth in contaminated water.展开更多
文摘The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.
文摘In recent years,silver nanoparticles(Ag NPs)had produced by biological methods such as plant extract due to their efficiency,low cost,being non-toxic,and ecofriendly nature.Ag NPs have antibacterial,anti-mold,and anti-fungi because of their high surface area to volume ratio.In this study,Opuntia ficus-indica(Prickly pear)extract was used to produce green synthesized Ag NPs.Different techniques had adopted to describe the generated nanoparticles,such as an ultraviolet spectrophotometer,Transmission Electron Microscopy(TEM),Dynamic Light Scattering(DLS),and Zeta Potential Analysis.Escherichia coli(E.coli),separated from sediment and water of the Hillah River in Babylon city in Iraq,was utilizedto estimate the antibacterial activities of Ag NPs at different concentrations.Both broth microdilution assay and well diffusion assay were applied.The Congo Red Agar implied to investigate the ability of E.coli isolates to form a biofilm.The TEM images of Ag NPs illustrated spherical morphology with a diameter of approximately 22±4 nm.The antibacterial activity tests showed that the Minimum Inhibitory Concentration(MIC)ranged from 0.0125 mg·L^(-1) to 0.05 mg·L^(-1),whereas Minimum Bactericidal Concentration(MBC)was from 0.025 mg·L^(-1) to 0.05 mg·L^(-1).5 h ofexposure to the 0.025 mg·L^(-1) concentrations of the Ag NPs had a bactericidal impact on 92%of the E.coli isolates.In our study,we found silver nanoparticles synthesized by Opuntia ficus-Indica have antibacterial activity against waterborne Escherichia coli isolates and it could be utilizedtoreduce microbial growth in contaminated water.
