The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy.This old approach,which was abandoned in the west in the 1940s but is generating...The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy.This old approach,which was abandoned in the west in the 1940s but is generating renewed interest,has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts.GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function.In this study,we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis.Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M.smegmatis inside the THP-1 macrophages,which may be linked to the altered lipid profile of the recombinant bacterial cell wall.In a word,this study suggested that gp30,a novel gene from a mycobacteriophage,modulated lipid composition and content to hamper the survivability of bacteria under stress conditions.展开更多
基金Key R&D and Promotion Projects of Henan Province,grant number 192102310156,the National Natural Science Foundation of China,Grant No.81601740the Applied Basic Research Program of Science&Technology Department of Sichuan Province,Grant No.2018JY0108the National Undergraduate Training Program for Innovation and Entrepreneurship(X2017013,X2019070,202010475006).
文摘The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy.This old approach,which was abandoned in the west in the 1940s but is generating renewed interest,has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts.GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function.In this study,we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis.Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M.smegmatis inside the THP-1 macrophages,which may be linked to the altered lipid profile of the recombinant bacterial cell wall.In a word,this study suggested that gp30,a novel gene from a mycobacteriophage,modulated lipid composition and content to hamper the survivability of bacteria under stress conditions.
