A carbonic anhydrase( CA) transcript was obtained from the Contig library according to the published sequencing information of the buckwheat transcripts. The full length of the CA gene was amplified by reverse transcr...A carbonic anhydrase( CA) transcript was obtained from the Contig library according to the published sequencing information of the buckwheat transcripts. The full length of the CA gene was amplified by reverse transcription PCR( RT-PCR). The bioinformatics analysis showed that the full length of Fs CA1 gene was 1233 bp and open reading frame was 978 bp,and encoding 325 amino acids. The molecular weight was 35. 11 ku and the isoelectric point was 7. 59; there were 9 α helices,6 β folds,many randon coil and extension chain,containing one signal peptide and one transmembrane region,having a 2 amino acid conserved domains with typical beta-type carbonic anhydrase. Subcellular localization showed that the protein is most likely to appear in the chloroplast. The three-dimensional structure model of Fs CA1 was built by homologous modeling method,indicating that the homo-octamer of buckwheat CA and pea CA could match well,so it can be inferred that buckwheat CA is also homo-octamer. Real-time quantitative PCR was used to detect the expression of Fs CA1 in different organs of buckwheat.The results showed that Fs CA1 had the highest expression level in leaves,then in the stems,and the lowest in roots.展开更多
Objective: to investigate the application effect of mental rehabilitation nursing in long-term hospitalization of elderly patients with chronic schizophrenia. Methods: a total of 62 long-term hospitalized elderly pati...Objective: to investigate the application effect of mental rehabilitation nursing in long-term hospitalization of elderly patients with chronic schizophrenia. Methods: a total of 62 long-term hospitalized elderly patients with chronic schizophrenia were divided into 31 cases in groups A and B by random number table method, and the study was carried out from January 2021 to December 2021. The study gave all patients routine nursing interventions, and group B added psychiatric rehabilitation nursing interventions and compared the effects of the interventions. Results: the IRROS and PSQI scale scores in group B were both lower than those in group A, the scores on SQLS and ADL scales were higher than those in group A, and the satisfaction of nursing care was also higher than that in group A, and the above comparisons were statistically significant (P〈0.05). Conclusion: the application of psychiatric rehabilitation nursing can significantly improve the rehabilitation effect of elderly patients with chronic schizophrenia, and actively improve the quality of sleep, as well as the ability to live in daily life and the quality of life.展开更多
Grassroots power supply enterprises are at the forefront of providing customers with quality services, closely related to the interests of the people, and effectively do a good job of management within the scope of th...Grassroots power supply enterprises are at the forefront of providing customers with quality services, closely related to the interests of the people, and effectively do a good job of management within the scope of the team, so that the enterprise construction and team staff construction on the same line, conducive to the long-term development of the enterprise. The basic-level team is the basic part of power supply enterprise, is the foundation of the enterprise, thus promote team building mechanism can make the management more efficient, grass-roots team to be more perfect, to enriching the connotation of the team, strengthen the team construction, improve the core competitiveness of enterprises, the development of grassroots power supply enterprise has the vital role.展开更多
With rapid development of femtocell, dense deployment of femtocells in buildings will become an important study scenario. In this scenario, there exists severe inter-femtocell interference in the same building, which ...With rapid development of femtocell, dense deployment of femtocells in buildings will become an important study scenario. In this scenario, there exists severe inter-femtocell interference in the same building, which is revealed by our simulations in the 3-dimention （3D） scenario. If this type of interference is not well controlled, services to indoor users are bound to be deteriorated, especially for the femtocell edge users. Motivated by this problem, femto users＇ received interference model is constructed based on the scenario of 3D femtocell deployment. Then, a graph theory based in-building inter-femtocell coordination scheme is proposed, which includes three phases： establishment of femtocell interference graph, femtocell clustering and frequency resources allocation based on the proposed cluster influence circle. Finally, the simulation results confirm that the proposed scheme improves the femtocell average throughput by 22.4% and 26.2% in comparison with frequency universal reuse scheme and channel demodulation reliability of cell edge femto users. frequency hopping scheme respectively, and ensures the展开更多
基金Supported by Project of National Natural Science Foundation(31360300&31560362)Key Project of the Tibet Autonomous Region(XZXTCX-2016)
文摘A carbonic anhydrase( CA) transcript was obtained from the Contig library according to the published sequencing information of the buckwheat transcripts. The full length of the CA gene was amplified by reverse transcription PCR( RT-PCR). The bioinformatics analysis showed that the full length of Fs CA1 gene was 1233 bp and open reading frame was 978 bp,and encoding 325 amino acids. The molecular weight was 35. 11 ku and the isoelectric point was 7. 59; there were 9 α helices,6 β folds,many randon coil and extension chain,containing one signal peptide and one transmembrane region,having a 2 amino acid conserved domains with typical beta-type carbonic anhydrase. Subcellular localization showed that the protein is most likely to appear in the chloroplast. The three-dimensional structure model of Fs CA1 was built by homologous modeling method,indicating that the homo-octamer of buckwheat CA and pea CA could match well,so it can be inferred that buckwheat CA is also homo-octamer. Real-time quantitative PCR was used to detect the expression of Fs CA1 in different organs of buckwheat.The results showed that Fs CA1 had the highest expression level in leaves,then in the stems,and the lowest in roots.
文摘Objective: to investigate the application effect of mental rehabilitation nursing in long-term hospitalization of elderly patients with chronic schizophrenia. Methods: a total of 62 long-term hospitalized elderly patients with chronic schizophrenia were divided into 31 cases in groups A and B by random number table method, and the study was carried out from January 2021 to December 2021. The study gave all patients routine nursing interventions, and group B added psychiatric rehabilitation nursing interventions and compared the effects of the interventions. Results: the IRROS and PSQI scale scores in group B were both lower than those in group A, the scores on SQLS and ADL scales were higher than those in group A, and the satisfaction of nursing care was also higher than that in group A, and the above comparisons were statistically significant (P〈0.05). Conclusion: the application of psychiatric rehabilitation nursing can significantly improve the rehabilitation effect of elderly patients with chronic schizophrenia, and actively improve the quality of sleep, as well as the ability to live in daily life and the quality of life.
文摘Grassroots power supply enterprises are at the forefront of providing customers with quality services, closely related to the interests of the people, and effectively do a good job of management within the scope of the team, so that the enterprise construction and team staff construction on the same line, conducive to the long-term development of the enterprise. The basic-level team is the basic part of power supply enterprise, is the foundation of the enterprise, thus promote team building mechanism can make the management more efficient, grass-roots team to be more perfect, to enriching the connotation of the team, strengthen the team construction, improve the core competitiveness of enterprises, the development of grassroots power supply enterprise has the vital role.
基金supported by the National Natural Science Foundation of China (61001116,61027003)the National Science and Technology Special Project (2009ZX03003-011)the International Scientific and Technological Cooperation Program (2010DFA11060,S2010GR0902)
文摘With rapid development of femtocell, dense deployment of femtocells in buildings will become an important study scenario. In this scenario, there exists severe inter-femtocell interference in the same building, which is revealed by our simulations in the 3-dimention （3D） scenario. If this type of interference is not well controlled, services to indoor users are bound to be deteriorated, especially for the femtocell edge users. Motivated by this problem, femto users＇ received interference model is constructed based on the scenario of 3D femtocell deployment. Then, a graph theory based in-building inter-femtocell coordination scheme is proposed, which includes three phases： establishment of femtocell interference graph, femtocell clustering and frequency resources allocation based on the proposed cluster influence circle. Finally, the simulation results confirm that the proposed scheme improves the femtocell average throughput by 22.4% and 26.2% in comparison with frequency universal reuse scheme and channel demodulation reliability of cell edge femto users. frequency hopping scheme respectively, and ensures the
