Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied.The experiments on long-term application of K fertilize...Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied.The experiments on long-term application of K fertilizer and wheat straw to soil in Hebei fluvo aquic soil and Shanxi brown soil in northern China were begun in 1992.The results showed that K fertilizer and straw could improve the yields of wheat and maize with the order of NPK+St〉NPK〉NP+St〉NP,and treatment of K fertilizer made a significant difference to NP,and the efficiency of K fertilizer in maize was higher than in wheat under rotation system of Hebei.In contrast with Shanxi,the wastage of soil potassium was a more serious issue in the rotation system in Hebei,only treatment of NPK+St showed a surplus of potassium and the others showed a wane.K fertilizer and straw could improve the content of water-soluble K,nonspecifically adsorbed K,non-exchangeable K,mineral K,and total K in contrast to NP;however,K fertilizer and straw reduce the proportion of mineral K and improve proportion of other forms of potassium in the two locating sites.Compared with the beginning of orientation,temporal variability character of soil K content and proportion showed a difference between the two soil types;furthermore,there was a decrease in the content of mineral K and total K simultaneously in the two locating sites.As a whole,the effect of K fertilizer applied to soil directly excelled to wheat straw to soil.Wheat straw to soil was an effective measure to complement potassium to increase crop yield and retard the decrease of soil K.展开更多
Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated th...Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.展开更多
Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities i...Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities in the greenhouses is relatively different from open fields in the same region. However, the responses of soil microbial biomass carbon （MBC） and nitrogen （MBN）, enzyme activities, microbial community composition, and yield to organic amendment are not well understood. Therefore, a 5-year greenhouse tomato （Solanum lycopersicum Mill.）-cucumber （Cucumis sativus L.） rotation experiment was conducted. The field experiment comprised 5 treatments： 4/4CN （CN, nitrogen in chemical fertilizer）, 3/4CN＋1/4MN （MN, nitrogen in pig manure）, 2/4CN＋2/4MN, 2/4CN＋1/4 MN＋1/4 SN （SN, nitrogen in corn straw） and 2/4CN＋2/4SN. The amounts of nitrogen （N）, phosphorus （P2O5）, and potassium （K2O） were equal in the five treatments. Starting with the fourth growing season, the optimal yield was obtained from soil treated with straw. MBC, MBN, phospholipid fatty acid （PLFA） profiles, and enzyme activities were significantly changed by 5 years of substitution with organic amendments. Redundancy analysis showed that MBC accounts for 89.5 and 52.3％ of the total enzyme activity and total community variability, respectively. The activities of phosphomonoesterase, N-acetyl-glucosaminidase, and urease, and the relative abundances of fungi, actinomycetes, and Gram-negative bacteria were significantly and positively related to vegetable yields. Considering the effects of organic amendments on soil microbial characteristics and vegetable yield, 2/4CN＋1/4MN＋1/4SN can improve soil quality and maintain sustainable high yield in greenhouse vegetable production.展开更多
Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrie...Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrients,and microbial characteristics within>2,0.25-2,0.053-0.25,and<0.053 mm aggregates,based on an eight-year field experiment in a greenhouse vegetable field in China.The field experiment includes four treatments:100%N fertilizer(CF),50%substitution of N frtilizer with manure(M),straw(S),and manure plus straw(MS).The amounts of nutrient(N,P20,and K20)input were equal in each treatment.Results showed higher values of mean weight diameter in organic amended soils(M,MS,and S,2.43-2.97)vs.CF-amended soils(1.99).Relative to CF treatment,organic amendments had positive effects on nutrient(i.e.,available N,P,and soil organic C(SOC))conditions,microbial(e.g,bacterial and fungal)growth,and EEAs in the>0.053 mm aggregates,but not in the<0.053 mm aggregates.The 0.25-0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity,while the<0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates,per SOC,available N,available P,and a series of enzyme activities.These results indicated that the 0.25-0.053 mm(<0.053 mm)aggregates provide suitable microhabitats for hydrolytic(oxidative)activity.Interestingly,we found that hydrolytic and oxidative activities were mainly impacted by fertilization(58.5%,P<0.01)and aggregate fractions(50.5%,P<0.01),respectively.The hydrolytic and oxidative activities were significantly(P<0.01)associated with nutrients(SOC and available N)and pH,electrical conductivity,respectively.Furthermore,SOC,available N,and available P closely(P<0.05)afected microbial communities within>0.25,0.25-0.053,and<0.053 mm aggregates,respectively.These findings provide several insights into microbial characteristics within aggregates under dfferent frilization modes in the greenhouse vegetable production system in China.展开更多
文摘Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied.The experiments on long-term application of K fertilizer and wheat straw to soil in Hebei fluvo aquic soil and Shanxi brown soil in northern China were begun in 1992.The results showed that K fertilizer and straw could improve the yields of wheat and maize with the order of NPK+St〉NPK〉NP+St〉NP,and treatment of K fertilizer made a significant difference to NP,and the efficiency of K fertilizer in maize was higher than in wheat under rotation system of Hebei.In contrast with Shanxi,the wastage of soil potassium was a more serious issue in the rotation system in Hebei,only treatment of NPK+St showed a surplus of potassium and the others showed a wane.K fertilizer and straw could improve the content of water-soluble K,nonspecifically adsorbed K,non-exchangeable K,mineral K,and total K in contrast to NP;however,K fertilizer and straw reduce the proportion of mineral K and improve proportion of other forms of potassium in the two locating sites.Compared with the beginning of orientation,temporal variability character of soil K content and proportion showed a difference between the two soil types;furthermore,there was a decrease in the content of mineral K and total K simultaneously in the two locating sites.As a whole,the effect of K fertilizer applied to soil directly excelled to wheat straw to soil.Wheat straw to soil was an effective measure to complement potassium to increase crop yield and retard the decrease of soil K.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-23-B04)the National Key Research and Development Program of China(2016YFD0201001)。
文摘Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.
基金supported by the National Key Research and Development Program of China (2016YFD0201001)the earmarked fund for China Agriculture Research System (CARS-23-B02)the Key Research and Development Program of Shandong Province,China (2017CXGC0206)
文摘Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities in the greenhouses is relatively different from open fields in the same region. However, the responses of soil microbial biomass carbon （MBC） and nitrogen （MBN）, enzyme activities, microbial community composition, and yield to organic amendment are not well understood. Therefore, a 5-year greenhouse tomato （Solanum lycopersicum Mill.）-cucumber （Cucumis sativus L.） rotation experiment was conducted. The field experiment comprised 5 treatments： 4/4CN （CN, nitrogen in chemical fertilizer）, 3/4CN＋1/4MN （MN, nitrogen in pig manure）, 2/4CN＋2/4MN, 2/4CN＋1/4 MN＋1/4 SN （SN, nitrogen in corn straw） and 2/4CN＋2/4SN. The amounts of nitrogen （N）, phosphorus （P2O5）, and potassium （K2O） were equal in the five treatments. Starting with the fourth growing season, the optimal yield was obtained from soil treated with straw. MBC, MBN, phospholipid fatty acid （PLFA） profiles, and enzyme activities were significantly changed by 5 years of substitution with organic amendments. Redundancy analysis showed that MBC accounts for 89.5 and 52.3％ of the total enzyme activity and total community variability, respectively. The activities of phosphomonoesterase, N-acetyl-glucosaminidase, and urease, and the relative abundances of fungi, actinomycetes, and Gram-negative bacteria were significantly and positively related to vegetable yields. Considering the effects of organic amendments on soil microbial characteristics and vegetable yield, 2/4CN＋1/4MN＋1/4SN can improve soil quality and maintain sustainable high yield in greenhouse vegetable production.
基金Fund for China Agriculture Research 3ystem(CAR3-23-B02)the National Key Research and Development Program of China(2016YFD0201001)the Key Research and Development Program of Shandong Province,China(2017CXGC0206).
文摘Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrients,and microbial characteristics within>2,0.25-2,0.053-0.25,and<0.053 mm aggregates,based on an eight-year field experiment in a greenhouse vegetable field in China.The field experiment includes four treatments:100%N fertilizer(CF),50%substitution of N frtilizer with manure(M),straw(S),and manure plus straw(MS).The amounts of nutrient(N,P20,and K20)input were equal in each treatment.Results showed higher values of mean weight diameter in organic amended soils(M,MS,and S,2.43-2.97)vs.CF-amended soils(1.99).Relative to CF treatment,organic amendments had positive effects on nutrient(i.e.,available N,P,and soil organic C(SOC))conditions,microbial(e.g,bacterial and fungal)growth,and EEAs in the>0.053 mm aggregates,but not in the<0.053 mm aggregates.The 0.25-0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity,while the<0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates,per SOC,available N,available P,and a series of enzyme activities.These results indicated that the 0.25-0.053 mm(<0.053 mm)aggregates provide suitable microhabitats for hydrolytic(oxidative)activity.Interestingly,we found that hydrolytic and oxidative activities were mainly impacted by fertilization(58.5%,P<0.01)and aggregate fractions(50.5%,P<0.01),respectively.The hydrolytic and oxidative activities were significantly(P<0.01)associated with nutrients(SOC and available N)and pH,electrical conductivity,respectively.Furthermore,SOC,available N,and available P closely(P<0.05)afected microbial communities within>0.25,0.25-0.053,and<0.053 mm aggregates,respectively.These findings provide several insights into microbial characteristics within aggregates under dfferent frilization modes in the greenhouse vegetable production system in China.
