Most existing path planning approaches rely on discrete expansions or localized heuristics that can lead to extended re-planning,inefficient detours,and limited adaptability to complex obstacle distributions.These iss...Most existing path planning approaches rely on discrete expansions or localized heuristics that can lead to extended re-planning,inefficient detours,and limited adaptability to complex obstacle distributions.These issues are particularly pronounced when navigating cluttered or large-scale environments that demand both global coverage and smooth trajectory generation.To address these challenges,this paper proposes a Wave Water Simulator(WWS)algorithm,leveraging a physically motivated wave equation to achieve inherently smooth,globally consistent path planning.In WWS,wavefront expansions naturally identify safe corridors while seamlessly avoiding local minima,and selective corridor focusing reduces computational overhead in large or dense maps.Comprehensive simulations and real-world validations-encompassing both indoor and outdoor scenarios-demonstrate that WWS reduces path length by 2%-13%compared to conventional methods,while preserving gentle curvature and robust obstacle clearance.Furthermore,WWS requires minimal parameter tuning across diverse domains,underscoring its broad applicability to warehouse robotics,field operations,and autonomous service vehicles.These findings confirm that the proposed wave-based framework not only bridges the gap between local heuristics and global coverage but also sets a promising direction for future extensions toward dynamic obstacle scenarios and multi-agent coordination.展开更多
Drought stress orchestrates a phosphorylation-dependent signaling cascade that reprograms transcriptional networks to enhance crop resilience.Through a large-scale transgenic screening,we identified ZmCRK5A,a Ca^(2+)-...Drought stress orchestrates a phosphorylation-dependent signaling cascade that reprograms transcriptional networks to enhance crop resilience.Through a large-scale transgenic screening,we identified ZmCRK5A,a Ca^(2+)-independent calcium-dependent protein kinase(CDPK)-related kinase,as a master regulator of drought tolerance in maize.Mechanistically,ZmCRK5A directly phosphorylates the MYB transcriptional repressor ZmSMH4(Single MYB Histone 4)at three conserved serine residues(Ser42/43/59)within its SANT domain,as demonstrated by in vitro kinase assays and site-directed mutagenesis.This post-translational modification abolishes Zm SMH4's DNA-binding capacity to ACC cis-elements,thereby de-repressing the potassium influx channel gene Zm KCH1(K^(+)Channel 1).Functional validation revealed that Zm KCH1 overexpression confers drought resilience through optimized stomatal dynamics and water retention,whereas clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-generated zmkch1 mutants display hypersensitivity to water deficit.Crucially,field evaluations demonstrated preserved grain yield alongside enhanced drought tolerance in plants with activated Zm CRK5A-Zm SMH4-Zm KCH1 signaling.Our findings delineate a kinase-transcription factor-ion channel axis that dynamically fine-tunes drought responses while maintaining productivity,providing a strategic framework for engineering stress-adapted crops without yield penalties.展开更多
Developing lipid vesicles capable of self-regulation to adapt dynamically to environmental changes remains a significant challenge.In this study,we introduce a pH-responsive DNA-based transmembrane channel(p-cube)inte...Developing lipid vesicles capable of self-regulation to adapt dynamically to environmental changes remains a significant challenge.In this study,we introduce a pH-responsive DNA-based transmembrane channel(p-cube)integrated into lipid vesicles,enabling adaptive environmental responses.This nanopore,comprising four DNA strands,is anchored within lipid membranes via cholesterol modifications,with two semi-i-motif sequences strategically positioned at the apex.The i-motif structure exhibits pH-dependent folding behavior,allowing the DNA cube to reversibly open or close the nanopore in response to ambient pH variations.Fluorescence kinetic experiments demonstrated efficient,reversible modulation of transmembrane transport triggered by pH fluctuations.Furthermore,the capability of autonomous regulation under non-equilibrium conditions was validated by encapsulating glucose oxidase within vesicles and externally supplying glucose.The resulting proton generation from glucose oxidation induced pH-driven structural switching of the DNA nanopores,demonstrating environmental sensing and adaptive behavior reminiscent of natural cellular membranes.This intelligent and responsive transmembrane system holds significant potential for applications in targeted drug delivery,biosensing,and synthetic biology,providing novel approaches for precise environmental responsiveness and dynamic signaling regulation.展开更多
Background:Depression,a prevalent mental health disorder,benefits from traditional Chinese medicine(TCM).Echinacoside(ECH),a natural phenolic compound extracted from Cistanche tubulosa and Echinacea angustifolia,exhib...Background:Depression,a prevalent mental health disorder,benefits from traditional Chinese medicine(TCM).Echinacoside(ECH),a natural phenolic compound extracted from Cistanche tubulosa and Echinacea angustifolia,exhibits neuroprotective and antioxidant properties.However,research on the potential mechanism of ECH’s antidepressant activity is limited.This study explored the antidepressant potential of ECH in mice subjected to chronic unpredictable mild stress(CUMS)and its underlying molecular mechanisms.Methods:Mice received ECH(10,20,40 mg/kg/d,i.p.)during the last 14 days of a 28-day CUMS protocol.The therapeutic effect was assessed via the sucrose preference test(SPT),tail suspension test(TST)and forced swim test(FST).Hematoxylin-eosin(H&E)and Nissl staining were employed to evaluate the changes of neuronal injury in hippocampus.Network pharmacology was used to explore the potential targets and pathway enrichment in ECH-mediated antidepression.The expression changes of PI3K/AKT/Nrf2/HO-1 were evaluated by Western blotting.Furthermore,the neuroprotection effects of ECH were assessed on cultured primary neurons injured by corticosterone(CORT)using CCK-8 assay.Results:The results indicated that ECH significantly alleviated depression-like behaviors in CUMS mice characterized as the improved sucrose intake in SPT,reduced immobility duration in TST and FST,reversed weight loss and hippocampal neuronal injury induced after CUMS.PI3K/AKT was selected as core targets by network pharmacology and supported by molecular docking and dynamics simulations.WB results indicated that ECH administration offered neuroprotection by recovering the expression levels of p-PI3K,p-AKT,Nrf2,and HO-1 in CUMS mice hippocampus.Moreover,ECH rescued CORT-induced neuron death in vitro by activating PI3K/AKT/Nrf2/HO-1 pathway,which was abolished by PI3K inhibitor LY294002.Conclusion:These findings demonstrated that ECH alleviated depressive-like behaviors via PI3K/AKT/Nrf2/HO-1 activation,highlighting its potential as a novel antidepressant.展开更多
Although lead(Pb)-based perovskite solar cells(PSCs)have garnered intense attention for their remarkable photovoltaic conversion efficiency,their commercial process is urgently in need of an effective damage-evaluatio...Although lead(Pb)-based perovskite solar cells(PSCs)have garnered intense attention for their remarkable photovoltaic conversion efficiency,their commercial process is urgently in need of an effective damage-evaluation system for the early diagnosis of faulty PSCs.The main cause of microdamage in perovskite films is the outflow of Pb,which significantly impacts device performance.However,no reliable correlation has been established between classical damage detection techniques and Pb detection,resulting in limited detection sensitivity.Here,we report an in situ visual microdamage evaluation method of PSCs by coating the device surface with a silica gel encapsulation layer containing porphyrin molecules.This detection technology enables high selectivity and sensitivity based on the strong complexation between the porphyrin ring and trace Pb outflow from degraded PSCs.By establishing the linear relationship between the fluorescence intensity and Pb concentration in PSCs,trace Pb outflow is pinpointed and quantified with a low detection limit of 0.65μg cm^(-2).An applet is developed for the insitu visual fluorescence detection method to facilitate the continuous real-time monitoring of series-type PSCs,thereby enabling the prompt identification and replacement of damaged PSCs and ensuring the swift restoration of high efficiency.展开更多
We present a systematic investigation of the superconductivity in high-quality CsTi_(3)Bi_(5) single crystals by combining bulk property characterization and local-probe spectroscopy.Two successive superconducting tra...We present a systematic investigation of the superconductivity in high-quality CsTi_(3)Bi_(5) single crystals by combining bulk property characterization and local-probe spectroscopy.Two successive superconducting transitions are observed in this newly discovered kagome material.In the first stage,the diamagnetic response strengthens significantly from T_(c)~4.9 K to 4.6 K,followed by a broad transition below 4.6 K in the second stage.Moreover,different magnetic field dependences are observed for the two stages,where the first stage is field-insensitive while the second stage exhibits strong field dependence.The ultra-low magnetic field measurements indicate that the lower critical field H_(c1)(T)exhibits small anisotropy.Based on a comparative study of the superconducting state in CsBi2 and microscopic verification via scanning tunneling microscopy(STM),our results suggest the emergence of exotic and intrinsic superconductivity in this new titanium-based kagome superconductor,establishing it as a promising platform for further exploring the complexity of electronic states in the kagome lattice.展开更多
To improve the wettability of hypereutectic Al−60Si alloy and enhance the mechanical properties of the joints,Al−60Si alloy was joined by ultrasonic soldering with Sn-9Zn solder,and a sound joint with in-situ Si parti...To improve the wettability of hypereutectic Al−60Si alloy and enhance the mechanical properties of the joints,Al−60Si alloy was joined by ultrasonic soldering with Sn-9Zn solder,and a sound joint with in-situ Si particle reinforcement was obtained.The oxide film of Al−60Si alloy at the interface was identified by transmission electron microscopy(TEM)analysis as amorphous Al_(2)O_(3).The oxide of Si particles in the base metal was also alumina.The oxide film of Al−60Si alloy was observed to be removed by ultrasonic vibration instead of holding treatment.Si particle-reinforced joints(35.7 vol.%)were obtained by increasing the ultrasonication time.The maximum shear strength peaked at 99.5 MPa for soldering at 330℃with an ultrasonic vibration time of 50 s.A model of forming of Si particles reinforced joint under the ultrasound was proposed,and ultrasonic vibration was considered to promote the dissolution of Al and migration of Si particles.展开更多
Rectal neuroendocrine tumor(rNET)is an indolent malignancy often detected during colonoscopy screening.The incidence of rNET has increased approximately 10-fold over the past 30 years.Most rNETs detected during screen...Rectal neuroendocrine tumor(rNET)is an indolent malignancy often detected during colonoscopy screening.The incidence of rNET has increased approximately 10-fold over the past 30 years.Most rNETs detected during screening endoscopy are small,measuring<10 mm.Current guidelines recommend endoscopic resection for small,well-differentiated rNET using modified endoscopic submucosal resection(mEMR)or endoscopic submucosal dissection.However,the optimal endoscopic treatment method remains uncertain.This paper summarizes the evidence on mEMR with submucosal stretching,mEMR without submucosal stretching,endoscopic submucosal dissection and endoscopic full-thickness resection.Given that rNETs often exhibit submucosal invasion,achieving adequate resection depth is crucial to ensure histological complete resection.mEMR with submucosal stretching appears favorable due to its high rate of histological complete resection,safety and convenience.Risk factors associated with lymph node and distant metastases are also discussed.A treatment algorithm is proposed to facilitate clinical decision-making.展开更多
The stability of perovskite solar cells(PSCs)is adversely affected by nonradiative recombination resulting from buried interface defects.Herein,we synthesize a polyionic liquid,poly(p-vinylbenzyl trimethylam-monium he...The stability of perovskite solar cells(PSCs)is adversely affected by nonradiative recombination resulting from buried interface defects.Herein,we synthesize a polyionic liquid,poly(p-vinylbenzyl trimethylam-monium hexafluorophosphate)(PTA),and introduce it into the buried interface of PSCs.The quaternary ammonium cation(N(-CH_(3))^(3+))in PTA can fill the vacancies of organic cations within the perovskite structure and reduce shallow energy level defects.Additionally,the hexafluorophosphate(PF6−)in PTA forms a Lewis acid-base interaction with Pb^(2+)in the perovskite layer,effectively passivating deep en-ergy level defects.Furthermore,hydrogen bonding can be established between organic cations and the PF6−anion,preventing the formation of shallow energy level defects.Through this synergistic mecha-nism,the deep and shallow energy level defects are effectively mitigated,resulting in improved device performance.As a result,the resulting treated inverted PSC exhibits an impressive power conversion ef-ficiency(PCE)of 24.72%.Notably,the PTA-treated PSCs exhibit remarkable stability,with 88.5%of the original PCE retained after undergoing heat aging at 85℃ for 1078 h,and 89.1%of the initial PCE main-tained following continuous exposure to light for 1100 h at the maximum power point.Synergistically suppressing multiple defects at the buried interface through the use of polyionic liquids is a promising way to improve the commercial viability of PSCs.展开更多
Refractory high-entropy alloys(RHEAs)are promising for high-temperature applications due to their ex-ceptional mechanical properties at high temperatures.However,limited studies on their high-temperature fatigue behav...Refractory high-entropy alloys(RHEAs)are promising for high-temperature applications due to their ex-ceptional mechanical properties at high temperatures.However,limited studies on their high-temperature fatigue behavior hinder further development.This study systematically investigates the low-cycle fatigue(LCF)behavior of HfNbTiZr RHEA at room temperature(25℃)and elevated temperatures(350,450,and 600℃)through a combination of experimental analyses and dislocation-based damage-coupled crystal plasticity finite element(CPFE)simulations,to unveil the effects of creep damage on LCF behavior at varying temperatures.The results indicate that the LCF life dramatically decreases at an increased tem-perature,shifting from transgranular fatigue damage at lower temperatures(25-350℃)to a dual damage mechanism involving both intergranular fatigue and creep damage at higher temperatures(450-600℃).At 600℃,creep damage notably contributes to the accumulation of geometrically necessary dislocations(GNDs),crack initiation,and propagation at grain boundaries,and thus accelerates LCF failure.Compara-tive CPFE simulations reveal that creep damage significantly contributes to cyclic softening and reduction in elastic modulus,which also amplifies the strain localization under the LCF loading.The contribution of creep damage to the total stored energy density(SED)representing the overall damage increases with temperatures,accounting for 11%at 600℃.Additionally,CPFE simulations indicate that the creep dam-age notably influences the magnitude of GND density localized at grain boundaries.This study provides critical insights into the fatigue damage mechanisms of RHEAs,offering valuable guidance for their ap-plication in high temperatures.展开更多
Objective This study aimed to analyse the trend of the mental disorder spectrum in children and adolescents from 2014 to 2022 in one city in Central China and to provide actionable recommendations for the prevention a...Objective This study aimed to analyse the trend of the mental disorder spectrum in children and adolescents from 2014 to 2022 in one city in Central China and to provide actionable recommendations for the prevention and management of mental disorders.Methods In this hospital-based retrospective study,we utilized child and adolescent medical records data from the Wuhan Mental Health Center from January 2014 to December 2022 and examined the top 5 mental disorders(schizophrenia,depressive episode,bipolar disorder,pervasive developmental disorder,and unspecified mood disorder)that accounted for the overall proportion of patients admitted.The rank and proportion of these mental disorders,demographic characteristics and disease indicators were analysed.Results There was a significant upwards trend in the number of children and adolescents diagnosed with mental disorders over the past 9 years,with a sharp decline in 2020 due to the COVID-19 pandemic,followed by a rebound in 2021 and a sustained level above prepandemic figures by 2022.The average age of hospitalization decreased significantly from 20.7 to 16.2 years,with a marked increase in the 12-17-year-old age group.The proportion of female hospitalizations increased from 39.2%to 55.2%,with a corresponding decrease in male hospitalizations.There was a notable decrease in the proportion of schizophrenia cases and an ascent of depressive episode to the most prevalent position.Conclusion This study emphasizes the critical need for targeted interventions and resources for severe mental disorders in children and adolescents and the importance of early detection and management of mental disorders to mitigate long-term effects on well-being and development.展开更多
A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface supercond...A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface superconducting gaps and topological surface states have been observed.However,the simultaneous presence of bulk and surface superconductivity has remained unresolved.In this study,we directly visualize coexisting bulk and surface superconducting gaps in trigonal PtBi_(2) using ultra-low-temperature scanning tunneling microscopy/spectroscopy.The bulk gap,Δ,is∼0.053 meV,with a critical temperature(T_(c))of∼0.5K and a critical field below 0.01 T,accompanied by a vortex lattice and vortex bound states and yielding a coherence length of∼200 nm.Remarkably,certain surface regions exhibit a much larger gap(Δ)of∼0.42 meV,persisting up to a T_(c)value of∼3K and surviving magnetic fields of up to 2 T,yet lacking a static vortex lattice.This coexistence of robust surface and bulk superconductivity establishes γ-PtBi_(2)as a unique platform for exploring the interplay between bulk and surface Cooper pairings in topological superconductors.展开更多
Recent advances in strain engineering have enabled unprecedented control over quantum states in strongly correlated magnetic systems.However,nanoscale strain modulation of charge density waves(CDWs)and magnetically ex...Recent advances in strain engineering have enabled unprecedented control over quantum states in strongly correlated magnetic systems.However,nanoscale strain modulation of charge density waves(CDWs)and magnetically excited states,which is crucial for atomically precise strain engineering and practical spintronic applications,remains unexplored.Here,we report the nanoscale strain effects on CDWs and low-energy electronic states in the van der Waals antiferromagnetic metal GdTe_(3),utilizing scanning tunneling microscopy/spectroscopy.Lowtemperature cleavage introduces local strains,resulting in the formation of nanoscale wrinkles on the GdTe_(3)surface.Atomic displacement analysis reveals two distinct types of wrinkles:Wrinkle-I,originating from unidirectional strain,and Wrinkle-II,dominated by shear strain.In Wrinkle-I,the tensile strain enhances the CDW gap,while the compressive strain induces a single low-energy magnetic state.Wrinkle-II switches the orientation of CDW,leading to the formation of an associated CDW domain wall.In addition,three low-energy magnetic states that exhibit magnetic field-dependent shifts and intensity variations emerge within the CDW gap around Wrinkle-II,indicative of a strain-tuned coupling between CDW order and localized 4f-electron magnetism.These findings establish nanoscale strain as a powerful tuning knob for manipulating intertwined electronic and magnetic excitations in correlated magnetic systems.展开更多
Influenza,an acute respiratory infectious disease caused by the influenza virus,exhibits distinct seasonal patterns in China,with peak activity occurring in winter and spring in northern regions,and in winter and summ...Influenza,an acute respiratory infectious disease caused by the influenza virus,exhibits distinct seasonal patterns in China,with peak activity occurring in winter and spring in northern regions,and in winter and summer in southern areas[1].The World Health Organization(WHO)emphasizes that early warning and epidemic intensity assessments are critical public health strategies for influenza prevention and control.Internet-based flu surveillance,with real-time data and low costs,effectively complements traditional methods.The Baidu Search Index,which reflects flu-related queries,strongly correlates with influenza trends,aiding in regional activity assessment and outbreak tracking[2].展开更多
A gradient coating containing collagen and inorganic strontium/calcium phosphate(Sr/CaP)was fabricated on plasma-electrolytically oxidised magnesium via one-step cathodic electrodeposition.First,Sr-doped dicalcium pho...A gradient coating containing collagen and inorganic strontium/calcium phosphate(Sr/CaP)was fabricated on plasma-electrolytically oxidised magnesium via one-step cathodic electrodeposition.First,Sr-doped dicalcium phosphate dihydrate and hydroxyapatite(DCPD and HA)was deposited,followed by a collagen/CaP layer.The morphological evolution,sequential degradation behaviour,and in vitro bio-properties of the coatings were investigated.The incorporation of collagen remarkably refined the morphology of the CaP,and a more aggregated nano-spherical morphology was observed with increasing collagen concentration.Sr could partially replace Ca in the CaP crystals.Collagen combined with CaP formed a relatively stable skeletal frame,which provided sufficient barrier properties and more sites for the re-precipitation of bone tissue,as well as a more promising proliferation and differentiation ability of osteoblasts.A gradient coating that matches the requirements of bone growth at various periods is suggested for implantation.展开更多
Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been show...Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been shown to promote rumen epithelial growth in several sheep trials.Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture,along with the associated molecular mechanisms,remain unknown.We used 2045-day-old weaned lambs to investigate the specific proteins and molecular mechanisms involved in these processes.Half of the lambs were fed yeast culture,and the other half were used as controls.Results Yeast culture enhanced growth performance,facilitated rumen fermentation,and promoted rumen papilla development in weaned lambs.Proteomics data identified 4,831 proteins in the rumen epithelial tissue of lambs,comprising 87 upregulated and 425 downregulated proteins.Administration of yeast culture activated multiple molecular functions within rumen epithelial cells,including oxidative phosphorylation,glutathione metabolism,apoptosis,cell cycle,and vitamin digestion and absorption.The expression of proteins associated with cell cycle regulation increased,whereas those associated with apoptosis decreased.Administration of yeast culture also reduced the duration of the G0/G1 phase of rumen epithelial cells and accelerated the cell cycle.Furthermore,yeast culture showed increased cyclin D1,cyclin-dependent kinase(CDK)2,CDK4,CDK6,and cyclin E1 expressions and decreased cytochrome C(Cyto-c),Bcl-2-related X protein(Bax),cleaved caspase 3(C-caspase 3),caspase 3,and cleaved caspase 7(C-caspase 7)protein expressions.Yeast culture upregulated the insulin-like growth factor-1 receptor(IGF-1R)and insulin-like growth factor-binding protein 5(IGFBP-5)mRNA expressions in rumen epithelial cells.Conclusions Yeast culture facilitates rumen epithelial development by regulating the cell cycle and IGF-1 signaling and reducing the expression of proteins associated with apoptosis in rumen epithelial cells.The findings of this study provide novel insights into the molecular mechanisms through which yeast culture promotes rumen epithelial development in weaned lambs.展开更多
文摘Most existing path planning approaches rely on discrete expansions or localized heuristics that can lead to extended re-planning,inefficient detours,and limited adaptability to complex obstacle distributions.These issues are particularly pronounced when navigating cluttered or large-scale environments that demand both global coverage and smooth trajectory generation.To address these challenges,this paper proposes a Wave Water Simulator(WWS)algorithm,leveraging a physically motivated wave equation to achieve inherently smooth,globally consistent path planning.In WWS,wavefront expansions naturally identify safe corridors while seamlessly avoiding local minima,and selective corridor focusing reduces computational overhead in large or dense maps.Comprehensive simulations and real-world validations-encompassing both indoor and outdoor scenarios-demonstrate that WWS reduces path length by 2%-13%compared to conventional methods,while preserving gentle curvature and robust obstacle clearance.Furthermore,WWS requires minimal parameter tuning across diverse domains,underscoring its broad applicability to warehouse robotics,field operations,and autonomous service vehicles.These findings confirm that the proposed wave-based framework not only bridges the gap between local heuristics and global coverage but also sets a promising direction for future extensions toward dynamic obstacle scenarios and multi-agent coordination.
基金supported by National Key Research and Development Program of China(2022YFF1001600)BeijingNatural Science Foundation(5244040)+1 种基金STI2030-Major Projects(2030ZD0407101)China Postdoctoral Science Foundation(2022M723435)。
文摘Drought stress orchestrates a phosphorylation-dependent signaling cascade that reprograms transcriptional networks to enhance crop resilience.Through a large-scale transgenic screening,we identified ZmCRK5A,a Ca^(2+)-independent calcium-dependent protein kinase(CDPK)-related kinase,as a master regulator of drought tolerance in maize.Mechanistically,ZmCRK5A directly phosphorylates the MYB transcriptional repressor ZmSMH4(Single MYB Histone 4)at three conserved serine residues(Ser42/43/59)within its SANT domain,as demonstrated by in vitro kinase assays and site-directed mutagenesis.This post-translational modification abolishes Zm SMH4's DNA-binding capacity to ACC cis-elements,thereby de-repressing the potassium influx channel gene Zm KCH1(K^(+)Channel 1).Functional validation revealed that Zm KCH1 overexpression confers drought resilience through optimized stomatal dynamics and water retention,whereas clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-generated zmkch1 mutants display hypersensitivity to water deficit.Crucially,field evaluations demonstrated preserved grain yield alongside enhanced drought tolerance in plants with activated Zm CRK5A-Zm SMH4-Zm KCH1 signaling.Our findings delineate a kinase-transcription factor-ion channel axis that dynamically fine-tunes drought responses while maintaining productivity,providing a strategic framework for engineering stress-adapted crops without yield penalties.
基金supported by the National Natural Science Foundation of China(22377025,22177032,22307035,22474037)the Postdoctoral Science Foundation of China(2024M761361)the Natural Science Foundation in Hunan Province(2023JJ40135,2022JJ20038)。
文摘Developing lipid vesicles capable of self-regulation to adapt dynamically to environmental changes remains a significant challenge.In this study,we introduce a pH-responsive DNA-based transmembrane channel(p-cube)integrated into lipid vesicles,enabling adaptive environmental responses.This nanopore,comprising four DNA strands,is anchored within lipid membranes via cholesterol modifications,with two semi-i-motif sequences strategically positioned at the apex.The i-motif structure exhibits pH-dependent folding behavior,allowing the DNA cube to reversibly open or close the nanopore in response to ambient pH variations.Fluorescence kinetic experiments demonstrated efficient,reversible modulation of transmembrane transport triggered by pH fluctuations.Furthermore,the capability of autonomous regulation under non-equilibrium conditions was validated by encapsulating glucose oxidase within vesicles and externally supplying glucose.The resulting proton generation from glucose oxidation induced pH-driven structural switching of the DNA nanopores,demonstrating environmental sensing and adaptive behavior reminiscent of natural cellular membranes.This intelligent and responsive transmembrane system holds significant potential for applications in targeted drug delivery,biosensing,and synthetic biology,providing novel approaches for precise environmental responsiveness and dynamic signaling regulation.
基金funded by the Clinical Research Project(No.2024LC2432)the National Natural Science Foundation of China(No.82071515)the Key Research and Development Program of Shaanxi Province(No.2024SF-YBXM-061).
文摘Background:Depression,a prevalent mental health disorder,benefits from traditional Chinese medicine(TCM).Echinacoside(ECH),a natural phenolic compound extracted from Cistanche tubulosa and Echinacea angustifolia,exhibits neuroprotective and antioxidant properties.However,research on the potential mechanism of ECH’s antidepressant activity is limited.This study explored the antidepressant potential of ECH in mice subjected to chronic unpredictable mild stress(CUMS)and its underlying molecular mechanisms.Methods:Mice received ECH(10,20,40 mg/kg/d,i.p.)during the last 14 days of a 28-day CUMS protocol.The therapeutic effect was assessed via the sucrose preference test(SPT),tail suspension test(TST)and forced swim test(FST).Hematoxylin-eosin(H&E)and Nissl staining were employed to evaluate the changes of neuronal injury in hippocampus.Network pharmacology was used to explore the potential targets and pathway enrichment in ECH-mediated antidepression.The expression changes of PI3K/AKT/Nrf2/HO-1 were evaluated by Western blotting.Furthermore,the neuroprotection effects of ECH were assessed on cultured primary neurons injured by corticosterone(CORT)using CCK-8 assay.Results:The results indicated that ECH significantly alleviated depression-like behaviors in CUMS mice characterized as the improved sucrose intake in SPT,reduced immobility duration in TST and FST,reversed weight loss and hippocampal neuronal injury induced after CUMS.PI3K/AKT was selected as core targets by network pharmacology and supported by molecular docking and dynamics simulations.WB results indicated that ECH administration offered neuroprotection by recovering the expression levels of p-PI3K,p-AKT,Nrf2,and HO-1 in CUMS mice hippocampus.Moreover,ECH rescued CORT-induced neuron death in vitro by activating PI3K/AKT/Nrf2/HO-1 pathway,which was abolished by PI3K inhibitor LY294002.Conclusion:These findings demonstrated that ECH alleviated depressive-like behaviors via PI3K/AKT/Nrf2/HO-1 activation,highlighting its potential as a novel antidepressant.
基金financial support from the Shccig-Qinling Program(SMYJY202300294C)the Science,Technology,and Innovation Commission of Shenzhen Municipality(GJHZ20220913143204008,JCYJ20220818103417036)+1 种基金the National Natural Science Foundation of China(22261142666,52172237,52372225)the Shaanxi Science Fund for Distinguished Young Scholars(2022JC-21)。
文摘Although lead(Pb)-based perovskite solar cells(PSCs)have garnered intense attention for their remarkable photovoltaic conversion efficiency,their commercial process is urgently in need of an effective damage-evaluation system for the early diagnosis of faulty PSCs.The main cause of microdamage in perovskite films is the outflow of Pb,which significantly impacts device performance.However,no reliable correlation has been established between classical damage detection techniques and Pb detection,resulting in limited detection sensitivity.Here,we report an in situ visual microdamage evaluation method of PSCs by coating the device surface with a silica gel encapsulation layer containing porphyrin molecules.This detection technology enables high selectivity and sensitivity based on the strong complexation between the porphyrin ring and trace Pb outflow from degraded PSCs.By establishing the linear relationship between the fluorescence intensity and Pb concentration in PSCs,trace Pb outflow is pinpointed and quantified with a low detection limit of 0.65μg cm^(-2).An applet is developed for the insitu visual fluorescence detection method to facilitate the continuous real-time monitoring of series-type PSCs,thereby enabling the prompt identification and replacement of damaged PSCs and ensuring the swift restoration of high efficiency.
基金supported by the National Key Research and Development Program of China(Grant Nos.2024YFA1611102,2022YFA1403903,2023YFA1406101,and 2022YFA1204100)the National Natural Science Foundation of China(Grant Nos.12304075 and 62488201)+1 种基金CAS Project for Young Scientists in Basic Research(Grant Nos.2022YSBR-048 and YSBR-003)the Innovation Program of Quantum Science and Technology(Grant No.2021ZD0302700)。
文摘We present a systematic investigation of the superconductivity in high-quality CsTi_(3)Bi_(5) single crystals by combining bulk property characterization and local-probe spectroscopy.Two successive superconducting transitions are observed in this newly discovered kagome material.In the first stage,the diamagnetic response strengthens significantly from T_(c)~4.9 K to 4.6 K,followed by a broad transition below 4.6 K in the second stage.Moreover,different magnetic field dependences are observed for the two stages,where the first stage is field-insensitive while the second stage exhibits strong field dependence.The ultra-low magnetic field measurements indicate that the lower critical field H_(c1)(T)exhibits small anisotropy.Based on a comparative study of the superconducting state in CsBi2 and microscopic verification via scanning tunneling microscopy(STM),our results suggest the emergence of exotic and intrinsic superconductivity in this new titanium-based kagome superconductor,establishing it as a promising platform for further exploring the complexity of electronic states in the kagome lattice.
基金financial support from the National Natural Science Foundation of China(Nos.52275385,U2167216)Sichuan Province Science and Technology Support Program,China(No.2022YFG0086).
文摘To improve the wettability of hypereutectic Al−60Si alloy and enhance the mechanical properties of the joints,Al−60Si alloy was joined by ultrasonic soldering with Sn-9Zn solder,and a sound joint with in-situ Si particle reinforcement was obtained.The oxide film of Al−60Si alloy at the interface was identified by transmission electron microscopy(TEM)analysis as amorphous Al_(2)O_(3).The oxide of Si particles in the base metal was also alumina.The oxide film of Al−60Si alloy was observed to be removed by ultrasonic vibration instead of holding treatment.Si particle-reinforced joints(35.7 vol.%)were obtained by increasing the ultrasonication time.The maximum shear strength peaked at 99.5 MPa for soldering at 330℃with an ultrasonic vibration time of 50 s.A model of forming of Si particles reinforced joint under the ultrasound was proposed,and ultrasonic vibration was considered to promote the dissolution of Al and migration of Si particles.
基金Supported by Nanchang High-Level Scientific and Technological Innovation Talents‘Double Hundred Plan’Project,China,No.2022-312.
文摘Rectal neuroendocrine tumor(rNET)is an indolent malignancy often detected during colonoscopy screening.The incidence of rNET has increased approximately 10-fold over the past 30 years.Most rNETs detected during screening endoscopy are small,measuring<10 mm.Current guidelines recommend endoscopic resection for small,well-differentiated rNET using modified endoscopic submucosal resection(mEMR)or endoscopic submucosal dissection.However,the optimal endoscopic treatment method remains uncertain.This paper summarizes the evidence on mEMR with submucosal stretching,mEMR without submucosal stretching,endoscopic submucosal dissection and endoscopic full-thickness resection.Given that rNETs often exhibit submucosal invasion,achieving adequate resection depth is crucial to ensure histological complete resection.mEMR with submucosal stretching appears favorable due to its high rate of histological complete resection,safety and convenience.Risk factors associated with lymph node and distant metastases are also discussed.A treatment algorithm is proposed to facilitate clinical decision-making.
基金supported by the Science,Technology,and Innovation Commission of Shenzhen Municipality(No.GJHZ20220913143204008)the Shccig-Qinling Program(No.SMYJY202300294C)+3 种基金National Natural Science Foundation of China(Nos.22261142666,52372225,52172237,22305191)the Shaanxi Science Fund for Distinguished Young Scholars(No.2022JC-21)the Research Fund of the State Key Laboratory of Solidification Processing(NPU)China(No.2021-QZ-02).
文摘The stability of perovskite solar cells(PSCs)is adversely affected by nonradiative recombination resulting from buried interface defects.Herein,we synthesize a polyionic liquid,poly(p-vinylbenzyl trimethylam-monium hexafluorophosphate)(PTA),and introduce it into the buried interface of PSCs.The quaternary ammonium cation(N(-CH_(3))^(3+))in PTA can fill the vacancies of organic cations within the perovskite structure and reduce shallow energy level defects.Additionally,the hexafluorophosphate(PF6−)in PTA forms a Lewis acid-base interaction with Pb^(2+)in the perovskite layer,effectively passivating deep en-ergy level defects.Furthermore,hydrogen bonding can be established between organic cations and the PF6−anion,preventing the formation of shallow energy level defects.Through this synergistic mecha-nism,the deep and shallow energy level defects are effectively mitigated,resulting in improved device performance.As a result,the resulting treated inverted PSC exhibits an impressive power conversion ef-ficiency(PCE)of 24.72%.Notably,the PTA-treated PSCs exhibit remarkable stability,with 88.5%of the original PCE retained after undergoing heat aging at 85℃ for 1078 h,and 89.1%of the initial PCE main-tained following continuous exposure to light for 1100 h at the maximum power point.Synergistically suppressing multiple defects at the buried interface through the use of polyionic liquids is a promising way to improve the commercial viability of PSCs.
基金National Science Foundation of China(Nos.52401212 and52401214)the National Science Foundation of Jiangsu Province(No.BK20241020)+1 种基金the Avi-ation Foundation(No.2023Z0530S6004)the Jiangsu Province University Collaborative Innovation Centre(High-Tech Ships)Pro-gram(No.XTCX202401).
文摘Refractory high-entropy alloys(RHEAs)are promising for high-temperature applications due to their ex-ceptional mechanical properties at high temperatures.However,limited studies on their high-temperature fatigue behavior hinder further development.This study systematically investigates the low-cycle fatigue(LCF)behavior of HfNbTiZr RHEA at room temperature(25℃)and elevated temperatures(350,450,and 600℃)through a combination of experimental analyses and dislocation-based damage-coupled crystal plasticity finite element(CPFE)simulations,to unveil the effects of creep damage on LCF behavior at varying temperatures.The results indicate that the LCF life dramatically decreases at an increased tem-perature,shifting from transgranular fatigue damage at lower temperatures(25-350℃)to a dual damage mechanism involving both intergranular fatigue and creep damage at higher temperatures(450-600℃).At 600℃,creep damage notably contributes to the accumulation of geometrically necessary dislocations(GNDs),crack initiation,and propagation at grain boundaries,and thus accelerates LCF failure.Compara-tive CPFE simulations reveal that creep damage significantly contributes to cyclic softening and reduction in elastic modulus,which also amplifies the strain localization under the LCF loading.The contribution of creep damage to the total stored energy density(SED)representing the overall damage increases with temperatures,accounting for 11%at 600℃.Additionally,CPFE simulations indicate that the creep dam-age notably influences the magnitude of GND density localized at grain boundaries.This study provides critical insights into the fatigue damage mechanisms of RHEAs,offering valuable guidance for their ap-plication in high temperatures.
文摘Objective This study aimed to analyse the trend of the mental disorder spectrum in children and adolescents from 2014 to 2022 in one city in Central China and to provide actionable recommendations for the prevention and management of mental disorders.Methods In this hospital-based retrospective study,we utilized child and adolescent medical records data from the Wuhan Mental Health Center from January 2014 to December 2022 and examined the top 5 mental disorders(schizophrenia,depressive episode,bipolar disorder,pervasive developmental disorder,and unspecified mood disorder)that accounted for the overall proportion of patients admitted.The rank and proportion of these mental disorders,demographic characteristics and disease indicators were analysed.Results There was a significant upwards trend in the number of children and adolescents diagnosed with mental disorders over the past 9 years,with a sharp decline in 2020 due to the COVID-19 pandemic,followed by a rebound in 2021 and a sustained level above prepandemic figures by 2022.The average age of hospitalization decreased significantly from 20.7 to 16.2 years,with a marked increase in the 12-17-year-old age group.The proportion of female hospitalizations increased from 39.2%to 55.2%,with a corresponding decrease in male hospitalizations.There was a notable decrease in the proportion of schizophrenia cases and an ascent of depressive episode to the most prevalent position.Conclusion This study emphasizes the critical need for targeted interventions and resources for severe mental disorders in children and adolescents and the importance of early detection and management of mental disorders to mitigate long-term effects on well-being and development.
基金supported by the National Natural Science Foundation of China(Grant No.62488201)the National Key Research and Development Projects of China(Grant Nos.2022YFA1204100 and 2023YFA1607400)+1 种基金the CAS Project for Young Scientists in Basic Research(Grant No.YSBR-003)the Innovation Program of Quantum Science and Technology(Grant No.2021ZD0302700)。
文摘A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface superconducting gaps and topological surface states have been observed.However,the simultaneous presence of bulk and surface superconductivity has remained unresolved.In this study,we directly visualize coexisting bulk and surface superconducting gaps in trigonal PtBi_(2) using ultra-low-temperature scanning tunneling microscopy/spectroscopy.The bulk gap,Δ,is∼0.053 meV,with a critical temperature(T_(c))of∼0.5K and a critical field below 0.01 T,accompanied by a vortex lattice and vortex bound states and yielding a coherence length of∼200 nm.Remarkably,certain surface regions exhibit a much larger gap(Δ)of∼0.42 meV,persisting up to a T_(c)value of∼3K and surviving magnetic fields of up to 2 T,yet lacking a static vortex lattice.This coexistence of robust surface and bulk superconductivity establishes γ-PtBi_(2)as a unique platform for exploring the interplay between bulk and surface Cooper pairings in topological superconductors.
基金supported by the National Natural Science Foundation of China(Grant No.62488201)the National Key Research and Development Project of China(Grant No.2022YFA1204100)+1 种基金the Chinese Academy of Sciences Project for Young Scientists in Basic Research(Grant No.YSBR-003)the Innovation Program of Quantum Science and Technology(Grant No.2021ZD0302700).
文摘Recent advances in strain engineering have enabled unprecedented control over quantum states in strongly correlated magnetic systems.However,nanoscale strain modulation of charge density waves(CDWs)and magnetically excited states,which is crucial for atomically precise strain engineering and practical spintronic applications,remains unexplored.Here,we report the nanoscale strain effects on CDWs and low-energy electronic states in the van der Waals antiferromagnetic metal GdTe_(3),utilizing scanning tunneling microscopy/spectroscopy.Lowtemperature cleavage introduces local strains,resulting in the formation of nanoscale wrinkles on the GdTe_(3)surface.Atomic displacement analysis reveals two distinct types of wrinkles:Wrinkle-I,originating from unidirectional strain,and Wrinkle-II,dominated by shear strain.In Wrinkle-I,the tensile strain enhances the CDW gap,while the compressive strain induces a single low-energy magnetic state.Wrinkle-II switches the orientation of CDW,leading to the formation of an associated CDW domain wall.In addition,three low-energy magnetic states that exhibit magnetic field-dependent shifts and intensity variations emerge within the CDW gap around Wrinkle-II,indicative of a strain-tuned coupling between CDW order and localized 4f-electron magnetism.These findings establish nanoscale strain as a powerful tuning knob for manipulating intertwined electronic and magnetic excitations in correlated magnetic systems.
基金supported by the National Key Research and Development Program of China(Project No.2023YFC2307500).
文摘Influenza,an acute respiratory infectious disease caused by the influenza virus,exhibits distinct seasonal patterns in China,with peak activity occurring in winter and spring in northern regions,and in winter and summer in southern areas[1].The World Health Organization(WHO)emphasizes that early warning and epidemic intensity assessments are critical public health strategies for influenza prevention and control.Internet-based flu surveillance,with real-time data and low costs,effectively complements traditional methods.The Baidu Search Index,which reflects flu-related queries,strongly correlates with influenza trends,aiding in regional activity assessment and outbreak tracking[2].
基金support from Mobility Programme of the Sino-German Center(M-0056)National Natural Science Foundation of China(52101286)+2 种基金Natural Science Foundation of Liaoning Province(2022-YGJC-16)Fundamental Research Funds for the Central Universities(N2302017)Supported by Sichuan Science and Technology Program 2023ZYD0115Shenyang Young and Middle-aged Science and Technology Innovation Talent Support Program(RC231178).
文摘A gradient coating containing collagen and inorganic strontium/calcium phosphate(Sr/CaP)was fabricated on plasma-electrolytically oxidised magnesium via one-step cathodic electrodeposition.First,Sr-doped dicalcium phosphate dihydrate and hydroxyapatite(DCPD and HA)was deposited,followed by a collagen/CaP layer.The morphological evolution,sequential degradation behaviour,and in vitro bio-properties of the coatings were investigated.The incorporation of collagen remarkably refined the morphology of the CaP,and a more aggregated nano-spherical morphology was observed with increasing collagen concentration.Sr could partially replace Ca in the CaP crystals.Collagen combined with CaP formed a relatively stable skeletal frame,which provided sufficient barrier properties and more sites for the re-precipitation of bone tissue,as well as a more promising proliferation and differentiation ability of osteoblasts.A gradient coating that matches the requirements of bone growth at various periods is suggested for implantation.
基金supported by the National Key Research and Development Program of China(2023YFE0100400)Science and Technology Project of Inner Mongolia Autonomous Region(2020GG0036)+2 种基金Basic Scientific Research Business Project of Universities directly under the Inner Mongolia Autonomous Region(BR22-11-17)National Center of Technology Innovation for Dairy(2023-JSGG-5)the Special Project for Improving the Research Ability of Young Teachers of Inner Mongolia Agricultural University(BR220133).
文摘Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been shown to promote rumen epithelial growth in several sheep trials.Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture,along with the associated molecular mechanisms,remain unknown.We used 2045-day-old weaned lambs to investigate the specific proteins and molecular mechanisms involved in these processes.Half of the lambs were fed yeast culture,and the other half were used as controls.Results Yeast culture enhanced growth performance,facilitated rumen fermentation,and promoted rumen papilla development in weaned lambs.Proteomics data identified 4,831 proteins in the rumen epithelial tissue of lambs,comprising 87 upregulated and 425 downregulated proteins.Administration of yeast culture activated multiple molecular functions within rumen epithelial cells,including oxidative phosphorylation,glutathione metabolism,apoptosis,cell cycle,and vitamin digestion and absorption.The expression of proteins associated with cell cycle regulation increased,whereas those associated with apoptosis decreased.Administration of yeast culture also reduced the duration of the G0/G1 phase of rumen epithelial cells and accelerated the cell cycle.Furthermore,yeast culture showed increased cyclin D1,cyclin-dependent kinase(CDK)2,CDK4,CDK6,and cyclin E1 expressions and decreased cytochrome C(Cyto-c),Bcl-2-related X protein(Bax),cleaved caspase 3(C-caspase 3),caspase 3,and cleaved caspase 7(C-caspase 7)protein expressions.Yeast culture upregulated the insulin-like growth factor-1 receptor(IGF-1R)and insulin-like growth factor-binding protein 5(IGFBP-5)mRNA expressions in rumen epithelial cells.Conclusions Yeast culture facilitates rumen epithelial development by regulating the cell cycle and IGF-1 signaling and reducing the expression of proteins associated with apoptosis in rumen epithelial cells.The findings of this study provide novel insights into the molecular mechanisms through which yeast culture promotes rumen epithelial development in weaned lambs.
