The presence of the Enterobacter cloacae complex(ECC)in natural water bodies poses potential public health risks because these bacteria can enter humans from the environment within the“One Health”framework.Neverthel...The presence of the Enterobacter cloacae complex(ECC)in natural water bodies poses potential public health risks because these bacteria can enter humans from the environment within the“One Health”framework.Nevertheless,there is a notable deficiency in the isolation and knowledge of carbapenem-and polymyxin-resistant ECC strains from natural water bodies.In this research,48 ECC strains were isolated from natural water bodies.Of these,eight strains were resistant to carbapenems(imipenem)and polymyxins(polymyxin B and colistin sulfate)and were classified as multi-locus sequence typing 13.Comparative genomic analysis identified bla_(CMY-63),pmrE,pmrF,and various efflux pump genes as key genetic determinants of co-resistance to carbapenems and polymyxins.Notably,these strains exhibited relatively high virulence,with cell death rates ranging from 57%to 84%.They also exhibited virulence against Galleria mellonella.This research sheds light on the potential health risks of carbapenem-and polymyxin-resistant ECC strains displaying virulence in natural water bodies.展开更多
文摘The presence of the Enterobacter cloacae complex(ECC)in natural water bodies poses potential public health risks because these bacteria can enter humans from the environment within the“One Health”framework.Nevertheless,there is a notable deficiency in the isolation and knowledge of carbapenem-and polymyxin-resistant ECC strains from natural water bodies.In this research,48 ECC strains were isolated from natural water bodies.Of these,eight strains were resistant to carbapenems(imipenem)and polymyxins(polymyxin B and colistin sulfate)and were classified as multi-locus sequence typing 13.Comparative genomic analysis identified bla_(CMY-63),pmrE,pmrF,and various efflux pump genes as key genetic determinants of co-resistance to carbapenems and polymyxins.Notably,these strains exhibited relatively high virulence,with cell death rates ranging from 57%to 84%.They also exhibited virulence against Galleria mellonella.This research sheds light on the potential health risks of carbapenem-and polymyxin-resistant ECC strains displaying virulence in natural water bodies.
