Methionine restriction(MR)is an effective dietary strategy to regulate energy metabolism and alleviate oxidative stress and inflammation in the body,especially in the middle-aged and elderly population.However,the hig...Methionine restriction(MR)is an effective dietary strategy to regulate energy metabolism and alleviate oxidative stress and inflammation in the body,especially in the middle-aged and elderly population.However,the high methionine content of meat products makes this dietary strategy impossible to combine with protein supplementation and MR.Highland barley(HB),a low-methionine cereal,not only provides the body with protein but also has improved glucose metabolism and antioxidant and anti-inflammatory properties.Therefore,this study evaluated the feasibility of HB as a source of methionine-restricted dietary protein and the potential mechanisms.Middle-aged C57BL/6J mice were fed a control diet(CON),a high-fat diet(HFD),a whole-grain HB high-fat diet(HBHF),or a HBHF+methionine diet(HBHFmet)for 25 weeks.The results showed that the HBHF could keep the body weight,fasting glucose,insulin,homeostasis model assessment of insulin resistance(HOMA-IR),blood lipids,inflammation,and oxidative stress of HFD mice at normal levels.Compared with the HFD groups,HBHF inhibited pancreatic cell apoptosis and improved insulin secretion while improving hepatic and skeletal muscle glucose metabolism.However,these efficacies were attenuated in HBHFmet group mice.These findings suggest that HBHF has an MR strategy.展开更多
Non-alcoholic fatty liver disease(NAFLD)is a leading cause of chronic liver disease,closely associated with dyslipidemia,obesity,and diabetes.The gut-liver axis(GLA)plays a pivotal role in the pathogenesis of NAFLD,wi...Non-alcoholic fatty liver disease(NAFLD)is a leading cause of chronic liver disease,closely associated with dyslipidemia,obesity,and diabetes.The gut-liver axis(GLA)plays a pivotal role in the pathogenesis of NAFLD,with dietary factors acting as key regulators that shape gut microbiota and mediate GLA,consequently impacting NAFLD and host health.While reviews on specific dietary patterns or components and their relationship with NAFLD have been published in the past five years,a comprehensive summary of these dietary factors and their effects on NAFLD via the GLA mechanism is lacking.This review aims to provide a comprehensive overview of this mechanism by analyzing GLA-dependent metabolites,including bile acids,short-chain fatty acids,choline,trimethylamine-N-oxide,endotoxin,ammonia,and amino acids.Additionally,the review summarizes the various dietary factors that influence NAFLD through the GLA,encompassing dietary patterns such as western-style diet,Mediterranean diet,methionine-restricted diet,calorie-restricted diet,and protein-restricted diet,as well as oral microecological agents such as probiotics,prebiotics,synbiotics,and postbiotics.The majority of these dietary interventions,except for the western-style diet and long-term protein-restricted diet,demonstrate positive effects on improving NAFLD.Furthermore,synbiotics and postbiotics have emerged as promising agents for ameliorating NAFLD-associated liver inflammation and lipid accumulation.In conclusion,this review provides a detailed understanding of the GLA mechanism and presents multiple possibilities for the nutritional treatment of NAFLD.展开更多
A methionine-restricted diet(MRD)has been shown to reduce body fat deposition and improve obesityassociated metabolic disorders,with emerging evidence implicating the gut microbiota in mediating these effects.This stu...A methionine-restricted diet(MRD)has been shown to reduce body fat deposition and improve obesityassociated metabolic disorders,with emerging evidence implicating the gut microbiota in mediating these effects.This study investigated the effects and underlying mechanisms of fecal microbiota transplantation(FMT)from MRD mouse donors on body fat deposition in obese mice.Five-week-old male C57BL/6J mice were continuously fed a high-fat diet(HFD,0.86%methionine+24%fat)to induce obesity,and then randomly divided into three groups.These groups received FMT from 23-week-old male C57BL/6J mice donors continuously fed with normal diet(0.86%methionine+4.2%fat),HFD,or MRD(0.17%methionine+24%fat)for eight weeks,respectively.Our results indicate that FMT from MRD mouse donors reduced body weight,plasma lipid levels,and percentage fat mass.Moreover,FMT from MRD mouse donors elevated the abundance of short-chain fatty acid(SCFA)-producing bacteria while decreasing pro-inflammatory bacteria levels in cecal contents.In addition,systemic metabolic processes were altered,featuring heightened SCFA production and enhanced energy metabolism.FMT from MRD mouse donors further facilitated fat catabolism,inhibited fat anabolism,and decreased lipid levels and lipid accumulation in liver.Meanwhile,FMT from MRD mouse donors also promoted fat browning,decreased adipocyte volume,and reduced fat deposition in adipose tissues.These findings underscore the potential of FMT from MRD mouse donors to improve the gut microbiota and reduce body fat deposition in obese mice.Importantly,our results elucidate that MRD intervention in obesity is,in part,mediated by regulating intestinal microbial composition.展开更多
基金supported by the 12th Five-Year Plan for Science and Technology Development of China(2012BAD33B05).
文摘Methionine restriction(MR)is an effective dietary strategy to regulate energy metabolism and alleviate oxidative stress and inflammation in the body,especially in the middle-aged and elderly population.However,the high methionine content of meat products makes this dietary strategy impossible to combine with protein supplementation and MR.Highland barley(HB),a low-methionine cereal,not only provides the body with protein but also has improved glucose metabolism and antioxidant and anti-inflammatory properties.Therefore,this study evaluated the feasibility of HB as a source of methionine-restricted dietary protein and the potential mechanisms.Middle-aged C57BL/6J mice were fed a control diet(CON),a high-fat diet(HFD),a whole-grain HB high-fat diet(HBHF),or a HBHF+methionine diet(HBHFmet)for 25 weeks.The results showed that the HBHF could keep the body weight,fasting glucose,insulin,homeostasis model assessment of insulin resistance(HOMA-IR),blood lipids,inflammation,and oxidative stress of HFD mice at normal levels.Compared with the HFD groups,HBHF inhibited pancreatic cell apoptosis and improved insulin secretion while improving hepatic and skeletal muscle glucose metabolism.However,these efficacies were attenuated in HBHFmet group mice.These findings suggest that HBHF has an MR strategy.
基金supported by National Natural Science Foundation of China(No.82103836)the Natural Science Foundation of Zhejiang Province(No.LQ22H260002)+1 种基金the Introduction of Talents Research Project of Ningbo University(No.ZX2022000093)Open Project Program of State Key Laboratory of Food Science and Resources,Jiangnan University,China(No.SKLF-KF-202111).
文摘Non-alcoholic fatty liver disease(NAFLD)is a leading cause of chronic liver disease,closely associated with dyslipidemia,obesity,and diabetes.The gut-liver axis(GLA)plays a pivotal role in the pathogenesis of NAFLD,with dietary factors acting as key regulators that shape gut microbiota and mediate GLA,consequently impacting NAFLD and host health.While reviews on specific dietary patterns or components and their relationship with NAFLD have been published in the past five years,a comprehensive summary of these dietary factors and their effects on NAFLD via the GLA mechanism is lacking.This review aims to provide a comprehensive overview of this mechanism by analyzing GLA-dependent metabolites,including bile acids,short-chain fatty acids,choline,trimethylamine-N-oxide,endotoxin,ammonia,and amino acids.Additionally,the review summarizes the various dietary factors that influence NAFLD through the GLA,encompassing dietary patterns such as western-style diet,Mediterranean diet,methionine-restricted diet,calorie-restricted diet,and protein-restricted diet,as well as oral microecological agents such as probiotics,prebiotics,synbiotics,and postbiotics.The majority of these dietary interventions,except for the western-style diet and long-term protein-restricted diet,demonstrate positive effects on improving NAFLD.Furthermore,synbiotics and postbiotics have emerged as promising agents for ameliorating NAFLD-associated liver inflammation and lipid accumulation.In conclusion,this review provides a detailed understanding of the GLA mechanism and presents multiple possibilities for the nutritional treatment of NAFLD.
基金supported by the China Postdoctoral Science Foun-dation(No.2023M730977)Henan Provincial Key Research and Development and Promotion of Special Project(No.232102110154)+3 种基金Henan Provincial Science and Technology Research Plan Joint fund Project(No.222103810062)Key R&D projects in Henan Province(No.231111113300)Double First-Class Discipline Construction Program of Henan University of Technology(No.0517-24410014)Cultivation Programme for Young Backbone Teachers in Henan University of Technology(No.21421230).
文摘A methionine-restricted diet(MRD)has been shown to reduce body fat deposition and improve obesityassociated metabolic disorders,with emerging evidence implicating the gut microbiota in mediating these effects.This study investigated the effects and underlying mechanisms of fecal microbiota transplantation(FMT)from MRD mouse donors on body fat deposition in obese mice.Five-week-old male C57BL/6J mice were continuously fed a high-fat diet(HFD,0.86%methionine+24%fat)to induce obesity,and then randomly divided into three groups.These groups received FMT from 23-week-old male C57BL/6J mice donors continuously fed with normal diet(0.86%methionine+4.2%fat),HFD,or MRD(0.17%methionine+24%fat)for eight weeks,respectively.Our results indicate that FMT from MRD mouse donors reduced body weight,plasma lipid levels,and percentage fat mass.Moreover,FMT from MRD mouse donors elevated the abundance of short-chain fatty acid(SCFA)-producing bacteria while decreasing pro-inflammatory bacteria levels in cecal contents.In addition,systemic metabolic processes were altered,featuring heightened SCFA production and enhanced energy metabolism.FMT from MRD mouse donors further facilitated fat catabolism,inhibited fat anabolism,and decreased lipid levels and lipid accumulation in liver.Meanwhile,FMT from MRD mouse donors also promoted fat browning,decreased adipocyte volume,and reduced fat deposition in adipose tissues.These findings underscore the potential of FMT from MRD mouse donors to improve the gut microbiota and reduce body fat deposition in obese mice.Importantly,our results elucidate that MRD intervention in obesity is,in part,mediated by regulating intestinal microbial composition.
