OBJECTIVE:To study the anaphylaxis of Qingkailing injection(QI) and its components.METHODS:Experimental anaphylactoid and allergic reactions were used.Changes in the behaviors of Beagles and serum levels of histamine,...OBJECTIVE:To study the anaphylaxis of Qingkailing injection(QI) and its components.METHODS:Experimental anaphylactoid and allergic reactions were used.Changes in the behaviors of Beagles and serum levels of histamine,immunoglobulin(Ig)E,IgG,IgM,eosinophil cationic protein(ECP),and interleukin(IL)-4,as well as blood pressure,after injecting QI and its components on the forelimb veins of Beagles were observed.RESULTS:According to comprehensive determination of abnormal behavior scores and changes in serum levels of histamine,IgE,IgG,IgM,ECP,and IL-4,as well as in blood pressure,radix isatidis and hyodeoxycholic acid caused anaphylactoid reactions,and honeysuckle,radix isatidis,hydrolysate,cholic acid and Gardenia jasminoides caused allergic reactions.The anaphylaxis of QI involved anaphylactoid and allergic reactions.CONCLUSION:QI and its components need to be refined further to improve the safety,efficacy,and quality of its use in clinical settings.展开更多
The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical...The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical c-fos gene expression was determined using in situ hybridization. Results showed that 3＇-methoxy-puerarin reduced neurological deficit scores, cerebral infarcted zone and water content of brain tissues, dramatically increased the activity of catalase and glutathione peroxidase in the ischemia zone of the hippocampus, increased the activity of catalase in the cortex, decreased lipid peroxide and lactic acid contents in the hippocampus and cerebral cortex, and down-regulated c-fos gene expression in brain ischemic rats. Results demonstrated that 3＇-methoxy-puerarin exhibited cerebroprotective effects against focal brain ischemia, which involved c-fos gene expression.展开更多
A test strip was developed to rapidly detect fluoroquinolones in foods by colloidal gold immunochromatography method in combination with a food safety analyzer. The results showed that the test strip has the detection...A test strip was developed to rapidly detect fluoroquinolones in foods by colloidal gold immunochromatography method in combination with a food safety analyzer. The results showed that the test strip has the detection sensitivity of 20 μg/L for enrofloxacin, sarafloxacin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxacin, flumequine and danofloxacin, and the detection sensitivity of 40 μg/L for enoxacin and oxolinic acid. The test strip consumes only 10 min for detection, and the false positive rate and the false negative rate are both zero. The test strip can accurately, reliably and easily detect residual fluoroquinolones in foods, and is suitable for on-site detection of a large number of samples.展开更多
Background:Diabetic cardiomyopathy(DCM)is a common heart problem in people with diabetes.It is a big public health problem that needs to be treated effectively.Shengmai San(SMS)can be used to treat diabetic cardiomyop...Background:Diabetic cardiomyopathy(DCM)is a common heart problem in people with diabetes.It is a big public health problem that needs to be treated effectively.Shengmai San(SMS)can be used to treat diabetic cardiomyopathy,coronary heart disease,and heart damage,but it is unclear whether it can protect the calcium balance of diabetic cardiomyopathy in the early stages.Objective:To investigate the regulatory mechanism of SMS on sarcoplasmic reticulum calcium transport in diabetic mice.Methods:Eight-week-old db/db mouse models were randomly divided into a diabetic model group(model),SMS intervention group(SMS),and CaMKⅡ inhibitor group(KN-93).Age-matched db/m mice were used as a control group.After 8 weeks of treatment,cardiac function and morphology were assessed,and calcium transients in cardiomyocytes and sarcoplasmic reticulum calcium transport were detected.Western blot was used to detect the expression of CaMKⅡ and proteins related to myocardial calcium transport.Results:In 16-week-old db/db mice,there was no obvious abnormality in myocardial contractile function,the ventricular wall was not thickened,and the cross-sectional area of cardiomyocytes was not significantly enlarged.Compared with the model group,in the SMS group,the calcium transient amplitude was increased,the calcium content of the sarcoplasmic reticulum was upregulated,the calcium elimination time constant was decreased,calcium elimination was accelerated,and the proportion of calcium released from the sarcoplasmic reticulum during the calcium transient was increased.At the same time,SMS could reduce cytosolic free calcium in diabetic mice,and its possible mechanism was to reduce calcium leakage from the sarcoplasmic reticulum,and its effect was similar to that of the KN93 group.The expressions of CaMKⅡ protein and p-CaMKⅡ protein were significantly upregulated in the model group,while SMS could significantly downregulate the expressions of both.Conclusions:SMS can reduce sarcoplasmic reticulum calcium leak and protect the calcium homeostasis of diabetic myocardium.Its mechanism may be by reducing the expression of CaMKⅡ protein and the phosphorylation level of CaMKⅡ protein.展开更多
基金Supported by National Science and Technology Major Projects(No.2010ZX09502-002)
文摘OBJECTIVE:To study the anaphylaxis of Qingkailing injection(QI) and its components.METHODS:Experimental anaphylactoid and allergic reactions were used.Changes in the behaviors of Beagles and serum levels of histamine,immunoglobulin(Ig)E,IgG,IgM,eosinophil cationic protein(ECP),and interleukin(IL)-4,as well as blood pressure,after injecting QI and its components on the forelimb veins of Beagles were observed.RESULTS:According to comprehensive determination of abnormal behavior scores and changes in serum levels of histamine,IgE,IgG,IgM,ECP,and IL-4,as well as in blood pressure,radix isatidis and hyodeoxycholic acid caused anaphylactoid reactions,and honeysuckle,radix isatidis,hydrolysate,cholic acid and Gardenia jasminoides caused allergic reactions.The anaphylaxis of QI involved anaphylactoid and allergic reactions.CONCLUSION:QI and its components need to be refined further to improve the safety,efficacy,and quality of its use in clinical settings.
文摘The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical c-fos gene expression was determined using in situ hybridization. Results showed that 3＇-methoxy-puerarin reduced neurological deficit scores, cerebral infarcted zone and water content of brain tissues, dramatically increased the activity of catalase and glutathione peroxidase in the ischemia zone of the hippocampus, increased the activity of catalase in the cortex, decreased lipid peroxide and lactic acid contents in the hippocampus and cerebral cortex, and down-regulated c-fos gene expression in brain ischemic rats. Results demonstrated that 3＇-methoxy-puerarin exhibited cerebroprotective effects against focal brain ischemia, which involved c-fos gene expression.
基金Supported by Beijing Training Project for the Leading Talents in S&T(Z171100001117158)
文摘A test strip was developed to rapidly detect fluoroquinolones in foods by colloidal gold immunochromatography method in combination with a food safety analyzer. The results showed that the test strip has the detection sensitivity of 20 μg/L for enrofloxacin, sarafloxacin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxacin, flumequine and danofloxacin, and the detection sensitivity of 40 μg/L for enoxacin and oxolinic acid. The test strip consumes only 10 min for detection, and the false positive rate and the false negative rate are both zero. The test strip can accurately, reliably and easily detect residual fluoroquinolones in foods, and is suitable for on-site detection of a large number of samples.
基金supported by the Fundamental Research Funds for the Central Public Welfare Research Institutes(JBGS2021006)the National Natural Science Foundation of China(82004244)the Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical Sciences(NLTS2025009).
文摘Background:Diabetic cardiomyopathy(DCM)is a common heart problem in people with diabetes.It is a big public health problem that needs to be treated effectively.Shengmai San(SMS)can be used to treat diabetic cardiomyopathy,coronary heart disease,and heart damage,but it is unclear whether it can protect the calcium balance of diabetic cardiomyopathy in the early stages.Objective:To investigate the regulatory mechanism of SMS on sarcoplasmic reticulum calcium transport in diabetic mice.Methods:Eight-week-old db/db mouse models were randomly divided into a diabetic model group(model),SMS intervention group(SMS),and CaMKⅡ inhibitor group(KN-93).Age-matched db/m mice were used as a control group.After 8 weeks of treatment,cardiac function and morphology were assessed,and calcium transients in cardiomyocytes and sarcoplasmic reticulum calcium transport were detected.Western blot was used to detect the expression of CaMKⅡ and proteins related to myocardial calcium transport.Results:In 16-week-old db/db mice,there was no obvious abnormality in myocardial contractile function,the ventricular wall was not thickened,and the cross-sectional area of cardiomyocytes was not significantly enlarged.Compared with the model group,in the SMS group,the calcium transient amplitude was increased,the calcium content of the sarcoplasmic reticulum was upregulated,the calcium elimination time constant was decreased,calcium elimination was accelerated,and the proportion of calcium released from the sarcoplasmic reticulum during the calcium transient was increased.At the same time,SMS could reduce cytosolic free calcium in diabetic mice,and its possible mechanism was to reduce calcium leakage from the sarcoplasmic reticulum,and its effect was similar to that of the KN93 group.The expressions of CaMKⅡ protein and p-CaMKⅡ protein were significantly upregulated in the model group,while SMS could significantly downregulate the expressions of both.Conclusions:SMS can reduce sarcoplasmic reticulum calcium leak and protect the calcium homeostasis of diabetic myocardium.Its mechanism may be by reducing the expression of CaMKⅡ protein and the phosphorylation level of CaMKⅡ protein.
