传统的开关器件脉频调制(Pulse Frequency Modulation,PFM)控制方法,存在开关频率高、频率不固定且稳定性能不好的缺点,使得它在APF的应用中增加了滤波器的设计难度。为此提出了以最优占空比为原则的改进脉频调制方法。该方法通过最小...传统的开关器件脉频调制(Pulse Frequency Modulation,PFM)控制方法,存在开关频率高、频率不固定且稳定性能不好的缺点,使得它在APF的应用中增加了滤波器的设计难度。为此提出了以最优占空比为原则的改进脉频调制方法。该方法通过最小二乘法获得最优占空比,并依此合理选择控制参数,且在控制器中,引入电网及补偿电压的实时特性,参与开关器件的导通时间、关断时间及滞环环宽的控制,从而实现定频、降损、提高控制精度及动态性能。通过对PFM的传统与改进控制策略的仿真与实验,验证了所提方法的有效性,且该方法易于控制电路的设计。展开更多
An oligopeptide permease A(OppA)was purified from the extracellular product of Vibrio harveyi SF-1.The molecular weight of the purified protein was estimated to be 58 kDa on SDS-PAGE.The purified protein showed phosph...An oligopeptide permease A(OppA)was purified from the extracellular product of Vibrio harveyi SF-1.The molecular weight of the purified protein was estimated to be 58 kDa on SDS-PAGE.The purified protein showed phospholipase C activity at the optimal values of temperature 50℃ and pH 8.0.The enzymatic activity decreased when the temperature increased to 40℃.The N-terminal sequence of the purified protein was determined as ADVPAGTKLA,which is similar to that of OppA.The OppA pre-cursor gene was cloned from the genome of V.harveyi SF-1.The gene consisted of 1665 base pairs and encoded a 554 amino acid polypeptide,which showed a high similarity to those of the OppAs of V.harveyi and other Vibrio species.The gene was subcloned into pET-28a(+)and expressed in Escherichia coli.The expressed recombinant protein was purified by Ni-NTA metal affinity chro-matography.The expressed recombinant protein showed a 58 kDa band on SDS-PAGE and exhibited phospholipase C activity with the optima of temperature 50℃ and pH 8.0.The purified protein was toxic to the flounder gill cells.An OppA mutant of V.harveyi SF-1 was constructed by homologous recombination.The mutant strain was less virulent when it was intraperitoneally inoculated to zebra fish,with the LD50 of 5.46×105 CFU fish-1,compared to 3.11×104 CFU fish-1 of the wild-type strain,which indicated that the OppA might play an important role in the pathogenicity of V.harveyi.展开更多
文摘传统的开关器件脉频调制(Pulse Frequency Modulation,PFM)控制方法,存在开关频率高、频率不固定且稳定性能不好的缺点,使得它在APF的应用中增加了滤波器的设计难度。为此提出了以最优占空比为原则的改进脉频调制方法。该方法通过最小二乘法获得最优占空比,并依此合理选择控制参数,且在控制器中,引入电网及补偿电压的实时特性,参与开关器件的导通时间、关断时间及滞环环宽的控制,从而实现定频、降损、提高控制精度及动态性能。通过对PFM的传统与改进控制策略的仿真与实验,验证了所提方法的有效性,且该方法易于控制电路的设计。
基金supported by grants of the National High-Tech R and D Program (2007AA09Z416)the Natural Science Foundation of China (30972275)
文摘An oligopeptide permease A(OppA)was purified from the extracellular product of Vibrio harveyi SF-1.The molecular weight of the purified protein was estimated to be 58 kDa on SDS-PAGE.The purified protein showed phospholipase C activity at the optimal values of temperature 50℃ and pH 8.0.The enzymatic activity decreased when the temperature increased to 40℃.The N-terminal sequence of the purified protein was determined as ADVPAGTKLA,which is similar to that of OppA.The OppA pre-cursor gene was cloned from the genome of V.harveyi SF-1.The gene consisted of 1665 base pairs and encoded a 554 amino acid polypeptide,which showed a high similarity to those of the OppAs of V.harveyi and other Vibrio species.The gene was subcloned into pET-28a(+)and expressed in Escherichia coli.The expressed recombinant protein was purified by Ni-NTA metal affinity chro-matography.The expressed recombinant protein showed a 58 kDa band on SDS-PAGE and exhibited phospholipase C activity with the optima of temperature 50℃ and pH 8.0.The purified protein was toxic to the flounder gill cells.An OppA mutant of V.harveyi SF-1 was constructed by homologous recombination.The mutant strain was less virulent when it was intraperitoneally inoculated to zebra fish,with the LD50 of 5.46×105 CFU fish-1,compared to 3.11×104 CFU fish-1 of the wild-type strain,which indicated that the OppA might play an important role in the pathogenicity of V.harveyi.
