Grape white rot caused by Coniella vitis is a global concern in the grape industry.pH regulation is essential for cell growth,reproductive processes and pathogenicity in phytopathogenic fungi.In this study,we observed...Grape white rot caused by Coniella vitis is a global concern in the grape industry.pH regulation is essential for cell growth,reproductive processes and pathogenicity in phytopathogenic fungi.In this study,we observed that the growth rate,spore production and virulence of C.vitis significantly declined in alkaline pH,as well as the suppressive effect on secretion of hydrolytic enzymes.Transcriptomic and metabolomic analyses were used to investigate the responses of C.vitis to acidic(pH 5),neutral(pH 7)and alkaline environments(pH 9).We identified 728,1,780 and 3,386 differentially expressed genes(DEGs)at pH 5,pH 7 and pH 9,when compared with the host pH(pH 3),and 2,122 differently expressed metabolites(DEMs)in negative and positive ion mode.Most DEGs were involved in carbohydrate metabolic process,transmembrane transport,tricarboxylic acid cycle,peptide metabolic process,amide biosynthetic process,and organic acid metabolic process.In addition,metabolomic analysis revealed ABC transporters,indole alkaloid biosynthesis,diterpenoid biosynthesis,and carotenoid biosynthesis pathways in response to the pH change.Furthermore,we found that the aspartate synthesis metabolic route associated with the TCA cycle is a key limiting factor for the growth and development of C.vitis in alkaline environments,and aspartate supplementation enables C.vitis to grow in alkaline environments.Plant cell wall-degrading enzymes(PCWDEs)could contribute to the pathogenicity,when C.vitis infected at pH 3.Importantly,aflatrem biosynthesis in acidic environment might contribute to the virulence of C.vitis and has a risk of causing human health problems due to its acute neurotoxic effects.展开更多
In this study,a pair of dicarboxylic acids as cis-trans isomerism—citraconic acid(CA)and mesaconic acid(MA),was incorporated into polymeric networks of poly(N-isopropylacrylamide)(PNIPAM)-based core-shell microgels v...In this study,a pair of dicarboxylic acids as cis-trans isomerism—citraconic acid(CA)and mesaconic acid(MA),was incorporated into polymeric networks of poly(N-isopropylacrylamide)(PNIPAM)-based core-shell microgels via semi-batch precipitation polymerization.We demonstrated that the pH-temperature dual responsiveness of the core-shell microgels is highly correlated with the structure and position of the acid isomers.Both the cis-trans molecular structure and the crosslinking position of the dicarboxylic acids significantly influenced the hydration capacity and surface charge density of the core-shell microgels.These diverse properties first influenced the swelling behavior,further affecting the interfacial behavior of the microgels,including the oil-water dynamic interfacial tension and air-water compression isotherms.Furthermore,the rheological behavior of the microgel suspensions also displayed distinct dependences on the frequency and temperature,illustrating that the cis-trans molecular structure and crosslinked position of the dicarboxylic acids also significantly influenced the interparticle clustering in the bulk solution.Our results suggest that the pH sensitivity of the cis-trans dicarboxylic acid isomer affects the ionization and surface charge distribution of the core or shell layers of individual microgels,which further determines the interparticle interaction and cooperative rearrangement at interfaces and in the bulk.展开更多
The Rpd3 histone deacetylase complex is a multiple-subunit complex that mediates the regulation of chromatin accessibility and gene expression.Sin3,the largest subunit of Rpd3 complex,is conserved in a broad range of ...The Rpd3 histone deacetylase complex is a multiple-subunit complex that mediates the regulation of chromatin accessibility and gene expression.Sin3,the largest subunit of Rpd3 complex,is conserved in a broad range of eukaryotes.Despite being a molecular scaffold for complex assembly,the functional sites and mechanism of action of Sin3 remain unexplored.In this study,we functionally characterized a glutamate residue(E810)in Fg Sin3,the ortholog of yeast Sin3 in Fusarium graminearum(known as wheat scab fungus).Our findings indicate that E810 was important for the functions of Fg Sin3 in regulating vegetative growth,sexual reproduction,wheat infection,and DON biosynthesis.Furthermore,the E810K missense mutation restored the reduced H4 acetylation caused by the deletion of FNG1,the ortholog of the human inhibitor of growth(ING1)gene in F.graminearum.Correspondingly,the defects of the fng1 mutant were also partially rescued by the E810K mutation in Fg Sin3.Sequence alignment and evolutionary analysis revealed that E810 residue is well-conserved in fungi,animals,and plants.Based on Alphafold2 structure modeling,E810 localized on the Fg Rpd3–Fg Sin3 interface for the formation of a hydrogen bond with Fg Rpd3.Mutation of E810 disrupts the hydrogen bond and likely affects the Fg Rpd3–Fg Sin3 interaction.Taken together,E810 of Fg Sin3 is functionally associated with Fng1 in the regulation of H4 acetylation and related biological processes,probably by affecting the assembly of the Rpd3 complex.展开更多
The Western Qinling Orogen(WQO)is characterized by voluminous distribution of Indosinian granitoids,the formation of which provides an important window to unravel the geochemical and geodynamic evolution and associate...The Western Qinling Orogen(WQO)is characterized by voluminous distribution of Indosinian granitoids,the formation of which provides an important window to unravel the geochemical and geodynamic evolution and associated metallogeny.Here we investigate a group of intrusions termed"Five Golden Flowers"based on petrological,geochemical,zircon U-Pb geochronological and Lu-Hf isotopic studies on the granitoids and their mafic microgranular enclaves(MMEs).Our results show that these intrusions are genetically divided into two types,namely,magma-mixing and highly fractionated.The Jiaochangba,Lujing,Zhongchuan,and Luchuba granitoids are biotite monzogranites(220±0.8 Ma to 217±2.6 Ma)with abundant coeval MMEs(220±.1 Ma to 217±2.7 Ma).The rocks contain moderate to high SiO2,high MgO,Rb,Sr,Ba,and Th contents,but low TiO2,P2 O5,and Sc values,A/CNK of<1.1,and a range ofεHf(t)values of-11.7 to+2.23 with corresponding TDM2values of 1967-1228 Ma.The MMEs possess K-feldspar megacrysts,abundant acicular apatites,and show lopsided textures.They have lower SiO2,Al2 O3,and Th contents,but higher MgO,TiO2,and Sc,withεHf(t)values of-18.0 to+3.18 and TDM1 of 849-720 Ma.The data indicate that the MMEs were derived from a magma sourced from the enriched lithospheric mantle.We suggest that these host granitoids were produced by partial melting of latePaleoproterozoic to early-Mesoproterozoic lower crust with the involvement of Neoproterozoic SCLM-derived mafic magmas.The Baijiazhuang pluton is dominantly composed of leucogranite(muscovite granite and twomica monzogranite,216±1.5 Ma)without MMEs.The rocks are peraluminous with high A/CNK(1.06-1.27).Compared with the other four granitoids,the Baijiazhuang leucogranite shows higher SiO2 content,markedly lower concentrations of TiO2,MgO,Al2 O3,CaO,and Fe2 O3T,and lower LREE/HREE and(La/Yb)N values.These leucogranites are also rich in Rb,Th,and U,and display marked depletions in Ba,Sr,Ti,and Eu,indicating that they experienced significant fractionation.ZirconεHf(t)values(-10.2 to-3.27)and TDM2(1868-1424 Ma),as well as the Nb/Ta and K2 O/Na2 O values are similar to the other four granitoids,indicating that they are likely to have been derived from a similar source;with sediments playing only a minor role in the magma generation.The low contents of Yb and Y suggest that their partial melting was controlled by garnets and micrographic texture of K-feldspar reflects high-temperature melting through undercooling.Based on the above features,we infer that the Baijiazhuang leucogranite likely represents the product of high degree fractionation of the I-type biotite monzogranite magma which generated the other four granitoids at relatively high temperatures,within magma chambers at mid-crust depths.We propose that the granitoid suite was formed in the transitional setting from synto post-collision during the collisional orogeny between the SCB and NCB,following break-off of the subducted South China Block lithosphere during 220-216 Ma.展开更多
In recent years,autonomous driving technology has made good progress,but the noncooperative intelligence of vehicle for autonomous driving still has many technical bottlenecks when facing urban road autonomous driving...In recent years,autonomous driving technology has made good progress,but the noncooperative intelligence of vehicle for autonomous driving still has many technical bottlenecks when facing urban road autonomous driving challenges.V2I(Vehicle-to-Infrastructure)communication is a potential solution to enable cooperative intelligence of vehicles and roads.In this paper,the RGB-PVRCNN,an environment perception framework,is proposed to improve the environmental awareness of autonomous vehicles at intersections by leveraging V2I communication technology.This framework integrates vision feature based on PVRCNN.The normal distributions transform(NDT)point cloud registration algorithm is deployed both on onboard and roadside to obtain the position of the autonomous vehicles and to build the local map objects detected by roadside multi-sensor system are sent back to autonomous vehicles to enhance the perception ability of autonomous vehicles for benefiting path planning and traffic efficiency at the intersection.The field-testing results show that our method can effectively extend the environmental perception ability and range of autonomous vehicles at the intersection and outperform the PointPillar algorithm and the VoxelRCNN algorithm in detection accuracy.展开更多
As an emerging joint learning model,federated learning is a promising way to combine model parameters of different users for training and inference without collecting users’original data.However,a practical and effic...As an emerging joint learning model,federated learning is a promising way to combine model parameters of different users for training and inference without collecting users’original data.However,a practical and efficient solution has not been established in previous work due to the absence of efficient matrix computation and cryptography schemes in the privacy-preserving federated learning model,especially in partially homomorphic cryptosystems.In this paper,we propose a Practical and Efficient Privacy-preserving Federated Learning(PEPFL)framework.First,we present a lifted distributed ElGamal cryptosystem for federated learning,which can solve the multi-key problem in federated learning.Secondly,we develop a Practical Partially Single Instruction Multiple Data(PSIMD)parallelism scheme that can encode a plaintext matrix into single plaintext for encryption,improving the encryption efficiency and reducing the communication cost in partially homomorphic cryptosystem.In addition,based on the Convolutional Neural Network(CNN)and the designed cryptosystem,a novel privacy-preserving federated learning framework is designed by using Momentum Gradient Descent(MGD).Finally,we evaluate the security and performance of PEPFL.The experiment results demonstrate that the scheme is practicable,effective,and secure with low communication and computation costs.展开更多
Transmission efficiency(TE) and focal ratio degradation(FRD) are two important parameters for evaluating the quality of an optical fiber system used for astronomy. Compared to TE, the focal ratio is more easily influe...Transmission efficiency(TE) and focal ratio degradation(FRD) are two important parameters for evaluating the quality of an optical fiber system used for astronomy. Compared to TE, the focal ratio is more easily influenced by external factors, such as bending or stress. Optical cables are widely implemented for multi-object telescopes and integral field units(IFUs). The design and fabrication process of traditional optical cables seldom considers the requirements of astronomical applications. In this paper, we describe a fiber bundle structure as the basic unit for miniaturized high-density FASOT-IFU optical cables,instead of the micro-tube structure in stranded cables. Seven fibers with hexagonal arrangement were accurately positioned by ultraviolet(UV)-curing acrylate to form the bundle. The coating diameter of a fiber is0.125 mm, and the outer diameter of the bundle is 0.58 mm. Compared with the 0.8 mm micro-tube structure of a traditional stranded cable, the outer diameter of the fiber bundle was reduced by 27.5%. Fiber paste was filled into the bundle to reduce stress between the fibers. We tested the output focal ratio(OFR) in95% of the encircled energy(EE95) of the fibers in the bundle under different conditions. With the incident focal ratio F/8, the maximum difference of OFR is 0.6. In particular, when the incident focal ratio is F/5,the maximum difference of OFR is only 0.1. The jacket formed by the UV-curing acrylate can withstand a certain stress of less than 1.38 N mm-1. The fiber bundle can maintain uniform emitting characteristics with a bending radius of 7.5 cm and with tension less than 6 N. The test results show that the structure of the fiber bundle can be used as a basic unit for miniaturized high-density astronomical optical cables.展开更多
To improve the estimation accuracy,a novel time delay estimation(TDE)method based on the closed-form offset compensation is proposed.Firstly,we use the generalized cross-correlation with phase transform(GCC-PHAT)metho...To improve the estimation accuracy,a novel time delay estimation(TDE)method based on the closed-form offset compensation is proposed.Firstly,we use the generalized cross-correlation with phase transform(GCC-PHAT)method to obtain the initial TDE.Secondly,a signal model using normalized cross spectrum is established,and the noise subspace is extracted by eigenvalue decomposition(EVD)of covariance matrix.Using the orthogonal relation between the steering vector and the noise subspace,the first-order Taylor expansion is carried out on the steering vector reconstructed by the initial TDE.Finally,the offsets are compensated via simple least squares(LS).Compared to other state-of-the-art methods,the proposed method significantly reduces the computational complexity and achieves better estimation performance.Experiments on both simulation and real-world data verify the efficiency of the proposed approach.展开更多
Microbial cultivation in microcompartments using water-in-oil(w/o)emulsion droplets has greatly advanced the study of strict anaerobic bacteria.However,a major challenge lies in the separation and recovery of the bact...Microbial cultivation in microcompartments using water-in-oil(w/o)emulsion droplets has greatly advanced the study of strict anaerobic bacteria.However,a major challenge lies in the separation and recovery of the bacterial liquid.In this study,we address this challenge by localizing strict anaerobes within w/o Pickering emulsion droplets stabilized by modified microgel particles.This approach demonstrates remarkable efficiency in bacterial microencapsulation,growth and separation.The microgel particles are modified in-situ with hydrophobic silica allowing for stabilization of w/o emulsions at the optimum cultivation temperature of 37℃ while the continuous oil phase prevents oxygen ingress thereby maximizing anaerobic fermentation.Additionally,a temperature decrease triggers the microgel particles to swell and become hydrophilic,causing an immediate inversion of the emulsion to oil-in-water type.This facilitates complete separation and collection of the bacterial suspension without any damage or loss.The intelligent phase inversion of the emulsion presents exciting possibilities for various applications in anaerobic fermentation,synthetic biology and biomanufacturing.展开更多
The establishment of the China Pilot Free Trade Zone(FTZ) has significantly promoted international trade, financial development, and economic growth. Building international financial centers(IFCs) satisfies the demand...The establishment of the China Pilot Free Trade Zone(FTZ) has significantly promoted international trade, financial development, and economic growth. Building international financial centers(IFCs) satisfies the demand for FTZs to facilitate financial development, as well as promoting economic growth. Thus, successfully predicting the next IFC in China under the FTZ framework is an important issue. In this study, we applied grey relational analysis combined with entropy method to predict potential IFCs among seven FTZ cities. According to the results, our interesting findings include: 1) the "total stock turnover", "total value of imports and exports", and "Foreign Direct Investment(FDI)" are key indicators for determining future IFCs; 2) among seven cities, Shenzhen and Tianjin are highly likely to become the next IFCs, while Shanghai is already an IFC.展开更多
Purpose–With the development of economy,China’s OFDI constantly increase in recent year.Meanwhile,OFDI hasspillovereffectoneconomicdevelopmentandtechnologicaldevelopmentofhomecountry.Thus,accurateOFDI prediction is ...Purpose–With the development of economy,China’s OFDI constantly increase in recent year.Meanwhile,OFDI hasspillovereffectoneconomicdevelopmentandtechnologicaldevelopmentofhomecountry.Thus,accurateOFDI prediction is a prerequisite for the effective development of international investment strategies.The purpose of this paper is to predict China’s OFDI accurately using a novel multivariable grey prediction model with Fourier series.Design/methodology/approach–This paper applied a multivariable grey prediction model,GM(1,N),to forecast China’s OFDI.In order to improve the prediction accuracy and without changing local characteristics of grey model prediction,this paper proposed a novel grey prediction model to improve the performance of the traditionalGM(1,N)modelbycombiningwithresidualmodificationmodelusingGM(1,1)modelandFourierseries.Findings–The coefficients indicate that the export and GDP have positive influence on China’s OFDI,and,according to the prediction result,China’s OFDI shows a growing trend in next five years.Originality/value–This paper proposed an effective multivariable grey prediction model that combined the traditionalGM(1,N)modelwitharesidualmodificationmodelinordertopredictChina’sOFDI.Accurateforecasting of OFDI provides reference for the Chinese Government to implement international investment strategies.展开更多
Background:Cholangiocarcinoma(CCA)is a diverse group of malignancies arising from the intra-or extrahepatic biliary epithelium and characterized by its late diagnosis and fatal outcome.Extrahepatic cholangiocarcinoma(...Background:Cholangiocarcinoma(CCA)is a diverse group of malignancies arising from the intra-or extrahepatic biliary epithelium and characterized by its late diagnosis and fatal outcome.Extrahepatic cholangiocarcinoma(ECC)accounts for 90%of CCA.However,little is known about the comprehensive genomic alterations of ECC in Chinese population for providing clinical managements especially targeted therapy.Methods:Comprehensive genomic profiling(CGP)was performed with next generation sequencing panel on paraffin-embedded tumor from a cohort of 80 Chinese ECC patients.Results:The most frequently altered genes were TP53(68%),KRAS(46%),SMAD4(22%),ARID1A(20%)and CDKN2A(19%).Mutual exclusivity was observed between multiple genes including ARID1A:TP53,KRAS:LRP1B and NF2:TP53.Genetic alterations with potential therapeutic implications were identified in 43%of patients.The top three actionable alterations include CDKN2A(n=11),BRAF(n=5)and ERBB2(n=4).Potentially actionable alterations were mainly enriched in the G1-S transition,homologous recombination repair,MAPK/ERK pathway.Conclusions:This is the largest data set of ECC cases providing a comprehensive view on genetic alterations in Chinese population which differs significantly from a US cohort,and indicates the potential clinical implications for targeted therapies.展开更多
A torsion sensor based on the near-helical(NH)long period fiber grating(LPFG)is fabricated by using a high frequency pulsed CO_(2) laser.Each groove of the NH-LPFG is spirally written in the four sides of a single-mod...A torsion sensor based on the near-helical(NH)long period fiber grating(LPFG)is fabricated by using a high frequency pulsed CO_(2) laser.Each groove of the NH-LPFG is spirally written in the four sides of a single-mode fiber.The NH-LPFG has a helical periodic vertical index modulation.This is different from the screw-type index modulation of the common helical LPFGs(H-LPFGs)fabricated in a twisted fiber.The torsion and temperature characteristics of the NH-LPFG are experimentally investigated.The temperature sensitivity is about0.0668 nm/℃.The torsion sensitivity is 0.103 nm/(rad/m)and independent of the polarization state of incident light.展开更多
Plasma protein-induced aggregation of nanoparticles(NPs)is a crucial issue in many applications,such as drug delivery.Although great efforts have been made to inves-tigate the protein adsorption kinetics or protein-in...Plasma protein-induced aggregation of nanoparticles(NPs)is a crucial issue in many applications,such as drug delivery.Although great efforts have been made to inves-tigate the protein adsorption kinetics or protein-induced NPs coalescence in bulk solutions,limited evidence has been uncovered for interfacial circumstances.Diet,disease,medicine,or senility could thoroughly change interfacial physicochemical properties of the inner lining of blood vessels.Implants including stents and artificial heart valves also have varied and evolutionary interfaces.Hence,there is an urgent need to understand the mechanism behind the non-specific protein adsorption and NP-protein aggregation in such interfacial cases.Here,we use evanescent light scat-tering to observe polystyrene NPs‒fibrinogen aggregation at substrates with varying surface properties.A density-fluctuation correlation function is utilized to reveal the relaxation dynamics of the aggregates.Both time-resolved and spatial-correlated evi-dence shows that the aging process of such soft materials is out-of-equilibrium,where the dynamics faster and slower than exponential can coexist in one sin-gle relaxation process.Besides,corona formation,inner stress,and interconnection together determine the microstructure,local adhesion,and structural relaxation of the aggregates,which can further correspond to the protein-to-NP ratio as well as the surface chemistry of NPs and substrates.展开更多
基金supported by the Shandong Provincial Natural Science Foundation,China(ZR2021QC131)the Shandong Province Key Research and Development Plan,China(2022TZXD001102)+1 种基金the Shandong Province Demonstration Project for Model Construction in Rural Revitalization Service,China(2022DXAL0226)the Innovation Project of Shandong Academy of Agricultural Sciences,China(CXGC2023F15,CXGC2023A41,and CXGC2023A47)。
文摘Grape white rot caused by Coniella vitis is a global concern in the grape industry.pH regulation is essential for cell growth,reproductive processes and pathogenicity in phytopathogenic fungi.In this study,we observed that the growth rate,spore production and virulence of C.vitis significantly declined in alkaline pH,as well as the suppressive effect on secretion of hydrolytic enzymes.Transcriptomic and metabolomic analyses were used to investigate the responses of C.vitis to acidic(pH 5),neutral(pH 7)and alkaline environments(pH 9).We identified 728,1,780 and 3,386 differentially expressed genes(DEGs)at pH 5,pH 7 and pH 9,when compared with the host pH(pH 3),and 2,122 differently expressed metabolites(DEMs)in negative and positive ion mode.Most DEGs were involved in carbohydrate metabolic process,transmembrane transport,tricarboxylic acid cycle,peptide metabolic process,amide biosynthetic process,and organic acid metabolic process.In addition,metabolomic analysis revealed ABC transporters,indole alkaloid biosynthesis,diterpenoid biosynthesis,and carotenoid biosynthesis pathways in response to the pH change.Furthermore,we found that the aspartate synthesis metabolic route associated with the TCA cycle is a key limiting factor for the growth and development of C.vitis in alkaline environments,and aspartate supplementation enables C.vitis to grow in alkaline environments.Plant cell wall-degrading enzymes(PCWDEs)could contribute to the pathogenicity,when C.vitis infected at pH 3.Importantly,aflatrem biosynthesis in acidic environment might contribute to the virulence of C.vitis and has a risk of causing human health problems due to its acute neurotoxic effects.
基金financially supported by the National Natural Science Foundation of China(No.22303033)the Fundamental Research Funds for the Central Universities,China(No.JUSRP123017)+1 种基金Wuxi“Taihu Light”Science and Technology Project-Basic Research(No.K20231063)the Research Matching Grant Scheme at CUHK(No.8601309)。
文摘In this study,a pair of dicarboxylic acids as cis-trans isomerism—citraconic acid(CA)and mesaconic acid(MA),was incorporated into polymeric networks of poly(N-isopropylacrylamide)(PNIPAM)-based core-shell microgels via semi-batch precipitation polymerization.We demonstrated that the pH-temperature dual responsiveness of the core-shell microgels is highly correlated with the structure and position of the acid isomers.Both the cis-trans molecular structure and the crosslinking position of the dicarboxylic acids significantly influenced the hydration capacity and surface charge density of the core-shell microgels.These diverse properties first influenced the swelling behavior,further affecting the interfacial behavior of the microgels,including the oil-water dynamic interfacial tension and air-water compression isotherms.Furthermore,the rheological behavior of the microgel suspensions also displayed distinct dependences on the frequency and temperature,illustrating that the cis-trans molecular structure and crosslinked position of the dicarboxylic acids also significantly influenced the interparticle clustering in the bulk solution.Our results suggest that the pH sensitivity of the cis-trans dicarboxylic acid isomer affects the ionization and surface charge distribution of the core or shell layers of individual microgels,which further determines the interparticle interaction and cooperative rearrangement at interfaces and in the bulk.
基金supported by the grants from the National Natural Science Foundation of China(32102181)the Shaanxi Science Fund for Distinguished Young Scholars,China(2022JC-14)。
文摘The Rpd3 histone deacetylase complex is a multiple-subunit complex that mediates the regulation of chromatin accessibility and gene expression.Sin3,the largest subunit of Rpd3 complex,is conserved in a broad range of eukaryotes.Despite being a molecular scaffold for complex assembly,the functional sites and mechanism of action of Sin3 remain unexplored.In this study,we functionally characterized a glutamate residue(E810)in Fg Sin3,the ortholog of yeast Sin3 in Fusarium graminearum(known as wheat scab fungus).Our findings indicate that E810 was important for the functions of Fg Sin3 in regulating vegetative growth,sexual reproduction,wheat infection,and DON biosynthesis.Furthermore,the E810K missense mutation restored the reduced H4 acetylation caused by the deletion of FNG1,the ortholog of the human inhibitor of growth(ING1)gene in F.graminearum.Correspondingly,the defects of the fng1 mutant were also partially rescued by the E810K mutation in Fg Sin3.Sequence alignment and evolutionary analysis revealed that E810 residue is well-conserved in fungi,animals,and plants.Based on Alphafold2 structure modeling,E810 localized on the Fg Rpd3–Fg Sin3 interface for the formation of a hydrogen bond with Fg Rpd3.Mutation of E810 disrupts the hydrogen bond and likely affects the Fg Rpd3–Fg Sin3 interaction.Taken together,E810 of Fg Sin3 is functionally associated with Fng1 in the regulation of H4 acetylation and related biological processes,probably by affecting the assembly of the Rpd3 complex.
基金jointly supported by National Natural Science Foundation of China(Grant Nos.41730426,41421002,41272092 and 41803039)MOST Special Fund from the State Key Laboratory of Continental Dynamics,Northwest University,China
文摘The Western Qinling Orogen(WQO)is characterized by voluminous distribution of Indosinian granitoids,the formation of which provides an important window to unravel the geochemical and geodynamic evolution and associated metallogeny.Here we investigate a group of intrusions termed"Five Golden Flowers"based on petrological,geochemical,zircon U-Pb geochronological and Lu-Hf isotopic studies on the granitoids and their mafic microgranular enclaves(MMEs).Our results show that these intrusions are genetically divided into two types,namely,magma-mixing and highly fractionated.The Jiaochangba,Lujing,Zhongchuan,and Luchuba granitoids are biotite monzogranites(220±0.8 Ma to 217±2.6 Ma)with abundant coeval MMEs(220±.1 Ma to 217±2.7 Ma).The rocks contain moderate to high SiO2,high MgO,Rb,Sr,Ba,and Th contents,but low TiO2,P2 O5,and Sc values,A/CNK of<1.1,and a range ofεHf(t)values of-11.7 to+2.23 with corresponding TDM2values of 1967-1228 Ma.The MMEs possess K-feldspar megacrysts,abundant acicular apatites,and show lopsided textures.They have lower SiO2,Al2 O3,and Th contents,but higher MgO,TiO2,and Sc,withεHf(t)values of-18.0 to+3.18 and TDM1 of 849-720 Ma.The data indicate that the MMEs were derived from a magma sourced from the enriched lithospheric mantle.We suggest that these host granitoids were produced by partial melting of latePaleoproterozoic to early-Mesoproterozoic lower crust with the involvement of Neoproterozoic SCLM-derived mafic magmas.The Baijiazhuang pluton is dominantly composed of leucogranite(muscovite granite and twomica monzogranite,216±1.5 Ma)without MMEs.The rocks are peraluminous with high A/CNK(1.06-1.27).Compared with the other four granitoids,the Baijiazhuang leucogranite shows higher SiO2 content,markedly lower concentrations of TiO2,MgO,Al2 O3,CaO,and Fe2 O3T,and lower LREE/HREE and(La/Yb)N values.These leucogranites are also rich in Rb,Th,and U,and display marked depletions in Ba,Sr,Ti,and Eu,indicating that they experienced significant fractionation.ZirconεHf(t)values(-10.2 to-3.27)and TDM2(1868-1424 Ma),as well as the Nb/Ta and K2 O/Na2 O values are similar to the other four granitoids,indicating that they are likely to have been derived from a similar source;with sediments playing only a minor role in the magma generation.The low contents of Yb and Y suggest that their partial melting was controlled by garnets and micrographic texture of K-feldspar reflects high-temperature melting through undercooling.Based on the above features,we infer that the Baijiazhuang leucogranite likely represents the product of high degree fractionation of the I-type biotite monzogranite magma which generated the other four granitoids at relatively high temperatures,within magma chambers at mid-crust depths.We propose that the granitoid suite was formed in the transitional setting from synto post-collision during the collisional orogeny between the SCB and NCB,following break-off of the subducted South China Block lithosphere during 220-216 Ma.
基金This research was supported by the National Key Research and Development Program of China under Grant No.2017YFB0102502the Beijing Municipal Natural Science Foundation No.L191001+2 种基金the National Natural Science Foundation of China under Grant No.61672082 and 61822101the Newton Advanced Fellowship under Grant No.62061130221the Young Elite Scientists Sponsorship Program by Hunan Provincial Department of Education under Grant No.18B142.
文摘In recent years,autonomous driving technology has made good progress,but the noncooperative intelligence of vehicle for autonomous driving still has many technical bottlenecks when facing urban road autonomous driving challenges.V2I(Vehicle-to-Infrastructure)communication is a potential solution to enable cooperative intelligence of vehicles and roads.In this paper,the RGB-PVRCNN,an environment perception framework,is proposed to improve the environmental awareness of autonomous vehicles at intersections by leveraging V2I communication technology.This framework integrates vision feature based on PVRCNN.The normal distributions transform(NDT)point cloud registration algorithm is deployed both on onboard and roadside to obtain the position of the autonomous vehicles and to build the local map objects detected by roadside multi-sensor system are sent back to autonomous vehicles to enhance the perception ability of autonomous vehicles for benefiting path planning and traffic efficiency at the intersection.The field-testing results show that our method can effectively extend the environmental perception ability and range of autonomous vehicles at the intersection and outperform the PointPillar algorithm and the VoxelRCNN algorithm in detection accuracy.
基金supported by the National Natural Science Foundation of China under Grant No.U19B2021the Key Research and Development Program of Shaanxi under Grant No.2020ZDLGY08-04+1 种基金the Key Technologies R&D Program of He’nan Province under Grant No.212102210084the Innovation Scientists and Technicians Troop Construction Projects of Henan Province.
文摘As an emerging joint learning model,federated learning is a promising way to combine model parameters of different users for training and inference without collecting users’original data.However,a practical and efficient solution has not been established in previous work due to the absence of efficient matrix computation and cryptography schemes in the privacy-preserving federated learning model,especially in partially homomorphic cryptosystems.In this paper,we propose a Practical and Efficient Privacy-preserving Federated Learning(PEPFL)framework.First,we present a lifted distributed ElGamal cryptosystem for federated learning,which can solve the multi-key problem in federated learning.Secondly,we develop a Practical Partially Single Instruction Multiple Data(PSIMD)parallelism scheme that can encode a plaintext matrix into single plaintext for encryption,improving the encryption efficiency and reducing the communication cost in partially homomorphic cryptosystem.In addition,based on the Convolutional Neural Network(CNN)and the designed cryptosystem,a novel privacy-preserving federated learning framework is designed by using Momentum Gradient Descent(MGD).Finally,we evaluate the security and performance of PEPFL.The experiment results demonstrate that the scheme is practicable,effective,and secure with low communication and computation costs.
基金funded by the Joint Research Fund in Astronomy under cooperative agreement between the National Natural Science Foundation of China (NSFC)the Chinese Academy of Sciences (Grant Nos. U1631239 and U1831115)+1 种基金the NSFC (Grant No. 11603008)the Fundamental Research Funds for Central Universities to Harbin Engineering University
文摘Transmission efficiency(TE) and focal ratio degradation(FRD) are two important parameters for evaluating the quality of an optical fiber system used for astronomy. Compared to TE, the focal ratio is more easily influenced by external factors, such as bending or stress. Optical cables are widely implemented for multi-object telescopes and integral field units(IFUs). The design and fabrication process of traditional optical cables seldom considers the requirements of astronomical applications. In this paper, we describe a fiber bundle structure as the basic unit for miniaturized high-density FASOT-IFU optical cables,instead of the micro-tube structure in stranded cables. Seven fibers with hexagonal arrangement were accurately positioned by ultraviolet(UV)-curing acrylate to form the bundle. The coating diameter of a fiber is0.125 mm, and the outer diameter of the bundle is 0.58 mm. Compared with the 0.8 mm micro-tube structure of a traditional stranded cable, the outer diameter of the fiber bundle was reduced by 27.5%. Fiber paste was filled into the bundle to reduce stress between the fibers. We tested the output focal ratio(OFR) in95% of the encircled energy(EE95) of the fibers in the bundle under different conditions. With the incident focal ratio F/8, the maximum difference of OFR is 0.6. In particular, when the incident focal ratio is F/5,the maximum difference of OFR is only 0.1. The jacket formed by the UV-curing acrylate can withstand a certain stress of less than 1.38 N mm-1. The fiber bundle can maintain uniform emitting characteristics with a bending radius of 7.5 cm and with tension less than 6 N. The test results show that the structure of the fiber bundle can be used as a basic unit for miniaturized high-density astronomical optical cables.
基金supported in part by National Key R&D Program of China under Grants 2020YFB1807602 and 2020YFB1807600National Science Foundation of China(61971217,61971218,61631020,61601167)+1 种基金the Fund of Sonar Technology Key Laboratory(Range estimation and location technology of passive target viamultiple array combination),Jiangsu Planned Projects for Postdoctoral Research Funds(2020Z013)China Postdoctoral Science Foundation(2020M681585).
文摘To improve the estimation accuracy,a novel time delay estimation(TDE)method based on the closed-form offset compensation is proposed.Firstly,we use the generalized cross-correlation with phase transform(GCC-PHAT)method to obtain the initial TDE.Secondly,a signal model using normalized cross spectrum is established,and the noise subspace is extracted by eigenvalue decomposition(EVD)of covariance matrix.Using the orthogonal relation between the steering vector and the noise subspace,the first-order Taylor expansion is carried out on the steering vector reconstructed by the initial TDE.Finally,the offsets are compensated via simple least squares(LS).Compared to other state-of-the-art methods,the proposed method significantly reduces the computational complexity and achieves better estimation performance.Experiments on both simulation and real-world data verify the efficiency of the proposed approach.
基金financial support from the National Natural Science Foundation of China(grant no.22202084)Natural Science Foundation of Jiangsu Province(grant no.BK20221059)+1 种基金the Fundamental Research Funds for the Central Universities(grant no.JUSRP122017)the Research Matching Grant Scheme at CUHK(grant no.8601309).
文摘Microbial cultivation in microcompartments using water-in-oil(w/o)emulsion droplets has greatly advanced the study of strict anaerobic bacteria.However,a major challenge lies in the separation and recovery of the bacterial liquid.In this study,we address this challenge by localizing strict anaerobes within w/o Pickering emulsion droplets stabilized by modified microgel particles.This approach demonstrates remarkable efficiency in bacterial microencapsulation,growth and separation.The microgel particles are modified in-situ with hydrophobic silica allowing for stabilization of w/o emulsions at the optimum cultivation temperature of 37℃ while the continuous oil phase prevents oxygen ingress thereby maximizing anaerobic fermentation.Additionally,a temperature decrease triggers the microgel particles to swell and become hydrophilic,causing an immediate inversion of the emulsion to oil-in-water type.This facilitates complete separation and collection of the bacterial suspension without any damage or loss.The intelligent phase inversion of the emulsion presents exciting possibilities for various applications in anaerobic fermentation,synthetic biology and biomanufacturing.
文摘The establishment of the China Pilot Free Trade Zone(FTZ) has significantly promoted international trade, financial development, and economic growth. Building international financial centers(IFCs) satisfies the demand for FTZs to facilitate financial development, as well as promoting economic growth. Thus, successfully predicting the next IFC in China under the FTZ framework is an important issue. In this study, we applied grey relational analysis combined with entropy method to predict potential IFCs among seven FTZ cities. According to the results, our interesting findings include: 1) the "total stock turnover", "total value of imports and exports", and "Foreign Direct Investment(FDI)" are key indicators for determining future IFCs; 2) among seven cities, Shenzhen and Tianjin are highly likely to become the next IFCs, while Shanghai is already an IFC.
文摘Purpose–With the development of economy,China’s OFDI constantly increase in recent year.Meanwhile,OFDI hasspillovereffectoneconomicdevelopmentandtechnologicaldevelopmentofhomecountry.Thus,accurateOFDI prediction is a prerequisite for the effective development of international investment strategies.The purpose of this paper is to predict China’s OFDI accurately using a novel multivariable grey prediction model with Fourier series.Design/methodology/approach–This paper applied a multivariable grey prediction model,GM(1,N),to forecast China’s OFDI.In order to improve the prediction accuracy and without changing local characteristics of grey model prediction,this paper proposed a novel grey prediction model to improve the performance of the traditionalGM(1,N)modelbycombiningwithresidualmodificationmodelusingGM(1,1)modelandFourierseries.Findings–The coefficients indicate that the export and GDP have positive influence on China’s OFDI,and,according to the prediction result,China’s OFDI shows a growing trend in next five years.Originality/value–This paper proposed an effective multivariable grey prediction model that combined the traditionalGM(1,N)modelwitharesidualmodificationmodelinordertopredictChina’sOFDI.Accurateforecasting of OFDI provides reference for the Chinese Government to implement international investment strategies.
文摘Background:Cholangiocarcinoma(CCA)is a diverse group of malignancies arising from the intra-or extrahepatic biliary epithelium and characterized by its late diagnosis and fatal outcome.Extrahepatic cholangiocarcinoma(ECC)accounts for 90%of CCA.However,little is known about the comprehensive genomic alterations of ECC in Chinese population for providing clinical managements especially targeted therapy.Methods:Comprehensive genomic profiling(CGP)was performed with next generation sequencing panel on paraffin-embedded tumor from a cohort of 80 Chinese ECC patients.Results:The most frequently altered genes were TP53(68%),KRAS(46%),SMAD4(22%),ARID1A(20%)and CDKN2A(19%).Mutual exclusivity was observed between multiple genes including ARID1A:TP53,KRAS:LRP1B and NF2:TP53.Genetic alterations with potential therapeutic implications were identified in 43%of patients.The top three actionable alterations include CDKN2A(n=11),BRAF(n=5)and ERBB2(n=4).Potentially actionable alterations were mainly enriched in the G1-S transition,homologous recombination repair,MAPK/ERK pathway.Conclusions:This is the largest data set of ECC cases providing a comprehensive view on genetic alterations in Chinese population which differs significantly from a US cohort,and indicates the potential clinical implications for targeted therapies.
基金supported by the National Natural Science Foundation of China(Nos.61377084,41174161,and 61775044)the Joint Research Fund in Astronomy(No.U1631239)under cooperative agreement between the National Natural Science Foundation of China(NSFC)and the Chinese Academy of Sciences(CAS)in part by the Aeronautical Science Foundation of China(No.201608P6003)
文摘A torsion sensor based on the near-helical(NH)long period fiber grating(LPFG)is fabricated by using a high frequency pulsed CO_(2) laser.Each groove of the NH-LPFG is spirally written in the four sides of a single-mode fiber.The NH-LPFG has a helical periodic vertical index modulation.This is different from the screw-type index modulation of the common helical LPFGs(H-LPFGs)fabricated in a twisted fiber.The torsion and temperature characteristics of the NH-LPFG are experimentally investigated.The temperature sensitivity is about0.0668 nm/℃.The torsion sensitivity is 0.103 nm/(rad/m)and independent of the polarization state of incident light.
基金National Natural Science Foundation of China,Grant/Award Number:22303033Fundamental Research Funds for the Central Universities of China,Grant/Award Number:JUSRP123017+2 种基金Wuxi“Taihu Light”Science and Technology Project-Basic Research,Grant/Award Number:K20231063Hong Kong Metropolitan University,Grant/Award Number:RD/2023/2.1Hong Kong Special Administration Region(HKSAR)General Research Fund,Grant/Award Numbers:CUHK14302120,2130704。
文摘Plasma protein-induced aggregation of nanoparticles(NPs)is a crucial issue in many applications,such as drug delivery.Although great efforts have been made to inves-tigate the protein adsorption kinetics or protein-induced NPs coalescence in bulk solutions,limited evidence has been uncovered for interfacial circumstances.Diet,disease,medicine,or senility could thoroughly change interfacial physicochemical properties of the inner lining of blood vessels.Implants including stents and artificial heart valves also have varied and evolutionary interfaces.Hence,there is an urgent need to understand the mechanism behind the non-specific protein adsorption and NP-protein aggregation in such interfacial cases.Here,we use evanescent light scat-tering to observe polystyrene NPs‒fibrinogen aggregation at substrates with varying surface properties.A density-fluctuation correlation function is utilized to reveal the relaxation dynamics of the aggregates.Both time-resolved and spatial-correlated evi-dence shows that the aging process of such soft materials is out-of-equilibrium,where the dynamics faster and slower than exponential can coexist in one sin-gle relaxation process.Besides,corona formation,inner stress,and interconnection together determine the microstructure,local adhesion,and structural relaxation of the aggregates,which can further correspond to the protein-to-NP ratio as well as the surface chemistry of NPs and substrates.
