Viral myocarditis(VMC)is one of the most common acquired heart diseases in children and teenagers.However,its pathogenesis is still unclear,and effective treatments are lacking.This study aimed to investigate the regu...Viral myocarditis(VMC)is one of the most common acquired heart diseases in children and teenagers.However,its pathogenesis is still unclear,and effective treatments are lacking.This study aimed to investigate the regulatory pathway by which exosomes alleviate ferroptosis in cardiomyocytes(CMCs)induced by coxsackievirus B3(CVB3).CVB3 was utilized for inducing the VMC mouse model and cellular model.Cardiac echocardiography,left ventricular ejection fraction(LVEF),and left ventricular fractional shortening(LVFS)were implemented to assess the cardiac function.In CVB3-induced VMC mice,cardiac insufficiency was observed,as well as the altered levels of ferroptosis-related indicators(glutathione) peroxidase 4(GPX4),glutathione(GSH),and malondialdehyde(MDA).However,exosomes derived from human umbilical cord mesenchymal stem cells(hucMSCs-exo)could restore the changes caused by CVB3 stimulation.Let-7a-5p was enriched in hucMSCs-exo,and the inhibitory ffect of hucMSCs-exoa-ie-pmimo on CVB3-induced ferroptosis was higher than that of hucMSCs-exommie N(NC:negative control).Mothers against decapentaplegic homolog 2(SMAD2)increased in the VMC group,while the expression of zinc-finger protein 36(ZFP36)decreased.Let-7a-5p was confirmed to interact with SMAD2 messenger RNA(mRNA),and the SMAD2 protein interacted directly with the ZFP36 protein.Silencing SMAD2 and overexpressing ZFP36 inhibited the expression of ferroptosis-related indicators.Meanwhile,the levels of GPX4,solute carrier family 7,member 11(SLC7A11),and GSH were lower in the SMAD2 overexpression plasmid(oe-SMAD2)+let-7a-5p mimic group than in the oe-NC+let-7a-5p mimic group,while those of MDA,reactive oxygen species(ROS),and Fe^(2+)increased.In conclusion,these data showed that ferroptosis could be regulated by mediating SMAD2 expression.Exo-let-7a-5p derived from hucMSCs could mediate SMAD2 to promote the expression of ZFP36,which further inhibited the ferroptosis of CMCs to alleviate CVB3-induced VMC.展开更多
To the Editor:Transplacental digoxin therapy has been proven effective in converting fetal supraventricular arrhythmia(SVA)to sinus rhythm or achieving ventricular rate control,significantly improving fetal intrauteri...To the Editor:Transplacental digoxin therapy has been proven effective in converting fetal supraventricular arrhythmia(SVA)to sinus rhythm or achieving ventricular rate control,significantly improving fetal intrauterine outcomes.[1–3]However,limited data exist from large patient populations in China.Additionally,concerns remain regarding the relatively high non-response and intrauterine relapse rates during prenatal digoxin treatment.[4,5]Studies exploring the underlying risk factors for digoxin non-response and intrauterine relapse are limited.Therefore,this study aimed to analyze the Chinese data on outcomes and follow-up of fetal SVA treated with prenatal digoxin monotherapy and investigate risk factors associated with unresponsiveness and intrauterine relapse after transplacental digoxin treatment.展开更多
Introduction:An estimated of 900,000 infants are born with birth defects each year in China causing a substantial disease burden.This study aimed to depict the epidemiological patterns of selected major birth defects ...Introduction:An estimated of 900,000 infants are born with birth defects each year in China causing a substantial disease burden.This study aimed to depict the epidemiological patterns of selected major birth defects in Chinese perinatal births and provide important baseline data for future prevention.Methods:Data from the Chinese Birth Defects Monitoring Network(CBDMN)during 2010–2018 were used to analyze the epidemiological pattern in the prevalence of 15 major birth defects and the trends over time.Results:In the period of 2010–2018,the top 10 most frequently-occurring birth defects in China included congenital heart diseases(CHDs),polydactyly,cleft lip with or without palate(CL/P),club foot,syndactyly,hydrocephalus,hypospadias,limb reduction defects(LRD),anotia/microtia,and anorectal atresia/stenosis.There was a decrease in the prevalence of neural tube defects,CL/P,hydrocephalus,LRD,gastroschisis,and omphalocele,but there were increases in the prevalence of CHDs,cleft palate,hypospadias,club foot,polydactyly,and syndactyly.The prevalence of most birth defects varied significantly by maternal age,area types(urban/rural),and geographic regions.Conclusions and Implications for Public Health Practice:The findings indicated that the comprehensive prevention of birth defects should focus on these selected birth defects,elderly pregnant women,rural areas,and western regions.展开更多
Cervical cancer remains a significant global public health issue due to its high incidence and mortality.Current clinical guidelines recommend screening for high-risk human papillomavirus(hrHPV)-DNA alongside a Thinpr...Cervical cancer remains a significant global public health issue due to its high incidence and mortality.Current clinical guidelines recommend screening for high-risk human papillomavirus(hrHPV)-DNA alongside a Thinprep cytologic test(TCT)before further medical evaluation[1].展开更多
基金supported by the China Postdoctoral Science Foundation(No.2022M712252)the Natural Science Foundation of Sichuan Province,China(No.2023NSFSC1634).
文摘Viral myocarditis(VMC)is one of the most common acquired heart diseases in children and teenagers.However,its pathogenesis is still unclear,and effective treatments are lacking.This study aimed to investigate the regulatory pathway by which exosomes alleviate ferroptosis in cardiomyocytes(CMCs)induced by coxsackievirus B3(CVB3).CVB3 was utilized for inducing the VMC mouse model and cellular model.Cardiac echocardiography,left ventricular ejection fraction(LVEF),and left ventricular fractional shortening(LVFS)were implemented to assess the cardiac function.In CVB3-induced VMC mice,cardiac insufficiency was observed,as well as the altered levels of ferroptosis-related indicators(glutathione) peroxidase 4(GPX4),glutathione(GSH),and malondialdehyde(MDA).However,exosomes derived from human umbilical cord mesenchymal stem cells(hucMSCs-exo)could restore the changes caused by CVB3 stimulation.Let-7a-5p was enriched in hucMSCs-exo,and the inhibitory ffect of hucMSCs-exoa-ie-pmimo on CVB3-induced ferroptosis was higher than that of hucMSCs-exommie N(NC:negative control).Mothers against decapentaplegic homolog 2(SMAD2)increased in the VMC group,while the expression of zinc-finger protein 36(ZFP36)decreased.Let-7a-5p was confirmed to interact with SMAD2 messenger RNA(mRNA),and the SMAD2 protein interacted directly with the ZFP36 protein.Silencing SMAD2 and overexpressing ZFP36 inhibited the expression of ferroptosis-related indicators.Meanwhile,the levels of GPX4,solute carrier family 7,member 11(SLC7A11),and GSH were lower in the SMAD2 overexpression plasmid(oe-SMAD2)+let-7a-5p mimic group than in the oe-NC+let-7a-5p mimic group,while those of MDA,reactive oxygen species(ROS),and Fe^(2+)increased.In conclusion,these data showed that ferroptosis could be regulated by mediating SMAD2 expression.Exo-let-7a-5p derived from hucMSCs could mediate SMAD2 to promote the expression of ZFP36,which further inhibited the ferroptosis of CMCs to alleviate CVB3-induced VMC.
基金This work was supported by grants from the Natural Science Foundation of Sichuan Province(Nos.2024YFFK0272,2024YFFK0078,2024NSFSC1711,2025ZNSFSC1674,and 2025ZNSFSC0705)Key Research and Development Project of Chengdu Science and Technology Bureau(Nos.2024-YF05-00237-SN and 2024-Y05-00506-SN)+1 种基金National Natural Science Foundation of China(No.82370236)National Key Research and Development Program of China(No.2022YFC2703902).
文摘To the Editor:Transplacental digoxin therapy has been proven effective in converting fetal supraventricular arrhythmia(SVA)to sinus rhythm or achieving ventricular rate control,significantly improving fetal intrauterine outcomes.[1–3]However,limited data exist from large patient populations in China.Additionally,concerns remain regarding the relatively high non-response and intrauterine relapse rates during prenatal digoxin treatment.[4,5]Studies exploring the underlying risk factors for digoxin non-response and intrauterine relapse are limited.Therefore,this study aimed to analyze the Chinese data on outcomes and follow-up of fetal SVA treated with prenatal digoxin monotherapy and investigate risk factors associated with unresponsiveness and intrauterine relapse after transplacental digoxin treatment.
文摘Introduction:An estimated of 900,000 infants are born with birth defects each year in China causing a substantial disease burden.This study aimed to depict the epidemiological patterns of selected major birth defects in Chinese perinatal births and provide important baseline data for future prevention.Methods:Data from the Chinese Birth Defects Monitoring Network(CBDMN)during 2010–2018 were used to analyze the epidemiological pattern in the prevalence of 15 major birth defects and the trends over time.Results:In the period of 2010–2018,the top 10 most frequently-occurring birth defects in China included congenital heart diseases(CHDs),polydactyly,cleft lip with or without palate(CL/P),club foot,syndactyly,hydrocephalus,hypospadias,limb reduction defects(LRD),anotia/microtia,and anorectal atresia/stenosis.There was a decrease in the prevalence of neural tube defects,CL/P,hydrocephalus,LRD,gastroschisis,and omphalocele,but there were increases in the prevalence of CHDs,cleft palate,hypospadias,club foot,polydactyly,and syndactyly.The prevalence of most birth defects varied significantly by maternal age,area types(urban/rural),and geographic regions.Conclusions and Implications for Public Health Practice:The findings indicated that the comprehensive prevention of birth defects should focus on these selected birth defects,elderly pregnant women,rural areas,and western regions.
基金supported by the National Natural Science Foundation of China(82071651)Chengdu Science and Technology Bureau(2017-GH02-00030-HZ)+4 种基金National Key Research and Development Program of China(2022YFC3600304,2022YFC2704700)Science and Technology Department of Sichuan Province(23ZDYF2489)Sichuan Province Cadre Health Care Scientific Research Project(CGY2023-1701)the Chengdu Technological Innovation R&D Project(2021-YF05-00595-SN)partially sponsored by Lisen Imprinting Diagnostics,Inc.
文摘Cervical cancer remains a significant global public health issue due to its high incidence and mortality.Current clinical guidelines recommend screening for high-risk human papillomavirus(hrHPV)-DNA alongside a Thinprep cytologic test(TCT)before further medical evaluation[1].
