A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement.First,two micro-nano thermocouples(TC)and a high-pr...A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement.First,two micro-nano thermocouples(TC)and a high-precision signal acquisition module are used to measure the temperature difference between the cell and the culture medium(separated about 10μm from the cell).The cold junctions of two TCs are connected to eliminate the setting of the reference temperature and enhance the anti-interference ability.Then,a low-noise voltage amplifier and digital acquisition card are used to sample signals.In order to verify the feasibility of the dual thermocouple difference method,the temperature changes of U251 cells are detected.The calibration results of two TCs show that the Seebeck coefficient is about 5μV/℃,and the signal acquisition accuracy is 0.5μV in a low voltage range(0-15μV).With the dual thermocouple difference method,errors due to the cold junctions can be removed and the interference caused by environmental temperature fluctuation can be reduced.The phenomenon of cellular temperature increase proves that the dual thermocouple difference method can detect the tiny temperature change of a single cell.The method potentially is a highly powerful technique for studying local thermogenesis of cells and helps to explore the relationship between cellular thermogenesis and cellular processes.展开更多
基金The National Key Research and Development Program of China(No.2017YFA0104302)the National Natural Science Foundation of China(No.61420106012,61821002).
文摘A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement.First,two micro-nano thermocouples(TC)and a high-precision signal acquisition module are used to measure the temperature difference between the cell and the culture medium(separated about 10μm from the cell).The cold junctions of two TCs are connected to eliminate the setting of the reference temperature and enhance the anti-interference ability.Then,a low-noise voltage amplifier and digital acquisition card are used to sample signals.In order to verify the feasibility of the dual thermocouple difference method,the temperature changes of U251 cells are detected.The calibration results of two TCs show that the Seebeck coefficient is about 5μV/℃,and the signal acquisition accuracy is 0.5μV in a low voltage range(0-15μV).With the dual thermocouple difference method,errors due to the cold junctions can be removed and the interference caused by environmental temperature fluctuation can be reduced.The phenomenon of cellular temperature increase proves that the dual thermocouple difference method can detect the tiny temperature change of a single cell.The method potentially is a highly powerful technique for studying local thermogenesis of cells and helps to explore the relationship between cellular thermogenesis and cellular processes.
