Leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemos...Leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese superoxide dismutase expression in our tissue microarray was determined using immunohistochemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxorubicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 expression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expression produces a chemosensitivity in glioma.展开更多
Non-Hermitian systems have observed numerous novel phenomena and might lead to various applications.Unlike standard quantum physics,the conservation of energy guaranteed by the closed system is broken in the non-Hermi...Non-Hermitian systems have observed numerous novel phenomena and might lead to various applications.Unlike standard quantum physics,the conservation of energy guaranteed by the closed system is broken in the non-Hermitian system,and the energy can be exchanged between the system and the environment.Here we present a scheme for simulating the dissipative phase transition with an open quantum optical system.The competition between the coherent interaction and dissipation leads to the second-order phase transition.Furthermore,the quantum correlation in terms of squeezing is studied around the critical point.Our work may provide a new route to explore the non-Hermitian quantum physics with feasible techniques in experiments.展开更多
The dual-wavelength extreme ultraviolet camera(EUC)for the Queqiao-2 relay satellite of the Chang’E-7(CE-7)mission operates at 30.4 and 83.4 nm independently to simultaneously image the plasmasphere,magnetosheath,and...The dual-wavelength extreme ultraviolet camera(EUC)for the Queqiao-2 relay satellite of the Chang’E-7(CE-7)mission operates at 30.4 and 83.4 nm independently to simultaneously image the plasmasphere,magnetosheath,and ionospheric outflow from a lunar orbit.Each channel of the EUC is consisted of a concave multilayer mirror and a photoncounting imaging detector.This simple system achieves a large field of view(FOV),high spatial resolution,and optimized photon transmission efficiency to capture high-quality images of very weak extreme ultraviolet emissions in Earth space.Here we present the detailed design,tests,and calibrations of the EUC.Ground geometrical tests showed that the FOV was 20.2°for the 30.4 nm channel and 20.3°for the 83.4 nm channel,and the spatial resolution was 0.09°for both channels.Geometric distortion was corrected to be less than 1%.Pixels can be further binned on the ground to achieve higher sensitivity.Radiometric calibration results demonstrate that the sensitivity is 0.103 counts s^(-1)Rayleigh^(-1)pixel^(-1)at 30.4 nm channel and 0.061 counts s^(-1)Rayleigh^(-1)pixel^(-1)at 83.4 nm channel,with a calibration accuracy of~12%.The exposure can be flexibly set between 1–1500 s,with a longer exposure time achievable through time-delay integration during ground processing.The performance of the EUC fulfills the requirements of the scientific targets for the CE-7 mission and could significantly contribute to investigations of the solar wind-magnetosphere-ionosphere coupling system.展开更多
Harnessing the dynamics of complex quantum systems is an area of much interest and a quantum simulator has emerged as a promising platform to probe exotic topological phases.Since the flexibility offered by various co...Harnessing the dynamics of complex quantum systems is an area of much interest and a quantum simulator has emerged as a promising platform to probe exotic topological phases.Since the flexibility offered by various controllable quantum systems has helped gain insight into the quantum simulation of such complicated problems,an analog quantum simulator has recently shown its feasibility to tackle the problems of exploring topological phases.However,digital quantum simulation and the detection of topological phases still remain elusive.Here,we develop and experimentally realize the digital quantum simulation of topological phases with a solid-state quantum simulator at room temperature.Distinct from previous works dealing with static topological phases,the topological phases emulated here are Floquet topological phases.Furthermore,we also illustrate the procedure of digitally simulating a quantum quench and observing the nonequilibrium dynamics of Floquet topological phases.Using a quantum quench,the 0-andπ-energy topological invariants are unambiguously detected through measuring time-averaged spin polarizations.We believe our experiment opens up a new avenue to digitally simulate and detect Floquet topological phases with fast-developed programmable quantum simulators.展开更多
Spin-squeezed state is a many-body entangled state of great interest for precision measurements.Although the absolute sensitivity at the standard quantum limit is better for a larger atom number,the greater dominance ...Spin-squeezed state is a many-body entangled state of great interest for precision measurements.Although the absolute sensitivity at the standard quantum limit is better for a larger atom number,the greater dominance of classical noises over atom projection noise makes it harder to achieve spin squeezing.Here,we show both theoretically and experimentally that adiabatic pulse control of the pump field in state preparation is indispensable to sufficient noise suppression,which is the prerequisite for spin squeezing.This technique is generally applicable to spin-squeezing experiments involving a large ensemble and is thus of significance for quantum metrolog yapplications.展开更多
Erythropoietin （EPO） genomic gene was cloned and its expression vector pOP13/EPO was constructed. CHOK12 cell was transfected by this vector using lipofectin method. A stable expression cell strain C10 cell with the...Erythropoietin （EPO） genomic gene was cloned and its expression vector pOP13/EPO was constructed. CHOK12 cell was transfected by this vector using lipofectin method. A stable expression cell strain C10 cell with the EPO production at 160IU/d in 10\+6 cells were obtained at 400 μg/mL G418. Based on the C10 cell, another vector pHY/dhfr （dihydrofolate reductase） that carries a dhfr gene and a selecting marker of hygromycin B resistant gene was transferred to this cell. Several cell clones were obtained at 200 μg/mL hygromycin B. These cell clones that can express both EPO gene and exogenous dhfr gene were selected under the progressively increased concentration to 1 μmol methotrexate（MTX）. Some high EPO expression cell clones were obtained, the highest expression was 2 400 IU/d in 10\+6 cells, 15 times higher than that without MTX pressure. Then, a method of EPO high expression by using undhfr negative cell was primarily established. EPO bioactivity was found by using TF1 cell.展开更多
Normal-mode splitting is a hallmark of strong coupling between two coupled harmonic oscillators.Here,we report the realization of strong coupling in the optically-levitated nanoparticle system via feedback.A silica na...Normal-mode splitting is a hallmark of strong coupling between two coupled harmonic oscillators.Here,we report the realization of strong coupling in the optically-levitated nanoparticle system via feedback.A silica nanoparticle is trapped by a tightly focused laser travelling in free space,which is regarded as a harmonic oscillators.An external electric oscillator is then phase-locked to the nanoparticle’s motion as another harmonic oscillator,which is modulated on the trapping laser to feedback and interact with the nanoparticle.Therefore,a highly manipulatable coupled-harmonic oscillator system is built in our platform and the normal-mode splitting is realized with strong coupling in both Hermitian and non-Hermitian cases.Moreover,since the coupling between the two harmonic oscillators induced by the feedback is flexibly manipulated,the normal-mode splitting following the cooling or heating effect is simultaneously observed.This method could be useful for further studying quantum mechanical Hamiltonian and non-Hermitian phenomena of an optically-levitated nanoparticle.展开更多
基金the National Natural Science Foundation of China, No. 30973072Independent Research Project of Wuhan University for Graduate Students, No. 201130202020001Fundamental Research Funds for the Central Universities
文摘Leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） is an anti-oncogene. LRIG1 is correlated with Bcl-2 in ependymomas. Decreased Bcl-2 and manganese superoxide dismutase expression can improve the chemosensitivity of glioma. In the present study, a tissue microarray of human brain astrocytomas was constructed. To investigate the relationship of LRIG1 with Bcl-2 and manganese superoxide dismutase, LRIG1, Bcl-2 and manganese superoxide dismutase expression in our tissue microarray was determined using immunohistochemistry. In addition, we constructed the LRIG1-U251 cell line, and its responses to doxorubicin and temozolomide were detected using the MTT assay. Results showed that LRIG1 expression was significantly negatively correlated with Bcl-2 and manganese superoxide dismutase expression in glioma. Also, proliferation of LRIG1-U251 cells exposed to doxorubicin or temozolomide was significantly inhibited, i.e. in the LRIG1-U251 cell line, the chemosensitivity to doxorubicin and temozolomide was increased. This indicates that increased LRIG1 expression produces a chemosensitivity in glioma.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 61925503, 11874038, and 11654002)the Key Project of the National Key R&D Program of China (Grant Nos. 2016YFA0301402 and 2020YFA0309400)+2 种基金the Program for the Innovative Talents of Higher Education Institutions of Shanxithe Program for Sanjin Scholars of Shanxi Provincethe Fund for Shanxi “1331 Project” Key Subjects Construction
文摘Non-Hermitian systems have observed numerous novel phenomena and might lead to various applications.Unlike standard quantum physics,the conservation of energy guaranteed by the closed system is broken in the non-Hermitian system,and the energy can be exchanged between the system and the environment.Here we present a scheme for simulating the dissipative phase transition with an open quantum optical system.The competition between the coherent interaction and dissipation leads to the second-order phase transition.Furthermore,the quantum correlation in terms of squeezing is studied around the critical point.Our work may provide a new route to explore the non-Hermitian quantum physics with feasible techniques in experiments.
基金supported by the Chinese Lunar Exploration Projectsupported by the National Natural Science Foundation of China(Grant Nos.42222408&42441809)+1 种基金the National Key Research and Development Program of China(Grant No.2021YFA0718600)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.Y2021027)。
文摘The dual-wavelength extreme ultraviolet camera(EUC)for the Queqiao-2 relay satellite of the Chang’E-7(CE-7)mission operates at 30.4 and 83.4 nm independently to simultaneously image the plasmasphere,magnetosheath,and ionospheric outflow from a lunar orbit.Each channel of the EUC is consisted of a concave multilayer mirror and a photoncounting imaging detector.This simple system achieves a large field of view(FOV),high spatial resolution,and optimized photon transmission efficiency to capture high-quality images of very weak extreme ultraviolet emissions in Earth space.Here we present the detailed design,tests,and calibrations of the EUC.Ground geometrical tests showed that the FOV was 20.2°for the 30.4 nm channel and 20.3°for the 83.4 nm channel,and the spatial resolution was 0.09°for both channels.Geometric distortion was corrected to be less than 1%.Pixels can be further binned on the ground to achieve higher sensitivity.Radiometric calibration results demonstrate that the sensitivity is 0.103 counts s^(-1)Rayleigh^(-1)pixel^(-1)at 30.4 nm channel and 0.061 counts s^(-1)Rayleigh^(-1)pixel^(-1)at 83.4 nm channel,with a calibration accuracy of~12%.The exposure can be flexibly set between 1–1500 s,with a longer exposure time achievable through time-delay integration during ground processing.The performance of the EUC fulfills the requirements of the scientific targets for the CE-7 mission and could significantly contribute to investigations of the solar wind-magnetosphere-ionosphere coupling system.
基金National Key Research and Development Program of China(2020YFA0309400,2017YFA0304203,2018YFA0306600,2018YFF01012500)Newton Fund(NF170876)+4 种基金Fund for Shanxi 1331 Project Key Subjects Construction and 111 Project(D18001)PCSIRT(IRT-17R70)National Natural Science Foundation of China(11604069,11904070,12074234)Fundamental Research Funds for the Central Universities(PA2019GDQT0023)Program of State Key Laboratory of Quantum Optics and Quantum Optics Devices(KF201802)。
文摘Harnessing the dynamics of complex quantum systems is an area of much interest and a quantum simulator has emerged as a promising platform to probe exotic topological phases.Since the flexibility offered by various controllable quantum systems has helped gain insight into the quantum simulation of such complicated problems,an analog quantum simulator has recently shown its feasibility to tackle the problems of exploring topological phases.However,digital quantum simulation and the detection of topological phases still remain elusive.Here,we develop and experimentally realize the digital quantum simulation of topological phases with a solid-state quantum simulator at room temperature.Distinct from previous works dealing with static topological phases,the topological phases emulated here are Floquet topological phases.Furthermore,we also illustrate the procedure of digitally simulating a quantum quench and observing the nonequilibrium dynamics of Floquet topological phases.Using a quantum quench,the 0-andπ-energy topological invariants are unambiguously detected through measuring time-averaged spin polarizations.We believe our experiment opens up a new avenue to digitally simulate and detect Floquet topological phases with fast-developed programmable quantum simulators.
基金National Key Research and Development Program of China(2017YFA0304204,2016YFA0302000)National Natural Science Foundation of China(12027806,91636107)+2 种基金Royal Society Newton International Fellowship(NF170876)PCSIRT(IRT_17R70)1331KSC。
文摘Spin-squeezed state is a many-body entangled state of great interest for precision measurements.Although the absolute sensitivity at the standard quantum limit is better for a larger atom number,the greater dominance of classical noises over atom projection noise makes it harder to achieve spin squeezing.Here,we show both theoretically and experimentally that adiabatic pulse control of the pump field in state preparation is indispensable to sufficient noise suppression,which is the prerequisite for spin squeezing.This technique is generally applicable to spin-squeezing experiments involving a large ensemble and is thus of significance for quantum metrolog yapplications.
文摘Erythropoietin （EPO） genomic gene was cloned and its expression vector pOP13/EPO was constructed. CHOK12 cell was transfected by this vector using lipofectin method. A stable expression cell strain C10 cell with the EPO production at 160IU/d in 10\+6 cells were obtained at 400 μg/mL G418. Based on the C10 cell, another vector pHY/dhfr （dihydrofolate reductase） that carries a dhfr gene and a selecting marker of hygromycin B resistant gene was transferred to this cell. Several cell clones were obtained at 200 μg/mL hygromycin B. These cell clones that can express both EPO gene and exogenous dhfr gene were selected under the progressively increased concentration to 1 μmol methotrexate（MTX）. Some high EPO expression cell clones were obtained, the highest expression was 2 400 IU/d in 10\+6 cells, 15 times higher than that without MTX pressure. Then, a method of EPO high expression by using undhfr negative cell was primarily established. EPO bioactivity was found by using TF1 cell.
基金supported by National Natural Science Foundation of China(Grant No.11234008,11361161002,61975101,61571276,11654002).
文摘Normal-mode splitting is a hallmark of strong coupling between two coupled harmonic oscillators.Here,we report the realization of strong coupling in the optically-levitated nanoparticle system via feedback.A silica nanoparticle is trapped by a tightly focused laser travelling in free space,which is regarded as a harmonic oscillators.An external electric oscillator is then phase-locked to the nanoparticle’s motion as another harmonic oscillator,which is modulated on the trapping laser to feedback and interact with the nanoparticle.Therefore,a highly manipulatable coupled-harmonic oscillator system is built in our platform and the normal-mode splitting is realized with strong coupling in both Hermitian and non-Hermitian cases.Moreover,since the coupling between the two harmonic oscillators induced by the feedback is flexibly manipulated,the normal-mode splitting following the cooling or heating effect is simultaneously observed.This method could be useful for further studying quantum mechanical Hamiltonian and non-Hermitian phenomena of an optically-levitated nanoparticle.
