An increasing incidence and distribution of Verticillium wilt has occurred in the last few years in newly established olive orchards in Nineveh province,northern Iraq.This spread of the disease may result from use of ...An increasing incidence and distribution of Verticillium wilt has occurred in the last few years in newly established olive orchards in Nineveh province,northern Iraq.This spread of the disease may result from use of Verticillium dahliae infected planting material.In this work genetic variation among the isolates of F.dahliae from Iraq and Jordan were analyzed by using Random Amplified Polymorphic DNA(RAPD)marker techniques for the first time in Iraq.The results of using 85 primers showed that 17 primers(Z1,Z19,T13,R10,RI5,RI6,F5,F6,FI0,AI5,AI9,B12,M1,M2,M3,M4 and M5)showed significant results with 30 isolates of I,:dahliae using RAPD and the best results were found with the primers M1,M2,M3,M4 and M5 which exhibited a clear difference between isolates.The genetic similarity between isolates ranged from 5%in the two isolates Alshallalat/2 and Tomato/Alkerak to 88%for Bashaika 1 and 2,while the average genetic variation between all isolates reached 58%.展开更多
文摘An increasing incidence and distribution of Verticillium wilt has occurred in the last few years in newly established olive orchards in Nineveh province,northern Iraq.This spread of the disease may result from use of Verticillium dahliae infected planting material.In this work genetic variation among the isolates of F.dahliae from Iraq and Jordan were analyzed by using Random Amplified Polymorphic DNA(RAPD)marker techniques for the first time in Iraq.The results of using 85 primers showed that 17 primers(Z1,Z19,T13,R10,RI5,RI6,F5,F6,FI0,AI5,AI9,B12,M1,M2,M3,M4 and M5)showed significant results with 30 isolates of I,:dahliae using RAPD and the best results were found with the primers M1,M2,M3,M4 and M5 which exhibited a clear difference between isolates.The genetic similarity between isolates ranged from 5%in the two isolates Alshallalat/2 and Tomato/Alkerak to 88%for Bashaika 1 and 2,while the average genetic variation between all isolates reached 58%.
