Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α...Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis （TB） among patients with coal worker＇s pneumoconiosis （CWP）. Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism （PCR-RFLP）. Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher＇s exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls （2,2=5.44, P=-0.07）. But difference in frequency of the TNF-α-308 A allele was identified between them （2,2-5.14, P=0.02）. No significant difference in frequencies of the TNF-α-238 genotype and allele （P=0.23 and P=0.09, respectively） was found between cases and controls either, with combined （GG and AA） OR of 3.96 （95% confidence interval of 1.30-12.09） at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 （95% CI of 1.06-3.71） for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index （OR=4.92）, as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.展开更多
A wire-mesh catalyst coated by La0.8Sr0.2MnO3 was combined with a dielectric barrier discharge （DBD） reactor for toluene removal at atmospheric pressure. It was found that toluene removal efficiency and carbon dioxi...A wire-mesh catalyst coated by La0.8Sr0.2MnO3 was combined with a dielectric barrier discharge （DBD） reactor for toluene removal at atmospheric pressure. It was found that toluene removal efficiency and carbon dioxide selectivity were enhanced in the catalytic packed-bed reactor. In addition, ozone and nitrogen monoxide from the gas effluent byproducts decreased. This is the first time that ultrasound combined with plasma has been used for toluene removal. A synergistic effect on toluene removal was observed in the plasma-assisted ultrasound system. At the same time, the system increased toluene conversion and reduced ozone emission.展开更多
AIM: To explore the expression of BAG1 and tissue inhibitor of metalloproteinase 3 (TIMP3) in colon carcinoma and their correlation and clinicopathologic significance. METHODS: SABC immunohistochemistry was used to de...AIM: To explore the expression of BAG1 and tissue inhibitor of metalloproteinase 3 (TIMP3) in colon carcinoma and their correlation and clinicopathologic significance. METHODS: SABC immunohistochemistry was used to detect the expression of BAG1 and TIMP3 in 80 colon carcinoma tissues and 20 normal colonic mucosa. RESULTS: Positive rate of BAG1 in colon carcinoma tissue (80%) was notably higher compared to normal colonic mucosa (10%) (P < 0.05). However, no significant difference was observed in positive rate of TIMP3 in colon carcinoma tissue (43.75%) as compared with normal colonic mucosa (60%) (P > 0.05). Expression of BAG1 and TIMP3 was strongly associated with colon carcinoma differentiation, Duke's staging, lymph node metastasis and survival rate (P < 0.05), but not associated with gender and age. Moreover, BAG1 expression was not correlated with TIMP3. CONCLUSION: Our results suggest that over-expression of BAG1 or attenuated expression of TIMP3 may play an important role in genesis and development of colon carcinoma. The protein expression levels of BAG1 and TIMP3 are related to the malignant degree, infiltration and metastasis of colon carcinoma. BAG1 and TIMP3 might be new biological parameters in predicting invasion and metastasis of colon carcinoma.展开更多
BACKGROUND Diabetes is a clinically common chronic disease,and its incidence has been increasing in recent years.Diabetes is believed to accelerate the process of atherosclerosis in patients,and abnormal endothelial f...BACKGROUND Diabetes is a clinically common chronic disease,and its incidence has been increasing in recent years.Diabetes is believed to accelerate the process of atherosclerosis in patients,and abnormal endothelial function is an important factor leading to diabetic kidney damage.AIM To investigate the efficacy of ligliptin in the treatment of type 2 diabetes mellitus(T2DM)with early renal injury and its effect on serum endogenous hydrogen sulfide(H2S),endothelial cell particles,and endothelial function.METHODS From January 2018 to April 2019,110 patients with T2DM and early kidney injury treated at our hospital were divided into an observation group(receiving ligliptin treatment,n=54)and a control group(receiving gliquidone therapy,n=56).Blood glucose and renal function before and after treatment were compared between the two groups.RESULTS The differences in fasting blood glucose,2 h blood glucose,and glycated hemoglobin were not statistically significant between the two groups after treatment.The urinary albumin excretion rate after treatment in the ligliptin group was 70.32±11.21μg/min,which was significantly lower than that of the gliquidone group(P=0.000).Serum endogenous H2S and endothelial cell microparticles of the ligliptin treatment group were 40.04±8.82 mol/L and 133.40±34.39,respectively,which were significantly lower than those of the gliquidone treatment group(P=0.000 for both);endothelin-dependent diastolic function and nitric oxide after treatment in the ligliptin group were 7.98%±1.22%and 190.78±30.32 mol/L,significantly higher than those of the gliquidone treatment group(P=0.000 for both).CONCLUSION Ligliptin treatment of T2DM with early renal injury has the same glucoselowering effect as gliquidone treatment.Ligliptin treatment has a better effect and it can significantly improve the renal function and vascular endothelial function of patients,and reduce serum endogenous H2S and endothelial cell particle levels.展开更多
Objective： The aim of the study was to investigate the prospective study if treatment with arsenic trioxide （AS2O3） could enhance disease-free survival as adjuvant post-operative chemotherapy for gastric cancer pat...Objective： The aim of the study was to investigate the prospective study if treatment with arsenic trioxide （AS2O3） could enhance disease-free survival as adjuvant post-operative chemotherapy for gastric cancer patients and protect bone marrow from the negative effects of chemotherapy. Methods： 84 adults were randomized into two groups. Patients in treament group were treated with As2O3 and FOLFOX regimen, the other were administered with FOLFOX regimen only. Results： Four patients were withdrawn in treatment group after 3-4 cycles and the reasons were headache and fidgety （n = 2）, rhythmia （n = 1） and AST/ALT elevation （n = 1）, while 1 patient in control group after 4 cycles for neutropenia. In the treatment group, the median DFS was 28.34 months （95% CI, 25-33 months）. While in control group, the median DFS was 24.50 months （95% CI, 20-30 months）. This difference was not statistically significant （chi-square： 2.8885; P value： 0.0892）. Pa- tients in the same subgroup of node-positive was 29 in the treatment group and 32 in control group, respectively. The median DFS was 27.87 months （95% CI, 25-31 months） in the treatment group and 24.18 months （95% CI, 19-31 months） in the control group with promising statistical significance （HR 1.89; chi-square： 4.78; P value： 0.0287）. The most common grades 3-4 toxicity was leucopenia （n = 11） in control group and the difference was significant （chi-square： 3.9768, P value： 0.046） compared with that in treatment group （n = 4）. Conclusion： The combination of arsenic trioxide and FOLFOX regimen has a potential advantage of enhancing disease-free survival in patients with gastric cancer in nodal-positive status as post-operative chemotherapy, and protect bone marrow from the negative effects of chemotherapy.展开更多
Enhanced oil recovery(EOR)has been widely used to recover residual oil after the primary or secondary oil recovery processes.Compared to conventional methods,chemical EOR has demonstrated high oil recovery and low ope...Enhanced oil recovery(EOR)has been widely used to recover residual oil after the primary or secondary oil recovery processes.Compared to conventional methods,chemical EOR has demonstrated high oil recovery and low operational costs.Nanofluids have received extensive attention owing to their advantages of low cost,high oil recovery,and wide applicability.In recent years,nanofluids have been widely used in EOR processes.Moreover,several studies have focused on the role of nanofluids in the nanofluid EOR(N-EOR)process.However,the mechanisms related to N-EOR are unclear,and several of the mechanisms established are chaotic and contradictory.This review was conducted by considering heavy oil molecules/particle/surface micromechanics;nanofluidassisted EOR methods;multiscale,multiphase pore/core displacement experiments;and multiphase flow fluid-solid coupling simulations.Nanofluids can alter the wettability of minerals(particle/surface micromechanics),oil/water interfacial tension(heavy oil molecules/water micromechanics),and structural disjoining pressure(heavy oil molecules/particle/surface micromechanics).They can also cause viscosity reduction(micromechanics of heavy oil molecules).Nanofoam technology,nanoemulsion technology,and injected fluids were used during the EOR process.The mechanism of N-EOR is based on the nanoparticle adsorption effect.Nanoparticles can be adsorbed on mineral surfaces and alter the wettability of minerals from oil-wet to water-wet conditions.Nanoparticles can also be adsorbed on the oil/water surface,which alters the oil/water interfacial tension,resulting in the formation of emulsions.Asphaltenes are also adsorbed on the surface of nanoparticles,which reduces the asphaltene content in heavy oil,resulting in a decrease in the viscosity of oil,which helps in oil recovery.In previous studies,most researchers only focused on the results,and the nanoparticle adsorption properties have been ignored.This review presents the relationship between the adsorption properties of nanoparticles and the N-EOR mechanisms.The nanofluid behaviour during a multiphase core displacement process is also discussed,and the corresponding simulation is analysed.Finally,potential mechanisms and future directions of N-EOR are proposed.The findings of this study can further the understanding of N-EOR mechanisms from the perspective of heavy oil molecules/particle/surface micromechanics,as well as clarify the role of nanofluids in multiphase core displacement experiments and simulations.This review also presents limitations and bottlenecks,guiding researchers to develop methods to synthesise novel nanoparticles and conduct further research.展开更多
基金supported by grants from China National Programs for Science and Technology Development (Grant No. 2003BA712A11-24)Scientific Research Fund of North China Coal Medical College (Grant No. 2005-14)
文摘Objectives Tumor necrosis factor-α （TNF-α） may play an important role in host＇s immune response to mycobacterium tuberculosis （M. tuberculosis） infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis （TB） among patients with coal worker＇s pneumoconiosis （CWP）. Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism （PCR-RFLP）. Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher＇s exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls （2,2=5.44, P=-0.07）. But difference in frequency of the TNF-α-308 A allele was identified between them （2,2-5.14, P=0.02）. No significant difference in frequencies of the TNF-α-238 genotype and allele （P=0.23 and P=0.09, respectively） was found between cases and controls either, with combined （GG and AA） OR of 3.96 （95% confidence interval of 1.30-12.09） at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 （95% CI of 1.06-3.71） for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index （OR=4.92）, as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.
基金supported by the Program for Chang Jiang Scholars and Innovative Research Terms in Universities(No. IRT0936)the National Basic Research Program of China (No. 2009CB219905, 2009CB219907)
文摘A wire-mesh catalyst coated by La0.8Sr0.2MnO3 was combined with a dielectric barrier discharge （DBD） reactor for toluene removal at atmospheric pressure. It was found that toluene removal efficiency and carbon dioxide selectivity were enhanced in the catalytic packed-bed reactor. In addition, ozone and nitrogen monoxide from the gas effluent byproducts decreased. This is the first time that ultrasound combined with plasma has been used for toluene removal. A synergistic effect on toluene removal was observed in the plasma-assisted ultrasound system. At the same time, the system increased toluene conversion and reduced ozone emission.
文摘AIM: To explore the expression of BAG1 and tissue inhibitor of metalloproteinase 3 (TIMP3) in colon carcinoma and their correlation and clinicopathologic significance. METHODS: SABC immunohistochemistry was used to detect the expression of BAG1 and TIMP3 in 80 colon carcinoma tissues and 20 normal colonic mucosa. RESULTS: Positive rate of BAG1 in colon carcinoma tissue (80%) was notably higher compared to normal colonic mucosa (10%) (P < 0.05). However, no significant difference was observed in positive rate of TIMP3 in colon carcinoma tissue (43.75%) as compared with normal colonic mucosa (60%) (P > 0.05). Expression of BAG1 and TIMP3 was strongly associated with colon carcinoma differentiation, Duke's staging, lymph node metastasis and survival rate (P < 0.05), but not associated with gender and age. Moreover, BAG1 expression was not correlated with TIMP3. CONCLUSION: Our results suggest that over-expression of BAG1 or attenuated expression of TIMP3 may play an important role in genesis and development of colon carcinoma. The protein expression levels of BAG1 and TIMP3 are related to the malignant degree, infiltration and metastasis of colon carcinoma. BAG1 and TIMP3 might be new biological parameters in predicting invasion and metastasis of colon carcinoma.
文摘BACKGROUND Diabetes is a clinically common chronic disease,and its incidence has been increasing in recent years.Diabetes is believed to accelerate the process of atherosclerosis in patients,and abnormal endothelial function is an important factor leading to diabetic kidney damage.AIM To investigate the efficacy of ligliptin in the treatment of type 2 diabetes mellitus(T2DM)with early renal injury and its effect on serum endogenous hydrogen sulfide(H2S),endothelial cell particles,and endothelial function.METHODS From January 2018 to April 2019,110 patients with T2DM and early kidney injury treated at our hospital were divided into an observation group(receiving ligliptin treatment,n=54)and a control group(receiving gliquidone therapy,n=56).Blood glucose and renal function before and after treatment were compared between the two groups.RESULTS The differences in fasting blood glucose,2 h blood glucose,and glycated hemoglobin were not statistically significant between the two groups after treatment.The urinary albumin excretion rate after treatment in the ligliptin group was 70.32±11.21μg/min,which was significantly lower than that of the gliquidone group(P=0.000).Serum endogenous H2S and endothelial cell microparticles of the ligliptin treatment group were 40.04±8.82 mol/L and 133.40±34.39,respectively,which were significantly lower than those of the gliquidone treatment group(P=0.000 for both);endothelin-dependent diastolic function and nitric oxide after treatment in the ligliptin group were 7.98%±1.22%and 190.78±30.32 mol/L,significantly higher than those of the gliquidone treatment group(P=0.000 for both).CONCLUSION Ligliptin treatment of T2DM with early renal injury has the same glucoselowering effect as gliquidone treatment.Ligliptin treatment has a better effect and it can significantly improve the renal function and vascular endothelial function of patients,and reduce serum endogenous H2S and endothelial cell particle levels.
基金Supported by a grant from the Harbin Technologies R&D Program of China (No.2004AA9CS196-9)
文摘Objective： The aim of the study was to investigate the prospective study if treatment with arsenic trioxide （AS2O3） could enhance disease-free survival as adjuvant post-operative chemotherapy for gastric cancer patients and protect bone marrow from the negative effects of chemotherapy. Methods： 84 adults were randomized into two groups. Patients in treament group were treated with As2O3 and FOLFOX regimen, the other were administered with FOLFOX regimen only. Results： Four patients were withdrawn in treatment group after 3-4 cycles and the reasons were headache and fidgety （n = 2）, rhythmia （n = 1） and AST/ALT elevation （n = 1）, while 1 patient in control group after 4 cycles for neutropenia. In the treatment group, the median DFS was 28.34 months （95% CI, 25-33 months）. While in control group, the median DFS was 24.50 months （95% CI, 20-30 months）. This difference was not statistically significant （chi-square： 2.8885; P value： 0.0892）. Pa- tients in the same subgroup of node-positive was 29 in the treatment group and 32 in control group, respectively. The median DFS was 27.87 months （95% CI, 25-31 months） in the treatment group and 24.18 months （95% CI, 19-31 months） in the control group with promising statistical significance （HR 1.89; chi-square： 4.78; P value： 0.0287）. The most common grades 3-4 toxicity was leucopenia （n = 11） in control group and the difference was significant （chi-square： 3.9768, P value： 0.046） compared with that in treatment group （n = 4）. Conclusion： The combination of arsenic trioxide and FOLFOX regimen has a potential advantage of enhancing disease-free survival in patients with gastric cancer in nodal-positive status as post-operative chemotherapy, and protect bone marrow from the negative effects of chemotherapy.
基金funded by the 973 National Basic Research Program of China(Grant No.2015CB251403).
文摘Enhanced oil recovery(EOR)has been widely used to recover residual oil after the primary or secondary oil recovery processes.Compared to conventional methods,chemical EOR has demonstrated high oil recovery and low operational costs.Nanofluids have received extensive attention owing to their advantages of low cost,high oil recovery,and wide applicability.In recent years,nanofluids have been widely used in EOR processes.Moreover,several studies have focused on the role of nanofluids in the nanofluid EOR(N-EOR)process.However,the mechanisms related to N-EOR are unclear,and several of the mechanisms established are chaotic and contradictory.This review was conducted by considering heavy oil molecules/particle/surface micromechanics;nanofluidassisted EOR methods;multiscale,multiphase pore/core displacement experiments;and multiphase flow fluid-solid coupling simulations.Nanofluids can alter the wettability of minerals(particle/surface micromechanics),oil/water interfacial tension(heavy oil molecules/water micromechanics),and structural disjoining pressure(heavy oil molecules/particle/surface micromechanics).They can also cause viscosity reduction(micromechanics of heavy oil molecules).Nanofoam technology,nanoemulsion technology,and injected fluids were used during the EOR process.The mechanism of N-EOR is based on the nanoparticle adsorption effect.Nanoparticles can be adsorbed on mineral surfaces and alter the wettability of minerals from oil-wet to water-wet conditions.Nanoparticles can also be adsorbed on the oil/water surface,which alters the oil/water interfacial tension,resulting in the formation of emulsions.Asphaltenes are also adsorbed on the surface of nanoparticles,which reduces the asphaltene content in heavy oil,resulting in a decrease in the viscosity of oil,which helps in oil recovery.In previous studies,most researchers only focused on the results,and the nanoparticle adsorption properties have been ignored.This review presents the relationship between the adsorption properties of nanoparticles and the N-EOR mechanisms.The nanofluid behaviour during a multiphase core displacement process is also discussed,and the corresponding simulation is analysed.Finally,potential mechanisms and future directions of N-EOR are proposed.The findings of this study can further the understanding of N-EOR mechanisms from the perspective of heavy oil molecules/particle/surface micromechanics,as well as clarify the role of nanofluids in multiphase core displacement experiments and simulations.This review also presents limitations and bottlenecks,guiding researchers to develop methods to synthesise novel nanoparticles and conduct further research.
