目的:探讨弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)患者的临床病理特征及其对预后评估作用。方法:选取本院2013年1月至2017年5月177例确诊为弥漫大B细胞淋巴瘤的初治患者,回顾性分析其临床病理特征及其对预后的影响。结...目的:探讨弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)患者的临床病理特征及其对预后评估作用。方法:选取本院2013年1月至2017年5月177例确诊为弥漫大B细胞淋巴瘤的初治患者,回顾性分析其临床病理特征及其对预后的影响。结果:单因素预后分析发现,分期Ⅲ-Ⅳ期(P=0.004,P=0.029)、ECOG评分2-4分(P=0.003,P=0.042)、结外受累> 1处(P=0.000,P=0.031)、异常升高的LDH值(P=0.000,P=0.046)为影响患者OS与PFS的危险因素;B症状(P=0.020)、异常升高的β2-MG值(P=0.020)仅影响患者OS。COX多因素分析显示,异常升高的β2-MG值(P=0.044)及分期(P=0.046)为影响患者OS的独立危险因素,分期(P=0.029)是影响患者PFS的独立因素。年龄调整的IPI(aaIPI)评分2-3分的患者与初治骨髓受累的患者OS(P=0.000,P=0.000)与PFS(P=0.013,P=0.000)更差。结论:异常升高的β2-MG值是影响弥漫大B细胞淋巴瘤患者OS的独立因素,分期是影响患者OS与PFS的独立因素。aaIPI评分2-3分患者与初治骨髓受累的患者OS与PFS更差。展开更多
In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechai...In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechain reaction(RQ-PCR),141 adult patients undergoing allo-HSCT between January 2008 and June 2010 were seriallymonitored by RQ-PCR for detecting CMV and guiding the preemptive therapy followed up to 180 days post-HSCT.Theresults showed that the incidence of CMV infection and CMV pneumonia was 81.5%and 2.9%respectively,whichmainly occurred within 2 months post-HSCT.Single-therapy with ganciclovir(GCV)for 63 patients or foscarnet 6patients was performed for preemptive therapy.The total efficacy was 87.8%,and the response patterns were different.CMV infection was more frequent in female patients(P=0.044),and those with aGVHD(P=0.043),using ATG orbasiliximab in conditioning regimens(P=0.049),as well as earlier in patients using ATG or basiliximab or those withaGVHD(P=0.007;P=0.000).The aGVHD,maximum load,positive times of CMV-DNA detection and therapyduration all correlated with the efficacy(P<0.05).It is concluded that the incidence of CMV infection is still high afterHSCT.Plasma RQ-PCR assay for CMV-DNA shows a strong correlation with the clinical outcome of CMV infection,which is useful and suitable for management of CMV infection in HSCT.展开更多
Objective: To investigate the effect of miR-197 regulation on the invasion and migration of uterine fibroid cells, and to analyze the relationship between miR-197 and the RON/PI3K signaling pathway. Methods: A total o...Objective: To investigate the effect of miR-197 regulation on the invasion and migration of uterine fibroid cells, and to analyze the relationship between miR-197 and the RON/PI3K signaling pathway. Methods: A total of 48 magnetic SD rats were selected, and were divided into four groups after adaptive feeding, with 12 rats in each group. Except for the blank group, the other three groups of rats were intramuscularly injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100 g·d)progesterone to construct a rat model of uterine fibroids. After successful modeling, the rats in the blank group and the model group were given 200 mg/(kg·d) normal saline by gavage, the rats in the up-regulation group were given 10 nmol/(L·d) pcDNA3.1-miR-197by gavage, and the rats in the down-regulation group were given 10 nmol/(L·d)miR-197-siRNA by gavage. After continuous treatment for 7 days, the expression levels of miR-197, the numbers of invaded and migrated uterine fibroids cells, and the expression levels of proteins related to the RON/PI3K signaling pathway in the four groups of rats were observed and compared. Results: There were statistically significant differences in the expression levels of miR-197 among the four groups(P<0.05), and the expression level of miR-197 in the up-regulation group was higher than that in the other three groups(P<0.05). There were statistically significant differences in the numbers of invaded and migrated cells among the four groups(P<0.05), the numbers of invaded and migrated cells in the up-regulation group were higher than those in the blank group and lower than those in the model group and the down-regulation group(P<0.05). There were statistically significant differences in the expression levels of RON and PI3K among the four groups(P<0.05), the expression level of RON in the up-regulation group was higher than that in the blank group and lower than that in the model group and the down-regulation group, and the expression level of PI3K was higher than that in the down-regulation group(P<0.05). Conclusion: Up-regulation of miR-197 can inhibit the invasion and migration of uterine fibroid cells in model rats, and the mechanism may be related to the RON/PI3K signaling pathway.展开更多
文摘目的:探讨弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)患者的临床病理特征及其对预后评估作用。方法:选取本院2013年1月至2017年5月177例确诊为弥漫大B细胞淋巴瘤的初治患者,回顾性分析其临床病理特征及其对预后的影响。结果:单因素预后分析发现,分期Ⅲ-Ⅳ期(P=0.004,P=0.029)、ECOG评分2-4分(P=0.003,P=0.042)、结外受累> 1处(P=0.000,P=0.031)、异常升高的LDH值(P=0.000,P=0.046)为影响患者OS与PFS的危险因素;B症状(P=0.020)、异常升高的β2-MG值(P=0.020)仅影响患者OS。COX多因素分析显示,异常升高的β2-MG值(P=0.044)及分期(P=0.046)为影响患者OS的独立危险因素,分期(P=0.029)是影响患者PFS的独立因素。年龄调整的IPI(aaIPI)评分2-3分的患者与初治骨髓受累的患者OS(P=0.000,P=0.000)与PFS(P=0.013,P=0.000)更差。结论:异常升高的β2-MG值是影响弥漫大B细胞淋巴瘤患者OS的独立因素,分期是影响患者OS与PFS的独立因素。aaIPI评分2-3分患者与初治骨髓受累的患者OS与PFS更差。
基金Key Program of Capital Development Foundation(编号2007-2040)
文摘In order to study the epidemiological characteristics of cytomegalovirus(CMV)infection in allogeneichematopoietic stem cell transplantation(allo-HSCT)recipients by means of plasma real time quantitative polymerasechain reaction(RQ-PCR),141 adult patients undergoing allo-HSCT between January 2008 and June 2010 were seriallymonitored by RQ-PCR for detecting CMV and guiding the preemptive therapy followed up to 180 days post-HSCT.Theresults showed that the incidence of CMV infection and CMV pneumonia was 81.5%and 2.9%respectively,whichmainly occurred within 2 months post-HSCT.Single-therapy with ganciclovir(GCV)for 63 patients or foscarnet 6patients was performed for preemptive therapy.The total efficacy was 87.8%,and the response patterns were different.CMV infection was more frequent in female patients(P=0.044),and those with aGVHD(P=0.043),using ATG orbasiliximab in conditioning regimens(P=0.049),as well as earlier in patients using ATG or basiliximab or those withaGVHD(P=0.007;P=0.000).The aGVHD,maximum load,positive times of CMV-DNA detection and therapyduration all correlated with the efficacy(P<0.05).It is concluded that the incidence of CMV infection is still high afterHSCT.Plasma RQ-PCR assay for CMV-DNA shows a strong correlation with the clinical outcome of CMV infection,which is useful and suitable for management of CMV infection in HSCT.
文摘Objective: To investigate the effect of miR-197 regulation on the invasion and migration of uterine fibroid cells, and to analyze the relationship between miR-197 and the RON/PI3K signaling pathway. Methods: A total of 48 magnetic SD rats were selected, and were divided into four groups after adaptive feeding, with 12 rats in each group. Except for the blank group, the other three groups of rats were intramuscularly injected with 0.05 mg/(100 g·d) estradiol benzoate and 0.5 mg/(100 g·d)progesterone to construct a rat model of uterine fibroids. After successful modeling, the rats in the blank group and the model group were given 200 mg/(kg·d) normal saline by gavage, the rats in the up-regulation group were given 10 nmol/(L·d) pcDNA3.1-miR-197by gavage, and the rats in the down-regulation group were given 10 nmol/(L·d)miR-197-siRNA by gavage. After continuous treatment for 7 days, the expression levels of miR-197, the numbers of invaded and migrated uterine fibroids cells, and the expression levels of proteins related to the RON/PI3K signaling pathway in the four groups of rats were observed and compared. Results: There were statistically significant differences in the expression levels of miR-197 among the four groups(P<0.05), and the expression level of miR-197 in the up-regulation group was higher than that in the other three groups(P<0.05). There were statistically significant differences in the numbers of invaded and migrated cells among the four groups(P<0.05), the numbers of invaded and migrated cells in the up-regulation group were higher than those in the blank group and lower than those in the model group and the down-regulation group(P<0.05). There were statistically significant differences in the expression levels of RON and PI3K among the four groups(P<0.05), the expression level of RON in the up-regulation group was higher than that in the blank group and lower than that in the model group and the down-regulation group, and the expression level of PI3K was higher than that in the down-regulation group(P<0.05). Conclusion: Up-regulation of miR-197 can inhibit the invasion and migration of uterine fibroid cells in model rats, and the mechanism may be related to the RON/PI3K signaling pathway.
