Adult tissue-derived organoids allow for the expansion and maintenance of primary epithelial cells in a near-native state.These 3D and self-organizing organotypic cultures derived from adult tissues have been increasi...Adult tissue-derived organoids allow for the expansion and maintenance of primary epithelial cells in a near-native state.These 3D and self-organizing organotypic cultures derived from adult tissues have been increasingly used in fundamental and translational research.A key feature of this organoid system is that it recapitulates the stem cell lineage and thus,the differentiated cell-type heterogeneity of the in vivo tissue of origin.展开更多
The gastrointestinal(GI)tract's primary role is food digestion,relying on coordinated fluid secretion and bowel movements triggered by mechanosensation.Enteroendocrine cells(EECs)are specialized mechanosensitive c...The gastrointestinal(GI)tract's primary role is food digestion,relying on coordinated fluid secretion and bowel movements triggered by mechanosensation.Enteroendocrine cells(EECs)are specialized mechanosensitive cells that convert mechanical forces into electrochemical signals,culminating in serotonin release to regulate GI motility.Despite their pivotal role,knowledge of EEC mechanical properties has been lacking due to their rarity and limited accessibility.In this brief report,we present the first single-cell mechanical characterization of human ECCs isolated from healthy intestinal organoids.Using single-cell optical tweezers,we measured EEC stiffness profiles at the physiological temperature and investigated changes following tryptophan metabolism inhibition.Our findings not only shed light on EEC mechanics but also highlight the potential of adult stem cell-derived organoids for studying these elusive cells.展开更多
基金This work was supported by the European Research Council under ERC Advanced Grant Agreement no.67013(H.C.and K.B.)。
文摘Adult tissue-derived organoids allow for the expansion and maintenance of primary epithelial cells in a near-native state.These 3D and self-organizing organotypic cultures derived from adult tissues have been increasingly used in fundamental and translational research.A key feature of this organoid system is that it recapitulates the stem cell lineage and thus,the differentiated cell-type heterogeneity of the in vivo tissue of origin.
文摘The gastrointestinal(GI)tract's primary role is food digestion,relying on coordinated fluid secretion and bowel movements triggered by mechanosensation.Enteroendocrine cells(EECs)are specialized mechanosensitive cells that convert mechanical forces into electrochemical signals,culminating in serotonin release to regulate GI motility.Despite their pivotal role,knowledge of EEC mechanical properties has been lacking due to their rarity and limited accessibility.In this brief report,we present the first single-cell mechanical characterization of human ECCs isolated from healthy intestinal organoids.Using single-cell optical tweezers,we measured EEC stiffness profiles at the physiological temperature and investigated changes following tryptophan metabolism inhibition.Our findings not only shed light on EEC mechanics but also highlight the potential of adult stem cell-derived organoids for studying these elusive cells.
