In recent years,organic/polymer photothermal nanoagents including semiconducting polymer nanoparticles and small-molecule organic photothermal agents-encapsulated nanoparticles have attracted large attention from rese...In recent years,organic/polymer photothermal nanoagents including semiconducting polymer nanoparticles and small-molecule organic photothermal agents-encapsulated nanoparticles have attracted large attention from researchers in the biomedical field,owing to their excellent optical properties,good biocompatibility,easy processability,and flexible surface functionalization,as well as their combined functions of photoacoustic(PA)imaging and photothermal therapy(PTT).In this review,we summarize the recent advances in organic/polymer photothermal nanoagents for in vivo PA imaging and PTT applications.In particular,we focus on the design strategies,which are composed of traditional approaches and emerging mechanisms,especially based on'intramolecular motion-induced photothermy'strategy to regulate the photophysical properties of organic/polymer photothermal nanoagents for boosted in vivo PA imaging and PTT.展开更多
Semiconducting polymers(SPs)with intensive near-infrared(NIR)absorption and high photothermal conversion efficiencies have been employed as a new generation of photothermal agents(PTAs)for“all-in-one”theranostic nan...Semiconducting polymers(SPs)with intensive near-infrared(NIR)absorption and high photothermal conversion efficiencies have been employed as a new generation of photothermal agents(PTAs)for“all-in-one”theranostic nanoplatforms with integrated photoacoustic imaging(PAI)and photothermal therapy(PTT)functions.However,the lack of facile molecular design principles impedes the development of highly efficient NIR PTAs.Herein,a facile molecular design strategy based on largeπ-extended donor-acceptor(L-π-D-A)structure is reported for achieving SPs(SP1-SP3)with highly efficient in vitro and in vivo PAI and PTT capabilities.Through adjusting the conjugation length and planarity of the donor units,both SP3 and corresponding nanoparticle(SPN)SPN3 exhibit stronger D-A strength,intensive NIR absorption,enhanced absorption coefficient,and higher photothermal conversion efficiency(up to 61.8%).The excellent photothermal conversion efficiencies make SPN1-SPN3 produce efficient inhibition of tumor growth with excellent biocompatibility and prominent PAI performance with a high contrast manner in living mice at a low systemic injection mass.Our research highlights that the new L-π-D-A molecular design is an effective strategy to obtain highly efficient polymeric NIR PTAs for high desirable cancer phototheranostic nanoplatforms.展开更多
The research in the biomedical applications of aggregation? induced emission luminogens (AIEgens) has attracted increasing interest in recent years, as AIEgens have been widely accepted as promising materials for cons...The research in the biomedical applications of aggregation? induced emission luminogens (AIEgens) has attracted increasing interest in recent years, as AIEgens have been widely accepted as promising materials for constructing new generation of phototheranostic agents [1-5]. Different from conventional organic dyes, the AIEgens often have threedimensional (3D) molecular structure and contain a number of intramolecular motion units [6-8]. In the aggregates, the 3D molecular structure results in significantly reduced intermolecular interaction such as π-π stacking as compared to planar structure.展开更多
The combination of immunogenic cell death(ICD)induction and immune checkpoint blockade has emerged as a major direction of cancer immunotherapy.Among currently available ICD inducers,sonosensitizers that produce react...The combination of immunogenic cell death(ICD)induction and immune checkpoint blockade has emerged as a major direction of cancer immunotherapy.Among currently available ICD inducers,sonosensitizers that produce reactive oxygen species(ROS)under an external trigger to evoke ICD of tumor cells have shown great promise.However,a highly efficient sonosensitizer-based ICD inducer with an aggregationinduced emission(AIE)characteristic has yet to be developed.Herein,a novel AIE sonosensitizer with a twisted molecular structure,very small energy gap between the singlet and triplet excited states(ΔE_(ST)),and efficient ROS generation ability,which can serve as an effective ICD inducer,is reported for sonodynamic processes in cancer immunotherapy.Furthermore,an AIE sonosensitizer-based nanosystem with surface modification of anti-PD-L1 peptide is constructed for boosting antitumor immunotherapy.In this system,AIE sonosensitizer-mediated sonodynamic therapy can successfully convert a hypoimmunogenic cold tumor to a hot one and further facilitate the multivalent blocking of programed death ligand(PD-L1)by anti-PD-L1 peptides.Such an advanced nanosystem could effectively initiate the activation of antitumoral immune reactions and modulation of an immunosuppressive microenvironment,contributing to systemic antitumor effects to further inhibit the growth of distant tumors.展开更多
Phototheranostics that concurrently and complementarily integrate real-time diagnosis and in situ therapeutic capabilities in one platform has become the advancing edge of precision medicine.Organic agents possess the...Phototheranostics that concurrently and complementarily integrate real-time diagnosis and in situ therapeutic capabilities in one platform has become the advancing edge of precision medicine.Organic agents possess the merits of facile preparation,high purity,tunable photophysical property,good biocompatibility,and potential biodegradability,which have shown great promise for disease theranostics.This review summarizes the recent achievements of organic phototheranostic agents and applications,especially which rationally utilize energy dissipation pathways of Jablonski diagram to modulate the fluorescence emission,photoacoustic/photothermal production,and photodynamic processes.Of particular interest are the systems exhibiting huge differences in aggregate state as compared with the solution or single molecule form,during which the intramolecular motions play an important role in regulating the photophysical properties.The recent advances from such an aspect for biomedical applications including high-resolution imaging,activatable imaging and therapy,adaptive theranostics,image-guided surgery,immunotherapy,and afterglow imaging are discussed.A brief summary and perspective in this field are also presented.We hope this review will be helpful to the researchers interested in bioprobe design and theranostic applications,and inspire new insights into the linkage between aggregate science and biomedical field.展开更多
Aggregation-induced emission(AIE)nanoparticles(NPs)are widely used for image-guided tumor resection because of their high signal-to-noise ratios and long systemic circulation time.These NPs are derived by encapsulatin...Aggregation-induced emission(AIE)nanoparticles(NPs)are widely used for image-guided tumor resection because of their high signal-to-noise ratios and long systemic circulation time.These NPs are derived by encapsulating small-molecule fluorescent dyes with AIE property inside the cores of NPs assembled by amphiphilic polymers.Although the systemic circulation of AIE NPs is prolonged,hydrophilic polymer coatings simultaneously decrease the binding and uptake of AIE NPs by tumor cells.To overcome this problem,surface-adaptive AIE dye-encapsulated mixed-shell micelles(MSMs)with polyethylene glycol/poly(β-amino ester)(PEG/PAE)surfaces were prepared.Due to the charge conversion ability of PAE,MSMs demonstrated enhanced cellular uptake by tumor cells in acidic conditions.In addition,compared with single-PEG-shelled micelles(PEGSMs),MSMs exhibited prolonged systemic circulation due to the presence of micro-phase separated surfaces.Moreover,due to the coordination effect of enhanced cancer cell uptake and prolonged systemic circulation time,MSMs were more enriched than PEGSMs in the tumor cells and exhibited excellent performance during image-guided tumor resection.展开更多
基金supported by the National Natural Science Foundation of China (51622305, 51873092, 31771031, and 81701829)the National Basic Research Program of China (2015CB856503)+1 种基金the National Key Research and Development Program of China (2018YFA0209800)the Fundamental Research Funds for the Central Universities, Nankai University (63191521, 63171218 and 63191176)
文摘In recent years,organic/polymer photothermal nanoagents including semiconducting polymer nanoparticles and small-molecule organic photothermal agents-encapsulated nanoparticles have attracted large attention from researchers in the biomedical field,owing to their excellent optical properties,good biocompatibility,easy processability,and flexible surface functionalization,as well as their combined functions of photoacoustic(PA)imaging and photothermal therapy(PTT).In this review,we summarize the recent advances in organic/polymer photothermal nanoagents for in vivo PA imaging and PTT applications.In particular,we focus on the design strategies,which are composed of traditional approaches and emerging mechanisms,especially based on'intramolecular motion-induced photothermy'strategy to regulate the photophysical properties of organic/polymer photothermal nanoagents for boosted in vivo PA imaging and PTT.
基金supported by the National Natural Science Foundation of China(21674126,21790362,51873092,51961160730,31900983)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB12010100)+2 种基金the Science and Technology Commission of Shanghai Municipality(19XD1424700,18JC1410600,21QA1411100)the Tianjin Science Found for Distinguished Young Scholars(19JCJQJC61200)China Postdoctoral Science Foundation Grant(2019M660983)。
文摘Semiconducting polymers(SPs)with intensive near-infrared(NIR)absorption and high photothermal conversion efficiencies have been employed as a new generation of photothermal agents(PTAs)for“all-in-one”theranostic nanoplatforms with integrated photoacoustic imaging(PAI)and photothermal therapy(PTT)functions.However,the lack of facile molecular design principles impedes the development of highly efficient NIR PTAs.Herein,a facile molecular design strategy based on largeπ-extended donor-acceptor(L-π-D-A)structure is reported for achieving SPs(SP1-SP3)with highly efficient in vitro and in vivo PAI and PTT capabilities.Through adjusting the conjugation length and planarity of the donor units,both SP3 and corresponding nanoparticle(SPN)SPN3 exhibit stronger D-A strength,intensive NIR absorption,enhanced absorption coefficient,and higher photothermal conversion efficiency(up to 61.8%).The excellent photothermal conversion efficiencies make SPN1-SPN3 produce efficient inhibition of tumor growth with excellent biocompatibility and prominent PAI performance with a high contrast manner in living mice at a low systemic injection mass.Our research highlights that the new L-π-D-A molecular design is an effective strategy to obtain highly efficient polymeric NIR PTAs for high desirable cancer phototheranostic nanoplatforms.
基金supported by the National Natural Science Foundation of China (51622305, 51873092)the National Basic Research Program of China (2015CB856503)the Fundamental Research Funds for the Central Universities, Nankai University (63191521, 63171218, 63191176)
文摘The research in the biomedical applications of aggregation? induced emission luminogens (AIEgens) has attracted increasing interest in recent years, as AIEgens have been widely accepted as promising materials for constructing new generation of phototheranostic agents [1-5]. Different from conventional organic dyes, the AIEgens often have threedimensional (3D) molecular structure and contain a number of intramolecular motion units [6-8]. In the aggregates, the 3D molecular structure results in significantly reduced intermolecular interaction such as π-π stacking as compared to planar structure.
基金supported by the National Key R&D Program of China(Intergovernmental Cooperation Project,no.2017YFE0132200)the NSFC(nos.5191160730,51873092,and 81921004)the Tianjin Science Fund for Distinguished Young Scholars(no.19JCQJC61200).
文摘The combination of immunogenic cell death(ICD)induction and immune checkpoint blockade has emerged as a major direction of cancer immunotherapy.Among currently available ICD inducers,sonosensitizers that produce reactive oxygen species(ROS)under an external trigger to evoke ICD of tumor cells have shown great promise.However,a highly efficient sonosensitizer-based ICD inducer with an aggregationinduced emission(AIE)characteristic has yet to be developed.Herein,a novel AIE sonosensitizer with a twisted molecular structure,very small energy gap between the singlet and triplet excited states(ΔE_(ST)),and efficient ROS generation ability,which can serve as an effective ICD inducer,is reported for sonodynamic processes in cancer immunotherapy.Furthermore,an AIE sonosensitizer-based nanosystem with surface modification of anti-PD-L1 peptide is constructed for boosting antitumor immunotherapy.In this system,AIE sonosensitizer-mediated sonodynamic therapy can successfully convert a hypoimmunogenic cold tumor to a hot one and further facilitate the multivalent blocking of programed death ligand(PD-L1)by anti-PD-L1 peptides.Such an advanced nanosystem could effectively initiate the activation of antitumoral immune reactions and modulation of an immunosuppressive microenvironment,contributing to systemic antitumor effects to further inhibit the growth of distant tumors.
基金National Natural Science Foundation of China(51873092,51961160730,and 51673150)the National Key R&D Program of China(Intergovernmental Cooperation Project,2017YFE0132200)+2 种基金Tianjin Science Fund for Distinguished Young Scholars(19JCJQJC61200)Key Public Relations Project funded by Tianjin Health and Family Planning Commission(16KG103)the Fundamental Research Funds for the Central Universities,Nankai University。
文摘Phototheranostics that concurrently and complementarily integrate real-time diagnosis and in situ therapeutic capabilities in one platform has become the advancing edge of precision medicine.Organic agents possess the merits of facile preparation,high purity,tunable photophysical property,good biocompatibility,and potential biodegradability,which have shown great promise for disease theranostics.This review summarizes the recent achievements of organic phototheranostic agents and applications,especially which rationally utilize energy dissipation pathways of Jablonski diagram to modulate the fluorescence emission,photoacoustic/photothermal production,and photodynamic processes.Of particular interest are the systems exhibiting huge differences in aggregate state as compared with the solution or single molecule form,during which the intramolecular motions play an important role in regulating the photophysical properties.The recent advances from such an aspect for biomedical applications including high-resolution imaging,activatable imaging and therapy,adaptive theranostics,image-guided surgery,immunotherapy,and afterglow imaging are discussed.A brief summary and perspective in this field are also presented.We hope this review will be helpful to the researchers interested in bioprobe design and theranostic applications,and inspire new insights into the linkage between aggregate science and biomedical field.
基金supported by the NSFC (51622305 and 51873092)the National Basic Research Program of China (2015CB856503)the Fundamental Research Funds for the Central Universities, Nankai University (63191521, 63171218, and 63191176)
文摘Aggregation-induced emission(AIE)nanoparticles(NPs)are widely used for image-guided tumor resection because of their high signal-to-noise ratios and long systemic circulation time.These NPs are derived by encapsulating small-molecule fluorescent dyes with AIE property inside the cores of NPs assembled by amphiphilic polymers.Although the systemic circulation of AIE NPs is prolonged,hydrophilic polymer coatings simultaneously decrease the binding and uptake of AIE NPs by tumor cells.To overcome this problem,surface-adaptive AIE dye-encapsulated mixed-shell micelles(MSMs)with polyethylene glycol/poly(β-amino ester)(PEG/PAE)surfaces were prepared.Due to the charge conversion ability of PAE,MSMs demonstrated enhanced cellular uptake by tumor cells in acidic conditions.In addition,compared with single-PEG-shelled micelles(PEGSMs),MSMs exhibited prolonged systemic circulation due to the presence of micro-phase separated surfaces.Moreover,due to the coordination effect of enhanced cancer cell uptake and prolonged systemic circulation time,MSMs were more enriched than PEGSMs in the tumor cells and exhibited excellent performance during image-guided tumor resection.
