BACKGROUND Endogenous regeneration of pancreatic isletβ-cells is a path to cure both type 1 and advanced type 2 diabetes.Pancreatic cancer cell line-1(PANC-1),a human pancreatic islet progenitor cell line,can be indu...BACKGROUND Endogenous regeneration of pancreatic isletβ-cells is a path to cure both type 1 and advanced type 2 diabetes.Pancreatic cancer cell line-1(PANC-1),a human pancreatic islet progenitor cell line,can be induced by trypsin to differentiate into insulin-secreting islet-like aggregates(ILAs).However,the underlying mechanism has not been explored.AIM To explore the mechanism and signaling pathway of trypsin-induced differentiation of islet progenitor cells into insulin-secreting cells.METHODS PANC-1 cells were induced by trypsin to form ILAs and differentiate into insulinsecreting cells.Clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9 knockout and small interfering RNA knockdown techniques were used to investigate membrane proteins and downstream signaling pathways involved in the process.RESULTS The extracellular domain of membrane receptor E-cadherin hydrolyzed by trypsin induced the aggregation of PANC-1 cells and stimulated E-cadherin-recruited casein kinase-1γ3,which specifically phosphorylated the Ser655/Thr658 site ofα-catenin in the cadherin-catenin complex,participating in the process of PANC-1 differentiation and affecting the maturation of differentiated ILAs.CONCLUSION The current study reveals the mechanism by which trypsin promotes PANC-1 cell differentiation into islet-like cells,providing a novel approach for endogenous isletβ-cell regeneration.展开更多
Objective:To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA sequencing.Methods: Mice were randomly assigned to control,model,GSQ low-dose(GSQ...Objective:To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA sequencing.Methods: Mice were randomly assigned to control,model,GSQ low-dose(GSQL),GSQ medium-dose(GSQM),GSQ high-dose(GSQH),and lacidophilin tablets(LAB)groups,with each group containing 10 mice.A food stagnation and internal heat mouse model was established through intragastric administration of a mixture of beeswax and olive oil(1:15).The control group was administered normal saline,and the model group was administered beeswax and olive oil to maintain a state.The GSQL(2 g/kg),GSQM(4 g/kg),GSQH(8 g/kg),and LAB groups(0.625 g/kg)were administered corresponding drugs for 5 d.After administration,16S rDNA sequencing was performed to assess gut microbiota in mouse fecal samples.Results: The model group exhibited significant intestinal flora changes.Following GSQ administration,the abundance and diversity index of the intestinal flora increased significantly,the number of bacterial species was regulated,andαandβdiversity were improved.GSQ administration increased the abundance of probiotics,including Clostridia,Lachnospirales,and Lactobacillus,whereas the abundance of conditional pathogenic bacteria,such as Allobaculum,Erysipelotrichaceae,and Bacteroides decreased.Functional prediction analysis indicated that the pathogenesis of food stagnation and GSQ intervention were primarily associated with carbohydrate,lipid,and amino acid metabolism,among other metabolic pathways.Conclusion: The digestive mechanism of GSQ may be attributed to its role in restoring diversity and abundance within the intestinal flora,thereby improving the composition and structure of the intestinal flora in mice and subsequently influencing the regulation of metabolic pathways.展开更多
In the face of multiple challenges brought by the changes of global climate and environment,developing clean energy and updating green energy storage equipment are important ways to achieve carbon peak and carbon neut...In the face of multiple challenges brought by the changes of global climate and environment,developing clean energy and updating green energy storage equipment are important ways to achieve carbon peak and carbon neutrality.Aqueous batteries have become a research hotspot due to their advantages of using the multivalent charge carrier,high ionic conductivity,environmental friendliness and cost effectiveness.In this work,the Cu_(2)Se@C(Cu_(2)Se coated on carbon clothes)thin film with a three-dimensional braided structure is fabricated by a simple electrochemical deposition method for Cu^(2+)storage for the first time.Compared with the commercial Cu_(2)Se powder,the well-designed Cu_(2)Se@C film shows enhanced specific capacity(640 mAh/g at 0.5 A/g)and rate performance(542 mAh/g at 5 A/g)as well as superior cycling stability(82.7%capacity retention after 1000 cycles at 1 A/g).The Cu^(2+)storage mechanism of the Cu_(2)Se@C electrode is based on a reversible phase transition process of Cu_(2)Se←→Cu_(2-x)Se←→CuSe←→CuSe_(2).In kinetic characteristic analysis,the Cu_(2)Se@C electrode demonstrates faster Cu^(2+)diffusion in discharge process than charge process resulting from the phase transition and the variation of interplanar spacing.This work highlights a facile one-piece design strategy and opens a new gateway for the exploration of advanced aqueous energy storage systems.展开更多
Objective:To study the application effect of the Enhanced Recovery After Surgery(ERAS)model in patients undergoing spinal fracture surgery.Methods:A randomized controlled trial was designed,and 86 patients undergoing ...Objective:To study the application effect of the Enhanced Recovery After Surgery(ERAS)model in patients undergoing spinal fracture surgery.Methods:A randomized controlled trial was designed,and 86 patients undergoing spinal fracture surgery were randomly divided into the ERAS group and the conventional care group.Postoperative recovery outcomes of the two groups were compared.Results:The ERAS group showed better outcomes in terms of postoperative pain scores,activities of daily living,length of hospital stay,and adherence to rehabilitation training compared to the conventional care group,with shorter hospital stays and lower medical expenses(P<0.05).Conclusion:The ERAS model significantly improves the postoperative recovery quality of patients undergoing spinal fracture surgery,reduces hospital stay and medical costs,and increases patient satisfaction.展开更多
基金Supported by the National Natural Science Foundation of China,No.82073908.
文摘BACKGROUND Endogenous regeneration of pancreatic isletβ-cells is a path to cure both type 1 and advanced type 2 diabetes.Pancreatic cancer cell line-1(PANC-1),a human pancreatic islet progenitor cell line,can be induced by trypsin to differentiate into insulin-secreting islet-like aggregates(ILAs).However,the underlying mechanism has not been explored.AIM To explore the mechanism and signaling pathway of trypsin-induced differentiation of islet progenitor cells into insulin-secreting cells.METHODS PANC-1 cells were induced by trypsin to form ILAs and differentiate into insulinsecreting cells.Clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9 knockout and small interfering RNA knockdown techniques were used to investigate membrane proteins and downstream signaling pathways involved in the process.RESULTS The extracellular domain of membrane receptor E-cadherin hydrolyzed by trypsin induced the aggregation of PANC-1 cells and stimulated E-cadherin-recruited casein kinase-1γ3,which specifically phosphorylated the Ser655/Thr658 site ofα-catenin in the cadherin-catenin complex,participating in the process of PANC-1 differentiation and affecting the maturation of differentiated ILAs.CONCLUSION The current study reveals the mechanism by which trypsin promotes PANC-1 cell differentiation into islet-like cells,providing a novel approach for endogenous isletβ-cell regeneration.
基金supported by the National Natural Science Foundation of China(81872995).
文摘Objective:To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA sequencing.Methods: Mice were randomly assigned to control,model,GSQ low-dose(GSQL),GSQ medium-dose(GSQM),GSQ high-dose(GSQH),and lacidophilin tablets(LAB)groups,with each group containing 10 mice.A food stagnation and internal heat mouse model was established through intragastric administration of a mixture of beeswax and olive oil(1:15).The control group was administered normal saline,and the model group was administered beeswax and olive oil to maintain a state.The GSQL(2 g/kg),GSQM(4 g/kg),GSQH(8 g/kg),and LAB groups(0.625 g/kg)were administered corresponding drugs for 5 d.After administration,16S rDNA sequencing was performed to assess gut microbiota in mouse fecal samples.Results: The model group exhibited significant intestinal flora changes.Following GSQ administration,the abundance and diversity index of the intestinal flora increased significantly,the number of bacterial species was regulated,andαandβdiversity were improved.GSQ administration increased the abundance of probiotics,including Clostridia,Lachnospirales,and Lactobacillus,whereas the abundance of conditional pathogenic bacteria,such as Allobaculum,Erysipelotrichaceae,and Bacteroides decreased.Functional prediction analysis indicated that the pathogenesis of food stagnation and GSQ intervention were primarily associated with carbohydrate,lipid,and amino acid metabolism,among other metabolic pathways.Conclusion: The digestive mechanism of GSQ may be attributed to its role in restoring diversity and abundance within the intestinal flora,thereby improving the composition and structure of the intestinal flora in mice and subsequently influencing the regulation of metabolic pathways.
基金partly supported by the National Natural Science Foundation of China(No.51972108)Distinguished Young Scientists of Hunan Province(No.2022JJ10024)+1 种基金Natural Science Foundation of Hunan Province(No.2021JJ30216)Key Projects of Hunan Provincial Education Department(No.22A0412)。
文摘In the face of multiple challenges brought by the changes of global climate and environment,developing clean energy and updating green energy storage equipment are important ways to achieve carbon peak and carbon neutrality.Aqueous batteries have become a research hotspot due to their advantages of using the multivalent charge carrier,high ionic conductivity,environmental friendliness and cost effectiveness.In this work,the Cu_(2)Se@C(Cu_(2)Se coated on carbon clothes)thin film with a three-dimensional braided structure is fabricated by a simple electrochemical deposition method for Cu^(2+)storage for the first time.Compared with the commercial Cu_(2)Se powder,the well-designed Cu_(2)Se@C film shows enhanced specific capacity(640 mAh/g at 0.5 A/g)and rate performance(542 mAh/g at 5 A/g)as well as superior cycling stability(82.7%capacity retention after 1000 cycles at 1 A/g).The Cu^(2+)storage mechanism of the Cu_(2)Se@C electrode is based on a reversible phase transition process of Cu_(2)Se←→Cu_(2-x)Se←→CuSe←→CuSe_(2).In kinetic characteristic analysis,the Cu_(2)Se@C electrode demonstrates faster Cu^(2+)diffusion in discharge process than charge process resulting from the phase transition and the variation of interplanar spacing.This work highlights a facile one-piece design strategy and opens a new gateway for the exploration of advanced aqueous energy storage systems.
文摘Objective:To study the application effect of the Enhanced Recovery After Surgery(ERAS)model in patients undergoing spinal fracture surgery.Methods:A randomized controlled trial was designed,and 86 patients undergoing spinal fracture surgery were randomly divided into the ERAS group and the conventional care group.Postoperative recovery outcomes of the two groups were compared.Results:The ERAS group showed better outcomes in terms of postoperative pain scores,activities of daily living,length of hospital stay,and adherence to rehabilitation training compared to the conventional care group,with shorter hospital stays and lower medical expenses(P<0.05).Conclusion:The ERAS model significantly improves the postoperative recovery quality of patients undergoing spinal fracture surgery,reduces hospital stay and medical costs,and increases patient satisfaction.
