Severe traumatic bone healing relies on the involvement of growth factors.However,excessive supplementation of growth factors can lead to ectopic ossification and inflammation.In this study,utilizing the neural regula...Severe traumatic bone healing relies on the involvement of growth factors.However,excessive supplementation of growth factors can lead to ectopic ossification and inflammation.In this study,utilizing the neural regulatory mechanism of bone regeneration,we have developed a multifunctional three dimensions(3D)printed scaffold containing both vasoactive intestinal peptide(VIP)and nerve growth factor(NGF)as an effective new method for achieving bone defect regeneration.The scaffold is provided by a controlled biodegradable and biomechanically matched poly(lactide-ethylene glycol-trimethylene carbonate)(PLTG),providing long-term support for the bone healing cycle.Factor loading is provided by peptide fiber-reinforced biomimetic antimicrobial extracellular matrix(ECM)(B-ECM)hydrogels with different release kinetics,the hydrogel guides rapid bone growth and resists bacterial infection at the early stage of healing.Physical and chemical characterization indicates that the scaffold has good structural stability and mechanical properties,providing an ideal 3D microenvironment for bone reconstruction.In the skull defect model,compared to releasing VIP or NGF alone,this drug delivery system can simulate a natural healing cascade of controllable release factors,significantly accelerating nerve/vascular bone regeneration.In conclusion,this study provides a promising strategy for implanting materials to repair bone defects by utilizing neuroregulatory mechanisms during bone regeneration.展开更多
Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wh...Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wheat(Triticum aestivum L.cv.Jingdong No.1).The cytoplasmic microtu-bules play an important role in cytoplasmic organization,cell division,and the correct transmission of genetic information in fission yeast cell.Using the fission yeast system in vivo experiments,overexpression of TaRAN/produced defective spindle microtubules,probably resulting in chromosome missegregation we reported previously.The microtubules of antisense TaRANI yeast cells were physically disrupted.This suggested that TaRAN/plays a role in mitotic spindle assembly and microtubule integrity and stability.Ultrastructural analysis under transmission electron microscope(TEM)showed abnormal nuclear membranes in the overexpression TaRAN/yeast cells,abnormal vacuole structures and disorganized membranes in the antisense TaRANI yeast cells.These results suggested that TaRANI was essential for all nucleocytoplasmic transport events.展开更多
Recently, graphene-based photodetectors have been rapidly developed. However, their photoresponsivities are generally low due to the weak optical absorption strength of graphene. In this paper, we fabricate photocondu...Recently, graphene-based photodetectors have been rapidly developed. However, their photoresponsivities are generally low due to the weak optical absorption strength of graphene. In this paper, we fabricate photoconductive multi-layer graphene（MLG） photodetectors on etched silicon-on-insulator substrates. A photoresponsivity exceeding 200 A·W-1is obtained, which enables most optoelectronic application. In addition, according to the analyses of the high photoresponsivity and long photoresponse time, we conclude that the working mechanism of the device is photoconductive effect. The process of photons conversion into conducting electrons is also described in detail. Finally, according to the distinct difference between the photoresponses at 1550 nm and 808 nm, we estimate that the position of the trapping energy is somewhere between 0.4 e V and 0.76 e V, higher than the Fermi energy of MLG. Our work paves a new way for fabricating the graphene photoconductive photodetectors.展开更多
目的探讨生境成像(habitat imaging,HI)无创定量可视化前列腺癌(prostate cancer,PCa)分区异质性并预测其危险度的可行性。材料与方法回顾性收集2018年1月至2024年8月在西京医院行多参数磁共振成像(multi-parameteric magnetic resonanc...目的探讨生境成像(habitat imaging,HI)无创定量可视化前列腺癌(prostate cancer,PCa)分区异质性并预测其危险度的可行性。材料与方法回顾性收集2018年1月至2024年8月在西京医院行多参数磁共振成像(multi-parameteric magnetic resonance imaging,mpMRI)扫描,包括扩散加权成像、体素内非相干运动加权成像和扩散峰度成像,并经根治性前列腺切除术(radical prostatectomy,RP)后病理证实为PCa的147例患者的临床及影像资料,以7∶3分为训练集和测试集。根据RP结果分为移行区(transition zone,TZ)和外周区(peripheral zone,PZ)PCa。整合每体素表观扩散系数(apparent diffusion coefficient,ADC)、灌注分数(perfusion fraction,f)和平均峰度(mean kurtosis,MK)值,划分生境亚区,生成生境地图,从临床、病理、影像多维度比较PZ和TZ PCa间差异。根据2019版国际泌尿病理协会(International Society of Urological Pathology,ISUP)指南,匹配生境地图与RP标本以评估各亚区ISUP分级,将患者分为低危组(ISUP≤2)和高危组(ISUP≥3)。logistic回归分析高危PCa相关特征并构建基于分区的HI(zone-based HI,zHI)-临床影像模型评估危险度,并评估模型效能。结果根据肘部法所示最佳聚类簇数划分3个生境亚区,生境1较生境2、3的ADC、f值更低,MK值更高。相较于TZ,PZ PCa患者临床、病理特征更恶,生境1占比更高。logistic回归分析示解剖分区(OR=3.50,95%CI:1.01~12.09)和生境1占比(OR=3.63,95%CI:1.37~9.62)是高危PCa的独立危险因素(P<0.05)。zHI-临床影像模型评估危险度在训练集和测试集的曲线下面积(area under the curve,AUC)分别为0.889(95%CI:0.822~0.955)和0.883(95%CI:0.740~0.925)。结论本研究多维度验证了PCa分区异质性,并基于解剖分区和HI特征构建模型,对PCa危险度的无创定量可视化预测具有增益效能。展开更多
基金supported by the Laboratory Open Fund of Key Technology and Materials in Minimally Invasive Spine Surgery(No.2024JZWC-YBA06)Medical and Industrial Cross Research Fundation of"Star of Jiaotong University"Program of Shanghai Jiao Tong University(Nos.YG2022ZD030,YG2021ZD34)+3 种基金Shanghai Jiao Tong University Institute of Minimally Invasive Surgery on Spine(No.2021JCPT03)Tongren Hospital Introduces the Talented Person Scientific Research Start Funds Subsidization Project(No.TR2022rc07)Tongren Hospital Top Priority Subject Project(No.tr2023xk01),Youth Innovation Promotion Association of CAS(No.2021264)Shanghai Natural Science Foundation(No.22ZR1469800).
文摘Severe traumatic bone healing relies on the involvement of growth factors.However,excessive supplementation of growth factors can lead to ectopic ossification and inflammation.In this study,utilizing the neural regulatory mechanism of bone regeneration,we have developed a multifunctional three dimensions(3D)printed scaffold containing both vasoactive intestinal peptide(VIP)and nerve growth factor(NGF)as an effective new method for achieving bone defect regeneration.The scaffold is provided by a controlled biodegradable and biomechanically matched poly(lactide-ethylene glycol-trimethylene carbonate)(PLTG),providing long-term support for the bone healing cycle.Factor loading is provided by peptide fiber-reinforced biomimetic antimicrobial extracellular matrix(ECM)(B-ECM)hydrogels with different release kinetics,the hydrogel guides rapid bone growth and resists bacterial infection at the early stage of healing.Physical and chemical characterization indicates that the scaffold has good structural stability and mechanical properties,providing an ideal 3D microenvironment for bone reconstruction.In the skull defect model,compared to releasing VIP or NGF alone,this drug delivery system can simulate a natural healing cascade of controllable release factors,significantly accelerating nerve/vascular bone regeneration.In conclusion,this study provides a promising strategy for implanting materials to repair bone defects by utilizing neuroregulatory mechanisms during bone regeneration.
文摘Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in cell cycle and whose loss affects many biological processes.We have identified cDNA of TaRAN/,a novel Ran GTPase homologous gene in wheat(Triticum aestivum L.cv.Jingdong No.1).The cytoplasmic microtu-bules play an important role in cytoplasmic organization,cell division,and the correct transmission of genetic information in fission yeast cell.Using the fission yeast system in vivo experiments,overexpression of TaRAN/produced defective spindle microtubules,probably resulting in chromosome missegregation we reported previously.The microtubules of antisense TaRANI yeast cells were physically disrupted.This suggested that TaRAN/plays a role in mitotic spindle assembly and microtubule integrity and stability.Ultrastructural analysis under transmission electron microscope(TEM)showed abnormal nuclear membranes in the overexpression TaRAN/yeast cells,abnormal vacuole structures and disorganized membranes in the antisense TaRANI yeast cells.These results suggested that TaRANI was essential for all nucleocytoplasmic transport events.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFB0402404)the High-Tech Research and Development Program of China(Grant Nos.2013AA031401,2015AA016902,and 2015AA016904)the National Natural Science Foundation of China(Grant Nos.61674136,61176053,61274069,and 61435002)
文摘Recently, graphene-based photodetectors have been rapidly developed. However, their photoresponsivities are generally low due to the weak optical absorption strength of graphene. In this paper, we fabricate photoconductive multi-layer graphene（MLG） photodetectors on etched silicon-on-insulator substrates. A photoresponsivity exceeding 200 A·W-1is obtained, which enables most optoelectronic application. In addition, according to the analyses of the high photoresponsivity and long photoresponse time, we conclude that the working mechanism of the device is photoconductive effect. The process of photons conversion into conducting electrons is also described in detail. Finally, according to the distinct difference between the photoresponses at 1550 nm and 808 nm, we estimate that the position of the trapping energy is somewhere between 0.4 e V and 0.76 e V, higher than the Fermi energy of MLG. Our work paves a new way for fabricating the graphene photoconductive photodetectors.
文摘目的探讨生境成像(habitat imaging,HI)无创定量可视化前列腺癌(prostate cancer,PCa)分区异质性并预测其危险度的可行性。材料与方法回顾性收集2018年1月至2024年8月在西京医院行多参数磁共振成像(multi-parameteric magnetic resonance imaging,mpMRI)扫描,包括扩散加权成像、体素内非相干运动加权成像和扩散峰度成像,并经根治性前列腺切除术(radical prostatectomy,RP)后病理证实为PCa的147例患者的临床及影像资料,以7∶3分为训练集和测试集。根据RP结果分为移行区(transition zone,TZ)和外周区(peripheral zone,PZ)PCa。整合每体素表观扩散系数(apparent diffusion coefficient,ADC)、灌注分数(perfusion fraction,f)和平均峰度(mean kurtosis,MK)值,划分生境亚区,生成生境地图,从临床、病理、影像多维度比较PZ和TZ PCa间差异。根据2019版国际泌尿病理协会(International Society of Urological Pathology,ISUP)指南,匹配生境地图与RP标本以评估各亚区ISUP分级,将患者分为低危组(ISUP≤2)和高危组(ISUP≥3)。logistic回归分析高危PCa相关特征并构建基于分区的HI(zone-based HI,zHI)-临床影像模型评估危险度,并评估模型效能。结果根据肘部法所示最佳聚类簇数划分3个生境亚区,生境1较生境2、3的ADC、f值更低,MK值更高。相较于TZ,PZ PCa患者临床、病理特征更恶,生境1占比更高。logistic回归分析示解剖分区(OR=3.50,95%CI:1.01~12.09)和生境1占比(OR=3.63,95%CI:1.37~9.62)是高危PCa的独立危险因素(P<0.05)。zHI-临床影像模型评估危险度在训练集和测试集的曲线下面积(area under the curve,AUC)分别为0.889(95%CI:0.822~0.955)和0.883(95%CI:0.740~0.925)。结论本研究多维度验证了PCa分区异质性,并基于解剖分区和HI特征构建模型,对PCa危险度的无创定量可视化预测具有增益效能。
