Biomedical scaffold fabrication has seen advancements in mimicking the native extracellular matrix through intricate three-dimensional(3D)structures conducive to tissue regeneration.Coiled fibrous scaffolds have emerg...Biomedical scaffold fabrication has seen advancements in mimicking the native extracellular matrix through intricate three-dimensional(3D)structures conducive to tissue regeneration.Coiled fibrous scaffolds have emerged as promising substrates owing to their ability to provide unique topographical cues.In this study,coiled poly(ε-caprolactone)(PCL)fibrous bundles were fabricated using an alginate-based composite system,and processed with 3D printing.The unique structure was obtained through the die-swell phenomenon related to the release of residual stresses from the printed strut,thereby transforming aligned PCL fibers into coiled structures.The effects of printing parameters,such as pneumatic pressure and nozzle moving speed,on fiber morphology were investigated to ensure a consistent formation of coiled PCL fibers.The resulting coiled PCL fibrous scaffold demonstrated higher activation of mechanotransduction signaling as well as upregulation of osteogenic-related genes in human adipose stem cells(hASCs),supporting its potential in bone tissue engineering.展开更多
Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss(VML).However,achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tiss...Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss(VML).However,achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tissue regeneration remains challenging,owing to difficulties in controlling cell arrangement within a low-viscosity hydrogel.Herein,we propose the concept of a magnetorheological bioink to manipulate the cellular arrangement within a low-viscosity hydrogel.This bioink consisted of gelatin methacrylate(GelMA),iron oxide nanoparticles,and human adipose stem cells(hASCs).The cell arrangement is regulated by the responsiveness of iron oxide nanoparticles to external magnetic fields.A bioprinting process using ring magnets was developed for in situ bioprinting,resulting in well-aligned 3D cell structures and enhanced mechanotransduction effects on hASCs.In vitro analyses revealed upregulation of cellular activities,including myogenic-related gene expression,in hASCs.When implanted into a VML mouse model,the bioconstructs improved muscle functionality and regeneration,validating the effectiveness of the proposed approach.展开更多
Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evi...Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.展开更多
基金supported by the‘Korea National Institute of Health’research project(2022ER130502)a grant from by SMC-SKKU Future Convergence Academic Research Program,2024supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(RS-2024-00336758)。
文摘Biomedical scaffold fabrication has seen advancements in mimicking the native extracellular matrix through intricate three-dimensional(3D)structures conducive to tissue regeneration.Coiled fibrous scaffolds have emerged as promising substrates owing to their ability to provide unique topographical cues.In this study,coiled poly(ε-caprolactone)(PCL)fibrous bundles were fabricated using an alginate-based composite system,and processed with 3D printing.The unique structure was obtained through the die-swell phenomenon related to the release of residual stresses from the printed strut,thereby transforming aligned PCL fibers into coiled structures.The effects of printing parameters,such as pneumatic pressure and nozzle moving speed,on fiber morphology were investigated to ensure a consistent formation of coiled PCL fibers.The resulting coiled PCL fibrous scaffold demonstrated higher activation of mechanotransduction signaling as well as upregulation of osteogenic-related genes in human adipose stem cells(hASCs),supporting its potential in bone tissue engineering.
基金supported by the’Korea National Institute of Health’research project(2022ER130502)a grant from by SMC-SKKU Future Convergence Academic Research Program,2024.In additionsupported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(RS-2024-00336758).
文摘Tissue-engineered anisotropic cell constructs are promising candidates for treating volumetric muscle loss(VML).However,achieving successful cell alignment within macroscale 3D cell constructs for skeletal muscle tissue regeneration remains challenging,owing to difficulties in controlling cell arrangement within a low-viscosity hydrogel.Herein,we propose the concept of a magnetorheological bioink to manipulate the cellular arrangement within a low-viscosity hydrogel.This bioink consisted of gelatin methacrylate(GelMA),iron oxide nanoparticles,and human adipose stem cells(hASCs).The cell arrangement is regulated by the responsiveness of iron oxide nanoparticles to external magnetic fields.A bioprinting process using ring magnets was developed for in situ bioprinting,resulting in well-aligned 3D cell structures and enhanced mechanotransduction effects on hASCs.In vitro analyses revealed upregulation of cellular activities,including myogenic-related gene expression,in hASCs.When implanted into a VML mouse model,the bioconstructs improved muscle functionality and regeneration,validating the effectiveness of the proposed approach.
基金supported by a grant from the National Research Foundation of Korea funded by the Ministry of education,Science,and Technology(MEST)(Grant NRF-2018R1A2B2005263)supported by the National Research Foundation of Korea(NRF)Grant funded by the Ministry of Science and ICT for Bioinspired Innovation Technology Development Project(NRF-2018M3C1B7021997)supported by a grant from the Ministry of Trade,Industry&Energy(MOTIE,Korea)under Industrial Technology Innovation Program(20009652:Technology on commercialization and materials of Bioabsorbable Hydroxyapatite that is less than micrometer in size).
文摘Volumetric muscle loss(VML)is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles.Tissue engineering has shown promise for the treatment of VML injuries,as evidenced by various preclinical trials.The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking(ISC)strategy to improve muscle functionality.To obtain optimal biophysical properties of the muscle construct,two UV exposure sources,UV exposure dose,and wall shear stress were evaluated using C2C12 myoblasts.Additionally,the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy(post-crosslinking).To evaluate the in vivo regenerative potential,muscle constructs laden with human adipose stem cells were used.The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume.The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.
