OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective m...OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective mechanism of WPBA in stroke. METHODS： Adult male Sprague Dawley （n = 32） rats were randomly divided into four groups of eight animals each： WPBA-thalamus group （WT）, WPBA-caudate nucleus group （WC）, sham-control thalamus group （ST） and sham-control caudate nucleus group （SC）. Animals in the WT and WC groups received 2 I-μL of the extracellular tracer gadolinium-diethylene triamine pentaacetic acid （Gd-DTPA） injected into the thalamus or caudate nucleus, respectively, and 12 Jing-well points in the distal ends of the rats＇ digits were used for WPBA. Although 2 μL of Gd-DTPA was injected into the thalamus or caudate nucleus, respectively, for animals in the two sham groups （ST and SC）, no acupuncture or bloodletting was performed. Brain extracellular space and interstitial fluid flow parameters were measured using Gd-DTPA-enhanced magnetic reso- nance imaging. RESULTS： The brain interstitial fluid flow speed was decreased in the thalamus after WPBA, with a significantly lower Gd-DTPA clearance rate and longer half-life of Gd-DTPA in the thalamus of treated rats than those in sham-control rats [WPBA-treated rats＇clearance rate, （7.47 ± 3.15） x 10^-5/s （P = 0.009）; half-life, （1.52 ± 0.13） h, P = 0.000]. By contrast, no significant changes in brain extracellular space and interstitial fluid flow parameters were detected in the caudate nucleus after WPBA （P = 0.649）. In addi- tion, no differences in the morphology of the brain extracellular space or the final distribution of the traced brain interstitial fluid were demonstrated be- tween theWT and WC groups （P = 0.631, P = 0.970, respectively）. CONCLUSION： The WPBA decreased the speed of the local thalamic ISF flow in rats, which is assumed to be a beneficial protection by down-modulated the metabolic rate of the attacked neurons under stroke.展开更多
The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morpholo...The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morphology and water status in a living plant bulb and aid in investigating release factors. To evaluate whether MRI could be used to detect intra-bulb metabolic changes during the dormant period in Oriental Lilies (Lilium 'Sorbonne'), a series of MRI and sugar concentration measurements were performed weekly on bulbs stored for 11 weeks at 4°C. The image quality of intra-bulb structure obtained using T 1-weighted imaging was superior to that obtained using T 2 -weighted imaging and had a higher signal-to-noise ratio (0.97±0.01). Magnetization transfer ratio values for the bud and basal plate declined during the first eight weeks of cold storage (P>0.05), and were well correlated with concentration of soluble sugar in the bud (R 2 =0.95) and basal plate (R 2 =0.93). Thus, MRI can serve as a valuable tool for observation and analysis of dynamic morphological and metabolic changes in vivo during dormancy release. This information is potentially useful as a guide in the improvement of horticultural product quality.展开更多
基金Supported by the National Natural Science Foundation of China(Related Mechanisms and Basic Research of Citicolinein Treatment of Ultra-Early Cerebral Ischemia Delivery via Brain Interstitial,No.81171080)the National Key Technology R&D Program for the 12th Five-year Plan(Advanced Cancer Treatment Equipment and Materials Development,No.2012BAI15B009)+1 种基金National Key Developmental Program for Scientific Instruments and Equipment(Study of Delivery via Brain Interstitial Based on Multimodal Molecular Imaging Technology,No.2011YQ030114)a Seeding Grant for Medicine and Information Sciences of Peking University(Disciplinarian and Mechanism Research of Information Transmission and Interaction between Somatosensory Stimulation and Brain Tissue Channel,No.2014-MI-12)
文摘OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective mechanism of WPBA in stroke. METHODS： Adult male Sprague Dawley （n = 32） rats were randomly divided into four groups of eight animals each： WPBA-thalamus group （WT）, WPBA-caudate nucleus group （WC）, sham-control thalamus group （ST） and sham-control caudate nucleus group （SC）. Animals in the WT and WC groups received 2 I-μL of the extracellular tracer gadolinium-diethylene triamine pentaacetic acid （Gd-DTPA） injected into the thalamus or caudate nucleus, respectively, and 12 Jing-well points in the distal ends of the rats＇ digits were used for WPBA. Although 2 μL of Gd-DTPA was injected into the thalamus or caudate nucleus, respectively, for animals in the two sham groups （ST and SC）, no acupuncture or bloodletting was performed. Brain extracellular space and interstitial fluid flow parameters were measured using Gd-DTPA-enhanced magnetic reso- nance imaging. RESULTS： The brain interstitial fluid flow speed was decreased in the thalamus after WPBA, with a significantly lower Gd-DTPA clearance rate and longer half-life of Gd-DTPA in the thalamus of treated rats than those in sham-control rats [WPBA-treated rats＇clearance rate, （7.47 ± 3.15） x 10^-5/s （P = 0.009）; half-life, （1.52 ± 0.13） h, P = 0.000]. By contrast, no significant changes in brain extracellular space and interstitial fluid flow parameters were detected in the caudate nucleus after WPBA （P = 0.649）. In addi- tion, no differences in the morphology of the brain extracellular space or the final distribution of the traced brain interstitial fluid were demonstrated be- tween theWT and WC groups （P = 0.631, P = 0.970, respectively）. CONCLUSION： The WPBA decreased the speed of the local thalamic ISF flow in rats, which is assumed to be a beneficial protection by down-modulated the metabolic rate of the attacked neurons under stroke.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 200903020)
文摘The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morphology and water status in a living plant bulb and aid in investigating release factors. To evaluate whether MRI could be used to detect intra-bulb metabolic changes during the dormant period in Oriental Lilies (Lilium 'Sorbonne'), a series of MRI and sugar concentration measurements were performed weekly on bulbs stored for 11 weeks at 4°C. The image quality of intra-bulb structure obtained using T 1-weighted imaging was superior to that obtained using T 2 -weighted imaging and had a higher signal-to-noise ratio (0.97±0.01). Magnetization transfer ratio values for the bud and basal plate declined during the first eight weeks of cold storage (P>0.05), and were well correlated with concentration of soluble sugar in the bud (R 2 =0.95) and basal plate (R 2 =0.93). Thus, MRI can serve as a valuable tool for observation and analysis of dynamic morphological and metabolic changes in vivo during dormancy release. This information is potentially useful as a guide in the improvement of horticultural product quality.
