Defatted hickory meal(DHM),a by-product of hickory oil production,is a protein source rich in essential amino acids.In this study,the functional properties of DHM hydrolysate(DHMH)were assessed using in vitro and in v...Defatted hickory meal(DHM),a by-product of hickory oil production,is a protein source rich in essential amino acids.In this study,the functional properties of DHM hydrolysate(DHMH)were assessed using in vitro and in vivo assays in context to its antioxidant and memory-enhancing effects.To induce memory impairment,D-galactose(D-gal)was administered to mice at a dose of 120 mg/kg body weight per day,and DHMH was orally administered at doses of 300,600,and 1000 mg/kg body weight per day for 8 weeks.DHMH treatment led to improved memory performance in D-ga-induced memory-impaired mice,as observed in the Morris water maze test.Furthermore,DHMH mitigated the accumulation of amyloidβ_(1-42)triggered by D-gal exposure.Notably,high-dose DHMH significantly reduced the elevation of pro-inflammatory markers,including tumor necrosis factor alpha,interleukin 1β,and interleukin 6.Additionally,DHMH prevented the decline in total superoxide dismutase activity,glutathione peroxidase activity,and glutathione levels,while reducing malondialdehyde content in D-gal-induced mice,indicative of its antioxidant properties.Moreover,DHMH treatment effectively prevented histological alterations in neurons within the hippocampal CA1 area induced by D-gal.Collectively,our findings suggest that DHMH may counteract memory dysfunctions resulting from oxidative stress injury in the brain positioning it as a potential candidate for use as a functional food.展开更多
Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly compose...Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly composed of very long-chain fatty acids(VLCFAs);their derivatives,including esters,primary alcohols,secondary alcohols,aldehydes,and ketones;and triterpenoids.This complex mixture of lipids is probably biosynthesized in the epidermal cells of most plants and exuded onto the surface.Cuticular wax not only makes the fruit less susceptible to microbial infection but also reduces mechanical damage to the fruit,thereby maintaining the fruit’s commodity value.To date,research has mostly focused on the changes,function,and regulation of fruit wax before harvest,while ignoring the changes and functions of wax in fruit storage.This paper reviews on the composition,structure,and metabolic regulation of cuticular wax in fruits.It also focuses on postharvest factors affecting wax composition,such as storage temperature,relative humidity(RH),gas atmosphere,and as exogenous hormones;and the effects of wax on fruit postharvest quality,including water dispersion,fruit softening,physiological disorders,and disease resistance.These summaries may be of assistance in better understanding the changes in cuticular wax in postharvest fruit and the resulting effects on fruit quality.展开更多
基金supported by the Key Research and Development Project of Hanzhou(20231203A03)。
文摘Defatted hickory meal(DHM),a by-product of hickory oil production,is a protein source rich in essential amino acids.In this study,the functional properties of DHM hydrolysate(DHMH)were assessed using in vitro and in vivo assays in context to its antioxidant and memory-enhancing effects.To induce memory impairment,D-galactose(D-gal)was administered to mice at a dose of 120 mg/kg body weight per day,and DHMH was orally administered at doses of 300,600,and 1000 mg/kg body weight per day for 8 weeks.DHMH treatment led to improved memory performance in D-ga-induced memory-impaired mice,as observed in the Morris water maze test.Furthermore,DHMH mitigated the accumulation of amyloidβ_(1-42)triggered by D-gal exposure.Notably,high-dose DHMH significantly reduced the elevation of pro-inflammatory markers,including tumor necrosis factor alpha,interleukin 1β,and interleukin 6.Additionally,DHMH prevented the decline in total superoxide dismutase activity,glutathione peroxidase activity,and glutathione levels,while reducing malondialdehyde content in D-gal-induced mice,indicative of its antioxidant properties.Moreover,DHMH treatment effectively prevented histological alterations in neurons within the hippocampal CA1 area induced by D-gal.Collectively,our findings suggest that DHMH may counteract memory dysfunctions resulting from oxidative stress injury in the brain positioning it as a potential candidate for use as a functional food.
基金This work was supported by the National Natural Science Foundation of China(31772042)Ramóny Cajal grant(RYC2020-030365-I)+1 种基金Xunta de Galicia for supporting the program(Excelencia-ED431F2022/01)the Key Research&Development Program of Zhejiang Province(2021C02015).
文摘Cuticular wax plays a major role in the growth and storage of plant fruits.The cuticular wax coating,which covers the outermost layer of a fruit’s epidermal cells,is insoluble in water.Cuticular wax is mainly composed of very long-chain fatty acids(VLCFAs);their derivatives,including esters,primary alcohols,secondary alcohols,aldehydes,and ketones;and triterpenoids.This complex mixture of lipids is probably biosynthesized in the epidermal cells of most plants and exuded onto the surface.Cuticular wax not only makes the fruit less susceptible to microbial infection but also reduces mechanical damage to the fruit,thereby maintaining the fruit’s commodity value.To date,research has mostly focused on the changes,function,and regulation of fruit wax before harvest,while ignoring the changes and functions of wax in fruit storage.This paper reviews on the composition,structure,and metabolic regulation of cuticular wax in fruits.It also focuses on postharvest factors affecting wax composition,such as storage temperature,relative humidity(RH),gas atmosphere,and as exogenous hormones;and the effects of wax on fruit postharvest quality,including water dispersion,fruit softening,physiological disorders,and disease resistance.These summaries may be of assistance in better understanding the changes in cuticular wax in postharvest fruit and the resulting effects on fruit quality.
