Hyperpolarization of nuclear spins is crucial for advancing nuclear magnetic resonance and quantum information technologies,as nuclear spins typically exhibit extremely low polarization at room temperature due to thei...Hyperpolarization of nuclear spins is crucial for advancing nuclear magnetic resonance and quantum information technologies,as nuclear spins typically exhibit extremely low polarization at room temperature due to their small gyromagnetic ratios.A promising approach to achieving high nuclear spin polarization is transferring the polarization of electrons to nuclear spins.The nitrogen-vacancy(NV)center in diamond has emerged as a highly effective medium for this purpose,and various hyperpolarization protocols have been developed.Among these,the pulsed polarization(PulsePol)method has been extensively studied due to its robustness against static energy shifts of the electron spin.In this work,we present a novel polarization protocol and uncover a family of magic sequences for hyperpolarizing nuclear spins,with PulsePol emerging as a special case of our general approach.Notably,we demonstrate that some of these magic sequences exhibit significantly greater robustness compared to the PulsePol protocol in the presence of finite half𝜋pulse duration of the protocol,Rabi and detuning errors.This enhanced robustness positions our protocol as a more suitable candidate for hyper-polarizing nuclear spins species with large gyromagnetic ratios and also ensures better compatibility with high-efficiency readout techniques at high magnetic fields.Additionally,the generality of our protocol allows for its direct application to other solid-state quantum systems beyond the NV center.展开更多
BACKGROUND Whether rtS106C+H126Y+D134E/rtS106C+H126Y+D134E+L269I(rtCYE/rtCYEI)mutations in the hepatitis B virus(HBV)reverse-transcriptase(RT)region are associated with tenofovir disoproxil fumarate(TDF)resistance is ...BACKGROUND Whether rtS106C+H126Y+D134E/rtS106C+H126Y+D134E+L269I(rtCYE/rtCYEI)mutations in the hepatitis B virus(HBV)reverse-transcriptase(RT)region are associated with tenofovir disoproxil fumarate(TDF)resistance is controversial.AIM To evaluate the presence of the rtCYE/rtCYEI mutations in a large cohort of Chinese patients with chronic HBV infection.METHODS A total of 28236 patients who underwent drug resistance testing at the Fifth Medical Center of Chinese PLA General Hospital from 2007 to 2019 were enrolled.All patients received nucleoside/nucleotide analogues(NAs)therapy,and serum samples were collected for sequence analysis of the HBV RT domain with mutation analysis.RESULTS The detection rates of a single mutation of rtS106C,rtH126Y,rtD134E,and rtL269I were 8.21%,3.20%,2.55%and 61.49%in 23718 genotype C patients,and 1.31%,1.76%,0.21%,and 92.33%in 4266 genotype B patients,respectively.The combined mutations of rtCYE/rtCYEI were only detected in 12 genotype C patients,accounting for 0.042%of all patients.These 12 patients had received NA treatments except TDF before testing.Among them,6 patients had coexisting rtCYE/rtCYEI and lamivudine-resistance mutations,and 2 patients had coexisting rtCYE/rtCYEI and adefovir-resistance mutations.Compared with the wild-type(WT)strain,the replication capacity of rtCYE/rtCYEI mutants from representative patients decreased by 41.1%-71.8%,and TDF susceptibility reduced by less than 2-fold,but rtCYEI+rtA181V/N236T mutants exhibited a 6.2-/9.9-fold decrease in TDF susceptibility.Molecular modeling showed that rtCYE/rtCYEI mutants had a slight decrease in binding energy to TDF compared to the WT strain.In the clinic,emergence of the rtCYE/rtCYEI mutations was not specifically associated with TDF treatment.CONCLUSION HBV rtCYE/rtCYEI mutations have a limited effect on TDF susceptibility and are not sufficient to cause TDF resistance.展开更多
Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progres...Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progressive disease deterioration and increased severity.Nevertheless,the molecular mechanisms underlying dengue-associated neutropenia remain inadequately elucidated.In this study,the comprehensive plasma proteomic profiling of dengue fever(DF)patients,DF patients with neutropenia(DFN),and healthy controls(HC)was systematically analyzed using a deep dataindependent acquisition(DIA)workflow combined with LC-MS/MS analysis,to elucidate key cellular pathways and identify promising biomarkers.DFN patients exhibited significant dual hematological alterations,with notable changes in both platelet and neutrophil counts,reflecting a complex disturbance in hematological homeostasis during dengue progression.DIA analysis quantified 2475 proteins,revealing widespread proteomic alterations among the DF,DFN,and HC subjects.Differential analysis highlighted significant fluctuations in proteins related to cytoskeletal organization,metabolic regulation,and intracellular signaling.Enrichment analyses implicated pathways such as focal adhesion,platelet activation,and PI3K-Akt signaling.Machine learning methods further identified a panel of four biomarkers-CNST,DSTN,DUSP3,and PDIA5-with high predictive accuracy for dengue diagnosis and subgroup differentiation.In conclusion,this study advances our understanding of dengue’s plasma proteomic landscape and underscores the synergistic potential of DIA-based proteomics and machine learning in unveiling host-response mechanisms,thereby informing early diagnosis and targeted therapeutic strategies.展开更多
BACKGROUND Non-invasive evaluation for liver fibrosis is clinically important,especially in patients with undetectable hepatitis B virus(HBV)DNA treated with nucleoside analogs.AIM To clarify the monitoring power of h...BACKGROUND Non-invasive evaluation for liver fibrosis is clinically important,especially in patients with undetectable hepatitis B virus(HBV)DNA treated with nucleoside analogs.AIM To clarify the monitoring power of hepatitis B core-related antigen(HBcrAg)for hepatic histologic changes in patients with chronic hepatitis B(CHB)treated with entecavir.METHODS This prospective multicenter study used multiple ordinal and multivariate logistics regression analysis to assess variables associated with Ishak fibrosis score and regression for fibrosis regression,respectively,in 403 CHB patients,including 374 with entecavir for 72 weeks(291 underwent paired liver biopsy)and 29 as controls.RESULTS Level of HBcrAg correlated negatively with liver fibrosis staging(γ=-0.357,P<0.001)in hepatitis B e antigen(HBeAg)-positive patients,and positively with liver fibrosis staging in HBeAg-negative patients.Higher HBcrAg concentration was associated with younger age,HBeAg positive status,high HBV DNA loads,high level of hepatitis B surface antigen(HBsAg)and higher necroinflammation,but not with HBV genotype.Serum concentration of HBcrAg,basal core promoter/precore(BCP/PC)mutant,quantitation of HBsAg(qHBsAg)and platelet counts were independently associated with Ishak fibrosis score on multiple ordinal regression.HBV DNA was undetectable in 88.37%of patients treated with entecavir at week 72,while their level of HBcrAg was still detectable.A greater reduction in post-treatment HBcrAg concentration was associated with the regression of hepatic fibrosis and histological improvement.HBcrAg concentration>6.33 log IU/mL at baseline and logarithmic reduction>1.03 log IU/mL at week 72 were associated with a higher chance of regression of liver fibrosis and histological improvement,respectively.CONCLUSION HBcrAg level is associated with liver fibrosis progression.HBcrAg is an excellent monitor of hepatic histological changes,especially in CHB patients treated with nucleoside analogs.展开更多
Ternary lithium-ion batteries(LIBs),widely used in new energy vehicles and electronic products,are known for their high en-ergy density,wide operating temperature range,and excellent cycling performance.With the rapid...Ternary lithium-ion batteries(LIBs),widely used in new energy vehicles and electronic products,are known for their high en-ergy density,wide operating temperature range,and excellent cycling performance.With the rapid development of the battery industry,the recycling of spent ternary LIBs has become a hot topic because of their economic value and environmental concerns.To date,a con-siderable amount of literature has reported on the recycling of spent ternary LIBs designed to provide an efficient,economical,and envir-onmentally friendly method for battery recycling.This article examines the latest developments in various technologies for recycling spent ternary LIBs in both research and practical production,including pretreatment,pyrometallurgy,hydrometallurgy,pyro-hydrometallurgy,and direct regeneration.Suggestions for addressing challenges based on the benefits and disadvantages of each method are made.Finally,through a comparison of the feasibility and economic benefits of various technologies,the challenges faced during battery recycling are summarized,and future development directions are proposed.展开更多
We build holographic p-wave conductor(insulator)/superconductor models via the numerical method with a new form of Weyl coupling in five-dimensional Lifshitz gravity,and then investigate how the Weyl coupling paramete...We build holographic p-wave conductor(insulator)/superconductor models via the numerical method with a new form of Weyl coupling in five-dimensional Lifshitz gravity,and then investigate how the Weyl coupling parameterγand the Lifshitz scaling parameter z affect the superconductor models.In the conductor/superconductor model,an increase in the Weyl correction(Lifshitz scaling)enhances(inhibits)the superconductor phase transition.Meanwhile,both the Weyl correction(when the Lifshitz parameter is large enough and fixed)and the Lifshitz scaling suppress the growth of the real part of the conductivity.The Weyl correction used here(CB^(2))shows weaker effects on the critical value than the previous Weyl correction(CF^(2)).In the insulator/superconductor model,larger vaules of the Weyl parameter hinder the formation of condensate.However,in increase in the Lifshitz scaling enhances the appearance of condensate.In addition,the calculation suggests that a competitive relation may exist between the Weyl correction and the Lifshitz scaling.展开更多
Purpose:Intertrochanteric fractures undergoing proximal femoral nail antirotation(PFNA)surgery are associated with significant hidden blood loss.This study aimed to explore whether intramedullary administration of tra...Purpose:Intertrochanteric fractures undergoing proximal femoral nail antirotation(PFNA)surgery are associated with significant hidden blood loss.This study aimed to explore whether intramedullary administration of tranexamic acid(TXA)can reduce bleeding in PFNA surgery for intertrochanteric fractures in elderly individuals.Methods:A randomized controlled trial was conducted from January 2019 to December 2022.Patients aged over 60 years with intertrochanteric fractures who underwent intramedullary fixation surgery with PFNA were eligible for inclusion and grouped according to random numbers.A total of 249 patients were initially enrolled,of which 83 were randomly allocated to the TXA group and 82 were allocated to the saline group.The TXA group received intramedullary perfusion of TXA after the bone marrow was reamed.The primary outcomes were total peri-operative blood loss and post-operative transfusion rate.The occurrence of adverse events was also recorded.Continuous data was analyzed by unpaired t-test or Mann-Whitney U test,and categorical data was analyzed by Pearson Chi-square test.Results:The total peri-operative blood loss(mL)in the TXA group was significantly lower than that in the saline group(577.23±358.02 vs.716.89±420.30,p=0.031).The post-operative transfusion rate was 30.67%in the TXA group and 47.95%in the saline group(p=0.031).The extent of post-operative deep venous thrombosis and the 3-month mortality rate were similar between the 2 groups.Conclusion:We observed that intramedullary administration of TXA in PFNA surgery for intertrochanteric fractures in elderly individuals resulted in less peri-operative blood loss and decreased transfusion rate,without any adverse effects,and is,thus,recommended.展开更多
Microbes play a crucial ecological role in soils,but the presence of relic DNA left by previous microorganisms can lead to inaccurate estimations of viable microbial function and diversity.To address this,we proposed ...Microbes play a crucial ecological role in soils,but the presence of relic DNA left by previous microorganisms can lead to inaccurate estimations of viable microbial function and diversity.To address this,we proposed a new method for removing relic DNA in soil using Benzonase endonuclease and compared it with propidium monoazide(PMA)and DNase I,which have been widely applied in viable microbiome studies.Unlike PMA,Benzonase does not require light activation and is suitable for use in opaque media such as soil.Therefore,its efficiency(40%-60%)in removing soil relic DNA was twice that of PMA(0-30%).Moreover,our results showed that Benzonase outperformed DNase I in most soils,probably due to its broader range of operating conditions compared to DNase I.In addition to higher relic DNA removal efficiency,Benzonase exhibited a weak impact on soil viable microbial communities.Subsequently,Benzonase was used to remove relic DNA in natural soils,and the results showed that relic DNA removal led to an approximately 10%reduction in microbial diversity and richness on average.Notably,it caused significant changes in the relative abundance of specific taxa,such as Bacillus and Sphingomonas.These findings reveal disparities between total and viable microbiomes in soils.Our study not only provides a reliable method for soil relic DNA removal but also highlights the necessity of relic DNA removal for viable soil microbiome assessments,laying the methodological foundation for advancing soil microbial ecology research.展开更多
Due to over-abundant information on the Web, information filtering becomes a key task for online users to obtain relevant suggestions and how to extract the most related item is always a key topic for researchers in v...Due to over-abundant information on the Web, information filtering becomes a key task for online users to obtain relevant suggestions and how to extract the most related item is always a key topic for researchers in various fields. In this paper, we adopt tools used to analyze complex networks to evaluate user reputation and item quality. In our proposed Accumulative Time Based Ranking (ATR) algorithm, we take into account the growth record of the network to identify the evolution of the reputation of users and the quality of items, by incorporating two behavior weighting factors which can capture the hidden facts on reputation and quality dynamics for each user and item respectively. Our proposed ATR algorithm mainly combines the iterative approach to rank user reputation and item quality with temporal dependence compared with other reputation evaluation methods. We show that our algorithm outperforms other benchmark ranking algorithms in terms of precision and robustness on empirical datasets from various online retailers and the citation datasets among research publications. Therefore, our proposed method has the capability to effectively evaluate user reputation and item quality.展开更多
In this Letter, an alternative solution is proposed and demonstrated for simultaneous measurement of axial strain and temperature. This sensor consists of two twisted points on a commercial single mode fiber introduce...In this Letter, an alternative solution is proposed and demonstrated for simultaneous measurement of axial strain and temperature. This sensor consists of two twisted points on a commercial single mode fiber introduced by flame-heated and rotation treatment. The fabrication process modifies the geometrical configuration and refractive index of the fiber. Different cladding modes are excited at the first twisted point, and part of them are coupled back to the fiber core at the second twisted point. Experimental results show distinct sensitivities of 34.9 pm/με with 49.23 pm/℃ and -36.19 pm/με with 62.99 pm/℃ for the two selected destructive interference wavelengths.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos.12475012,62461160263 for P.W.,and 62276171 for H.L.)Quantum Science and Technology-National Science and Technology Major Project of China (Project No.2023ZD0300600 for P.W.)+3 种基金Guangdong Provincial Quantum Science Strategic Initiative (Grant Nos.GDZX240-3009 and GDZX2303005 for P.W.)Guangdong Basic and Applied Basic Research Foundation (Grant No.2024-A1515011938 for H.L.)Shenzhen Fundamental ResearchGeneral Project (Grant No.JCYJ20240813141503005 for H.L.)the Talents Introduction Foundation of Beijing Normal University (Grant No.310432106 for P.W.)。
文摘Hyperpolarization of nuclear spins is crucial for advancing nuclear magnetic resonance and quantum information technologies,as nuclear spins typically exhibit extremely low polarization at room temperature due to their small gyromagnetic ratios.A promising approach to achieving high nuclear spin polarization is transferring the polarization of electrons to nuclear spins.The nitrogen-vacancy(NV)center in diamond has emerged as a highly effective medium for this purpose,and various hyperpolarization protocols have been developed.Among these,the pulsed polarization(PulsePol)method has been extensively studied due to its robustness against static energy shifts of the electron spin.In this work,we present a novel polarization protocol and uncover a family of magic sequences for hyperpolarizing nuclear spins,with PulsePol emerging as a special case of our general approach.Notably,we demonstrate that some of these magic sequences exhibit significantly greater robustness compared to the PulsePol protocol in the presence of finite half𝜋pulse duration of the protocol,Rabi and detuning errors.This enhanced robustness positions our protocol as a more suitable candidate for hyper-polarizing nuclear spins species with large gyromagnetic ratios and also ensures better compatibility with high-efficiency readout techniques at high magnetic fields.Additionally,the generality of our protocol allows for its direct application to other solid-state quantum systems beyond the NV center.
基金Supported by The National Natural Science Foundation of China,No.82470632.
文摘BACKGROUND Whether rtS106C+H126Y+D134E/rtS106C+H126Y+D134E+L269I(rtCYE/rtCYEI)mutations in the hepatitis B virus(HBV)reverse-transcriptase(RT)region are associated with tenofovir disoproxil fumarate(TDF)resistance is controversial.AIM To evaluate the presence of the rtCYE/rtCYEI mutations in a large cohort of Chinese patients with chronic HBV infection.METHODS A total of 28236 patients who underwent drug resistance testing at the Fifth Medical Center of Chinese PLA General Hospital from 2007 to 2019 were enrolled.All patients received nucleoside/nucleotide analogues(NAs)therapy,and serum samples were collected for sequence analysis of the HBV RT domain with mutation analysis.RESULTS The detection rates of a single mutation of rtS106C,rtH126Y,rtD134E,and rtL269I were 8.21%,3.20%,2.55%and 61.49%in 23718 genotype C patients,and 1.31%,1.76%,0.21%,and 92.33%in 4266 genotype B patients,respectively.The combined mutations of rtCYE/rtCYEI were only detected in 12 genotype C patients,accounting for 0.042%of all patients.These 12 patients had received NA treatments except TDF before testing.Among them,6 patients had coexisting rtCYE/rtCYEI and lamivudine-resistance mutations,and 2 patients had coexisting rtCYE/rtCYEI and adefovir-resistance mutations.Compared with the wild-type(WT)strain,the replication capacity of rtCYE/rtCYEI mutants from representative patients decreased by 41.1%-71.8%,and TDF susceptibility reduced by less than 2-fold,but rtCYEI+rtA181V/N236T mutants exhibited a 6.2-/9.9-fold decrease in TDF susceptibility.Molecular modeling showed that rtCYE/rtCYEI mutants had a slight decrease in binding energy to TDF compared to the WT strain.In the clinic,emergence of the rtCYE/rtCYEI mutations was not specifically associated with TDF treatment.CONCLUSION HBV rtCYE/rtCYEI mutations have a limited effect on TDF susceptibility and are not sufficient to cause TDF resistance.
基金supported by National Key R&D Program of China(2023YFA0915600)Guangdong Basic and Applied Basic Research Foundation(2025B1515020010)+3 种基金Shenzhen Clinical Research Center for Emerging Infectious Diseases(LCYSSQ20220823091203007)Sanming Project of Medicine in Shenzhen(SZSM202311033)Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties(No.SZGSP011)Shenzhen High-level Hospital Construction Fund(XKJSCRGRK-006).
文摘Dengue virus(DENV)remains a pervasive global health threat,further complicated by the occurrence of neutropenia-a distinct clinical feature indicative of an altered host immune response,closely correlated with progressive disease deterioration and increased severity.Nevertheless,the molecular mechanisms underlying dengue-associated neutropenia remain inadequately elucidated.In this study,the comprehensive plasma proteomic profiling of dengue fever(DF)patients,DF patients with neutropenia(DFN),and healthy controls(HC)was systematically analyzed using a deep dataindependent acquisition(DIA)workflow combined with LC-MS/MS analysis,to elucidate key cellular pathways and identify promising biomarkers.DFN patients exhibited significant dual hematological alterations,with notable changes in both platelet and neutrophil counts,reflecting a complex disturbance in hematological homeostasis during dengue progression.DIA analysis quantified 2475 proteins,revealing widespread proteomic alterations among the DF,DFN,and HC subjects.Differential analysis highlighted significant fluctuations in proteins related to cytoskeletal organization,metabolic regulation,and intracellular signaling.Enrichment analyses implicated pathways such as focal adhesion,platelet activation,and PI3K-Akt signaling.Machine learning methods further identified a panel of four biomarkers-CNST,DSTN,DUSP3,and PDIA5-with high predictive accuracy for dengue diagnosis and subgroup differentiation.In conclusion,this study advances our understanding of dengue’s plasma proteomic landscape and underscores the synergistic potential of DIA-based proteomics and machine learning in unveiling host-response mechanisms,thereby informing early diagnosis and targeted therapeutic strategies.
基金Supported by Chinese Ministry of Science and Technology Grants the Major Science and Technology Special Project Fund Scheme,No.2013ZX10005002Beijing the Special Clinical Application Research and Translational Grants,No.Z151100004015221
文摘BACKGROUND Non-invasive evaluation for liver fibrosis is clinically important,especially in patients with undetectable hepatitis B virus(HBV)DNA treated with nucleoside analogs.AIM To clarify the monitoring power of hepatitis B core-related antigen(HBcrAg)for hepatic histologic changes in patients with chronic hepatitis B(CHB)treated with entecavir.METHODS This prospective multicenter study used multiple ordinal and multivariate logistics regression analysis to assess variables associated with Ishak fibrosis score and regression for fibrosis regression,respectively,in 403 CHB patients,including 374 with entecavir for 72 weeks(291 underwent paired liver biopsy)and 29 as controls.RESULTS Level of HBcrAg correlated negatively with liver fibrosis staging(γ=-0.357,P<0.001)in hepatitis B e antigen(HBeAg)-positive patients,and positively with liver fibrosis staging in HBeAg-negative patients.Higher HBcrAg concentration was associated with younger age,HBeAg positive status,high HBV DNA loads,high level of hepatitis B surface antigen(HBsAg)and higher necroinflammation,but not with HBV genotype.Serum concentration of HBcrAg,basal core promoter/precore(BCP/PC)mutant,quantitation of HBsAg(qHBsAg)and platelet counts were independently associated with Ishak fibrosis score on multiple ordinal regression.HBV DNA was undetectable in 88.37%of patients treated with entecavir at week 72,while their level of HBcrAg was still detectable.A greater reduction in post-treatment HBcrAg concentration was associated with the regression of hepatic fibrosis and histological improvement.HBcrAg concentration>6.33 log IU/mL at baseline and logarithmic reduction>1.03 log IU/mL at week 72 were associated with a higher chance of regression of liver fibrosis and histological improvement,respectively.CONCLUSION HBcrAg level is associated with liver fibrosis progression.HBcrAg is an excellent monitor of hepatic histological changes,especially in CHB patients treated with nucleoside analogs.
基金sponsored by the National Natural Science Foundation of China(Nos.52204412 and U2002212)the National Key R&D Program of China(No.2021YFC 1910504)the Fundamental Research Funds for the Central Universities(No.FRF-TP-20-031A1).
文摘Ternary lithium-ion batteries(LIBs),widely used in new energy vehicles and electronic products,are known for their high en-ergy density,wide operating temperature range,and excellent cycling performance.With the rapid development of the battery industry,the recycling of spent ternary LIBs has become a hot topic because of their economic value and environmental concerns.To date,a con-siderable amount of literature has reported on the recycling of spent ternary LIBs designed to provide an efficient,economical,and envir-onmentally friendly method for battery recycling.This article examines the latest developments in various technologies for recycling spent ternary LIBs in both research and practical production,including pretreatment,pyrometallurgy,hydrometallurgy,pyro-hydrometallurgy,and direct regeneration.Suggestions for addressing challenges based on the benefits and disadvantages of each method are made.Finally,through a comparison of the feasibility and economic benefits of various technologies,the challenges faced during battery recycling are summarized,and future development directions are proposed.
基金supported by the National Natural Science Foundation of China(6113900261501229+1 种基金11547040)the Guangdong Natural Science Foundation(2016A030310051)
基金supported in part by NSFC(Grant Nos.11865012,12075109,12075143 and 11747615)the Foundation of Scientific Innovative Research Team of Education Department of Guizhou Province(201329)。
文摘We build holographic p-wave conductor(insulator)/superconductor models via the numerical method with a new form of Weyl coupling in five-dimensional Lifshitz gravity,and then investigate how the Weyl coupling parameterγand the Lifshitz scaling parameter z affect the superconductor models.In the conductor/superconductor model,an increase in the Weyl correction(Lifshitz scaling)enhances(inhibits)the superconductor phase transition.Meanwhile,both the Weyl correction(when the Lifshitz parameter is large enough and fixed)and the Lifshitz scaling suppress the growth of the real part of the conductivity.The Weyl correction used here(CB^(2))shows weaker effects on the critical value than the previous Weyl correction(CF^(2)).In the insulator/superconductor model,larger vaules of the Weyl parameter hinder the formation of condensate.However,in increase in the Lifshitz scaling enhances the appearance of condensate.In addition,the calculation suggests that a competitive relation may exist between the Weyl correction and the Lifshitz scaling.
基金funded by Hengyang Science and Technology Bureau(2019jh011135)Hengyang Central Hospital.This work was supported by Health Research Project of Hunan Provincial Health Commission(grant number D202304077906)Hengyang Science and Technology Bureau(grant number 2019jh011135).
文摘Purpose:Intertrochanteric fractures undergoing proximal femoral nail antirotation(PFNA)surgery are associated with significant hidden blood loss.This study aimed to explore whether intramedullary administration of tranexamic acid(TXA)can reduce bleeding in PFNA surgery for intertrochanteric fractures in elderly individuals.Methods:A randomized controlled trial was conducted from January 2019 to December 2022.Patients aged over 60 years with intertrochanteric fractures who underwent intramedullary fixation surgery with PFNA were eligible for inclusion and grouped according to random numbers.A total of 249 patients were initially enrolled,of which 83 were randomly allocated to the TXA group and 82 were allocated to the saline group.The TXA group received intramedullary perfusion of TXA after the bone marrow was reamed.The primary outcomes were total peri-operative blood loss and post-operative transfusion rate.The occurrence of adverse events was also recorded.Continuous data was analyzed by unpaired t-test or Mann-Whitney U test,and categorical data was analyzed by Pearson Chi-square test.Results:The total peri-operative blood loss(mL)in the TXA group was significantly lower than that in the saline group(577.23±358.02 vs.716.89±420.30,p=0.031).The post-operative transfusion rate was 30.67%in the TXA group and 47.95%in the saline group(p=0.031).The extent of post-operative deep venous thrombosis and the 3-month mortality rate were similar between the 2 groups.Conclusion:We observed that intramedullary administration of TXA in PFNA surgery for intertrochanteric fractures in elderly individuals resulted in less peri-operative blood loss and decreased transfusion rate,without any adverse effects,and is,thus,recommended.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.32100094 to Y.Wang and 42020104003 to Q.Huang)。
文摘Microbes play a crucial ecological role in soils,but the presence of relic DNA left by previous microorganisms can lead to inaccurate estimations of viable microbial function and diversity.To address this,we proposed a new method for removing relic DNA in soil using Benzonase endonuclease and compared it with propidium monoazide(PMA)and DNase I,which have been widely applied in viable microbiome studies.Unlike PMA,Benzonase does not require light activation and is suitable for use in opaque media such as soil.Therefore,its efficiency(40%-60%)in removing soil relic DNA was twice that of PMA(0-30%).Moreover,our results showed that Benzonase outperformed DNase I in most soils,probably due to its broader range of operating conditions compared to DNase I.In addition to higher relic DNA removal efficiency,Benzonase exhibited a weak impact on soil viable microbial communities.Subsequently,Benzonase was used to remove relic DNA in natural soils,and the results showed that relic DNA removal led to an approximately 10%reduction in microbial diversity and richness on average.Notably,it caused significant changes in the relative abundance of specific taxa,such as Bacillus and Sphingomonas.These findings reveal disparities between total and viable microbiomes in soils.Our study not only provides a reliable method for soil relic DNA removal but also highlights the necessity of relic DNA removal for viable soil microbiome assessments,laying the methodological foundation for advancing soil microbial ecology research.
基金This work was supported by the National Natural Science Foundation of China under Grant No.61803266the Natural Science Foundation of Guangdong Province of China under Grant Nos.2019A1515011173 and 2019A1515011064+2 种基金the Shenzhen Fundamental Research-General Project under Grant No.JCYJ20190808162601658the Research Grants Council of the Hong Kong Special Administrative Region,China,under Grant Nos.GRF 18304316,GRF 18301217 and GRF 18301119the Dean's Research Fund of the Faculty of Liberal Arts and Social Sciences,The Education University of Hong Kong,Hong Kong Special Administrative Region,China,under Grant No.FLASS/DRF 04418,and the CCF-Baidu Open Fund.
文摘Due to over-abundant information on the Web, information filtering becomes a key task for online users to obtain relevant suggestions and how to extract the most related item is always a key topic for researchers in various fields. In this paper, we adopt tools used to analyze complex networks to evaluate user reputation and item quality. In our proposed Accumulative Time Based Ranking (ATR) algorithm, we take into account the growth record of the network to identify the evolution of the reputation of users and the quality of items, by incorporating two behavior weighting factors which can capture the hidden facts on reputation and quality dynamics for each user and item respectively. Our proposed ATR algorithm mainly combines the iterative approach to rank user reputation and item quality with temporal dependence compared with other reputation evaluation methods. We show that our algorithm outperforms other benchmark ranking algorithms in terms of precision and robustness on empirical datasets from various online retailers and the citation datasets among research publications. Therefore, our proposed method has the capability to effectively evaluate user reputation and item quality.
基金supported by the National Natural Science Foundation of China(Nos.61775070 and 61275083)the Fundamental Research Funds for the Central Universities(No.2017KFYXJJ032)
文摘In this Letter, an alternative solution is proposed and demonstrated for simultaneous measurement of axial strain and temperature. This sensor consists of two twisted points on a commercial single mode fiber introduced by flame-heated and rotation treatment. The fabrication process modifies the geometrical configuration and refractive index of the fiber. Different cladding modes are excited at the first twisted point, and part of them are coupled back to the fiber core at the second twisted point. Experimental results show distinct sensitivities of 34.9 pm/με with 49.23 pm/℃ and -36.19 pm/με with 62.99 pm/℃ for the two selected destructive interference wavelengths.
