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Wu Weishan: Make "Steadfast to Cultural Confidence" Practicable
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作者 han yeting 《China & The World Cultural Exchange》 2017年第12期25-25,共1页
Instead of simply writing and drawing, singing and dancing, or purely writing pastoral poetry and expressing emotions, culture should reflect styles and features of the time and people's voices and should provide spi... Instead of simply writing and drawing, singing and dancing, or purely writing pastoral poetry and expressing emotions, culture should reflect styles and features of the time and people's voices and should provide spiritual impetus and intellectual support to the prosperity and rejuvenation of our nation, Literarv Artists need to have high cultural confidence to bear responsibility. Without confidence, how can they use their works to help others to become more confident? 展开更多
关键词 信心 文化 艺术家 工作帮
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酶法生产1,4-环己烷二甲胺 被引量:1
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作者 韩业挺 何志震 +4 位作者 魏婉清 宋伟 刘立明 朱萌 吴静 《生物工程学报》 CAS CSCD 北大核心 2024年第6期1882-1894,共13页
1,4-环己烷二甲胺(1,4-cyclohexanedimethylamine,1,4-BAC)作为重要的生物基材料单体,被广泛应用于有机合成、医药、化工和材料等多个领域。目前主要采用化学法合成,存在金属催化剂价格昂贵、反应条件苛刻和安全隐患等问题,亟须寻找更... 1,4-环己烷二甲胺(1,4-cyclohexanedimethylamine,1,4-BAC)作为重要的生物基材料单体,被广泛应用于有机合成、医药、化工和材料等多个领域。目前主要采用化学法合成,存在金属催化剂价格昂贵、反应条件苛刻和安全隐患等问题,亟须寻找更环保的合成替代方法。本研究利用大肠杆菌(Escherichia coli)来源的转氨酶(transaminase,Ec TA)、酿酒酵母(Saccharomyces cerevisiae)来源的谷氨酸脱氢酶(glutamate dehydrogenase,Sc Glu-DH)和博伊丁假丝酵母(Candida boidinii)来源的甲酸脱氢酶(formate dehydrogenase,Cb FDH)成功构建了双菌三酶级联转化1,4-环己烷二甲醛生成1,4-环己烷二甲胺的路径。基于结构指导下的蛋白质工程改造,获得了有益突变体Ec TAF91Y,其比酶活和kcat/Km较野生型分别提升了2.2倍和1.9倍。通过重组菌株的构建和反应条件的优化,在最优条件下40 g/L底物可生成(27.4±0.9)g/L产物,摩尔转化率为67.5%±2.1%。 展开更多
关键词 1 4-环己烷二甲胺 三酶级联 蛋白质工程改造 全细胞转化 1 4-环己烷二甲醛
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"Integrated Protection" Keeps Cultural Heritage "Alive"--The Establishment of Pilot Area for Cultural and Ecological Protection Solves the Problems of the Protection of Intangible Cultural Heritage (ICH)
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作者 han yeting 《China & The World Cultural Exchange》 2017年第3期38-39,共2页
In recent years, the Ministry of Cuhure has constantly issued advices requiring the pilot area construction to get close to people and life and to protect the ICH project as well as the cultural and ecological environ... In recent years, the Ministry of Cuhure has constantly issued advices requiring the pilot area construction to get close to people and life and to protect the ICH project as well as the cultural and ecological environment that breeds and nourishes ICH. Original residents and their lifestyles should be maintained in the renovation of ancient villages and old streets so that traditional villages and old streets within the pilot area won't become hollow sites with only buildings and shops and ICH won't lose the gene, environment and soil for inheritance. 展开更多
关键词 文化遗产保护 生态保护 综合保护 文化生态环境 物质 生活保障 生活方式 文化部
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10 Most Important People for China's Intangible Cultural Heritage of the Year 2018
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作者 han yeting 《China & The World Cultural Exchange》 2019年第2期26-27,共2页
The selection of China s Intangible Cultural Heritage Person of the Year is directed by the Intangible Cultural Heritage Department, Ministry of Culture and f ourism, organized and implemented by Guangming Daily and G... The selection of China s Intangible Cultural Heritage Person of the Year is directed by the Intangible Cultural Heritage Department, Ministry of Culture and f ourism, organized and implemented by Guangming Daily and Guangming Online. 展开更多
关键词 selection INTANGIBLE CULTURAL Guangming Online
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国粹让讽刺多了一份温柔力量——评京剧小戏《开市大吉》
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作者 韩业庭 《中国京剧》 2025年第12期62-64,F0003,共4页
“贾内行”“甄不懂”……光看人物名字,就颇有几分喜剧色彩。改编自刘宝瑞经典相声作品《假行家》的京剧小戏《开市大吉》,讲述了财主东家甄不懂、药铺掌柜贾内行以及医药外行陈皮匠之间令人啼笑皆非的闹剧故事。剧中,药铺掌柜贾内行... “贾内行”“甄不懂”……光看人物名字,就颇有几分喜剧色彩。改编自刘宝瑞经典相声作品《假行家》的京剧小戏《开市大吉》,讲述了财主东家甄不懂、药铺掌柜贾内行以及医药外行陈皮匠之间令人啼笑皆非的闹剧故事。剧中,药铺掌柜贾内行不懂任何医药知识,却敢坐堂问诊,并在老婆凤二娘和陈皮匠的配合下上演了一幕幕夸张、荒诞的问诊闹剧。更夸张、更荒诞的是,财主东家甄不懂却认为贾内行医术高明。当相声《假行家》脱下语言艺术的外衣,换上京剧的水袖与油彩,以《开市大吉》之名在小剧场舞台绽放时,一场关于传统与创新、荒诞与清醒的艺术实验,便在锣鼓点中拉开了帷幕。 展开更多
关键词 小戏 财主东家 医药外行 艺术实验 开市大吉 荒诞闹剧
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