Serum creatinine(Scr)and blood urea nitrogen(BUN)are key biomarkers for assessing human kidney function.Nevertheless,the quantitative and simultaneous detection of these two markers with high efficiency remains a chal...Serum creatinine(Scr)and blood urea nitrogen(BUN)are key biomarkers for assessing human kidney function.Nevertheless,the quantitative and simultaneous detection of these two markers with high efficiency remains a challenge.To address this,a highly sensitive surface‐enhanced Raman scattering(SERS)substrate,composed of carbonized photoresist embedded with silver nanoparticles(C‐AZ/Ag NPs),was developed for the efficient simultaneous detection of Scr and BUN.In the experiments,AZ5214E and silver nitrate(AgNO_(3))were first mixed in acetonitrile to prepare the precursor solution for UV photolithography.Subsequently,a porous anodic aluminum oxide coated with a thin chromium film served as a mask to transfer the pattern onto the substrate through UV lithography,and the feature size was tuned by controlling the development time.After that,the patterned substrate was carbonized under an argon atmosphere to yield the final C‐AZ/Ag NPs SERS platform.Using rhodamine 6G as a Raman reporter,the optimized C‐AZ/Ag NPs substrate achieved an enhancement factor of 1.27×10^(8)and a detection limit down to 10^(−9)M.Moreover,it exhibited good spectral uniformity with a relative standard deviation of 7.59%.Finally,clinical serum samples were tested for creatinine and urea nitrogen using the C‐AZ/Ag NPs substrate and cross‐validated against colorimetric assays.It demonstrated that the developed SERS platform accurately reproduced the trends of Scr concentration,which show significant potential for future clinical applications.展开更多
Catalysts for the desulfurization of gasoline samples were synthesized via the immobilization of well-dispersed phosphotungstic acid(HPW)on Mobil compo-sition of matter-twenty-two(MWW)zeolite.Characteriza-tion results...Catalysts for the desulfurization of gasoline samples were synthesized via the immobilization of well-dispersed phosphotungstic acid(HPW)on Mobil compo-sition of matter-twenty-two(MWW)zeolite.Characteriza-tion results indicated that these catalysts possess a mesoporous structure with the retention of the Keggin structure of immobilized HPW.Relevant reaction para-meters influencing sulfur removal were systematically investigated,including HPW loading,catalyst dosage,temperature,initial S-concentration,molar ratio of oxidant to sulfide(O/S),volume ratio of MeCN to model oil(Ext./oil),and sulfide species.The 40 wt-%HPW/MWW catalyst exhibited the highest catalytic activity with 99.6%dibenzothiophene sulfur removal from prepared samples.The 40 wt-%HPW/MWW catalyst was recycled four times and could be easily regenerated.Finally,as an exploratory study,straight-run-gasoline and fluid catalytic cracking gasoline were employed to accurately evaluate the desulfurization performance of 40 wt-%HPW/MWW.Our research provides new insights into the development and application of catalysts for desulfurization of gasoline.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:62374097Medical Science and Technology Project of Zhejiang Provincial Health Commission,Grant/Award Number:2022KY299。
文摘Serum creatinine(Scr)and blood urea nitrogen(BUN)are key biomarkers for assessing human kidney function.Nevertheless,the quantitative and simultaneous detection of these two markers with high efficiency remains a challenge.To address this,a highly sensitive surface‐enhanced Raman scattering(SERS)substrate,composed of carbonized photoresist embedded with silver nanoparticles(C‐AZ/Ag NPs),was developed for the efficient simultaneous detection of Scr and BUN.In the experiments,AZ5214E and silver nitrate(AgNO_(3))were first mixed in acetonitrile to prepare the precursor solution for UV photolithography.Subsequently,a porous anodic aluminum oxide coated with a thin chromium film served as a mask to transfer the pattern onto the substrate through UV lithography,and the feature size was tuned by controlling the development time.After that,the patterned substrate was carbonized under an argon atmosphere to yield the final C‐AZ/Ag NPs SERS platform.Using rhodamine 6G as a Raman reporter,the optimized C‐AZ/Ag NPs substrate achieved an enhancement factor of 1.27×10^(8)and a detection limit down to 10^(−9)M.Moreover,it exhibited good spectral uniformity with a relative standard deviation of 7.59%.Finally,clinical serum samples were tested for creatinine and urea nitrogen using the C‐AZ/Ag NPs substrate and cross‐validated against colorimetric assays.It demonstrated that the developed SERS platform accurately reproduced the trends of Scr concentration,which show significant potential for future clinical applications.
基金supported by Petroleum Technology Development Fund(PTDF),Nigeria,and the Training Program for Outstanding Young Teachers in Universities in Guangdong Province(Grant No.YQ2015116)Petrochemical Industry Transformation and Upgrad-ing Technology Innovation Public Service Platform in Maomning City(No.2016B020211002)+1 种基金the National Natural Science Foundation of China(Grant No.21403038)the Natural Science Foundation of Guangdong Province(Grant No.2015A030313892).
文摘Catalysts for the desulfurization of gasoline samples were synthesized via the immobilization of well-dispersed phosphotungstic acid(HPW)on Mobil compo-sition of matter-twenty-two(MWW)zeolite.Characteriza-tion results indicated that these catalysts possess a mesoporous structure with the retention of the Keggin structure of immobilized HPW.Relevant reaction para-meters influencing sulfur removal were systematically investigated,including HPW loading,catalyst dosage,temperature,initial S-concentration,molar ratio of oxidant to sulfide(O/S),volume ratio of MeCN to model oil(Ext./oil),and sulfide species.The 40 wt-%HPW/MWW catalyst exhibited the highest catalytic activity with 99.6%dibenzothiophene sulfur removal from prepared samples.The 40 wt-%HPW/MWW catalyst was recycled four times and could be easily regenerated.Finally,as an exploratory study,straight-run-gasoline and fluid catalytic cracking gasoline were employed to accurately evaluate the desulfurization performance of 40 wt-%HPW/MWW.Our research provides new insights into the development and application of catalysts for desulfurization of gasoline.
