Domain of unknown function 579(DUF579)家族广泛存在于真核生物中,该家族在次生细胞壁发育和木聚糖生物合成中发挥着重要的作用。目前,还没有研究系统报道过甘蓝型油菜BnaDUF579基因家族。本研究通过生物信息学方法对甘蓝型油菜BnaDUF...Domain of unknown function 579(DUF579)家族广泛存在于真核生物中,该家族在次生细胞壁发育和木聚糖生物合成中发挥着重要的作用。目前,还没有研究系统报道过甘蓝型油菜BnaDUF579基因家族。本研究通过生物信息学方法对甘蓝型油菜BnaDUF579基因家族进行进化树构建、基因结构和保守基序分析、染色体分布和共线性分析、组织表达分析、启动子顺式作用元件分析。结果表明,甘蓝型油菜BnaDUF579家族成员有31个,其中的24个成员都只有1个外显子、没有内含子。这些基因分为4个分支,包括Group1、Group2、Group3和Group4。同一分支内的成员具有相似的基序组成,但不同分支之间的成员在基序组成上存在明显差异。该家族与白菜亲缘关系较远、与甘蓝亲缘关系更近。BnaDUF579基因家族主要在油菜茎、根、角果和种子中表达,其启动子顺式作用元件涉及激素反应、对非生物胁迫的反应、组织发育、光响应等生理过程。以上研究结果丰富了对甘蓝型油菜BnaDUF579基因的认识,为油菜BnaDUF579基因的功能研究奠定了基础。展开更多
A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,...A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.展开更多
文摘Domain of unknown function 579(DUF579)家族广泛存在于真核生物中,该家族在次生细胞壁发育和木聚糖生物合成中发挥着重要的作用。目前,还没有研究系统报道过甘蓝型油菜BnaDUF579基因家族。本研究通过生物信息学方法对甘蓝型油菜BnaDUF579基因家族进行进化树构建、基因结构和保守基序分析、染色体分布和共线性分析、组织表达分析、启动子顺式作用元件分析。结果表明,甘蓝型油菜BnaDUF579家族成员有31个,其中的24个成员都只有1个外显子、没有内含子。这些基因分为4个分支,包括Group1、Group2、Group3和Group4。同一分支内的成员具有相似的基序组成,但不同分支之间的成员在基序组成上存在明显差异。该家族与白菜亲缘关系较远、与甘蓝亲缘关系更近。BnaDUF579基因家族主要在油菜茎、根、角果和种子中表达,其启动子顺式作用元件涉及激素反应、对非生物胁迫的反应、组织发育、光响应等生理过程。以上研究结果丰富了对甘蓝型油菜BnaDUF579基因的认识,为油菜BnaDUF579基因的功能研究奠定了基础。
基金Supported by the National Natural Science Foundation of China(No.30725045)the Special Program for New Drug Innovation of the Ministry of Science and Technology,China(Nos.2009ZX09311-001 and 2008ZX09101-Z-029)+2 种基金China Postdoctoral Science Foundation(No.20080441304)Shanghai Leading Academic Discipline Project,China(No.B906)the Scientific Foundation of Shanghai City,China(Nos.07DZ19728,09DZ1975700 and 09DZ1971500)
文摘A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.
