The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide dive...The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.展开更多
Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and struct...Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and structured optical field engineering.The exploration of MDMUXs employing cascaded resonant couplers has garnered significant attention owing to their scalability,exceptional integration capabilities,and the anticipated low insertion loss.In this work,we present the successful realization of high-quality orbital angular momentum MDMUX corresponding to topological charges 0,±1,and±2,achieved through the utilization of cascaded fused-biconical tapered couplers.Notably,the measured insertion losses at 1550 nm exhibit remarkable minimal values:0.31,0.10,and 0.64 dB,respectively.Furthermore,the 80%efficiency bandwidths exceed 106,174,and 174 nm for these respective modes.The MDMUX is composed of precisionmanufactured high-quality mode selective couplers(MSCs).Utilizing a proposed supermode propagation method based on mode composition analysis,we precisely describe the operational characteristics of MSCs.Building upon this comprehensive understanding,we embark on a pioneering analysis elucidating the influence of MSC cascading order on the performance of MDMUXs.Our theoretical investigation substantiates that when constructing MDMUXs,MSCs should adhere to a specific cascading sequence.展开更多
The optical vortex interferometer(OVI)has been found to have extensive applications in precision metrology due to its ultrahigh sensitivity.However,most current implementations rely on free-space optical components,pa...The optical vortex interferometer(OVI)has been found to have extensive applications in precision metrology due to its ultrahigh sensitivity.However,most current implementations rely on free-space optical components,particularly requiring beam splitter cubes to combine sensing and reference beams.This inevitably necessitates complex coaxial alignment of optical paths,where tilt,divergence,and centroid misalignment introduce significant challenges in phase demodulation.This paper presents an all-fiber OVI where beams interfere through a mode selective coupler.The orbital angular momentum order difference,which directly determines the sensitivity of the OVI,is reduced to one.A novel,to our knowledge,measurement and phase demodulation methodology is developed for the optical fields generated by the proposed interferometer,accompanied by systematic analysis of error sources and their magnitudes.The proposed OVI was applied to temperature sensing,achieving a sensitivity of 44.19 rad∕(℃·m),which is 2-3 orders of magnitude higher than that of conventional methods.The proposed OVI and corresponding demodulation approach show promising potential for ultrahigh-precision sensing applications.展开更多
Broadband mode converters are essential devices for space-division and wavelength-division multiplexing systems.There are great challenges in the generation of higher-order modes above the third order with low loss an...Broadband mode converters are essential devices for space-division and wavelength-division multiplexing systems.There are great challenges in the generation of higher-order modes above the third order with low loss and high mode purity employing all-fiber devices.In this paper,an all-fiber LP_(41)mode converter is proposed and fabricated by tapering a nine-core single-mode fiber bundle.Experimental results indicate that this all-fiber LP_(41)mode converter is low-loss,high-purity,and ultrabroadband.The insertion loss is less than 0.4 dB.The purity of odd LP_(41)at 1310 nm is 95.09%,and the operating bandwidth exceeds 280 nm.展开更多
基金supported by the National Natural Science Foundation(82130101)the Major Scientific and Technological Projects of Guangdong Province(2023B1111050008)the Post Scientist Fund awarded by the Chinese Academy of Traditional Chinese Medicine(ZZ13035-02)to Shuwen Liu.Regional Joint Foundation of Basic and Applied Basic Research in Guangdong Province(2023A1515111165)to Chan Yang.
文摘The ongoing coronavirus epidemic,including the novel coronavirus(SARS-CoV-2),continues to pose a significant threat to global public health.Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions.This study identified sorting nexin 10(SNX10)as a facilitator of replication of human coronavirus OC43(HCoV-OC43),underscoring its potential as a novel antiviral target.The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro.Immunoprecipitation-mass spectrometry(IP-MS)analysis identified the adaptor protein complex 2 subunitμ1(AP2M1)as a direct interactor of SNX10.Specifically,SNX10 facilitates phosphorylation of the AP2M1,thereby enhancing clathrin-mediated viral endocytosis.Furthermore,subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells.Conversely,the reconstitution of SNX10 fully restored viral entry,thereby confirming the critical and indispensable role of SNX10 in pathogen internalization.Simultaneously,SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels,which in turn facilitated the release of the viral genome.Notably,the ablation of SNX10 was found to trigger autophagy activation during infection,thereby maintaining intracellular homeostasis.Additionally,it exerted autonomous antiviral effects through lysosomal degradation pathways.Collectively,these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization,viral genomic release,and hostpathogen equilibrium.
基金supported by the National Key Research and Development Program of China (Grant No.2018YFB1801802)the National Natural Science Foundation of China (Grant Nos.62375143 and 61835006).
文摘Mode-division multiplexers(MDMUXs)play a pivotal role in enabling the manipulation of an arbitrary optical state within few-mode fibers,offering extensive utility in the fields of mode-division multiplexing and structured optical field engineering.The exploration of MDMUXs employing cascaded resonant couplers has garnered significant attention owing to their scalability,exceptional integration capabilities,and the anticipated low insertion loss.In this work,we present the successful realization of high-quality orbital angular momentum MDMUX corresponding to topological charges 0,±1,and±2,achieved through the utilization of cascaded fused-biconical tapered couplers.Notably,the measured insertion losses at 1550 nm exhibit remarkable minimal values:0.31,0.10,and 0.64 dB,respectively.Furthermore,the 80%efficiency bandwidths exceed 106,174,and 174 nm for these respective modes.The MDMUX is composed of precisionmanufactured high-quality mode selective couplers(MSCs).Utilizing a proposed supermode propagation method based on mode composition analysis,we precisely describe the operational characteristics of MSCs.Building upon this comprehensive understanding,we embark on a pioneering analysis elucidating the influence of MSC cascading order on the performance of MDMUXs.Our theoretical investigation substantiates that when constructing MDMUXs,MSCs should adhere to a specific cascading sequence.
基金National Natural Science Foundation of China(62375143,62505145)。
文摘The optical vortex interferometer(OVI)has been found to have extensive applications in precision metrology due to its ultrahigh sensitivity.However,most current implementations rely on free-space optical components,particularly requiring beam splitter cubes to combine sensing and reference beams.This inevitably necessitates complex coaxial alignment of optical paths,where tilt,divergence,and centroid misalignment introduce significant challenges in phase demodulation.This paper presents an all-fiber OVI where beams interfere through a mode selective coupler.The orbital angular momentum order difference,which directly determines the sensitivity of the OVI,is reduced to one.A novel,to our knowledge,measurement and phase demodulation methodology is developed for the optical fields generated by the proposed interferometer,accompanied by systematic analysis of error sources and their magnitudes.The proposed OVI was applied to temperature sensing,achieving a sensitivity of 44.19 rad∕(℃·m),which is 2-3 orders of magnitude higher than that of conventional methods.The proposed OVI and corresponding demodulation approach show promising potential for ultrahigh-precision sensing applications.
基金supported by the National Key Research and Development Program of China(No.2018YFB1801802)the National Natural Science Foundation of China(Nos.61835006 and 62375143)。
文摘Broadband mode converters are essential devices for space-division and wavelength-division multiplexing systems.There are great challenges in the generation of higher-order modes above the third order with low loss and high mode purity employing all-fiber devices.In this paper,an all-fiber LP_(41)mode converter is proposed and fabricated by tapering a nine-core single-mode fiber bundle.Experimental results indicate that this all-fiber LP_(41)mode converter is low-loss,high-purity,and ultrabroadband.The insertion loss is less than 0.4 dB.The purity of odd LP_(41)at 1310 nm is 95.09%,and the operating bandwidth exceeds 280 nm.
