The implementation of the field responsibility system liberated peasants from the land and started the differentiation of peasants＇ occupation. With the differentiation of peasants＇ occupation and income differentia...The implementation of the field responsibility system liberated peasants from the land and started the differentiation of peasants＇ occupation. With the differentiation of peasants＇ occupation and income differentiation, the functions of farmland also change accordingly. Based on the analysis of statistical data. the study found that over the past 30 years, the degree of occupational differentiation of peasants has become more and more complete, and income differentiation has also expanded. At the same time, the security function of traditional farmland is declining, but peasants pay more and more attention to the property function of farmland.展开更多
Background Preeclampsia （PE） is associated with abnormal fatty acid beta-oxidation （FAO）, especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is un...Background Preeclampsia （PE） is associated with abnormal fatty acid beta-oxidation （FAO）, especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts. Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyI-CoA dehydrogenase （LCHAD） protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated. Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression （P 〈0.05） but no significant difference in CPT1 protein expression （P 〉0.05）. Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased （P 〈0.05）. Intracellular lipid droplets deposition was negatively correlated with invasivenss （R=-0.745, P 〈0.05）. Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.展开更多
文摘The implementation of the field responsibility system liberated peasants from the land and started the differentiation of peasants＇ occupation. With the differentiation of peasants＇ occupation and income differentiation, the functions of farmland also change accordingly. Based on the analysis of statistical data. the study found that over the past 30 years, the degree of occupational differentiation of peasants has become more and more complete, and income differentiation has also expanded. At the same time, the security function of traditional farmland is declining, but peasants pay more and more attention to the property function of farmland.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 81241021） and Beijing Municipal Natural Science Foundation （No. 7132215）.
文摘Background Preeclampsia （PE） is associated with abnormal fatty acid beta-oxidation （FAO）, especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts. Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyI-CoA dehydrogenase （LCHAD） protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated. Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression （P 〈0.05） but no significant difference in CPT1 protein expression （P 〉0.05）. Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased （P 〈0.05）. Intracellular lipid droplets deposition was negatively correlated with invasivenss （R=-0.745, P 〈0.05）. Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.
