Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms al...Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms along the entire coastline of Hainan Island have not been reported.This study examined the genetic diversity and population structure of the widely distributed oyster Saccostrea malabonensis around Hainan Island with analyzing mitochondrial COI gene sequences.The impacts of geographical,environmental and anthropogenic factors on genetic differentiation were also investigated.The results revealed a significant AT bias in the COI gene sequences,with transitions as the main mutation type.A total of 103 variable sites and 107 haplotypes were identified from480 COI sequences,with haplotype diversities from 0.067 to 0.782,and nucleotide diversities between 0.00011 and 0.00278.AMOVA analysis indicated that 86.65%of the variation occurred within one population while 13.35%among different populations.The average genetic distance across 16 populations was 0.00169,and the average genetic differentiation index was 0.13353.Distinct population patterns can be observed.The populations of Tonghai Village(THV)and Gangmen Mountain(GMM)in Lingshui showed similar genetic structures while those of Wanquan River Estuary(WQRE,Qionghai)and Wuzhizhou Island(WZZI,Sanya)displayed divergent evolutionary trends.Cluster analysis grouped the 480 individuals of S.malabonensis into six subpopulations.These findings are helpful for developing conservation strategies and genetic breeding programs,and are also helpful for understanding the evolutionary history of this oyster species in Hainan Island.展开更多
Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncodin...Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.展开更多
基金the Hainan Provincial Natural Science Foundation of China(No.325RC675)the Starting Research Fund from the Hainan University(No.KYQD(ZR)-21004)。
文摘Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms along the entire coastline of Hainan Island have not been reported.This study examined the genetic diversity and population structure of the widely distributed oyster Saccostrea malabonensis around Hainan Island with analyzing mitochondrial COI gene sequences.The impacts of geographical,environmental and anthropogenic factors on genetic differentiation were also investigated.The results revealed a significant AT bias in the COI gene sequences,with transitions as the main mutation type.A total of 103 variable sites and 107 haplotypes were identified from480 COI sequences,with haplotype diversities from 0.067 to 0.782,and nucleotide diversities between 0.00011 and 0.00278.AMOVA analysis indicated that 86.65%of the variation occurred within one population while 13.35%among different populations.The average genetic distance across 16 populations was 0.00169,and the average genetic differentiation index was 0.13353.Distinct population patterns can be observed.The populations of Tonghai Village(THV)and Gangmen Mountain(GMM)in Lingshui showed similar genetic structures while those of Wanquan River Estuary(WQRE,Qionghai)and Wuzhizhou Island(WZZI,Sanya)displayed divergent evolutionary trends.Cluster analysis grouped the 480 individuals of S.malabonensis into six subpopulations.These findings are helpful for developing conservation strategies and genetic breeding programs,and are also helpful for understanding the evolutionary history of this oyster species in Hainan Island.
基金supported by Jiangsu Commission of Health(M2020046).
文摘Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.
